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Organism: Saccharomyces cerevisiae
Publication: Keck JM, Jones MH, Wong CC, Binkley J, Chen D, Jaspersen SL, Holinger EP, Xu T, Niepel M, Rout MP, Vogel J, Sidow A, Yates JR, Winey M., A Cell Cycle Phosphoproteome of the Yeast Centrosome, Science 24 June 2011: 1557-1561.
Comments: #18 Mitotic Prep4-TiO2 Phosphopeptide enrichment

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[View our MS interpretation guidelines.]
  Hit Protein Protein Desc Sequence Count Spectrum Count Sequence Coverage NSAF Mol. Wt.
[View Peptides]   SPC42 Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate att... 16 27 42.1% 0.167 42271
[View Peptides]   BFA1 Component of the GTPase-activating Bfa1p-Bub2p complex involved in multiple cell cycle checkpoint pa... 8 14 27.4% 0.0549 66087
[View Peptides]   NUD1 Component of the spindle pole body outer plaque, required for exit from mitosis 29 42 25.7% 0.111 94104
[View Peptides]   SPC29 Inner plaque spindle pole body (SPB) component, links the central plaque component Spc42p to the inn... 10 30 20.9% 0.267 29280
[View Peptides]   YIL002W-A Putative protein of unknown function; identified by expression profiling and mass spectrometry 2 3 18.8% 0.0979 7729
[View Peptides]   CNM67 Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclea... 8 12 17.4% 0.0465 67400
[View Peptides]   YLR445W Putative protein of unknown function; transcription is regulated by Ume6p and induced in response to... 1 1 14.4% 0.012 22365
[View Peptides]   PRE2 Beta 5 subunit of the 20S proteasome, responsible for the chymotryptic activity of the proteasome 1 1 14.3% 0.00784 31637
[View Peptides]   CDC31 Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication i... 1 1 11.8% 0.014 18751
[View Peptides]   RPL5 Protein component of the large (60S) ribosomal subunit with similarity to E. coli L18 and rat L5 rib... 1 1 11.1% 0.00758 33715
[View Peptides]   BBP1 Protein required for the spindle pole body (SPB) duplication, localized at the central plaque periph... 3 3 10.4% 0.0175 45384
[View Peptides]   SPC72 Component of the cytoplasmic Tub4p (gamma-tubulin) complex, binds spindle pole bodies and links them... 7 7 10.0% 0.0253 72105
[View Peptides]   SPC110 Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nu... 10 12 8.8% 0.0286 111782
[View Peptides]   HUA2 Cytoplasmic protein of unknown function; computational analysis of large-scale protein-protein inter... 1 1 8.6% 0.00926 28414
[View Peptides]   TUB4 Gamma-tubulin, involved in nucleating microtubules from both the cytoplasmic and nuclear faces of th... 1 1 7.8% 0.00476 52628
[View Peptides]   MLP2 Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the... 7 9 7.5% 0.0121 195140
[View Peptides]   KAR1 Essential protein involved in karyogamy during mating and in spindle pole body duplication during mi... 1 1 6.2% 0.0052 50653
[View Peptides]   NBP1 Spindle pole body (SPB) component, required for the insertion of the duplication plaque into the nuc... 1 1 5.3% 0.00706 37354
[View Peptides]   MDM34 Mitochondrial component of the ERMES complex that links the ER to mitochondria and may promote inter... 1 1 5.2% 0.0049 51990
[View Peptides]   SWA2 Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoatin... 1 1 5.1% 0.00337 75020
[View Peptides]   CDC14 Protein phosphatase required for mitotic exit; located in the nucleolus until liberated by the FEAR ... 1 1 4.7% 0.00408 61907
[View Peptides]   GVP36 BAR domain-containing protein that localizes to both early and late Golgi vesicles; required for ada... 1 1 4.6% 0.0069 36670
[View Peptides]   PHB1 Subunit of the prohibitin complex (Phb1p-Phb2p), a 1.2 MDa ring-shaped inner mitochondrial membrane ... 1 1 4.2% 0.00784 31427
[View Peptides]   IDP3 Peroxisomal NADP-dependent isocitrate dehydrogenase, catalyzes oxidation of isocitrate to alpha-keto... 1 2 3.3% 0.0107 47856
[View Peptides]   SPC97 Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the... 2 2 3.2% 0.00547 96825
[View Peptides]   YPL109C Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purifi... 1 1 3.2% 0.00343 76176
[View Peptides]   BUD8 Protein involved in bud-site selection; diploid mutants display a unipolar budding pattern instead o... 1 1 3.2% 0.00373 66289
[View Peptides]   MPS2 Essential membrane protein localized at the nuclear envelope and spindle pole body (SPB), required f... 1 3 2.8% 0.0174 44585
[View Peptides]   ALD6 Cytosolic aldehyde dehydrogenase, activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; re... 1 1 2.6% 0.0045 54414
[View Peptides]   CDC5 Polo-like kinase with similarity to Xenopus Plx1 and S. pombe Plo1p; found at bud neck, nucleus and ... 1 1 2.3% 0.00319 81031
[View Peptides]   PDC6 Minor isoform of pyruvate decarboxylase, decarboxylates pyruvate to acetaldehyde, involved in amino ... 1 1 2.3% 0.004 61581
[View Peptides]   PDC5 Minor isoform of pyruvate decarboxylase, key enzyme in alcoholic fermentation, decarboxylates pyruva... 1 1 2.3% 0.004 61912
[View Peptides]   PDC1 Major of three pyruvate decarboxylase isozymes, key enzyme in alcoholic fermentation, decarboxylates... 1 1 2.3% 0.004 61495
[View Peptides]   THI3 Probable alpha-ketoisocaproate decarboxylase, may have a role in catabolism of amino acids to long-c... 1 1 2.1% 0.0037 68366
[View Peptides]   SFI1 Centrin (Cdc31p)-binding protein required for spindle pole body (SPB) duplication, localizes to the ... 1 1 2.0% 0.00238 112978
[View Peptides]   Reverse_YPL195W APL5 SGDID:S000006116, Chr XVI from 176222-179020, Verified ORF, "Delta adaptin-like subunit of the ... 1 1 1.8% 0.00241 106924
[View Peptides]   NDC1 Nuclear envelope protein with multiple putative transmembrane domains, required for nuclear pore com... 1 1 1.7% 0.00344 74134
[View Peptides]   RIA1 Cytoplasmic GTPase involved in biogenesis of the 60S ribosome; has similarity to translation elongat... 1 1 1.4% 0.00203 124464
[View Peptides]   YME2 Integral inner mitochondrial membrane protein with a role in maintaining mitochondrial nucleoid stru... 1 1 0.9% 0.00265 96689
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