Protein Overview: RVS161

General Information:
Name(s) found:	RVS161 / YCR009C [SGD]
Description(s) found: SHOW ONLY BEST	pir\|\|S19516 RVS161 protein - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|6319854\|ref\|NP_009935.1\| Amphiphysin-like lipid raft protein; subunit of a complex (Rvs161p-Rvs167p) that regulates polarization of the actin cytoskeleton, endocytosis, cell polarity, cell fusion and viability following starvation or osmotic stress; Rv [NCBI NR] gi\|1907152\|emb\|CAA42326.1\| hypothetical protein [Saccharomyces cerevisiae] [NCBI NR] gi\|134128\|sp\|P25343.1\|RV161_YEAST RecName: Full=Reduced viability upon starvation protein 161 [NCBI NR] Reduced viability upon starvation protein 161 OS=Saccharomyces cerevisiae GN=RVS161 PE=1 SV=1 [Swiss-Prot] (P25343) Reduced viability upon starvation protein 161 [Swiss-Prot] RVS161 Protein involved in cell polarity development [mips] Amphiphysin-like lipid raft protein; interacts with Rvs167p and regulates polarization of the actin cytoskeleton, endocytosis, cell polarity, cell fusion and viability following starvation or osmotic stress [SGD] RVS161, Chr III from 130739-131536, reverse complement [yeast_orfs2] Chr III from 130739-131536, reverse complement [yeastorf3.fasta] Chr III from 131536-130739, reverse complement [yeastorf5.fasta] RVS161 SGDID:S0000602, Chr III from 131539-130742, reverse complement, Verified ORF [yeastflysequest.fasta] BAR adaptor protein, subunit of a complex (Rvs161p, Rvs167p) that regulates actin, endocytosis, and viability following starvation or osmotic stress [Saccharomyces cerevisiae]gi\|1907152\|emb\|CAA42326.1\| hypothetical protein [Saccharomyces cerevisiae]gi\|8 [nrdb_11152004_Chlamydomonas] RVS161 SGDID:S000000602, Chr III from 131539-130742, reverse complement, Verified ORF, "Amphiphysin-like lipid raft protein; subunit of a complex (Rvs161p-Rvs167p) that regulates polarization of the actin cytoskeleton, endocytosis, cell polarity, cell fus [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] RVS161 SGDID:S000000602, Chr III from 131540-130743, reverse complement, Verified ORF, "Amphiphysin-like lipid raft protein; subunit of a complex (Rvs161p-Rvs167p) that regulates polarization of the actin cytoskeleton, endocytosis, cell polarity, cell fus [yeast-contam.fasta] RVS161 SGDID:S000000602, Chr III from 131540-130743, reverse complement, Verified ORF, "Amphiphysin-like lipid raft protein; interacts with Rvs167p and regulates polarization of the actin cytoskeleton, endocytosis, cell polarity, cell fusion and viability [SGD_S-cerevisiae_na_05-08-2009_con_reversed.fasta] Amphiphysin-like lipid raft protein; subunit of a complex (Rvs161p-Rvs167p) that regulates polarization of the actin cytoskeleton, endocytosis, cell polarity, cell fusion and viability following starvation or osmotic stress; Rvs161p [Saccharomyces cerevis [nr-271106-contam.fasta] Reduced viability upon starvation protein 161 [nr-271106-contam.fasta] hypothetical protein [Saccharomyces cerevisiae] [nr-271106-contam.fasta] RVS161 SGDID:S000000602, Chr III from 131540-130743, reverse complement, Verified ORF, "Amphiphysin-like lipid raft protein; subunit of a complex (Rvs161p-Rvs167p) that regulates polarization of the actin cytoskeleton, endocytosis, cell polarity, cell fusion and viability following starvation or osmotic stress" [yeast-bovrabprots.fasta] RVS161 SGDID:S000000602, Chr III from 131540-130743, reverse complement, Verified ORF, "Amphiphysin-like lipid raft protein; interacts with Rvs167p and regulates polarization of the actin cytoskeleton, endocytosis, cell polarity, cell fusion and viability following starvation or osmotic stress" [scerevisihumanrev.fasta] [chemostatdb.fasta] Reduced viability upon starvation protein 161 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=RVS161 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] RVS161 SGDID:S000000602, Chr III from 131542-130745, Genome Release 64-1-1, reverse complement, Verified ORF, "Amphiphysin-like lipid raft protein; interacts with Rvs167p and regulates polarization of the actin cytoskeleton, endocytosis, cell polarity, cell fusion and viability following starvation or osmotic stress" [yeast-030211-contam.fasta] RVS161 SGDID:S000000602, Chr III from 131542-130745, Genome Release 64-2-1, reverse complement, Verified ORF, "Amphiphysin-like lipid raft protein; interacts with Rvs167p and regulates polarization of the actin cytoskeleton, endocytosis, cell polarity, cell fusion and viability following starvation or osmotic stress" [20141208_orf_trans.fasta] TR561\|c0_g1_i1\|g.336 ORF TR561\|c0_g1_i1\|g.336 TR561\|c0_g1_i1\|m.336 type:complete len:266 (+) TR561\|c0_g1_i1:85-882(+) [Y12_S_cerevisiae-Sep2015-contam.fasta] TR351\|c0_g1_i1\|g.277 ORF TR351\|c0_g1_i1\|g.277 TR351\|c0_g1_i1\|m.277 type:complete len:266 (+) TR351\|c0_g1_i1:100-897(+) [DBVPG6044_S_cerevisiae-Sep2015-contam.fasta] YCR009C SGDID:S000000602, Chr III from 131542-130745, Genome Release 64-1-1, reverse complement, Verified ORF, "Amphiphysin-like lipid raft protein; interacts with Rvs167p and regulates polarization of the actin cytoskeleton, endocytosis, cell polarity, cell fusion and viability following starvation or osmotic stress" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta] asmbl_9112\|g.6656 ORF asmbl_9112\|g.6656 asmbl_9112\|m.6656 type:complete len:266 (+) asmbl_9112:86-883(+) [Y12_Trinity_v2_2_0_PASA_6May2016-contam.fasta] ID=asmbl_9105\|g.5634 ORF ID=asmbl_9105\|g.5634 ID=asmbl_9105\|m.5634 type:complete len:266 (+) ID=asmbl_9105:87-884(+) [DBVPG6044_quiverGenomes_Trinity_v2_2_0_PASA_2June2016-contam.fasta] ID=asmbl_9119\|g.5589 ORF ID=asmbl_9119\|g.5589 ID=asmbl_9119\|m.5589 type:complete len:266 (+) ID=asmbl_9119:86-883(+) [Y12_quiver_Trinity_v2_2_0_PASA_2June2016-contam.fasta]
Organism:	Saccharomyces cerevisiae
Length:	265 amino acids
Gene Ontology:
Cellular Component:	mating projection [IDA] membrane raft [IDA] actin cortical patch [TAS]
Biological Process:	bipolar cellular bud site selection [IMP] response to osmotic stress [IMP] endocytosis [IMP]
Molecular Function:	cytoskeletal protein binding [IPI][ISS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSWEGFKKAI NRAGHSVIIK NVDKTIDKEY DMEERRYKVL QRAGEALQKE AKGFLDSLRA  60
   61 VTASQTTIAE VISNLYDDSK YVAGGGYNVG NYYLQCVQDF DSETVKQLDG PLRETVLDPI 120
  121 TKFSTYFKEI EEAIKKRDHK KQDFDAAKAK VRRLVDKPAK DASKLPRAEK ELSLAKDIFE 180
  181 NLNNQLKTEL PQLVSLRVPY FDPSFEALIK IQLRFCTDGY TRLAQIQQYL DQQSRDDYAN 240
  241 GLLDTKIEEL LGQMTSLDIC ALGIK

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		ABP1 BSP1 CMD1 CRN1 GYP5 INP52 LSP1 MLC1 NOP53 PIL1 RPF2 RVS161 RVS167 SAC6 SLM1 YNL208W
View Details	Krogan NJ, et al. (2006)		AIM3 BAR1 COA4 GYL1 GYP5 HNT1 KIN2 MEF2 NCP1 PCL10 PRP28 RVS161 RVS167 SAE2 SLK19 TIM21 VPS15
View Details	Gavin AC, et al. (2006)		RVS161 RVS167
View Details	Ho Y, et al. (2002)		ARG1 ARG4 AYR1 CDC33 COR1 CPR6 ERG27 GPT2 KGD2 KRS1 LIA1 LPD1 MAE1 MGE1 OM45 PCT1 PDA1 PET10 PET9 PIL1 PMI40 POR1 PRE10 PRO3 RET2 RNR2 RSP5 RTN2 RVB2 RVS161 RVS167 SEC18 SEC21 SEC26 SEC27 SEC28 SES1 TIF34 TIF35 TPA1 TRP5 YBP2 YNL181W
View Details	Qiu et al. (2008)		ABP1 AIP1 ARC40 BEM1 BUD6 CAP1 CAP2 COF1 IQG1 LAS17 PFY1 RVS161 RVS167 SAC6 SCP1 SLA1 SLA2 SPA2 SRV2 TPM1 TPM2 TWF1 VRP1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	NAF1	No Comments	Hazbun TR, et al. (2003)
View Run	YNL313C	No Comments	Hazbun TR, et al. (2003)
View Run	RLF2	cac1: TAP-tagged	Green EM, et al (2005)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	RVS167	RVS161	2	Uetz P, et al. (2000)
View Screen	SDS23	RVS161	1	Unpublished Fields Lab Data
View Screen	YHR022C	RVS161	1	Unpublished Fields Lab Data
View Screen	RED1	RVS161	1	Unpublished Fields Lab Data
View Screen	INP52	RVS161	1	Unpublished Fields Lab Data
View Screen	PDI1	RVS161	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	RVS161 and SAC6	Huh WK, et al. (2003)
View Data	RVS161	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..265]	PSI-BLAST	8.69897	Arfaptin, Rac-binding fragment

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2