Protein Overview: SIF2

General Information:
Name(s) found:	SIF2 / YBR103W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6319579\|ref\|NP_009661.1\| WD40 repeat-containing subunit of the Set3C histone deacetylase complex, which represses early/middle sporulation genes; antagonizes telomeric silencing; binds specifically to the Sir4p N-terminus; Sif2p [Saccharomyces cerevisi [NCBI NR] gi\|57014129\|sp\|P38262.2\|SIF2_YEAST RecName: Full=SIR4-interacting protein SIF2 [NCBI NR] gi\|51013557\|gb\|AAT93072.1\| YBR103W [Saccharomyces cerevisiae] [NCBI NR] gi\|1870107\|emb\|CAA85058.1\| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR] SIR4-interacting protein SIF2 OS=Saccharomyces cerevisiae GN=SIF2 PE=1 SV=2 [Swiss-Prot] SIF2 Sir4p interacting protein [mips] WD40 repeat-containing subunit of the Set3C histone deacetylase complex, which represses early/middle sporulation genes; antagonizes telomeric silencing; binds specifically to the Sir4p N-terminus [SGD] SIF2, Chr II from 447668-449275 [yeast_orfs2] Chr II from 447668-449275 [yeastorf3.fasta] Chr II from 447667-449274 [yeastorf4.fasta] Chr II from 447665-449272 [yeastorf5.fasta] SIF2 SGDID:S0000307, Chr II from 447666-449273, Verified ORF [yeastflysequest.fasta] SIF2 SGDID:S0000307, Chr II from 447703-449310, Verified ORF [10112005cervSGD.fasta] Sif2p [Saccharomyces cerevisiae]gi\|51013557\|gb\|AAT93072.1\| YBR103W [Saccharomyces cerevisiae]gi\|1870107\|emb\|CAA85058.1\| unnamed protein product [Saccharomyces cerevisiae] [nrdb_11152004_Chlamydomonas] SIF2 SGDID:S000000307, Chr II from 447703-449310, Verified ORF, "WD40 repeat-containing subunit of the Set3C histone deacetylase complex, which represses early/middle sporulation genes; antagonizes telomeric silencing; binds specifically to the Sir4p N-te [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] SIR4-interacting protein SIF2 [nr-271106-contam.fasta] WD40 repeat-containing subunit of the Set3C histone deacetylase complex, which represses early/middle sporulation genes; antagonizes telomeric silencing; binds specifically to the Sir4p N-terminus; Sif2p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] YBR103W [Saccharomyces cerevisiae] [nr-271106-contam.fasta] unnamed protein product [Saccharomyces cerevisiae] [nr-271106-contam.fasta] SIF2 SGDID:S000000307, Chr II from 447703-449310, Verified ORF, "WD40 repeat-containing subunit of the Set3C histone deacetylase complex, which represses early/middle sporulation genes; antagonizes telomeric silencing; binds specifically to the Sir4p N-terminus" [yeast-bovrabprots.fasta] [chemostatdb.fasta] SIR4-interacting protein SIF2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=SIF2 PE=1 SV=2 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] SIF2 SGDID:S000000307, Chr II from 447709-449316, Genome Release 64-1-1, Verified ORF, "WD40 repeat-containing subunit of the Set3C histone deacetylase complex, which represses early/middle sporulation genes; antagonizes telomeric silencing; binds specifically to the Sir4p N-terminus" [yeast-030211-contam.fasta] SIF2 SGDID:S000000307, Chr II from 447709-449316, Genome Release 64-2-1, Verified ORF, "WD40 repeat-containing subunit of Set3C histone deacetylase complex; complex represses early/middle sporulation genes; antagonizes telomeric silencing; binds specifically to the Sir4p N-terminus" [20141208_orf_trans.fasta] YBR103W SGDID:S000000307, Chr II from 447709-449316, Genome Release 64-1-1, Verified ORF, "WD40 repeat-containing subunit of the Set3C histone deacetylase complex, which represses early/middle sporulation genes; antagonizes telomeric silencing; binds specifically to the Sir4p N-terminus" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	535 amino acids
Gene Ontology:
Cellular Component:	nucleus [IPI] histone deacetylase complex [IPI]
Biological Process:	negative regulation of transcription from RNA polymerase II promoter [IPI] histone deacetylation [IDA][IPI] chromatin silencing at telomere [IGI][IPI] negative regulation of meiosis [IPI]
Molecular Function:	transcription corepressor activity [IDA] NAD-dependent histone deacetylase activity [IPI] NAD-independent histone deacetylase activity [IPI]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSITSEELNY LIWRYCQEMG HEVSALALQD ETRVLEFDEK YKEHIPLGTL VNLVQRGILY  60
   61 TESELMVDSK GDISALNEHH LSEDFNLVQA LQIDKEKFPE ISSEGRFTLE TNSESNKAGE 120
  121 DGASTVERET QEDDTNSIDS SDDLDGFVKI LKEIVKLDNI VSSTWNPLDE SILAYGEKNS 180
  181 VARLARIVET DQEGKKYWKL TIIAELRHPF ALSASSGKTT NQVTCLAWSH DGNSIVTGVE 240
  241 NGELRLWNKT GALLNVLNFH RAPIVSVKWN KDGTHIISMD VENVTILWNV ISGTVMQHFE 300
  301 LKETGGSSIN AENHSGDGSL GVDVEWVDDD KFVIPGPKGA IFVYQITEKT PTGKLIGHHG 360
  361 PISVLEFNDT NKLLLSASDD GTLRIWHGGN GNSQNCFYGH SQSIVSASWV GDDKVISCSM 420
  421 DGSVRLWSLK QNTLLALSIV DGVPIFAGRI SQDGQKYAVA FMDGQVNVYD LKKLNSKSRS 480
  481 LYGNRDGILN PLPIPLYASY QSSQDNDYIF DLSWNCAGNK ISVAYSLQEG SVVAI

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		CPR1 HOS2 HOS4 SET3 SIF2 SNT1
View Details	Krogan NJ, et al. (2006)		AKL1 HOS4 HST1 RFM1 SET3 SIF2 SNT1 SUM1
View Details	Gavin AC, et al. (2006)		CPR1 HOS2 HOS4 SET3 SIF2 SNT1
View Details	Gavin AC, et al. (2002)		BLM10 CDC55 CIN1 CPR1 ERG13 GUS1 HHF1, HHF2 HOS2 HOS4 HST1 IML1 KAP95 KEL1 LTE1 MYO5 PFK1 PPH21 PPH22 PRE1 PRE10 PRE2 PRE4 PRE5 PRE6 PRE7 PRE8 PRE9 PUP1 PUP2 PUP3 RRD2 RTS1 RTS3 RVB2 SCL1 SET3 SIF2 SNF2 SNT1 SRP1 TDH2 TDH3 TEF4 TPD3 YBL104C YOR1 YRA1 ZDS1 ZDS2
View Details	Ho Y, et al. (2002)		AVT2 DIS3 EAF3 FIP1 HAS1 HOS4 HPR1 KAP95 MES1 MFT1 NAM8 NOP6 NUP1 NUP2 NUP60 PAP1 PCT1 REB1 RNT1 RRP4 RRP43 RRP6 RTT103 SIF2 SIN3 SNT1 SNU56 SRP1 STO1 TFP1 THO2 TRA1 TRM3 UME1 UTP21 YMR155W YPR090W YRF1-3, YRF1-7 ZDS2
View Details	Qiu et al. (2008)		HDA3 HOS2 HOS4 HST1 RPD3 RXT2 SDS3 SET3 SIF2 SNT1 STB2

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	MLP2	#29 Asynchronous Prep5-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#12 Mitotic Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	HSH49	SIF2	2	Uetz P, et al. (2000)

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	SIF2	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..187]	MSA	4.514997	View MSA. No confident structure predictions are available.
2	View Details	[188..535]	PSI-BLAST	130.29073	beta1-subunit of the signal-transducing G protein heterotrimer

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2