Protein Overview: PMA2

General Information:
Name(s) found:	PMA2 / YPL036W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6325221\|ref\|NP_015289.1\| Plasma membrane H+-ATPase, isoform of Pma1p, involved in pumping protons out of the cell; regulator of cytoplasmic pH and plasma membrane potential; Pma2p [Saccharomyces cerevisiae] [NCBI NR] pir\|\|PXBY2P H+-exporting ATPase (EC 3.6.3.6) 2, plasma membrane - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|1709667\|sp\|P19657.3\|PMA2_YEAST RecName: Full=Plasma membrane ATPase 2; AltName: Full=Proton pump 2 [NCBI NR] gi\|1171422\|gb\|AAB68184.1\| Pma2p: Plasma membrane ATPase [Saccharomyces cerevisiae] [NCBI NR] Plasma membrane ATPase 2 OS=Saccharomyces cerevisiae GN=PMA2 PE=1 SV=3 [Swiss-Prot] PMA2 Poorly expressed, H+-transporting P-type ATPase [mips] Plasma membrane H+-ATPase, isoform of Pma1p, involved in pumping protons out of the cell; regulator of cytoplasmic pH and plasma membrane potential [SGD] (P19657) Plasma membrane ATPase 2 (EC 3.6.3.6) (Proton pump 2) [4932.FASTACCERV.txt] Chr XVI from 482839-485682 [hmr_yeast5_DM3-1_pombe.fasta] PMA2, Chr XVI from 482839-485682 [yeast_orfs2] PMA2 SGDID:S0005957, Chr XVI from 482839-485682, Verified ORF [yeastflysequest.fasta] PMA2 SGDID:S0005957, Chr XVI from 482841-485684, Verified ORF [10112005cervSGD.fasta] Pma2p [Saccharomyces cerevisiae]gi\|2144522\|pir\|\|PXBY2P H+-exporting ATPase (EC 3.6.3.6) 2, plasma membrane - yeast (Saccharomyces cerevisiae)gi\|1171422\|gb\|AAB68184.1\| Pma2p: Plasma membrane ATPase [Saccharomyces cerevisiae]gi\|1709667\|sp\|P19657\|PMA2_YE [nrdb_11152004_Chlamydomonas] PMA2 SGDID:S000005957, Chr XVI from 482841-485684, Verified ORF, "Plasma membrane H+-ATPase, isoform of Pma1p, involved in pumping protons out of the cell; regulator of cytoplasmic pH and plasma membrane potential" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] Pma2p: Plasma membrane ATPase [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Plasma membrane ATPase 2 (Proton pump 2) [nr-271106-contam.fasta] Plasma membrane H+-ATPase, isoform of Pma1p, involved in pumping protons out of the cell; regulator of cytoplasmic pH and plasma membrane potential; Pma2p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] [chemostatdb.fasta] Plasma membrane ATPase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PMA2 PE=1 SV=3 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] PMA2 SGDID:S000005957, Chr XVI from 482843-485686, Genome Release 64-1-1, Verified ORF, "Plasma membrane H+-ATPase, isoform of Pma1p, involved in pumping protons out of the cell; regulator of cytoplasmic pH and plasma membrane potential" [yeast-030211-contam.fasta] PMA2 SGDID:S000005957, Chr XVI from 482843-485686, Genome Release 64-2-1, Verified ORF, "Plasma membrane H+-ATPase; isoform of Pma1p, involved in pumping protons out of the cell; regulator of cytoplasmic pH and plasma membrane potential" [20141208_orf_trans.fasta] YPL036W SGDID:S000005957, Chr XVI from 482843-485686, Genome Release 64-1-1, Verified ORF, "Plasma membrane H+-ATPase, isoform of Pma1p, involved in pumping protons out of the cell; regulator of cytoplasmic pH and plasma membrane potential" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	947 amino acids
Gene Ontology:
Cellular Component:	mitochondrion [IDA] plasma membrane [IDA]
Biological Process:	proton transport [IDA] regulation of pH [IEP]
Molecular Function:	hydrogen-exporting ATPase activity, phosphorylative mechanism [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSSTEAKQYK EKPSKEYLHA SDGDDPANNS AASSSSSSST STSASSSAAA VPRKAAAASA  60
   61 ADDSDSDEDI DQLIDELQSN YGEGDESGEE EVRTDGVHAG QRVVPEKDLS TDPAYGLTSD 120
  121 EVARRRKKYG LNQMAEENES LIVKFLMFFV GPIQFVMEAA AILAAGLSDW VDVGVICALL 180
  181 LLNASVGFIQ EFQAGSIVDE LKKTLANTAT VIRDGQLIEI PANEVVPGEI LQLESGTIAP 240
  241 ADGRIVTEDC FLQIDQSAIT GESLAAEKHY GDEVFSSSTV KTGEAFMVVT ATGDNTFVGR 300
  301 AAALVGQASG VEGHFTEVLN GIGIILLVLV IATLLLVWTA CFYRTVGIVS ILRYTLGITI 360
  361 IGVPVGLPAV VTTTMAVGAA YLAKKQAIVQ KLSAIESLAG VEILCSDKTG TLTKNKLSLH 420
  421 EPYTVEGVSP DDLMLTACLA ASRKKKGLDA IDKAFLKSLI EYPKAKDALT KYKVLEFHPF 480
  481 DPVSKKVTAV VESPEGERIV CVKGAPLFVL KTVEEDHPIP EDVHENYENK VAELASRGFR 540
  541 ALGVARKRGE GHWEILGVMP CMDPPRDDTA QTINEARNLG LRIKMLTGDA VGIAKETCRQ 600
  601 LGLGTNIYNA ERLGLGGGGD MPGSELADFV ENADGFAEVF PQHKYRVVEI LQNRGYLVAM 660
  661 TGDGVNDAPS LKKADTGIAV EGATDAARSA ADIVFLAPGL SAIIDALKTS RQIFHRMYSY 720
  721 VVYRIALSLH LEIFLGLWIA ILNNSLDINL IVFIAIFADV ATLTIAYDNA PYAPEPVKWN 780
  781 LPRLWGMSII LGIVLAIGSW ITLTTMFLPN GGIIQNFGAM NGVMFLQISL TENWLIFVTR 840
  841 AAGPFWSSIP SWQLAGAVFA VDIIATMFTL FGWWSENWTD IVSVVRVWIW SIGIFCVLGG 900
  901 FYYIMSTSQA FDRLMNGKSL KEKKSTRSVE DFMAAMQRVS TQHEKSS

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	(MIPS) Mewes HW, et al. (2004)		PMA1 PMA2 PMP1 PMP2
View Details	Ho Y, et al. (2002)		ADR1 BBC1 CDC39 CDC53 CRM1 DUR1,2 ECM29 ECM33 FAA4 GAL3 GCN1 GLC7 GPA2 GUS1 HRT1 HXT6 HYP2 KOG1 MDN1 MKT1 MMS1 MYO2 NET1 NOP6 NSR1 PFK1 PMA1 PMA2 PPZ2 REG1 RPA190 RPN1 RSE1 RVB1 SDS22 SNF4 TPS1 UBI4 VPS13 YAR009C YGP1 YGR130C YLR035C-A
View Details	Qiu et al. (2008)		ATP6 NHA1 PMA1 PMA2 PMP2 VMA9

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	TOR1	No Comments	Reinke A, et al. (2004)
View Run	KOG1	No Comments	Reinke A, et al. (2004)
View Run	RLF2	cac1: TAP-tagged	Green EM, et al (2005)
View Run	DAD1	Dam1-765 complex purified from yeast with Dad1-TAP	Shimogawa MM, et al. (2006)
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	RLF2	phosphorylation data - cascade search	Green EM, et al (2005)
View Run	RLF2	identification data - first search in cascade	Green EM, et al (2005)
View Run	ASF1	sample 3928 - Asf1-S-TEV-ZZ (in hir3delta mutant)	Green EM, et al (2005)
View Run	ZDS1	Sample 2- cpn2 from june 2005	McCusker D, et al (2007)
View Run	ZDS1	Sample 3 - cpn + atp, from june 2005	McCusker D, et al (2007)
View Run	BOI1	Sample boi 1 with ha tag from october 2005	McCusker D, et al (2007)
View Run	BOI1	Sample boi1 with gst from october 2005	McCusker D, et al (2007)
View Run	MLP2	#26 Asynchronous Prep4-TiO2 Phosphopeptide enriched, Steps1-2	Keck JM, et al. (2011)
View Run	MLP2	#22 Asynchronous Prep2-IMAC Phosphopeptide enrichment B1-2	Keck JM, et al. (2011)
View Run	MLP2	#04 Alpha Factor Prep2	Keck JM, et al. (2011)
View Run	MLP2	#03 Alpha Factor Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#29 Asynchronous Prep5-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#06 Alpha Factor Prep3-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#08 Alpha Factor Prep4-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	RAD6	PMA2	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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No microscopy data found in the PDR for this protein.

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..37] [417..562]	PSI-BLAST	11000.0	Proton ATPase
2	View Details	[38..136] [195..308]	PSI-BLAST	11000.0	Proton ATPase
3	View Details	[137..194] [309..373] [713..905]	PSI-BLAST	11000.0	Proton ATPase
4	View Details	[374..416] [563..712] [906..947]	PSI-BLAST	11000.0	Proton ATPase

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

[View Details]

Source: Reynolds et al. 2008. Manuscript submitted

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2