Protein Overview: LAS17

General Information:
Name(s) found:	LAS17 / YOR181W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6324755\|ref\|NP_014824.1\| Actin assembly factor, activates the Arp2/3 protein complex that nucleates branched actin filaments; localizes with the Arp2/3 complex to actin patches; homolog of the human Wiskott-Aldrich syndrome protein (WASP); Las17p [Sacc [NCBI NR] gi\|2498506\|sp\|Q12446.1\|LAS17_YEAST RecName: Full=Proline-rich protein LAS17 [NCBI NR] pir\|\|S62057 proline-rich protein LAS17 - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|1420437\|emb\|CAA99390.1\| LAS17 [Saccharomyces cerevisiae] [NCBI NR] gi\|1101757\|dbj\|BAA11386.1\| prolin rich protein [Saccharomyces cerevisiae] [NCBI NR] Proline-rich protein LAS17 OS=Saccharomyces cerevisiae GN=LAS17 PE=1 SV=1 [Swiss-Prot] (Q12446) Proline-rich protein LAS17 [Swiss-Prot] LAS17 Component of actin cortical patches [mips] Actin assembly factor, activates the Arp2/3 protein complex that nucleates branched actin filaments; localizes with the Arp2/3 complex to actin patches; homolog of the human Wiskott-Aldrich syndrome protein (WASP) [SGD] Chr XV from 675939-677840 [hmr_yeast5_DM3-1_pombe.fasta] Chr XV from 675938-677839 [yeastorf3.fasta] LAS17, Chr XV from 675938-677839 [yeast_orfs2] LAS17 SGDID:S0005707, Chr XV from 675939-677840, Verified ORF [yeastflysequest.fasta] Actin assembly factor, activates the Arp2/3 protein complex that nucleates branched actin filaments; localizes with the Arp2/3 complex to actin patches; homolog of the human Wiskott-Aldrich syndrome protein (WASP) [Saccharomyces cerevisiae]gi\|1420437\|emb [nrdb_11152004_Chlamydomonas] LAS17 SGDID:S000005707, Chr XV from 675939-677840, Verified ORF, "Actin assembly factor, activates the Arp2/3 protein complex that nucleates branched actin filaments; localizes with the Arp2/3 complex to actin patches; homolog of the human Wiskott-Aldrich [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] LAS17 SGDID:S000005707, Chr XV from 675940-677841, Verified ORF, "Actin assembly factor, activates the Arp2/3 protein complex that nucleates branched actin filaments; localizes with the Arp2/3 complex to actin patches; homolog of the human Wiskott-Aldrich [yeast-contam.fasta] LAS17 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Proline-rich protein LAS17 [nr-271106-contam.fasta] Actin assembly factor, activates the Arp2/3 protein complex that nucleates branched actin filaments; localizes with the Arp2/3 complex to actin patches; homolog of the human Wiskott-Aldrich syndrome protein (WASP); Las17p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] prolin rich protein [Saccharomyces cerevisiae] [nr-271106-contam.fasta] LAS17 SGDID:S000005707, Chr XV from 675940-677841, Verified ORF, "Actin assembly factor, activates the Arp2/3 protein complex that nucleates branched actin filaments; localizes with the Arp2/3 complex to actin patches; homolog of the human Wiskott-Aldrich syndrome protein (WASP)" [yeast-bovrabprots.fasta] [chemostatdb.fasta] Proline-rich protein LAS17 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=LAS17 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] LAS17 SGDID:S000005707, Chr XV from 675939-677840, Genome Release 64-1-1, Verified ORF, "Actin assembly factor, activates the Arp2/3 protein complex that nucleates branched actin filaments; localizes with the Arp2/3 complex to actin patches; homolog of the human Wiskott-Aldrich syndrome protein (WASP)" [yeast-030211-contam.fasta] LAS17 SGDID:S000005707, Chr XV from 675939-677840, Genome Release 64-2-1, Verified ORF, "Actin assembly factor; C-terminal WCA domain activates Arp2/3 complex-mediated nucleation of branched actin filaments and a polyproline domain which can nucleate actin filaments independent of Arp2/3; mutants are defective in actin cytoskeleton dependent processes such as: endocytosis, bud site selection and cytokinesis; localizes with the Arp2/3 complex to actin cortical patches; homolog of the Wiskott-Aldrich Syndrome protein (WASP), implicated in severe immunodeficiency" [20141208_orf_trans.fasta] YOR181W SGDID:S000005707, Chr XV from 675939-677840, Genome Release 64-1-1, Verified ORF, "Actin assembly factor, activates the Arp2/3 protein complex that nucleates branched actin filaments; localizes with the Arp2/3 complex to actin patches; homolog of the human Wiskott-Aldrich syndrome protein (WASP)" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	633 amino acids
Gene Ontology:
Cellular Component:	cytoplasm [IDA] actin cortical patch [IDA ]
Biological Process:	bipolar cellular bud site selection [IMP] actin polymerization or depolymerization [IDA] response to osmotic stress [IMP] cytokinesis [IMP] actin filament organization [IDA] endocytosis [IMP]
Molecular Function:	cytoskeletal protein binding [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MGLLNSSDKE IIKRALPKAS NKIIDVTVAR LYIAYPDKNE WQYTGLSGAL ALVDDLVGNT  60
   61 FFLKLVDING HRGVIWDQEL YVNFEYYQDR TFFHTFEMEE CFAGLLFVDI NEASHFLKRV 120
  121 QKRERYANRK TLLNKNAVAL TKKVREEQKS QVVHGPRGES LIDNQRKRYN YEDVDTIPTT 180
  181 KHKAPPPPPP TAETFDSDQT SSFSDINSTT ASAPTTPAPA LPPASPEVRK EETHPKHSLP 240
  241 PLPNQFAPLP DPPQHNSPPQ NNAPSQPQSN PFPFPIPEIP STQSATNPFP FPVPQQQFNQ 300
  301 APSMGIPQQN RPLPQLPNRN NRPVPPPPPM RTTTEGSGVR LPAPPPPPRR GPAPPPPPHR 360
  361 HVTSNTLNSA GGNSLLPQAT GRRGPAPPPP PRASRPTPNV TMQQNPQQYN NSNRPFGYQT 420
  421 NSNMSSPPPP PVTTFNTLTP QMTAATGQPA VPLPQNTQAP SQATNVPVAP PPPPASLGQS 480
  481 QIPQSAPSAP IPPTLPSTTS AAPPPPPAFL TQQPQSGGAP APPPPPQMPA TSTSGGGSFA 540
  541 ETTGDAGRDA LLASIRGAGG IGALRKVDKS QLDKPSVLLQ EARGESASPP AAAGNGGTPG 600
  601 GPPASLADAL AAALNKRKTK VGAHDDMDNG DDW

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		BZZ1 LAS17 SLA1 VRP1
View Details	(MIPS) Mewes HW, et al. (2004)		ABP1 AIP1 ARC40 ARP2 BEM1 BUD6 CAP1 CAP2 COF1 CRN1 DLD2 GLK1 LAS17 OYE2 PAN1 PFY1 RVS167 SAC6 SLA1 SLA2 SRV2 TPM1 TPM2 TWF1 VRP1
View Details	Krogan NJ, et al. (2006)		BZZ1 LAS17 MEC1 SLA1 VRP1
View Details	Gavin AC, et al. (2006)		LAS17 LSB3 SLA1
View Details	Gavin AC, et al. (2002)		BSP1 BZZ1 CHC1 ECM25 END3 INP52 LAS17 LSB3 RAD51 SLA1 SLA2 STM1 SYP1 TFP1 VRP1 YPT7 YSC84
View Details	Ho Y, et al. (2002)		AAC3 BZZ1 GAL2 HXT6 HXT7 LAS17 MYO4 PEP1 PHO84 RPN1 RPN12 RVS167 SLA1 SQT1 VMA6 VRP1 YHM2 YNR065C
View Details	Qiu et al. (2008)		ABP1 ACT1 AIP1 ARC40 ARP2 BBC1 BEM1 BNR1 BSP1 BUD6 BZZ1 CAP1 CAP2 COF1 CRN1 ENT1 IQG1 LAS17 MYO5 PAN1 PEA2 PFY1 RVS161 RVS167 SAC6 SCD5 SLA1 SLA2 SPA2 SRV2 TPM1 TPM2 TWF1 VRP1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	ALG13	No Comments	Hazbun TR, et al. (2003)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	PRP40	LAS17	2	Unpublished Fields Lab Data
View Screen	LAS17	RVS167	2	Drees BL, et al. (2001)
View Screen	LAS17	ACT1	2	Drees BL, et al. (2001)
View Screen	LAS17	NSG1	2	Drees BL, et al. (2001)
View Screen	LAS17	SNA3	2	Unpublished Fields Lab Data
View Screen	LAS17	RBD2	2	Drees BL, et al. (2001)

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	LAS17	Huh WK, et al. (2003)
View Data	LAS17 and SAC6	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..154]	Pfam	49.455932	WH1 domain No confident structure predictions are available.
2	View Details	[155..426]	MSA	9.265997	View MSA. No confident structure predictions are available.
3	View Details	[427..633]	ORFEUS	11.97	Fibrinogen
4	View Details	[525..633]	MSA	4.180991	View MSA. No confident structure predictions are available.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2