Protein Overview: SKI7

General Information:
Name(s) found:	SKI7 / YOR076C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|74676474\|sp\|Q08491.1\|SKI7_YEAST RecName: Full=Superkiller protein 7 [NCBI NR] gi\|6324650\|ref\|NP_014719.1\| Putative GTPase that mediates interactions via its N-terminus between the exosome and Ski complex (Ski2p, Ski3p, Ski8p) which operate in the 3'-to-5' mRNA-decay pathway; cytoplasmic protein required for degrading nonstop mRNAs; [NCBI NR] pir\|\|S66959 hypothetical protein YOR076c - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|2104888\|emb\|CAA94561.1\| YOR29-27 [Saccharomyces cerevisiae] [NCBI NR] gi\|1420233\|emb\|CAA99269.1\| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR] Superkiller protein 7 OS=Saccharomyces cerevisiae GN=SKI7 PE=1 SV=1 [Swiss-Prot] SKI7 GTPase with function in 3`-to-5` degradation of mRNA [mips] Coupling protein that mediates interactions between the Ski complex and the cytoplasmic exosome during 3'-5' RNA degradation; eRF3-like domain targets nonstop mRNA for degradation; null mutants have superkiller phenotype [SGD] >Q08491 (Q08491) Chromosome XV reading frame ORF YOR076C [4932.FASTACCERV.txt] Chr XV from 471621-469378, reverse complement [hmr_yeast5_DM3-1_pombe.fasta] Chr XV from 469378-471621, reverse complement [yeastorf3.fasta] SKI7, Chr XV from 469378-471621, reverse complement [yeast_orfs2] SKI7 SGDID:S0005602, Chr XV from 471621-469378, reverse complement, Verified ORF [yeastflysequest.fasta] Antiviral adaptor protein that mediates interactions via its N-terminus between the exosome and Ski complex (Ski2p, Ski3p, Ski8p) which operate in the 3'-to-5' mRNA-decay pathway; cytoplasmic protein required for degrading nonstop mRNAs [Saccharomyces cer [nrdb_11152004_Chlamydomonas] (Q08491) S.cerevisiae chromosome XV reading frame ORF YOR076c (YOR29-27 protein) [cerv4932.FASTAC] SKI7 SGDID:S000005602, Chr XV from 471621-469378, reverse complement, Verified ORF, "Antiviral adaptor protein that mediates interactions via its N-terminus between the exosome and Ski complex (Ski2p, Ski3p, Ski8p) which operate in the 3'-to-5' mRNA-decay [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] SKI7 SGDID:S000005602, Chr XV from 471622-469379, reverse complement, Verified ORF, "Antiviral adaptor protein that mediates interactions via its N-terminus between the exosome and Ski complex (Ski2p, Ski3p, Ski8p) which operate in the 3'-to-5' mRNA-decay [yeast-contam.fasta] SKI7 SGDID:S000005602, Chr XV from 471622-469379, reverse complement, Verified ORF, "Putative GTPase that mediates interactions via its N-terminus between the exosome and Ski complex (Ski2p, Ski3p, Ski8p) which operate in the 3'-to-5' mRNA-decay pathway; [SGD_S-cerevisiae_na_08-19-2008_con_reversed.fasta] SKI7 SGDID:S000005602, Chr XV from 471622-469379, reverse complement, Verified ORF, "Coupling protein that mediates interactions between the Ski complex and the cytoplasmic exosome during 3'-5' RNA degradation; eRF3-like domain targets nonstop mRNA for de [SGD_S-cerevisiae_na_05-08-2009_con_reversed.fasta] YOR29-27 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] unnamed protein product [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Antiviral adaptor protein that mediates interactions via its N-terminus between the exosome and Ski complex (Ski2p, Ski3p, Ski8p) which operate in the 3'-to-5' mRNA-decay pathway; cytoplasmic protein required for degrading nonstop mRNAs; Ski7p [Saccharomy [nr-271106-contam.fasta] SKI7 SGDID:S000005602, Chr XV from 471622-469379, reverse complement, Verified ORF, "Putative GTPase that mediates interactions via its N-terminus between the exosome and Ski complex (Ski2p, Ski3p, Ski8p) which operate in the 3'-to-5' mRNA-decay pathway; cytoplasmic protein required for degrading nonstop mRNAs" [yeast-bovrabprots.fasta] SKI7 SGDID:S000005602, Chr XV from 471622-469379, reverse complement, Verified ORF, "Coupling protein that mediates interactions between the Ski complex and the cytoplasmic exosome during 3'-5' RNA degradation; eRF3-like domain targets nonstop mRNA for degradation; null mutants have superkiller phenotype" [scerevisihumanrev.fasta] SKI7 SGDID:S000005602, Chr XV from 471622-469379, reverse complement, Verified ORF, "Antiviral adaptor protein that mediates interactions via its N-terminus between the exosome and Ski complex (Ski2p, Ski3p, Ski8p) which operate in the 3'-to-5' mRNA-decay pathway; cytoplasmic protein required for degrading nonstop mRNAs" [yeast-contam_custom.fasta] [chemostatdb.fasta] Superkiller protein 7 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=SKI7 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] SKI7 SGDID:S000005602, Chr XV from 471620-469377, Genome Release 64-1-1, reverse complement, Verified ORF, "Coupling protein that mediates interactions between the Ski complex and the cytoplasmic exosome during 3'-5' RNA degradation; eRF3-like domain targets nonstop mRNA for degradation; null mutants have superkiller phenotype" [yeast-030211-contam.fasta] SKI7 SGDID:S000005602, Chr XV from 471620-469377, Genome Release 64-2-1, reverse complement, Verified ORF, "Coupling protein for the Ski complex and cytoplasmic exosome; involved in 3'-5' RNA degradation; eRF3-like domain targets nonstop mRNA for degradation; null mutants have superkiller phenotype; SKI7 has a paralog, HBS1, that arose from the whole genome duplication" [20141208_orf_trans.fasta] YOR076C SGDID:S000005602, Chr XV from 471620-469377, Genome Release 64-1-1, reverse complement, Verified ORF, "Coupling protein that mediates interactions between the Ski complex and the cytoplasmic exosome during 3'-5' RNA degradation; eRF3-like domain targets nonstop mRNA for degradation; null mutants have superkiller phenotype" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	747 amino acids
Gene Ontology:
Cellular Component:	cytoplasm [IDA] exosome (RNase complex) [IDA]
Biological Process:	response to exogenous dsRNA [IMP] mRNA catabolic process [IDA]
Molecular Function:	protein binding [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSLLEQLARK RIEKSKGLLS ADQSHSTSKS ASLLERLHKN RETKDNNAET KRKDLKTLLA  60
   61 KDKVKRSDFT PNQHSVSLSL KLSALKKSNS DLEKQGKSVT LDSKENELPT KRKSPDDKLN 120
  121 LEESWKAIKE MNHYCFLKND PCINQTDDFA FTNFIIKDKK NSLSTSIPLS SQNSSFLSLK 180
  181 KHNNELLGIF VPCNLPKTTR KVAIENFNRP SPDDIIQSAQ LNAFNEKLEN LNIKSVPKAE 240
  241 KKEPINLQTP PTESIDIHSF IATHPLNLTC LFLGDTNAGK STLLGHLLYD LNEISMSSMR 300
  301 ELQKKSSNLD PSSSNSFKVI LDNTKTEREN GFSMFKKVIQ VENDLLPPSS TLTLIDTPGS 360
  361 IKYFNKETLN SILTFDPEVY VLVIDCNYDS WEKSLDGPNN QIYEILKVIS YLNKNSACKK 420
  421 HLIILLNKAD LISWDKHRLE MIQSELNYVL KENFQWTDAE FQFIPCSGLL GSNLNKTENI 480
  481 TKSKYKSEFD SINYVPEWYE GPTFFSQLYL LVEHNMNKIE TTLEEPFVGT ILQSSVLQPI 540
  541 AEINYVSLKV LINSGYIQSG QTIEIHTQYE DFHYYGIVSR MKNSKQILET NTKNNISVGL 600
  601 NPDILEVLVK IHNTEDFTKK QFHIRKGDII IHSRKTNTLS PNLPNTLKLL ALRLIKLSIQ 660
  661 THALSDPVDL GSELLLYHNL THNAVKLVKI LGTNDISINP NQSLIVEVEI IEPDFALNVI 720
  721 DSKYITNNIV LTSIDHKVIA VGRIACQ

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		CSL4 DIS3 LRP1 MPP6 MTR3 RRP4 RRP40 RRP42 RRP43 RRP45 RRP46 RRP6 SKI3 SKI6 SKI7 SRP1
View Details	Krogan NJ, et al. (2006)		CSL4 DDI1 DIS3 ENT3 LRP1 MPP6 MTR3 OSM1 RRP4 RRP40 RRP42 RRP43 RRP45 RRP46 RRP6 SKI6 SKI7 YBL055C
View Details	Gavin AC, et al. (2006)		CSL4 DIS3 MTR3 RRP4 RRP40 RRP42 RRP43 RRP45 RRP46 RRP6 SKI6 SKI7
View Details	Gavin AC, et al. (2002)		CMD1 CSL4 CTR9 DIS3 ECM16 GCN1 LRP1 MAM33 MLC1 MTR3 MYO2 NMD5 RRP4 RRP40 RRP42 RRP43 RRP45 RRP46 RRP6 SAM1 SHE4 SKI2 SKI3 SKI6 SKI7 SRP1 UTP10 UTP20 UTP22 YIR035C

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	MLP2	#32 Asynchronous Prep-No Phosphopeptide enrichment	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	IMP4	SKI7	2	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	SKI7	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..70]	deduced	N/A	No confident structure predictions are available.
2	View Details	[71..260]	MSA	5.516997	View MSA. No confident structure predictions are available.
3	View Details	[261..293] [337..516]	PSI-BLAST	1110.0	Elongation factor eEF-1alpha, domain 2; Elongation factor eEF-1alpha, C-terminal domain; Elongation factor eEF-1alpha, N-terminal (G) domain
4	View Details	[294..336] [517..638]	PSI-BLAST	1110.0	Elongation factor eEF-1alpha, domain 2; Elongation factor eEF-1alpha, C-terminal domain; Elongation factor eEF-1alpha, N-terminal (G) domain
5	View Details	[639..747]	PSI-BLAST	1110.0	Elongation factor eEF-1alpha, domain 2; Elongation factor eEF-1alpha, C-terminal domain; Elongation factor eEF-1alpha, N-terminal (G) domain

Functions predicted (by domain):

Gene Ontology predictions

No functions predicted.

Term	Confidence	Notes
ribonuclease activity	4.3040603813219	bayes_pls_golite062009
hydrolase activity	2.55157966585974	bayes_pls_golite062009
exonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 5'-phosphomonoesters	1.44266227866728	bayes_pls_golite062009
binding	1.41264401383678	bayes_pls_golite062009
exonuclease activity	1.15670533472751	bayes_pls_golite062009
nuclease activity	1.06224744208356	bayes_pls_golite062009
catalytic activity	1.00104173493322	bayes_pls_golite062009
structural constituent of ribosome	0.860102064846772	bayes_pls_golite062009
hydrolase activity, acting on ester bonds	0.759324158861044	bayes_pls_golite062009
RNA binding	0.707596564331465	bayes_pls_golite062009
nucleic acid binding	0.625617128641089	bayes_pls_golite062009
purine nucleotide binding	0.569910969126728	bayes_pls_golite062009
purine ribonucleotide binding	0.568840867400671	bayes_pls_golite062009
ribonucleotide binding	0.568779924702229	bayes_pls_golite062009
nucleotide binding	0.564275755355315	bayes_pls_golite062009
	0.40076293158639	bayes_pls_golite062009
translation regulator activity	0.29096092258568	bayes_pls_golite062009
translation factor activity, nucleic acid binding	0.274994961137851	bayes_pls_golite062009
translation termination factor activity	0.269303802954244	bayes_pls_golite062009
translation release factor activity	0.252222008028593	bayes_pls_golite062009
ATP binding	0.1860481660648	bayes_pls_golite062009
adenyl ribonucleotide binding	0.152859582615242	bayes_pls_golite062009
nucleoside-triphosphatase activity	0.147579541298736	bayes_pls_golite062009
adenyl nucleotide binding	0.138615225841109	bayes_pls_golite062009
pyrophosphatase activity	0.133017653323467	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides	0.113504049290503	bayes_pls_golite062009
structural molecule activity	0.11239762372728	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	0.111293884322157	bayes_pls_golite062009
DNA binding	0.0064778111446141	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2