Protein Overview: YRA2

General Information:
Name(s) found:	YRA2 / YKL214C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6322635\|ref\|NP_012708.1\| Member of the REF (RNA and export factor binding proteins) family; when overexpressed, can substitute for the function of Yra1p in export of poly(A)+ mRNA from the nucleus; Yra2p [Saccharomyces cerevisiae] [NCBI NR] gi\|549753\|sp\|P36036.1\|YKV4_YEAST RecName: Full=Uncharacterized RNA-binding protein YKL214C [NCBI NR] pir\|\|S38052 hypothetical protein YKL214c - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|486383\|emb\|CAA82059.1\| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR] gi\|473136\|emb\|CAA53559.1\| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR] Uncharacterized RNA-binding protein YKL214C OS=Saccharomyces cerevisiae GN=YKL214C PE=1 SV=1 [Swiss-Prot] YRA2 mRNA export through interaction with Mex67p [mips] Member of the REF (RNA and export factor binding proteins) family; when overexpressed, can substitute for the function of Yra1p in export of poly(A)+ mRNA from the nucleus [SGD] YKL214C, Chr XI from 31083-31694, reverse complement [yeast_orfs2] Chr XI from 31083-31694, reverse complement [yeastorf3.fasta] Chr XI from 31694-31083, reverse complement [yeastorf5.fasta] (P36036) Hypothetical 23.8 kDa protein in URA1-DOA1 intergenic region [4932.FASTACCERV.txt] YRA2 SGDID:S0001697, Chr XI from 31694-31083, reverse complement, Verified ORF [yeastflysequest.fasta] Member of the REF (RNA and export factor binding proteins) family; when overexpressed, can substitute for the function of Yra1p in export of poly(A)+ mRNA from the nucleus [Saccharomyces cerevisiae]gi\|473136\|emb\|CAA53559.1\| unnamed protein product [Sacch [nrdb_11152004_Chlamydomonas] YRA2 SGDID:S000001697, Chr XI from 31694-31083, reverse complement, Verified ORF, "Member of the REF (RNA and export factor binding proteins) family; when overexpressed, can substitute for the function of Yra1p in export of poly(A)+ mRNA from the nucleus" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] Probable RNA-binding protein YKL214C [nr-271106-contam.fasta] unnamed protein product [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Member of the REF (RNA and export factor binding proteins) family; when overexpressed, can substitute for the function of Yra1p in export of poly(A)+ mRNA from the nucleus; Yra2p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] [chemostatdb.fasta] YRA2 SGDID:S000001697, Chr XI from 31693-31082, Genome Release 64-1-1, reverse complement, Verified ORF, "Member of the REF (RNA and export factor binding proteins) family; when overexpressed, can substitute for the function of Yra1p in export of poly(A)+ mRNA from the nucleus" [yeast-030211-contam.fasta] YRA2 SGDID:S000001697, Chr XI from 31693-31082, Genome Release 64-2-1, reverse complement, Verified ORF, "Member of the REF (RNA and export factor binding proteins) family; when overexpressed, can substitute for the function of Yra1p in export of poly(A)+ mRNA from the nucleus" [20141208_orf_trans.fasta] TR10\|c0_g1_i1\|g.2 ORF TR10\|c0_g1_i1\|g.2 TR10\|c0_g1_i1\|m.2 type:complete len:204 (+) TR10\|c0_g1_i1:66-677(+) [Y12_S_cerevisiae-Sep2015-contam.fasta] YKL214C SGDID:S000001697, Chr XI from 31693-31082, Genome Release 64-1-1, reverse complement, Verified ORF, "Member of the REF (RNA and export factor binding proteins) family; when overexpressed, can substitute for the function of Yra1p in export of poly(A)+ mRNA from the nucleus" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta] asmbl_6569\|g.5046 ORF asmbl_6569\|g.5046 asmbl_6569\|m.5046 type:complete len:204 (+) asmbl_6569:75-686(+) [Y12_Trinity_v2_2_0_PASA_6May2016-contam.fasta] ID=asmbl_6592\|g.4236 ORF ID=asmbl_6592\|g.4236 ID=asmbl_6592\|m.4236 type:complete len:204 (+) ID=asmbl_6592:75-686(+) [Y12_quiver_Trinity_v2_2_0_PASA_2June2016-contam.fasta]
Organism:	Saccharomyces cerevisiae
Length:	203 amino acids
Gene Ontology:
Cellular Component:	nucleus [IDA]
Biological Process:	poly(A)+ mRNA export from nucleus [IGI]
Molecular Function:	RNA binding [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MDKAFDEIIG NSHTDSSSNH KVTRYRRRDL RNELGPRLGF APSDAASRSK DRLYREREEP  60
   61 PLPKRIRISK IPLDVSDYTL DDMIKEFGSP IFSKIFDNKE DRTCIYEFED PEVLEKIVER 120
  121 YNGHELHNAK IEVEIYQPQR KHSRMNAHNR RKQTAQEHGR GRPGSHYRQK PNRVSKKNKG 180
  181 REKNNTPTSV EALDAELDAY MKG

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		CBC2 SRP1 STO1 YRA2
View Details	Krogan NJ, et al. (2006)		CBC2 KAP95 NUP1 NUP2 NUP60 OGG1 PCT1 RAD53 SRP1 STO1 ULP1 URB2 YRA2
View Details	Gavin AC, et al. (2002)		AIR2 BRR1 BUR2 CBC2 HTA1 KAP95 LUC7 MSH4 MUD1 NAB3 NAB6 NAM8 NPL3 NRD1 NUP60 PRP39 PRP40 PRP42 RSE1 SCP160 SEN1 SGV1 SMD1 SMD2 SMD3 SNP1 SNU56 SNU71 SRO9 SRP1 STO1 THO2 TIF4631 TIF4632 YRA2
View Details	Ho Y, et al. (2002)		AAC3 ARP4 ASA1 ATG17 ATG29 ATP3 CDC33 CIC1 CIS1 CYS4 FOL2 GRH1 HAP2 HAP5 IPP1 LOC1 MAM33 MTQ1 NAP1 NMD5 NOP16 NSA2 POL5 PSE1 RHR2 SAH1 SAP190 SPE3 SSK2 TIF1, TIF2 TIF6 YRA1 YRA2 YTM1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	MLP2	#12 Mitotic Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	HMT1	YRA2	1	Unpublished Fields Lab Data
View Screen	DMC1	YRA2	1	Unpublished Fields Lab Data
View Screen	YIF1	YRA2	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	YRA2	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..203]	PSI-BLAST	35.09691	Poly(A)-binding protein

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
nucleic acid binding	3.30589046985932	bayes_pls_golite062009
binding	3.05116833253954	bayes_pls_golite062009
RNA binding	2.47430823231895	bayes_pls_golite062009
protein binding	1.52056358240529	bayes_pls_golite062009
mRNA binding	1.30169099150772	bayes_pls_golite062009
DNA binding	0.202814601806347	bayes_pls_golite062009
poly-purine tract binding	0.15653271757169	bayes_pls_golite062009
transcription regulator activity	0.0111161191366809	bayes_pls_golite062009

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2