Protein Overview: TRP3

General Information:
Name(s) found:	TRP3 / YKL211C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6322638\|ref\|NP_012711.1\| Bifunctional enzyme exhibiting both indole-3-glycerol-phosphate synthase and anthranilate synthase activities, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex wi [NCBI NR] gi\|549127\|sp\|P00937.2\|TRPG_YEAST RecName: Full=Anthranilate synthase component 2; AltName: Full=Anthranilate synthase component II; Includes: RecName: Full=Glutamine amidotransferase; Includes: RecName: Full=Indole-3-glycerol phosphate synthase; AltName: [NCBI NR] pir\|\|NNBY2 anthranilate synthase multifunctional enzyme - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|486377\|emb\|CAA82056.1\| TRP3 [Saccharomyces cerevisiae] [NCBI NR] gi\|473139\|emb\|CAA53562.1\| TRP3 [Saccharomyces cerevisiae] [NCBI NR] Anthranilate synthase component 2 OS=Saccharomyces cerevisiae GN=TRP3 PE=1 SV=2 [Swiss-Prot] TRP3 Anthranilate synthase component II [mips] Bifunctional enzyme exhibiting both indole-3-glycerol-phosphate synthase and anthranilate synthase activities, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p [SGD] TRP3, Chr XI from 36700-38154, reverse complement [yeast_orfs2] Chr XI from 36700-38154, reverse complement [yeastorf3.fasta] Chr XI from 38154-36700, reverse complement [yeastorf5.fasta] (P00937) Anthranilate synthase component II (EC 4.1.3.27) [Includes: Glutamine amidotransferase; Indole-3-glycerol phosphate synthase (EC 4.1.1.48) (PRAI)] [4932.FASTACCERV.txt] TRP3 SGDID:S0001694, Chr XI from 38154-36700, reverse complement, Verified ORF [yeastflysequest.fasta] Trp3p [Saccharomyces cerevisiae]gi\|473139\|emb\|CAA53562.1\| TRP3 [Saccharomyces cerevisiae]gi\|486377\|emb\|CAA82056.1\| TRP3 [Saccharomyces cerevisiae]gi\|538557\|pir\|\|NNBY2 anthranilate synthase multifunctional enzyme - yeast (Saccharomyces cerevisiae)gi\|5 [nrdb_11152004_Chlamydomonas] TRP3 SGDID:S000001694, Chr XI from 38154-36700, reverse complement, Verified ORF, "Bifunctional enzyme exhibiting both indole-3-glycerol-phosphate synthase and anthranilate synthase activities, forms multifunctional hetero-oligomeric anthranilate synthase [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] TRP3 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Bifunctional enzyme exhibiting both indole-3-glycerol-phosphate synthase and anthranilate synthase activities, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p; Trp3p [Saccharomyc [nr-271106-contam.fasta] Anthranilate synthase component 2 (Anthranilate synthase component II) [Includes: Glutamine amidotransferase; Indole-3-glycerol phosphate synthase (PRAI)] [nr-271106-contam.fasta] TRP3 SGDID:S000001694, Chr XI from 38154-36700, reverse complement, Verified ORF, "Bifunctional enzyme exhibiting both indole-3-glycerol-phosphate synthase and anthranilate synthase activities, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p" [yeast-bovrabprots.fasta] [chemostatdb.fasta] Anthranilate synthase component 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=TRP3 PE=1 SV=2 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] TRP3 SGDID:S000001694, Chr XI from 38153-36699, Genome Release 64-1-1, reverse complement, Verified ORF, "Bifunctional enzyme exhibiting both indole-3-glycerol-phosphate synthase and anthranilate synthase activities, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p" [yeast-030211-contam.fasta] TRP3 SGDID:S000001694, Chr XI from 38153-36699, Genome Release 64-2-1, reverse complement, Verified ORF, "Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p" [20141208_orf_trans.fasta] Multifunctional tryptophan biosynthesis protein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=TRP3 PE=1 SV=2 [ACHE_BCHE_APH_CES_allEnz_HUMAN.fasta] TR1905\|c0_g1_i1\|g.1377 ORF TR1905\|c0_g1_i1\|g.1377 TR1905\|c0_g1_i1\|m.1377 type:complete len:485 (+) TR1905\|c0_g1_i1:79-1533(+) [Y12_S_cerevisiae-Sep2015-contam.fasta] TR1905\|c0_g2_i1\|g.1378 ORF TR1905\|c0_g2_i1\|g.1378 TR1905\|c0_g2_i1\|m.1378 type:complete len:485 (+) TR1905\|c0_g2_i1:79-1533(+) [Y12_S_cerevisiae-Sep2015-contam.fasta] TR1905\|c0_g3_i1\|g.1379 ORF TR1905\|c0_g3_i1\|g.1379 TR1905\|c0_g3_i1\|m.1379 type:complete len:485 (+) TR1905\|c0_g3_i1:79-1533(+) [Y12_S_cerevisiae-Sep2015-contam.fasta] TR1905\|c0_g4_i1\|g.1380 ORF TR1905\|c0_g4_i1\|g.1380 TR1905\|c0_g4_i1\|m.1380 type:complete len:485 (+) TR1905\|c0_g4_i1:79-1533(+) [Y12_S_cerevisiae-Sep2015-contam.fasta] YKL211C SGDID:S000001694, Chr XI from 38153-36699, Genome Release 64-1-1, reverse complement, Verified ORF, "Bifunctional enzyme exhibiting both indole-3-glycerol-phosphate synthase and anthranilate synthase activities, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta] asmbl_6567\|g.4871 ORF asmbl_6567\|g.4871 asmbl_6567\|m.4871 type:complete len:485 (+) asmbl_6567:78-1532(+) [Y12_Trinity_v2_2_0_PASA_6May2016-contam.fasta] ID=asmbl_6590\|g.4233 ORF ID=asmbl_6590\|g.4233 ID=asmbl_6590\|m.4233 type:complete len:485 (+) ID=asmbl_6590:78-1532(+) [Y12_quiver_Trinity_v2_2_0_PASA_2June2016-contam.fasta]
Organism:	Saccharomyces cerevisiae
Length:	484 amino acids
Gene Ontology:
Cellular Component:	cytoplasm [IDA] anthranilate synthase complex [IPI]
Biological Process:	tryptophan biosynthetic process [TAS]
Molecular Function:	indole-3-glycerol-phosphate synthase activity [IDA][IGI][ISS] anthranilate synthase activity [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSVHAATNPI NKHVVLIDNY DSFTWNVYEY LCQEGAKVSV YRNDAITVPE IAALNPDTLL  60
   61 ISPGPGHPKT DSGISRDCIR YFTGKIPVFG ICMGQQCMFD VFGGEVAYAG EIVHGKTSPI 120
  121 SHDNCGIFKN VPQGIAVTRY HSLAGTESSL PSCLKVTAST ENGIIMGVRH KKYTVEGVQF 180
  181 HPESILTEEG HLMIRNILNV SGGTWEENKS SPSNSILDRI YARRKIDVNE QSKIPGFTFQ 240
  241 DLQSNYDLGL APPLQDFYTV LSSSHKRAVV LAEVKRASPS KGPICLKAVA AEQALKYAEA 300
  301 GASAISVLTE PHWFHGSLQD LVNVRKILDL KFPPKERPCV LRKEFIFSKY QILEARLAGA 360
  361 DTVLLIVKML SQPLLKELYS YSKDLNMEPL VEVNSKEELQ RALEIGAKVV GVNNRDLHSF 420
  421 NVDLNTTSNL VESIPKDVLL IALSGITTRD DAEKYKKEGV HGFLVGEALM KSTDVKKFIH 480
  481 ELCE

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		TRP2 TRP3
View Details	(MIPS) Mewes HW, et al. (2004)		TRP2 TRP3
View Details	Krogan NJ, et al. (2006)		STB3 TRP2 TRP3
View Details	Gavin AC, et al. (2006)		SAM1 TRP2 TRP3
View Details	Gavin AC, et al. (2002)		SAM1 TRP2 TRP3
View Details	Ho Y, et al. (2002)		ACC1 ADR1 ARC15 ARC18 ARC19 ARC35 ARC40 ARP2 ARP3 ATP3 BGL2 BNI1 CBP6 COP1 DMC1 DPM1 DUR1,2 FRS2 GCD11 GCN3 HXT7 IMH1 KAP122 MCM4 MCM7 MCX1 MDJ1 MET18 NDE1 NOP14 NPA3 PET10 PET9 PHO86 PRB1 RET2 RPT4 RVB2 SEC21 SEC26 SEC27 SEC28 SLC1 TRP3 TRP5 VPS8 YJR029W
View Details	Qiu et al. (2008)		TRP2 TRP3

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	RPA135	his-HA tag on RPA135	Schneider, DA, et al. (2006)
View Run	MLP2	#32 Asynchronous Prep-No Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#31 Asynchronous Prep (Protease cleavage)	Keck JM, et al. (2011)
View Run	MLP2	#29 Asynchronous Prep5-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	None Entered	#17 Mitotic Prep3-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	CTF13	TRP3	1	Unpublished Fields Lab Data
View Screen	RAD1	TRP3	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	TRP3	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..252]	MSA	299.454997	View MSA. No confident structure predictions are available.
2	View Details	[253..484]	PSI-BLAST	387.201249	Indole-3-glycerophosphate synthase, IPGS

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
anthranilate synthase activity	9.07528926783927	bayes_pls_golite062009
oxo-acid-lyase activity	7.16633518631507	bayes_pls_golite062009
4-amino-4-deoxychorismate synthase activity	4.48164623771604	bayes_pls_golite062009
indole-3-glycerol-phosphate synthase activity	4.14261580175621	bayes_pls_golite062009
ligase activity	1.70348758031146	bayes_pls_golite062009
catalytic activity	1.30391542469987	bayes_pls_golite062009
nucleic acid binding	0.86323855032533	bayes_pls_golite062009
ligase activity, forming carbon-nitrogen bonds	0.628648036096965	bayes_pls_golite062009
binding	0.588714599255874	bayes_pls_golite062009
imidazoleglycerol-phosphate synthase activity	0.270391651600004	bayes_pls_golite062009
carbon-carbon lyase activity	0.0769356405014503	bayes_pls_golite062009
protein binding	0.0152024556625711	bayes_pls_golite062009

Term	Confidence	Notes
indole-3-glycerol-phosphate synthase activity	8.59736519932798	bayes_pls_golite062009
anthranilate synthase activity	5.47492192345361	bayes_pls_golite062009
oxo-acid-lyase activity	4.09809510052304	bayes_pls_golite062009
tryptophan synthase activity	2.99898167480072	bayes_pls_golite062009
phosphoribosylanthranilate isomerase activity	2.57188946638955	bayes_pls_golite062009
catalytic activity	2.44231061702877	bayes_pls_golite062009
carbon-carbon lyase activity	1.80077901589281	bayes_pls_golite062009
carboxy-lyase activity	1.32677036501434	bayes_pls_golite062009
ribulose-phosphate 3-epimerase activity	0.966175242934785	bayes_pls_golite062009
binding	0.626392473915547	bayes_pls_golite062009
imidazoleglycerol-phosphate synthase activity	0.567370625225674	bayes_pls_golite062009
lyase activity	0.258652421339738	bayes_pls_golite062009
protein binding	0.014429239696785	bayes_pls_golite062009

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2