Protein Overview: BFA1

General Information:
Name(s) found:	BFA1 / YJR053W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6322513\|ref\|NP_012587.1\| Component of the GTPase-activating Bfa1p-Bub2p complex involved in multiple cell cycle checkpoint pathways that control exit from mitosis; Bfa1p [Saccharomyces cerevisiae] [NCBI NR] gi\|1352883\|sp\|P47113.1\|BFA1_YEAST RecName: Full=Mitotic check point protein BFA1; AltName: Full=Cell cycle arrest protein BFA1 [NCBI NR] gi\|1197082\|gb\|AAA88756.1\| ORF; putative [Saccharomyces cerevisiae] [NCBI NR] pir\|\|S57072 hypothetical protein YJR053w - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|1015717\|emb\|CAA89581.1\| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR] Mitotic check point protein BFA1 OS=Saccharomyces cerevisiae GN=BFA1 PE=1 SV=1 [Swiss-Prot] BFA1 Spindle assembly checkpoint protein [mips] Component of the GTPase-activating Bfa1p-Bub2p complex involved in multiple cell cycle checkpoint pathways that control exit from mitosis [SGD] BFA1, Chr X from 533719-535443 [yeast_orfs2] Chr X from 533719-535443 [yeastorf3.fasta] Chr X from 533720-535444 [yeastorf4.fasta] Chr X from 533721-535445 [yeastorf5.fasta] (P47113) Mitotic check point protein BFA1 (Cell cycle arrest protein BFA1) [4932.FASTACCERV.txt] BFA1 SGDID:S0003814, Chr X from 533941-535665, Verified ORF [yeastflysequest.fasta] Bfa1p [Saccharomyces cerevisiae]gi\|1015717\|emb\|CAA89581.1\| unnamed protein product [Saccharomyces cerevisiae]gi\|1077883\|pir\|\|S57072 hypothetical protein YJR053w - yeast (Saccharomyces cerevisiae)gi\|1197082\|gb\|AAA88756.1\| ORF; putativegi\|1352883\|sp\|P47 [nrdb_11152004_Chlamydomonas] BFA1 SGDID:S000003814, Chr X from 533941-535665, Verified ORF, "Component of the GTPase-activating Bfa1p-Bub2p complex involved in multiple cell cycle checkpoint pathways that control exit from mitosis" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] BFA1 SGDID:S000003814, Chr X from 534020-535744, Verified ORF, "Component of the GTPase-activating Bfa1p-Bub2p complex involved in multiple cell cycle checkpoint pathways that control exit from mitosis" [yeast-contam.fasta] BFA1 SGDID:S000003814, Chr X from 534019-535743, Verified ORF, "Component of the GTPase-activating Bfa1p-Bub2p complex involved in multiple cell cycle checkpoint pathways that control exit from mitosis" [yeast-200209-contam.fasta] ORF; putative [nr-271106-contam.fasta] Mitotic check point protein BFA1 (Cell cycle arrest protein BFA1) [nr-271106-contam.fasta] unnamed protein product [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Component of the GTPase-activating Bfa1p-Bub2p complex involved in multiple cell cycle checkpoint pathways that control exit from mitosis; Bfa1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] [chemostatdb.fasta] Mitotic check point protein BFA1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=BFA1 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] BFA1 SGDID:S000003814, Chr X from 534027-535751, Genome Release 64-1-1, Verified ORF, "Component of the GTPase-activating Bfa1p-Bub2p complex involved in multiple cell cycle checkpoint pathways that control exit from mitosis" [yeast-030211-contam.fasta] BFA1 SGDID:S000003814, Chr X from 534027-535751, Genome Release 64-2-1, Verified ORF, "Component of the GTPase-activating Bfa1p-Bub2p complex; involved in multiple cell cycle checkpoint pathways that control exit from mitosis; specifically required when telomeres are damaged, but not for all types of chromosomal DNA damage; phosphorylated by the Polo-like kinase Cdc5p" [20141208_orf_trans.fasta] YJR053W SGDID:S000003814, Chr X from 534027-535751, Genome Release 64-1-1, Verified ORF, "Component of the GTPase-activating Bfa1p-Bub2p complex involved in multiple cell cycle checkpoint pathways that control exit from mitosis" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	574 amino acids
Gene Ontology:
Cellular Component:	spindle pole body [IDA]
Biological Process:	regulation of exit from mitosis [TAS] mitotic cell cycle spindle orientation checkpoint [IGI][IMP]
Molecular Function:	GTPase activator activity [TAS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSIRPLTLNG LDEPETSFEE LNTTLPRFQS HETLTLEENV PPLSTSTYIP PPSSVGTSDT  60
   61 GTVFSNSTSA FWSNKQADDD QDMEVDQDDE FLNDFQEFQN KKDDFDDAIK TNFHLRNGCR 120
  121 TGPFKNDIFA EEFDRKLSLE DKPRLKQPRS MMELKPKRKL SNSVTSRNLR SGNSVRFKKS 180
  181 MPNLALVNPA IREEEEDEER EREDQREFNY KIDNDTQDTI LAKFSSDDEG DFLTGFEELE 240
  241 GEAIDETISS NDKESADHPR FLKKSSSSLP LKISPAQYDI VKHDELLTPG LHRRQRDWNT 300
  301 QQELDSFKEK RSVRHCSNQN VQLNGPAKIK TIKQQIDHNT PMKKGSMIYN PKTMKWEGNE 360
  361 NVLSKFSDVD TANRKALLIK NKLQRDADSK KQKYSDLQHA RATSRNQKVI GNMILDEQNL 420
  421 RWVSVSEEEA DPFAGIPEIN LPPVGKSMKK RSSSPFLRSK SQVNTPFVSN DNDGVYQSTA 480
  481 AQARLRKYHS MRTLNGTTET PEISSTFHLS SRALEKFYHE ENRWCKKLAS WFIPRDETII 540
  541 SVDEETIMDE STVNSKRKSY MYEIRNMVIN STKD

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Krogan NJ, et al. (2006)		BFA1 CDC48 DOA1 FBP1 MRM1 NDT80 NPL4 OTU1 RDS2 SHP1 TEM1 TUP1 UBX4 UBX5 UBX7 UFD1 UFD2 YDR049W YGL108C YJR098C YNL155W YOR342C
View Details	Ho Y, et al. (2002)		BFA1 CDC15 CLU1 DUG2 FAA4 FET4 GPG1 HAS1 KEX2 NUP53 RNR1 RNR2 RVB1 SSD1 STE23 TEM1 YTM1
View Details	Qiu et al. (2008)		BFA1 BUB2 GIC1 MOB1 SGO1 STU1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	MLP2	#33 Asynchronous SPB prep	Keck JM, et al. (2011)
View Run	MLP2	#19 Asynchronous Prep1-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#25 Asynchronous Prep3-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#24 Asynchronous Prep3-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#26 Asynchronous Prep4-TiO2 Phosphopeptide enriched, Steps1-2	Keck JM, et al. (2011)
View Run	MLP2	#27 Asynchronous Prep4-TiO2 Phosphopeptide enriched, Steps3-5	Keck JM, et al. (2011)
View Run	MLP2	#21 Asynchronous Prep2-IMAC Phosphopeptide enrichment A1	Keck JM, et al. (2011)
View Run	MLP2	#22 Asynchronous Prep2-IMAC Phosphopeptide enrichment B1-2	Keck JM, et al. (2011)
View Run	MLP2	#34 Asynchronous SPB prep	Keck JM, et al. (2011)
View Run	MLP2	#32 Asynchronous Prep-No Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#35 Asynchronous SPB prep	Keck JM, et al. (2011)
View Run	MLP2	#31 Asynchronous Prep (Protease cleavage)	Keck JM, et al. (2011)
View Run	MLP2	#03 Alpha Factor Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#29 Asynchronous Prep5-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#14 Mitotic Prep2-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#13 Mitotic Prep2-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#12 Mitotic Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#10 Mitotic Prep1-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#02 Alpha Factor Prep1-TiO2 enriched, new search criteria	Keck JM, et al. (2011)
View Run	MLP2	#30 Asynchronous Prep6-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#11 Mitotic Prep1-TiO2 enriched, new search criteria	Keck JM, et al. (2011)
View Run	MLP2	#01 Alpha factor Prep1-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#28 Asynchronous Prep5-TiO2 Phosphopeptide Enrichment	Keck JM, et al. (2011)
View Run	MLP2	#06 Alpha Factor Prep3-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#08 Alpha Factor Prep4-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#16 Mitotic-New search criteria	Keck JM, et al. (2011)
View Run	MLP2	#15 Mitotic 2nd-TiO2 enriched, new search criteria	Keck JM, et al. (2011)
View Run	MLP2	#19b Asynchronous Prep1-new search criteria	Keck JM, et al. (2011)
View Run	MLP2	#05 Alpha Factor Prep2-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#07 Alpha Factor Prep3-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	None Entered	#17 Mitotic Prep3-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	None Entered	#18 Mitotic Prep4-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#19a Asynchronous Prep1-new search criteria	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

No yeast two-hybrid interactions found for this protein.

	PROTEIN(S)	PUBLICATION
View Data	BFA1	Huh WK, et al. (2003)
View Data	BFA1 and SPC42	Huh WK, et al. (2003)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..325]	deduced	N/A	No confident structure predictions are available.
2	View Details	[326..440]	MSA	1.003959	View MSA. No confident structure predictions are available.
3	View Details	[441..574]	deduced	N/A	No confident structure predictions are available.
4	View Details	[440..574]	deduced	N/A	No confident structure predictions are available.

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Protein Overview: BFA1

Protein Complex Data

Mass Spectrometry Data

Yeast Two-Hybrid Data

Microscopy / Localization Data

Protein Structure Data

Philius Transmembrane Prediction: