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Protein Overview: ISY1

General Information:
Name(s) found: ISY1 / YJR050W [SGD]
Description(s) found:
Found 35 descriptions.
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  • Member of NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of spliceosome containing U2, U5, and U6 snRNAs, interacts with Prp16p to modulate splicing fidelity; isy1 syf2 cells have defective spindles [SGD]
    • Organism: Saccharomyces cerevisiae
    Length: 235 amino acids

    Gene Ontology:
    Cellular Component: spliceosomal complex [IDALink to PubMed article][IGILink to PubMed article]
    Biological Process: nuclear mRNA splicing, via spliceosome [IDALink to PubMed article][IGILink to PubMed article]
    Molecular Function: second spliceosomal transesterification activity [IC]
    first spliceosomal transesterification activity [IGI]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSRNVDKANS VLVRFQEQQA ESAGGYKDYS RYQRPRNVSK VKSIKEANEW KRQVSKEIKQ  60
   61 KSTRIYDPSL NEMQIAELND ELNNLFKEWK RWQWHIDHTL MEKKTKRKRL EDSHVLMNSG 120
  121 KLINGKRYFG RALELPEVKE WLKQSQRQND GGSINTKCIP KDRNDFYYHG KVTAALTEFE 180
  181 ANWTSILKAH YNVPVNEDEE EMSRQTQEIH VPTLADMEHW LVQRRKKKLM DELNL

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details Riffle et al. (2010) (Unpublished Data) CEF1 CLF1 ISY1 PRP19 PRP45 PRP46 SNT309 SYF1 SYF2
    View Details Hazbun TR, et al. (2003) BRR2 CDC40 CEF1 CLF1 CWC2 CWC22 CWC23 ECM2 ISY1 LEA1 NTR2 PRP19 PRP43 PRP45 PRP46 PRP8 SMB1 SMD1 SMD3 SNT309 SNU114 SPP382 SYF1 SYF2 YJU2
    View Details Krogan NJ, et al. (2006) CEF1 CLF1 ISY1 NTC20 SEC6 SNT309 SYF1 SYF2
    View Details Gavin AC, et al. (2002) BUD31 CDC40 CEF1 CLF1 CWC2 CWC22 CWC23 ECM2 HSH155 ISY1 LEA1 PRP19 PRP2 PRP21 PRP22 PRP43 PRP45 PRP46 RSE1 SMD2 SNT309 SNU114 SPP382 STO1 SYF1
    View Details Qiu et al. (2008) AAR2 BRR1 BRR2 CBC2 CDC40 CEF1 CLF1 CUS1 CUS2 CWC15 CWC2 CWC22 CWC23 DHR2 DIB1 ECM2 HSH155 IST3 ISY1 LEA1 LSM2 LSM3 LSM4 LSM7 LSM8 MAK5 MUD1 MUD2 NAM8 NTC20 PRP11 PRP16 PRP18 PRP19 PRP2 PRP21 PRP22 PRP24 PRP28 PRP3 PRP31 PRP38 PRP39 PRP4 PRP40 PRP42 PRP43 PRP45 PRP46 PRP5 PRP6 PRP8 PRP9 RSE1 SLU7 SMB1 SMD1 SMD2 SMD3 SME1 SMX2 SMX3 SNP1 SNT309 SNU114 SNU23 SNU56 SNU66 SNU71 SPP2 SPP381 SPP382 STO1 SYF1 SYF2 YHC1

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run SPP382 No Comments Hazbun TR, et al. (2003)
    View Run CWC25 No Comments Hazbun TR, et al. (2003)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    SHOWING SINGLE HITS. [ Hide Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

      BAIT PREY HITS PUBLICATION
    View Screen SEC17 ISY1 1 Unpublished Fields Lab Data

    Microscopy / Localization Data

    New Feature: Upload Your Own Microscopy Data

      PROTEIN(S) PUBLICATION
    View Data ISY1 Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..104] Pcons 1.55 Spectrin
    2 View Details [105..235] Pcons 1.55 Spectrin

    Functions predicted (by domain):

    # Gene Ontology predictions
    1
    Term Confidence Notes
  • binding
    		•  2.7761451438984 bayes_pls_golite062009
  • cytoskeletal protein binding
    		•  2.28760768499625 bayes_pls_golite062009
  • structural molecule activity
    		•  2.18199613481266 bayes_pls_golite062009
  • actin binding
    		•  2.06460396235438 bayes_pls_golite062009
  • protein binding
    		•  2.03402581943916 bayes_pls_golite062009
  • actin filament binding
    		•  1.58451318432641 bayes_pls_golite062009
  • transcription regulator activity
    		•  1.33758861760616 bayes_pls_golite062009
  • protein complex binding
    		•  0.704042118621314 bayes_pls_golite062009
  • identical protein binding
    		•  0.123910502598633 bayes_pls_golite062009
    2
    Term Confidence Notes
  • binding
    		•  2.7761451438984 bayes_pls_golite062009
  • cytoskeletal protein binding
    		•  2.28760768499625 bayes_pls_golite062009
  • structural molecule activity
    		•  2.18199613481266 bayes_pls_golite062009
  • actin binding
    		•  2.06460396235438 bayes_pls_golite062009
  • protein binding
    		•  2.03402581943916 bayes_pls_golite062009
  • actin filament binding
    		•  1.58451318432641 bayes_pls_golite062009
  • transcription regulator activity
    		•  1.33758861760616 bayes_pls_golite062009
  • protein complex binding
    		•  0.704042118621314 bayes_pls_golite062009
  • identical protein binding
    		•  0.123910502598633 bayes_pls_golite062009


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.99

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle