Protein Overview: BUB1

General Information:
Name(s) found:	BUB1 / YGR188C [SGD]
Description(s) found: Found 28 descriptions. SHOW ALL	Protein kinase that forms a complex with Mad1p and Bub3p that is crucial in the checkpoint mechanism required to prevent cell cycle progression into anaphase in the presence of spindle damage, associates with centromere DNA via Skp1p [SGD]
Organism:	Saccharomyces cerevisiae
Length:	1021 amino acids
Gene Ontology:
Cellular Component:	nucleus [IDA] condensed nuclear chromosome, centromeric region [IDA] condensed nuclear chromosome kinetochore [IDA] kinetochore [IDA]
Biological Process:	mitotic cell cycle spindle assembly checkpoint [TAS] protein amino acid phosphorylation [IDA]
Molecular Function:	protein binding [IPI] protein kinase activity [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MNLDLGSTVR GYESDKDTFP QSKGVSSSQK EQHSQLNQTK IAYEQRLLND LEDMDDPLDL  60
   61 FLDYMIWIST SYIEVDSESG QEVLRSTMER CLIYIQDMET YRNDPRFLKI WIWYINLFLS 120
  121 NNFHESENTF KYMFNKGIGT KLSLFYEEFS KLLENAQFFL EAKVLLELGA ENNCRPYNRL 180
  181 LRSLSNYEDR LREMNIVENQ NSVPDSRERL KGRLIYRTAP FFIRKFLTSS LMTDDKENRA 240
  241 NLNSNVGVGK SAPNVYQDSI VVADFKSETE RLNLNSSKQP SNQRLKNGNK KTSIYADQKQ 300
  301 SNNPVYKLIN TPGRKPERIV FNFNLIYPEN DEEFNTEEIL AMIKGLYKVQ RRGKKHTEDY 360
  361 TSDKNRKKRK LDVLVERRQD LPSSQPPVVP KSTRIEVFKD DDNPSQSTHH KNTQVQVQTT 420
  421 TSILPLKPVV DGNLAHETPV KPSLTSNASR SPTVTAFSKD AINEVFSMFN QHYSTPGALL 480
  481 DGDDTTTSKF NVFENFTQEF TAKNIEDLTE VKDPKQETVS QQTTSTNETN DRYERLSNSS 540
  541 TRPEKADYMT PIKETTETDV VPIIQTPKEQ IRTEDKKSGD NTETQTQLTS TTIQSSPFLT 600
  601 QPEPQAEKLL QTAEHSEKSK EHYPTIIPPF TKIKNQPPVI IENPLSNNLR AKFLSEISPP 660
  661 LFQYNTFYNY NQELKMSSLL KKIHRVSRNE NKNPIVDFKK TGDLYCIRGE LGEGGYATVY 720
  721 LAESSQGHLR ALKVEKPASV WEYYIMSQVE FRLRKSTILK SIINASALHL FLDESYLVLN 780
  781 YASQGTVLDL INLQREKAID GNGIMDEYLC MFITVELMKV LEKIHEVGII HGDLKPDNCM 840
  841 IRLEKPGEPL GAHYMRNGED GWENKGIYLI DFGRSFDMTL LPPGTKFKSN WKADQQDCWE 900
  901 MRAGKPWSYE ADYYGLAGVI HSMLFGKFIE TIQLQNGRCK LKNPFKRYWK KEIWGVIFDL 960
  961 LLNSGQASNQ ALPMTEKIVE IRNLIESHLE QHAENHLRNV ILSIEEELSH FQYKGKPSRR1020
 1021 F

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Krogan NJ, et al. (2006)		ATG33 BUB1 CUL3 GUT1 ISM1 MEC3 MLC1 MYO2 MYO4 RGA1 RSB1 SAT4 SHE2 SHE3 SHE4 SRC1 SSH4 STT4 TOF2 TVP18 YDR186C YOL098C
View Details	Qiu et al. (2008)		BIK1 BUB1 BUB3 HSK3 SLK19 STU1 TID3

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	BOI1	Sample boi 1 with ha tag from october 2005	McCusker D, et al (2007)
View Run	MLP2	#26 Asynchronous Prep4-TiO2 Phosphopeptide enriched, Steps1-2	Keck JM, et al. (2011)
View Run	MLP2	#32 Asynchronous Prep-No Phosphopeptide enrichment	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	SKP1	BUB1	2	Unpublished Fields Lab Data
View Screen	SKP1	BUB1	2	Unpublished Fields Lab Data
View Screen	BUB1	CBS2	2	Unpublished Fields Lab Data
View Screen	BUB1	TOM6	2	Unpublished Fields Lab Data
View Screen	BUB3	BUB1	2	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	BUB1	Huh WK, et al. (2003)
View Data	BUB1 and SPC42	Huh WK, et al. (2003)

Protein Structure Data

[What does the above image mean?]
[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..136]	deduced	N/A	No confident structure predictions are available.
2	View Details	[137..211]	PSI-BLAST	3.69897	Effector domain of the protein kinase pkn/prk1
3	View Details	[212..452]	deduced	N/A	No confident structure predictions are available.
4	View Details	[453..589]	PSI-BLAST	11.0	Photoreceptor phy3 flavin-binding domain, lov2
5	View Details	[590..663]	PSI-BLAST	6.221849	C2 domain from protein kinase c (beta)
6	View Details	[664..691] [751..770] [781..1021]	PSI-BLAST	105.0	cAMP-dependent PK, catalytic subunit
7	View Details	[692..750] [771..780]	PSI-BLAST	105.0	cAMP-dependent PK, catalytic subunit

Functions predicted (by domain):

Gene Ontology predictions

No functions predicted.

Term	Confidence	Notes
transcription regulator activity	3.69172569316336	bayes_pls_golite062009
DNA binding	3.39869859972616	bayes_pls_golite062009
nucleic acid binding	3.23848471176801	bayes_pls_golite062009
binding	3.13193163173466	bayes_pls_golite062009
transcription factor activity	2.84890910865229	bayes_pls_golite062009
transcription factor binding	2.24654471792947	bayes_pls_golite062009
RNA polymerase II transcription factor activity	1.94402505264757	bayes_pls_golite062009
transcription activator activity	1.7419016121428	bayes_pls_golite062009
protein binding	1.73179783495389	bayes_pls_golite062009
kinase activity	1.65716179379575	bayes_pls_golite062009
protein kinase activity	1.65559891124729	bayes_pls_golite062009
sequence-specific DNA binding	1.49417707466137	bayes_pls_golite062009
protein heterodimerization activity	1.3916255846493	bayes_pls_golite062009
transporter activity	1.345463646363	bayes_pls_golite062009
protein dimerization activity	1.3011414258578	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	1.25717484863319	bayes_pls_golite062009
phosphotransferase activity, alcohol group as acceptor	1.23751270866642	bayes_pls_golite062009
voltage-gated cation channel activity	1.2111783925162	bayes_pls_golite062009
transmembrane transporter activity	1.1928367617123	bayes_pls_golite062009
transcription repressor activity	1.16489293306631	bayes_pls_golite062009
substrate-specific transporter activity	1.10368550509667	bayes_pls_golite062009
voltage-gated potassium channel activity	1.09066951584628	bayes_pls_golite062009
voltage-gated ion channel activity	1.0878469536455	bayes_pls_golite062009
voltage-gated channel activity	1.08700038835943	bayes_pls_golite062009
cation channel activity	1.05127986997493	bayes_pls_golite062009
potassium channel activity	1.00381478626553	bayes_pls_golite062009
substrate-specific transmembrane transporter activity	0.9754275279592	bayes_pls_golite062009
cation transmembrane transporter activity	0.97440822227802	bayes_pls_golite062009
transcription cofactor activity	0.97238321935796	bayes_pls_golite062009
gated channel activity	0.96773601513941	bayes_pls_golite062009
ion transmembrane transporter activity	0.94560998710422	bayes_pls_golite062009
ion channel activity	0.913028049156819	bayes_pls_golite062009
substrate-specific channel activity	0.86162931627036	bayes_pls_golite062009
transcription coactivator activity	0.85685043852877	bayes_pls_golite062009
passive transmembrane transporter activity	0.84703861081342	bayes_pls_golite062009
channel activity	0.84703861081342	bayes_pls_golite062009
histone acetyltransferase binding	0.806615474344071	bayes_pls_golite062009
metal ion transmembrane transporter activity	0.7482723372563	bayes_pls_golite062009
RNA polymerase II transcription factor activity, enhancer binding	0.63697280374462	bayes_pls_golite062009
transferase activity	0.511065872472486	bayes_pls_golite062009

No functions predicted.

Term	Confidence	Notes
transcription regulator activity	3.69172569316336	bayes_pls_golite062009
DNA binding	3.39869859972616	bayes_pls_golite062009
nucleic acid binding	3.23848471176801	bayes_pls_golite062009
binding	3.13193163173466	bayes_pls_golite062009
transcription factor activity	2.84890910865229	bayes_pls_golite062009
transcription factor binding	2.24654471792947	bayes_pls_golite062009
RNA polymerase II transcription factor activity	1.94402505264757	bayes_pls_golite062009
transcription activator activity	1.7419016121428	bayes_pls_golite062009
protein binding	1.73179783495389	bayes_pls_golite062009
kinase activity	1.65716179379575	bayes_pls_golite062009
protein kinase activity	1.65559891124729	bayes_pls_golite062009
sequence-specific DNA binding	1.49417707466137	bayes_pls_golite062009
protein heterodimerization activity	1.3916255846493	bayes_pls_golite062009
transporter activity	1.345463646363	bayes_pls_golite062009
protein dimerization activity	1.3011414258578	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	1.25717484863319	bayes_pls_golite062009
phosphotransferase activity, alcohol group as acceptor	1.23751270866642	bayes_pls_golite062009
voltage-gated cation channel activity	1.2111783925162	bayes_pls_golite062009
transmembrane transporter activity	1.1928367617123	bayes_pls_golite062009
transcription repressor activity	1.16489293306631	bayes_pls_golite062009
substrate-specific transporter activity	1.10368550509667	bayes_pls_golite062009
voltage-gated potassium channel activity	1.09066951584628	bayes_pls_golite062009
voltage-gated ion channel activity	1.0878469536455	bayes_pls_golite062009
voltage-gated channel activity	1.08700038835943	bayes_pls_golite062009
cation channel activity	1.05127986997493	bayes_pls_golite062009
potassium channel activity	1.00381478626553	bayes_pls_golite062009
substrate-specific transmembrane transporter activity	0.9754275279592	bayes_pls_golite062009
cation transmembrane transporter activity	0.97440822227802	bayes_pls_golite062009
transcription cofactor activity	0.97238321935796	bayes_pls_golite062009
gated channel activity	0.96773601513941	bayes_pls_golite062009
ion transmembrane transporter activity	0.94560998710422	bayes_pls_golite062009
ion channel activity	0.913028049156819	bayes_pls_golite062009
substrate-specific channel activity	0.86162931627036	bayes_pls_golite062009
transcription coactivator activity	0.85685043852877	bayes_pls_golite062009
passive transmembrane transporter activity	0.84703861081342	bayes_pls_golite062009
channel activity	0.84703861081342	bayes_pls_golite062009
histone acetyltransferase binding	0.806615474344071	bayes_pls_golite062009
metal ion transmembrane transporter activity	0.7482723372563	bayes_pls_golite062009
RNA polymerase II transcription factor activity, enhancer binding	0.63697280374462	bayes_pls_golite062009
transferase activity	0.511065872472486	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.98

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2