Protein Overview: EMP24

General Information:
Name(s) found:	EMP24 / YGL200C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6321238\|ref\|NP_011315.1\| Integral membrane component of endoplasmic reticulum-derived COPII-coated vesicles, which function in ER to Golgi transport; Emp24p [Saccharomyces cerevisiae] [NCBI NR] gi\|417435\|sp\|P32803.1\|EMP24_YEAST RecName: Full=Endosomal protein P24B; AltName: Full=Basic 24 kDa late endocytic intermediate component; AltName: Full=24 kDa endomembrane protein; Flags: Precursor [NCBI NR] gi\|3671\|emb\|CAA47731.1\| p24b protein precursor [Saccharomyces cerevisiae] [NCBI NR] gi\|1322830\|emb\|CAA96912.1\| EMP24 [Saccharomyces cerevisiae] [NCBI NR] pir\|\|S25109 EMP24 protein precursor - yeast (Saccharomyces cerevisiae) [NCBI NR] Endosomal protein P24B OS=Saccharomyces cerevisiae GN=EMP24 PE=1 SV=1 [Swiss-Prot] (P32803) Endosomal P24B protein precursor (24 kDa endomembrane protein) (Basic 24 kDa late endocytic intermediate component) [Swiss-Prot] EMP24 Component of the COPII-coated vesicles, 24 kDa [mips] Integral membrane component of endoplasmic reticulum-derived COPII-coated vesicles, which function in ER to Golgi transport [SGD] EMP24, Chr VII from 122697-123308, reverse complement [yeast_orfs2] Chr VII from 122697-123308, reverse complement [yeastorf3.fasta] Chr VII from 123308-122697, reverse complement [yeastorf5.fasta] EMP24 SGDID:S0003168, Chr VII from 123308-122697, reverse complement, Verified ORF [yeastflysequest.fasta] EMP24 SGDID:S0003168, Chr VII from 123310-122699, reverse complement, Verified ORF [10112005cervSGD.fasta] Emp24p [Saccharomyces cerevisiae]gi\|1322830\|emb\|CAA96912.1\| EMP24 [Saccharomyces cerevisiae]gi\|3671\|emb\|CAA47731.1\| p24b protein precursor [Saccharomyces cerevisiae]gi\|417435\|sp\|P32803\|EM24_YEAST Endosomal P24B protein precursor (24 kDa endomembrane pr [nrdb_11152004_Chlamydomonas] EMP24 SGDID:S000003168, Chr VII from 123310-122699, reverse complement, Verified ORF, "Integral membrane component of endoplasmic reticulum-derived COPII-coated vesicles, which function in ER to Golgi transport" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] p24b protein precursor [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Endosomal protein P24B precursor (24 kDa endomembrane protein) (Basic 24 kDa late endocytic intermediate component) [nr-271106-contam.fasta] EMP24 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Integral membrane component of endoplasmic reticulum-derived COPII-coated vesicles, which function in ER to Golgi transport; Emp24p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] [chemostatdb.fasta] Endosomal protein P24B OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=EMP24 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] EMP24 SGDID:S000003168, Chr VII from 123305-122694, Genome Release 64-1-1, reverse complement, Verified ORF, "Component of the p24 complex; binds to GPI anchor proteins and mediates their efficient transport from the ER to the Golgi; integral membrane protein that associates with endoplasmic reticulum-derived COPII-coated vesicles" [yeast-030211-contam.fasta] EMP24 SGDID:S000003168, Chr VII from 123305-122694, Genome Release 64-2-1, reverse complement, Verified ORF, "Component of the p24 complex; role in misfolded protein quality control; binds to GPI anchor proteins and mediates their efficient transport from the ER to the Golgi; integral membrane protein that associates with endoplasmic reticulum-derived COPII-coated vesicles" [20141208_orf_trans.fasta] TR8144\|c0_g1_i1\|g.5642 ORF TR8144\|c0_g1_i1\|g.5642 TR8144\|c0_g1_i1\|m.5642 type:complete len:204 (+) TR8144\|c0_g1_i1:116-727(+) [Y12_S_cerevisiae-Sep2015-contam.fasta] TR6384\|c0_g1_i1\|g.4560 ORF TR6384\|c0_g1_i1\|g.4560 TR6384\|c0_g1_i1\|m.4560 type:complete len:204 (+) TR6384\|c0_g1_i1:122-733(+) [DBVPG6044_S_cerevisiae-Sep2015-contam.fasta] YGL200C SGDID:S000003168, Chr VII from 123305-122694, Genome Release 64-1-1, reverse complement, Verified ORF, "Component of the p24 complex; binds to GPI anchor proteins and mediates their efficient transport from the ER to the Golgi; integral membrane protein that associates with endoplasmic reticulum-derived COPII-coated vesicles" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta] asmbl_2034\|g.1542 ORF asmbl_2034\|g.1542 asmbl_2034\|m.1542 type:complete len:204 (+) asmbl_2034:122-733(+) [DBVPG6044_Trinity_v2_2_0_PASA_6May2016-contam.fasta] asmbl_2114\|g.961 ORF asmbl_2114\|g.961 asmbl_2114\|m.961 type:complete len:204 (+) asmbl_2114:116-727(+) [Y12_Trinity_v2_2_0_PASA_6May2016-contam.fasta] ID=asmbl_2037\|g.1281 ORF ID=asmbl_2037\|g.1281 ID=asmbl_2037\|m.1281 type:complete len:204 (+) ID=asmbl_2037:122-733(+) [DBVPG6044_quiverGenomes_Trinity_v2_2_0_PASA_2June2016-contam.fasta] ID=asmbl_2118\|g.1262 ORF ID=asmbl_2118\|g.1262 ID=asmbl_2118\|m.1262 type:complete len:204 (+) ID=asmbl_2118:116-727(+) [Y12_quiver_Trinity_v2_2_0_PASA_2June2016-contam.fasta]
Organism:	Saccharomyces cerevisiae
Length:	203 amino acids
Gene Ontology:
Cellular Component:	integral to membrane [ISS] endoplasmic reticulum [IDA] ER to Golgi transport vesicle [IDA]
Biological Process:	ER to Golgi vesicle-mediated transport [IMP][IPI] vesicle organization [IGI][IMP] protein retention in ER lumen [IMP]
Molecular Function:	molecular_function [ND]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MASFATKFVI ACFLFFSASA HNVLLPAYGR RCFFEDLSKG DELSISFQFG DRNPQSSSQL  60
   61 TGDFIIYGPE RHEVLKTVRD TSHGEITLSA PYKGHFQYCF LNENTGIETK DVTFNIHGVV 120
  121 YVDLDDPNTN TLDSAVRKLS KLTREVKDEQ SYIVIRERTH RNTAESTNDR VKWWSIFQLG 180
  181 VVIANSLFQI YYLRRFFEVT SLV

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		EMP24 ERP1 ERP2 ERV25
View Details	(MIPS) Mewes HW, et al. (2004)		EMP24 ERP1 ERP2 ERV25 ERV46 SAR1 SEC13 SEC16 SEC23 SEC24 SEC31
View Details	Krogan NJ, et al. (2006)		EMP24 ERP1 ERP2 KAR2 PHB2 SCJ1 SSS1 YEL1
View Details	Gavin AC, et al. (2006)		EMP24 ERP1 ERP2 ERV25
View Details	Gavin AC, et al. (2002)		ARO1 ARX1 EMP24 ERP1 ERP2 ERV25 FKS1 HAS1 HHF1, HHF2 KAP123 NIP7 PHB1 RRP1
View Details	Ho Y, et al. (2002)		EMP24 KGD1 KRE6 RPN1 RPN12 RPN5 RPN6 RPN8 RPN9 RPT1 RPT2
View Details	Qiu et al. (2008)		EMP24 ERP1 ERP2 ERV25 ERV41 ERV46 RER1 SAR1 SEC13 SEC16 SEC23 SEC24 SEC31 SFB3 SVP26 VRG4 YIP3 YOS1

Mass Spectrometry Data

No mass spectrometry results found for this protein.

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	RAD6	EMP24	2	Unpublished Fields Lab Data
View Screen	KSS1	EMP24	1	Unpublished Fields Lab Data
View Screen	APC5	EMP24	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	EMP24	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..203]	Pfam	81.387216	emp24/gp25L/p24 family/GOLD No confident structure predictions are available.

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
voltage-gated cation channel activity	2.66540214971227	bayes_pls_golite062009
substrate-specific channel activity	2.66283817406605	bayes_pls_golite062009
passive transmembrane transporter activity	2.65916906094646	bayes_pls_golite062009
channel activity	2.65916906094646	bayes_pls_golite062009
cation channel activity	2.62747969257897	bayes_pls_golite062009
potassium channel activity	2.61371256192732	bayes_pls_golite062009
voltage-gated ion channel activity	2.60719363833593	bayes_pls_golite062009
ion channel activity	2.60082350471123	bayes_pls_golite062009
voltage-gated channel activity	2.58448165457823	bayes_pls_golite062009
voltage-gated potassium channel activity	2.57450407680312	bayes_pls_golite062009
gated channel activity	2.51455369837435	bayes_pls_golite062009
metal ion transmembrane transporter activity	2.48447488950861	bayes_pls_golite062009
ligand-gated ion channel activity	2.48200622858578	bayes_pls_golite062009
ligand-gated channel activity	2.48200622858578	bayes_pls_golite062009
transporter activity	2.35657262831396	bayes_pls_golite062009
clathrin binding	2.30826135071113	bayes_pls_golite062009
transmembrane transporter activity	2.25147059981728	bayes_pls_golite062009
substrate-specific transporter activity	2.22751878261824	bayes_pls_golite062009
binding	2.16129867759095	bayes_pls_golite062009
substrate-specific transmembrane transporter activity	2.14804562881998	bayes_pls_golite062009
ion transmembrane transporter activity	2.08500207462933	bayes_pls_golite062009
cation transmembrane transporter activity	2.02275538762334	bayes_pls_golite062009
inward rectifier potassium channel activity	1.9397436784802	bayes_pls_golite062009
molecular transducer activity	1.58932339453245	bayes_pls_golite062009
signal transducer activity	1.58932339453245	bayes_pls_golite062009
protein binding	1.40069187357924	bayes_pls_golite062009
cytoskeletal protein binding	0.921436605338431	bayes_pls_golite062009
actin binding	0.852579554611056	bayes_pls_golite062009

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

[View Details]

Source: Reynolds et al. 2008. Manuscript submitted

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2