Protein Overview: CFT1

General Information:
Name(s) found:	CFT1 / YDR301W [SGD]
Description(s) found: Found 27 descriptions. SHOW ALL	RNA-binding subunit of the mRNA cleavage and polyadenylation factor; involved in poly(A) site recognition and required for both pre-mRNA cleavage and polyadenylation, 51% sequence similarity with mammalian AAUAA-binding subunit of CPSF [SGD]
Organism:	Saccharomyces cerevisiae
Length:	1357 amino acids
Gene Ontology:
Cellular Component:	mRNA cleavage and polyadenylation specificity factor complex [IPI ] mitochondrion [IDA ] mRNA cleavage factor complex [IPI]
Biological Process:	termination of RNA polymerase II transcription [IMP] mRNA polyadenylation [IDA][TAS] mRNA cleavage [IDA][TAS]
Molecular Function:	RNA binding [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MNVYDDVLDA TVVSHSLATH FTTSDYEELL VVRTNILSVY RPTRDGKLYL TDEFKFHGLI  60
   61 TDIGLIPQKD SPLSCLLLCT GVAKISILKF NTLTNSIDTL SLHYYEGKFK GKSLVELAKI 120
  121 STLRMDPGSS CALLFNNDII AFLPFHVNKN DDDEEEEDED ENIDDSELIH SMNQKSQGTN 180
  181 TFNKRKRTKL GDKFTAPSVV LVASELYEGA KNIIDIQFLK NFTKPTIALL YQPKLVWAGN 240
  241 TTISKLPTQY VILTLNIQPA ESATKIESTT IAFVKELPWD LHTIVPVSNG AIIVGTNELA 300
  301 FLDNTGVLQS TVLLNSFADK ELQKTKIINN SSLEIMFREK NTTSIWIPSS KSKNGGSNND 360
  361 ETLLLMDLKS NIYYIQMEAE GRLLIKFDIF KLPIVNDLLK ENSNPKCITR LNATNSNKNM 420
  421 DLFIGFGSGN ALVLRLNNLK STIETREAHN PSSGTNSLMD INDDDDEEMD DLYADEAPEN 480
  481 GLTTNDSKGT VETVQPFDIE LLSSLRNVGP ITSLTVGKVS SIDDVVKGLP NPNKNEYSLV 540
  541 ATSGNGSGSH LTVIQTSVQP EIELALKFIS ITQIWNLKIK GRDRYLITTD STKSRSDIYE 600
  601 SDNNFKLHKG GRLRRDATTV YISMFGEEKR IIQVTTNHLY LYDTHFRRLT TIKFDYEVIH 660
  661 VSVMDPYILV TVSRGDIKIF ELEEKNKRKL LKVDLPEILN EMVITSGLIL KSNMCNEFLI 720
  721 GLSKSQEEQL LFTFVTADNQ IIFFTKDHND RIFQLNGVDQ LNESLYISTY QLGDEIVPDP 780
  781 SIKQVMINKL GHDNKEEYLT ILTFGGEIYQ YRKLPQRRSR FYRNVTRNDL AITGAPDNAY 840
  841 AKGVSSIERI MHYFPDYNGY SVIFVTGSVP YILIKEDDST PKIFKFGNIP LVSVTPWSER 900
  901 SVMCVDDIKN ARVYTLTTDN MYYGNKLPLK QIKISNVLDD YKTLQKLVYH ERAQLFLVSY 960
  961 CKRVPYEALG EDGEKVIGYD ENVPHAEGFQ SGILLINPKS WKVIDKIDFP KNSVVNEMRS1020
 1021 SMIQINSKTK RKREYIIAGV ANATTEDTPP TGAFHIYDVI EVVPEPGKPD TNYKLKEIFQ1080
 1081 EEVSGTVSTV CEVSGRFMIS QSQKVLVRDI QEDNSVIPVA FLDIPVFVTD SKSFGNLLII1140
 1141 GDAMQGFQFI GFDAEPYRMI SLGRSMSKFQ TMSLEFLVNG GDMYFAATDA DRNVHVLKYA1200
 1201 PDEPNSLSGQ RLVHCSSFTL HSTNSCMMLL PRNEEFGSPQ VPSFQNVGGQ VDGSVFKIVP1260
 1261 LSEEKYRRLY VIQQQIIDRE LQLGGLNPRM ERLANDFYQM GHSMRPMLDF NVIRRFCGLA1320
 1321 IDRRKSIAQK AGRHAHFEAW RDIINIEFSM RSLCQGK

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		CFT1 CFT2 FIP1 GLC7 MPE1 PAP1 PFS2 PTA1 PTI1 REF2 RNA14 SSU72 SWD2 YSH1 YTH1
View Details	(MIPS) Mewes HW, et al. (2004)		CFT1 CFT2 PTA1 YSH1
View Details	Krogan NJ, et al. (2006)		AIM1 CFT1 CFT2 ECM21 FIP1 GIP4 MPE1 PAP1 PFS2 PTA1 PTI1 REF2 SSU72 SWD2 SYC1 YBL010C YLR358C YSH1 YTH1
View Details	Gavin AC, et al. (2006)		CFT1 CFT2 FIP1 MPE1 PAP1 PFS2 PTA1 YSH1 YTH1
View Details	Gavin AC, et al. (2002)		ACT1 AIM31 CFT1 CFT2 CKA1 CLP1 ENO2 FIP1 GLC7 HHF1, HHF2 HTA1 MPE1 PAP1 PCF11 PFK1 PFS2 PTA1 PTI1 REF2 RNA14 RNA15 RSA3 SEC13 SEC31 SSA3 SSU72 SYC1 TAF6 TIF4632 TYE7 VID24 VMR1 VPS53 YCL046W YSH1 YTH1
View Details	Ho Y, et al. (2002)		CCT5 CCT6 CFT1 CIC1 CLU1 ERB1 FIN1 FIP1 FPR3 FPR4 GLC7 GLC8 GPH1 HER1 HGH1 KEL1 MHP1 NSA1 PFS2 REG1 RRP17 SCD5 SDS22 SEN1 SPB1 SUI2 SUI3 TCP1 YTM1
View Details	Qiu et al. (2008)		CFT1 CFT2 CLP1 FIP1 HRP1 MPE1 PAP1 PFS2 PTA1 PTI1 REF2 RNA14 RNA15 SSU72 SWD2 SYC1 YSH1 YTH1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	RLF2	phosphorylation data - cascade search	Green EM, et al (2005)
View Run	BOI1	Sample boi 1 with ha tag from october 2005	McCusker D, et al (2007)
View Run	MLP2	#12 Mitotic Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#02 Alpha Factor Prep1-TiO2 enriched, new search criteria	Keck JM, et al. (2011)
View Run	MLP2	#30 Asynchronous Prep6-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	APC9	CFT1	2	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	CFT1	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..722]	MSA	2.02988	View MSA. No confident structure predictions are available.
2	View Details	[723..986]	deduced	N/A	No confident structure predictions are available.
3	View Details	[987..1357]	Pfam	126.568636	CPSF A subunit region No confident structure predictions are available.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.98

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2