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Protein Overview: PFK2

General Information:
Name(s) found: PFK2 / YMR205C [SGD]
Description(s) found:
Found 23 descriptions.
SHOW ALL
  • Beta subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes [SGD]
    • Organism: Saccharomyces cerevisiae
    Length: 959 amino acids

    Gene Ontology:
    Cellular Component: 6-phosphofructokinase complex [IMPLink to PubMed article]
    cytoplasm [IDALink to PubMed article]
    mitochondrion [IDALink to PubMed article]
    Biological Process: glycolysis [IDALink to PubMed article][IMPLink to PubMed article]
    Molecular Function: 6-phosphofructokinase activity [IMPLink to PubMed article]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MTVTTPFVNG TSYCTVTAYS VQSYKAAIDF YTKFLSLENR SSPDENSTLL SNDSISLKIL  60
   61 LRPDEKINKN VEAHLKELNS ITKTQDWRSH ATQSLVFNTS DILAVKDTLN AMNAPLQGYP 120
  121 TELFPMQLYT LDPLGNVVGV TSTKNAVSTK PTPPPAPEAS AESGLSSKVH SYTDLAYRMK 180
  181 TTDTYPSLPK PLNRPQKAIA VMTSGGDAPG MNSNVRAIVR SAIFKGCRAF VVMEGYEGLV 240
  241 RGGPEYIKEF HWEDVRGWSA EGGTNIGTAR CMEFKKREGR LLGAQHLIEA GVDALIVCGG 300
  301 DGSLTGADLF RSEWPSLIEE LLKTNRISNE QYERMKHLNI CGTVGSIDND MSTTDATIGA 360
  361 YSALDRICKA IDYVEATANS HSRAFVVEVM GRNCGWLALL AGIATSADYI FIPEKPATSS 420
  421 EWQDQMCDIV SKHRSRGKRT TIVVVAEGAI AADLTPISPS DVHKVLVDRL GLDTRITTLG 480
  481 HVQRGGTAVA YDRILATLQG LEAVNAVLES TPDTPSPLIA VNENKIVRKP LMESVKLTKA 540
  541 VAEAIQAKDF KRAMSLRDTE FIEHLNNFMA INSADHNEPK LPKDKRLKIA IVNVGAPAGG 600
  601 INSAVYSMAT YCMSQGHRPY AIYNGWSGLA RHESVRSLNW KDMLGWQSRG GSEIGTNRVT 660
  661 PEEADLGMIA YYFQKYEFDG LIIVGGFEAF ESLHQLERAR ESYPAFRIPM VLIPATLSNN 720
  721 VPGTEYSLGS DTALNALMEY CDVVKQSASS TRGRAFVVDC QGGNSGYLAT YASLAVGAQV 780
  781 SYVPEEGISL EQLSEDIEYL AQSFEKAEGR GRFGKLILKS TNASKALSAT KLAEVITAEA 840
  841 DGRFDAKPAY PGHVQQGGLP SPIDRTRATR MAIKAVGFIK DNQAAIAEAR AAEENFNADD 900
  901 KTISDTAAVV GVKGSHVVYN SIRQLYDYET EVSMRMPKVI HWQATRLIAD HLVGRKRVD

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details Riffle et al. (2010) (Unpublished Data) PFK1 PFK2
    View Details (MIPS) Mewes HW, et al. (2004) PFK1 PFK2
    View Details Krogan NJ, et al. (2006) PFK1 PFK2
    View Details Gavin AC, et al. (2006) PFK1 PFK2 RPS10A RPS22A
    View Details Gavin AC, et al. (2002) ADA2 ADH1 CLU1 FUN19 GCN1 GCN5 HEM13 ISW1 KAP114 KAP123 MED4 MES1 MNN4 MOT1 MYO1 NGG1 NOP1 PDC1 PFK1 PFK2 PSK1 PTC1 PUF6 RPB2 RPN9 RPO21 RPT5 RVB2 SGF29 SGF73 SIN3 SIN4 SPT15 SPT20 SPT7 SPT8 TAF1 TAF10 TAF11 TAF12 TAF13 TAF14 TAF2 TAF3 TAF4 TAF5 TAF6 TAF7 TAF8 TAF9 TBF1 TEF4 VAS1 VPS1 YEF3
    View Details Ho Y, et al. (2002) ACS2 ADE3 ADE6 ADO1 ALA1 APE2 ARA1 BAT1 CBF5 CDC10 CLU1 CYS3 DED81 DPS1 ERG10 ERG20 FPR1 GCY1 GPH1 GRS1 HOM2 HOM3 HYP2 ILS1 IMD3 KRS1 LSP1 MDH3 MLP2 OLA1 PAB1 PFK1 PFK2 PGM2 PMI40 PRP6 RNR2 SAC6 SAP155 SCP160 SHQ1 SIT4 SOD1 SRP1 SSZ1 TAP42 THR4 THS1 TIF1, TIF2 TRR1 UBA1 URA1 YDL124W YDR341C
    View Details Qiu et al. (2008) GLK1 HXK2 PFK1 PFK2

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run ALG13 No Comments Hazbun TR, et al. (2003)
    View Run UTP23 No Comments Hazbun TR, et al. (2003)
    View Run NSE3 No Comments Hazbun TR, et al. (2003)
    View Run PAN1 No Comments Toshima J, et al (2006)
    View Run RPA135 No Comments Schneider, DA, et al. (2006)
    View Run ZDS1 Sample 2- cpn2 from june 2005 McCusker D, et al (2007)
    View Run ZDS1 Sample 3 - cpn + atp, from june 2005 McCusker D, et al (2007)
    View Run BOI1 Sample boi 1 with ha tag from october 2005 McCusker D, et al (2007)
    View Run BOI1 Sample boi1 with gst from october 2005 McCusker D, et al (2007)
    View Run BOI1 Sample bob1 (2nd set) from october 2005 McCusker D, et al (2007)
    View Run MLP2 #19 Asynchronous Prep1-TiO2 Phosphopeptide enrichment Keck JM, et al. (2011)
    View Run MLP2 #25 Asynchronous Prep3-TiO2 Flowthrough Keck JM, et al. (2011)
    View Run MLP2 #24 Asynchronous Prep3-TiO2 Phosphopeptide enrichment Keck JM, et al. (2011)
    View Run MLP2 #26 Asynchronous Prep4-TiO2 Phosphopeptide enriched, Steps1-2 Keck JM, et al. (2011)
    View Run MLP2 #32 Asynchronous Prep-No Phosphopeptide enrichment Keck JM, et al. (2011)
    View Run MLP2 #35 Asynchronous SPB prep Keck JM, et al. (2011)
    View Run MLP2 #04 Alpha Factor Prep2 Keck JM, et al. (2011)
    View Run MLP2 #29 Asynchronous Prep5-TiO2 Flowthrough Keck JM, et al. (2011)
    View Run MLP2 #12 Mitotic Prep1-TiO2 Flowthrough Keck JM, et al. (2011)
    View Run MLP2 #10 Mitotic Prep1-TiO2 Phosphopeptide enrichment Keck JM, et al. (2011)
    View Run MLP2 #11 Mitotic Prep1-TiO2 enriched, new search criteria Keck JM, et al. (2011)
    View Run MLP2 #19b Asynchronous Prep1-new search criteria Keck JM, et al. (2011)
    View Run MLP2 #19a Asynchronous Prep1-new search criteria Keck JM, et al. (2011)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    NOT SHOWING SINGLE HITS. [ Show Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

    No yeast two-hybrid interactions found for this protein.

    Microscopy / Localization Data

    New Feature: Upload Your Own Microscopy Data

      PROTEIN(S) PUBLICATION
    View Data PFK2 Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..185] deduced N/A No confident structure predictions are available.
    2 View Details [186..363]
    [483..539]    		 PSI-BLAST 635.9897 ATP-dependent phosphofructokinase
    3 View Details [364..482]
    [540..548]    		 PSI-BLAST 635.9897 ATP-dependent phosphofructokinase
    4 View Details [549..827] PSI-BLAST 112.08661 Pyrophosphate-dependent phosphofructokinase
    5 View Details [828..959] ORFEUS 12.5 ATP-dependent phosphofructokinase

    Functions predicted (by domain):

    # Gene Ontology predictions
    1 No functions predicted.
    2
    Term Confidence Notes
  • 6-phosphofructokinase activity
    		•  12.7403693686468 bayes_pls_golite062009
  • phosphofructokinase activity
    		•  11.7059647240147 bayes_pls_golite062009
  • fructose-6-phosphate binding
    		•  9.55959144901554 bayes_pls_golite062009
  • fructose binding
    		•  9.55841643584799 bayes_pls_golite062009
  • carbohydrate kinase activity
    		•  9.54757838113048 bayes_pls_golite062009
  • phosphotransferase activity, alcohol group as acceptor
    		•  3.19280659830412 bayes_pls_golite062009
  • kinase activity
    		•  2.85624705588769 bayes_pls_golite062009
  • fructose-2,6-bisphosphate 2-phosphatase activity
    		•  2.7198911999025 bayes_pls_golite062009
  • transferase activity, transferring phosphorus-containing groups
    		•  2.67472251964197 bayes_pls_golite062009
  • sugar binding
    		•  2.21212411210899 bayes_pls_golite062009
  • monosaccharide binding
    		•  2.05358532613083 bayes_pls_golite062009
  • transferase activity
    		•  1.97789134877808 bayes_pls_golite062009
  • catalytic activity
    		•  1.89212073381079 bayes_pls_golite062009
  • protein binding
    		•  1.76136621968843 bayes_pls_golite062009
  • binding
    		•  1.57437723650762 bayes_pls_golite062009
  • carbohydrate binding
    		•  0.97067862054315 bayes_pls_golite062009
  • hydrolase activity
    		•  0.276140444059183 bayes_pls_golite062009
    3
    Term Confidence Notes
  • 6-phosphofructokinase activity
    		•  12.7403693686468 bayes_pls_golite062009
  • phosphofructokinase activity
    		•  11.7059647240147 bayes_pls_golite062009
  • fructose-6-phosphate binding
    		•  9.55959144901554 bayes_pls_golite062009
  • fructose binding
    		•  9.55841643584799 bayes_pls_golite062009
  • carbohydrate kinase activity
    		•  9.54757838113048 bayes_pls_golite062009
  • phosphotransferase activity, alcohol group as acceptor
    		•  3.19280659830412 bayes_pls_golite062009
  • kinase activity
    		•  2.85624705588769 bayes_pls_golite062009
  • fructose-2,6-bisphosphate 2-phosphatase activity
    		•  2.7198911999025 bayes_pls_golite062009
  • transferase activity, transferring phosphorus-containing groups
    		•  2.67472251964197 bayes_pls_golite062009
  • sugar binding
    		•  2.21212411210899 bayes_pls_golite062009
  • monosaccharide binding
    		•  2.05358532613083 bayes_pls_golite062009
  • transferase activity
    		•  1.97789134877808 bayes_pls_golite062009
  • catalytic activity
    		•  1.89212073381079 bayes_pls_golite062009
  • protein binding
    		•  1.76136621968843 bayes_pls_golite062009
  • binding
    		•  1.57437723650762 bayes_pls_golite062009
  • carbohydrate binding
    		•  0.97067862054315 bayes_pls_golite062009
  • hydrolase activity
    		•  0.276140444059183 bayes_pls_golite062009
    4 No functions predicted.
    5 No functions predicted.


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.91

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle