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Bait Protein: MLP2
Organism: Saccharomyces cerevisiae
Publication: Keck JM, Jones MH, Wong CC, Binkley J, Chen D, Jaspersen SL, Holinger EP, Xu T, Niepel M, Rout MP, Vogel J, Sidow A, Yates JR, Winey M., A Cell Cycle Phosphoproteome of the Yeast Centrosome, Science 24 June 2011: 1557-1561.
Comments: #35 Asynchronous SPB prep

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UNFILTERED Run Results: [filter]

[View our MS interpretation guidelines.]
  Hit Protein Protein Desc Sequence Count Spectrum Count Sequence Coverage NSAF Mol. Wt.
[View Peptides]   YIL002W-A Putative protein of unknown function; identified by expression profiling and mass spectrometry 8 8 79.7% 0.264 7729
[View Peptides]   CMD1 Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, act... 3 4 55.1% 0.0619 16135
[View Peptides]   SPC42 Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate att... 14 15 46.0% 0.094 42271
[View Peptides]   SPA1_STAAU (P02976) Immunoglobulin G binding protein A precursor (IgG binding protein A) (Staphylococcal protei... 18 25 31.1% 0.108 57320
[View Peptides]   CNM67 Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclea... 9 10 23.4% 0.0391 67400
[View Peptides]   CDC31 Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication i... 3 3 16.8% 0.0424 18751
[View Peptides]   MLP2 Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the... 26 36 16.4% 0.0488 195140
[View Peptides]   SPC29 Inner plaque spindle pole body (SPB) component, links the central plaque component Spc42p to the inn... 4 8 16.2% 0.0719 29280
[View Peptides]   SPC110 Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nu... 14 17 15.4% 0.041 111782
[View Peptides]   NUD1 Component of the spindle pole body outer plaque, required for exit from mitosis 13 15 14.6% 0.0401 94104
[View Peptides]   BBP1 Protein required for the spindle pole body (SPB) duplication, localized at the central plaque periph... 4 4 14.0% 0.0236 45384
[View Peptides]   BFA1 Component of the GTPase-activating Bfa1p-Bub2p complex involved in multiple cell cycle checkpoint pa... 3 3 10.6% 0.0119 66087
[View Peptides]   CDC14 Protein phosphatase required for mitotic exit; located in the nucleolus until liberated by the FEAR ... 3 3 9.8% 0.0124 61907
[View Peptides]   RPL5 Protein component of the large (60S) ribosomal subunit with similarity to E. coli L18 and rat L5 rib... 1 1 9.4% 0.00766 33715
[View Peptides]   MET28 Basic leucine zipper (bZIP) transcriptional activator in the Cbf1p-Met4p-Met28p complex, participate... 1 1 9.1% 0.0122 21590
[View Peptides]   gi|2119236, gi|4... sp|P35527|K1CI_HUMAN Keratin, type I cytoskeletal 9 (Cytokeratin 9) (K9) (CK 9), gi|545257|gb|AAC60... 2 2 8.7% 0.00731 61987
[View Peptides]   RPS3 Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease ... 1 1 7.1% 0.00947 26503
[View Peptides]   SSB1 Cytoplasmic ATPase that is a ribosome-associated molecular chaperone, functions with J-protein partn... 2 2 6.9% 0.00742 66602
[View Peptides]   SSB2 Cytoplasmic ATPase that is a ribosome-associated molecular chaperone, functions with J-protein partn... 2 2 6.9% 0.00742 66595
[View Peptides]   YCR015C Putative protein of unknown function; YCR015C is not an essential gene 1 1 6.9% 0.00717 36278
[View Peptides]   SSA2 ATP binding protein involved in protein folding and vacuolar import of proteins; member of heat shoc... 2 2 6.6% 0.00712 69470
[View Peptides]   SSA1 ATPase involved in protein folding and nuclear localization signal (NLS)-directed nuclear transport;... 2 2 6.5% 0.00708 69768
[View Peptides]   RRB1 Essential nuclear protein involved in early steps of ribosome biogenesis; physically interacts with ... 1 1 6.3% 0.00445 57261
[View Peptides]   MRPL3 Mitochondrial ribosomal protein of the large subunit 1 1 6.2% 0.00583 44000
[View Peptides]   HSP42 Small heat shock protein (sHSP) with chaperone activity; forms barrel-shaped oligomers that suppress... 1 1 6.1% 0.00606 42817
[View Peptides]   NPL3 RNA-binding protein that promotes elongation, regulates termination, and carries poly(A) mRNA from n... 1 1 5.3% 0.00549 45407
[View Peptides]   TEF4 Translation elongation factor EF-1 gamma 1 1 5.1% 0.00552 46520
[View Peptides]   DEF1 RNAPII degradation factor, forms a complex with Rad26p in chromatin, enables ubiquitination and prot... 2 2 4.2% 0.00616 83973
[View Peptides]   NOP12 Nucleolar protein involved in pre-25S rRNA processing and biogenesis of large 60S ribosomal subunit;... 1 1 3.1% 0.00495 51942
[View Peptides]   YBR235W Putative ion transporter, similar to mammalian electroneutral Na(+)-(K+)-C1- cotransporter family; Y... 1 1 2.9% 0.00203 123998
[View Peptides]   Reverse_YPL256C CLN2 SGDID:S000006177, Chr XVI from 66614-64977, reverse complement, Verified ORF, "G1 cyclin involv... 1 1 2.9% 0.00416 61697
[View Peptides]   MPS2 Essential membrane protein localized at the nuclear envelope and spindle pole body (SPB), required f... 1 1 2.8% 0.00588 44585
[View Peptides]   Reverse_YDR484W VPS52 SGDID:S000002892, Chr IV from 1422753-1424678, Verified ORF, "Component of the GARP (Golgi-ass... 1 1 2.3% 0.00354 74333
[View Peptides]   PAM1 Essential protein of unknown function; exhibits variable expression during colony morphogenesis; ove... 1 2 2.2% 0.00548 92872
[View Peptides]   Reverse_YKR031C SPO14 SGDID:S000001739, Chr XI from 506037-500986, reverse complement, Verified ORF, "Phospholipase ... 1 1 1.9% 0.00135 195203
[View Peptides]   PFK2 Beta subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaero... 1 1 1.0% 0.00237 104618
[View Peptides]   Reverse_YNL139C RLR1 SGDID:S000005083, Chr XIV from 365719-360926, reverse complement, Verified ORF, "Subunit of the... 1 1 0.9% 0.00142 183930
[View Peptides]   FMP27 Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purifi... 1 1 0.6% 8.65E-4 303478
[View Peptides]   Reverse_YDL140C RPO21 SGDID:S000002299, Chr IV from 210562-205361, reverse complement, Verified ORF, "RNA polymerase... 1 1 0.6% 0.00131 191610
[View Peptides]   UBR2 Cytoplasmic ubiquitin-protein ligase (E3); required for ubiquitylation of Rpn4p; mediates formation ... 1 1 0.4% 0.00121 216777
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