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Bait Protein: PAN1
Organism: Saccharomyces cerevisiae
Publication: Toshima J, Toshima JY, Duncan MC, Cope MJ, Sun Y, Martin AC, Anderson S, Yates JR 3rd, Mizuno K, Drubin DG. Negative regulation of yeast Eps15-like Arp2/3 complex activator, Pan1p, by the Hip1R-related protein, Sla2p, during endocytosis. Mol Biol Cell. 2007 Feb;18(2):658-68. Epub 2006 Dec 6.
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  Hit Protein Protein Desc Sequence Count Spectrum Count Sequence Coverage NSAF Mol. Wt.
[View Peptides]   PAN1 Part of actin cytoskeleton-regulatory complex Pan1p-Sla1p-End3p, associates with actin patches on th... 605 2059 86.3% 0.467 160266
[View Peptides]   END3 EH domain-containing protein involved in endocytosis, actin cytoskeletal organization and cell wall ... 108 239 81.4% 0.23 40297
[View Peptides]   TOM22 Component of the TOM (translocase of outer mitochondrial membrane) complex responsible for initial i... 2 2 27.0% 0.00442 16790
[View Peptides]   PEX21 Peroxin required for targeting of peroxisomal matrix proteins containing PTS2; interacts with Pex7p;... 2 2 14.2% 0.00233 33050
[View Peptides]   FMP16 Putative protein of unknown function; proposed to be involved in responding to conditions of stress;... 2 2 12.9% 0.00722 10845
[View Peptides]   STP4 Protein containing a Kruppel-type zinc-finger domain; has similarity to Stp1p, Stp2p, and Stp3p 2 2 11.6% 0.00137 54367
[View Peptides]   NOP13 Nucleolar protein found in preribosomal complexes; contains an RNA recognition motif (RRM) 2 2 10.7% 0.00167 45667
[View Peptides]   GID8 Protein of unknown function, involved in proteasome-dependent catabolite inactivation of fructose-1,... 2 2 10.3% 0.00148 51729
[View Peptides]   SPT14 UDP-GlcNAc-binding and catalytic subunit of the enzyme that mediates the first step in glycosylphosp... 2 2 9.1% 0.00149 51242
[View Peptides]   GYP1 Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, ... 2 3 7.8% 0.00158 73289
[View Peptides]   HOF1 Bud neck-localized, SH3 domain-containing protein required for cytokinesis; regulates actomyosin rin... 2 2 7.5% 0.001 76207
[View Peptides]   YPR196W Putative maltose activator 2 2 6.8% 0.00143 55077
[View Peptides]   BOR1 Boron efflux transporter of the plasma membrane; binds HCO3-, I-, Br-, NO3- and Cl-; has similarity ... 2 2 6.4% 0.00117 65028
[View Peptides]   TRM44 tRNA(Ser) Um(44) 2'-O-methyltransferase; involved in maintaining levels of the tRNA-Ser species tS(C... 2 2 6.2% 0.00118 64855
[View Peptides]   CDC13 Single stranded DNA-binding protein found at TG1-3 telomere G-tails; regulates telomere replication ... 2 2 5.7% 7.26E-4 104904
[View Peptides]   YJR015W Putative protein of unknown function; localizes to the endoplasmic reticulum and cytoplasm; predicte... 2 3 5.5% 0.00197 58117
[View Peptides]   SLX5 Subunit of the Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex, stimulated by SUMO-modified... 2 2 5.3% 0.00108 71071
[View Peptides]   TMN2 Protein with a role in cellular adhesion and filamentous growth; similar to Emp70p and Tmn3p; member... 2 3 5.2% 0.0015 76347
[View Peptides]   TDA7 Cell cycle-regulated gene of unknown function, promoter bound by Fkh2p 2 2 5.0% 0.00106 67357
[View Peptides]   NOP12 Nucleolar protein involved in pre-25S rRNA processing and biogenesis of large 60S ribosomal subunit;... 2 2 5.0% 0.00146 51942
[View Peptides]   ZIP2 Meiosis-specific protein involved in normal synaptonemal complex formation and pairing between homol... 2 2 4.8% 9.54E-4 82816
[View Peptides]   YTA12 Component, with Afg3p, of the mitochondrial inner membrane m-AAA protease that mediates degradation ... 2 2 4.6% 8.14E-4 93276
[View Peptides]   STO1 Large subunit of the nuclear mRNA cap-binding protein complex, interacts with Npl3p to carry nuclear... 2 2 4.5% 7.8E-4 100017
[View Peptides]   VPS1 Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization,... 2 2 4.5% 9.54E-4 78736
[View Peptides]   YDR333C Putative protein of unknown function 2 2 4.4% 9.28E-4 83426
[View Peptides]   ACE2 Transcription factor that activates expression of early G1-specific genes, localizes to daughter cel... 2 2 4.3% 8.72E-4 86634
[View Peptides]   PHO81 Cyclin-dependent kinase (CDK) inhibitor, regulates Pho80p-Pho85p and Pcl7p-Pho85p cyclin-CDK complex... 2 28 4.3% 0.00798 134029
[View Peptides]   ZRG8 Protein of unknown function; authentic, non-tagged protein is detected in highly purified mitochondr... 3 3 4.2% 9.36E-4 119350
[View Peptides]   CYM1 Lysine-specific metalloprotease of the mitochondrial intermembrane space, member of the pitrilysin f... 2 2 4.2% 6.79E-4 112180
[View Peptides]   TCB2 Bud-specific protein with a potential role in membrane trafficking; GFP-fusion protein migrates from... 2 2 4.2% 5.7E-4 132509
[View Peptides]   GPI13 ER membrane localized phosphoryltransferase that adds phosphoethanolamine onto the third mannose res... 2 2 4.0% 6.6E-4 115634
[View Peptides]   TAF1 TFIID subunit (145 kDa), involved in RNA polymerase II transcription initiation; possesses in vitro ... 2 2 3.9% 6.3E-4 120696
[View Peptides]   CHD1 Nucleosome remodeling factor that functions in regulation of transcription elongation; contains a ch... 2 2 3.7% 4.57E-4 168240
[View Peptides]   UTP5 Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA a... 2 2 3.4% 0.00104 72002
[View Peptides]   SLK19 Kinetochore-associated protein required for normal segregation of chromosomes in meiosis and mitosis... 2 2 3.2% 8.18E-4 95380
[View Peptides]   DPB11 Replication initiation protein that loads DNA pol epsilon onto pre-replication complexes at origins;... 2 2 3.1% 8.79E-4 87241
[View Peptides]   IKI3 Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; main... 2 2 3.0% 4.98E-4 152990
[View Peptides]   GDE1 Glycerophosphocholine (GroPCho) phosphodiesterase; hydrolyzes GroPCho to choline and glycerolphospha... 3 3 2.9% 8.23E-4 138014
[View Peptides]   PSK2 One of two (see also PSK1) PAS domain containing S/T protein kinases; regulates sugar flux and trans... 2 2 2.9% 6.1E-4 124372
[View Peptides]   HCS1 Hexameric DNA polymerase alpha-associated DNA helicase A involved in lagging strand DNA synthesis; c... 2 2 2.9% 9.83E-4 78273
[View Peptides]   SPO14 Phospholipase D, catalyzes the hydrolysis of phosphatidylcholine, producing choline and phosphatidic... 2 2 2.7% 3.99E-4 195203
[View Peptides]   BUD2 GTPase activating factor for Rsr1p/Bud1p required for both axial and bipolar budding patterns; mutan... 2 2 2.7% 6.08E-4 126663
[View Peptides]   SPR3 Sporulation-specific homolog of the yeast CDC3/10/11/12 family of bud neck microfilament genes; sept... 2 2 2.7% 0.00131 59845
[View Peptides]   NOT3 Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcrip... 2 2 2.5% 8.03E-4 94403
[View Peptides]   ERD1 Predicted membrane protein required for the retention of lumenal endoplasmic reticulum proteins; mut... 2 2 2.5% 0.00185 43216
[View Peptides]   CIN1 Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased... 2 2 2.4% 6.62E-4 116648
[View Peptides]   ACC1 Acetyl-CoA carboxylase, biotin containing enzyme that catalyzes the carboxylation of acetyl-CoA to f... 3 4 2.3% 6.01E-4 250351
[View Peptides]   OSW2 Protein of unknown function proposed to be involved in the assembly of the spore wall 2 3 2.3% 0.00197 58995
[View Peptides]   HPF1 Haze-protective mannoprotein that reduces the particle size of aggregated proteins in white wines 2 2 2.2% 6.94E-4 94705
[View Peptides]   RIC1 Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that... 2 2 1.9% 6.36E-4 121649
[View Peptides]   LAA1 AP-1 accessory protein; colocalizes with clathrin to the late-Golgi apparatus; involved in TGN-endos... 2 2 1.8% 3.33E-4 229898
[View Peptides]   DOP1 Golgi-localized, leucine-zipper domain containing protein; involved in endosome to Golgi transport, ... 2 2 1.7% 3.95E-4 194686
[View Peptides]   IRA1 GTPase-activating protein that negatively regulates RAS by converting it from the GTP- to the GDP-bo... 2 2 1.7% 2.17E-4 350980
[View Peptides]   MYO1 Type II myosin heavy chain, required for wild-type cytokinesis and cell separation; localizes to the... 2 2 1.6% 3.48E-4 223634
[View Peptides]   RSE1 Protein involved in pre-mRNA splicing; component of the pre-spliceosome; associates with U2 snRNA; i... 2 2 1.4% 4.93E-4 153784
[View Peptides]   URB1 Nucleolar protein required for the normal accumulation of 25S and 5.8S rRNAs, associated with the 27... 2 2 1.2% 3.81E-4 203286
[View Peptides]   CSF1 Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected ... 2 2 1.0% 2.27E-4 338258
[View Peptides]   VPS13 Protein of unknown function; heterooligomeric or homooligomeric complex; peripherally associated wit... 2 2 0.7% 2.14E-4 357850
[View Peptides]   MDN1 Huge dynein-related AAA-type ATPase (midasin), forms extended pre-60S particle with the Rix1 complex... 2 3 0.4% 2.05E-4 559316

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