Protein Overview: CTF4

General Information:
Name(s) found:	CTF4 / YPR135W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6325393\|ref\|NP_015461.1\| Chromatin-associated protein, required for sister chromatid cohesion; interacts with DNA polymerase alpha (Pol1p) and may link DNA synthesis to sister chromatid cohesion; Ctf4p [Saccharomyces cerevisiae] [NCBI NR] gi\|400807\|sp\|Q01454.2\|CTF4_YEAST RecName: Full=DNA polymerase alpha-binding protein; AltName: Full=Chromosome transmission fidelity protein 4; AltName: Full=Protein POB1; AltName: Full=Chromosome replication protein CHL15 [NCBI NR] pir\|\|A45039 CTF4 protein - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|172207\|gb\|AAA34887.1\| initiating methionine is not found in the mature protein;the N-terminal sequence of the protein is VSVID [Saccharomyces cerevisiae] [NCBI NR] gi\|1066483\|gb\|AAB68276.1\| Ctf4p: DNA Polymerase alpha-binding protein (Swiss Prot. accession number Q01454) [Saccharomyces cerevisiae] [NCBI NR] DNA polymerase alpha-binding protein OS=Saccharomyces cerevisiae GN=CTF4 PE=1 SV=2 [Swiss-Prot] CTF4 DNA-directed DNA polymerase alpha-binding protein [mips] Chromatin-associated protein, required for sister chromatid cohesion; interacts with DNA polymerase alpha (Pol1p) and may link DNA synthesis to sister chromatid cohesion [SGD] Chr XVI from 799228-802011 [hmr_yeast5_DM3-1_pombe.fasta] Chr XVI from 799229-802012 [yeastorf3.fasta] CTF4, Chr XVI from 799229-802012 [yeast_orfs2] CTF4 SGDID:S0006339, Chr XVI from 799228-802011, Verified ORF [yeastflysequest.fasta] CTF4 SGDID:S0006339, Chr XVI from 799230-802013, Verified ORF [10112005cervSGD.fasta] Chromatin-associated protein, required for sister chromatid cohesion; interacts with DNA polymerase alpha (Pol1p) and may link DNA synthesis to sister chromatid cohesion [Saccharomyces cerevisiae]gi\|1066483\|gb\|AAB68276.1\| Ctf4p: DNA Polymerase alpha-bind [nrdb_11152004_Chlamydomonas] CTF4 SGDID:S000006339, Chr XVI from 799230-802013, Verified ORF, "Chromatin-associated protein, required for sister chromatid cohesion; interacts with DNA polymerase alpha (Pol1p) and may link DNA synthesis to sister chromatid cohesion" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] Chromatin-associated protein, required for sister chromatid cohesion; interacts with DNA polymerase alpha (Pol1p) and may link DNA synthesis to sister chromatid cohesion; Ctf4p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Ctf4p: DNA Polymerase alpha-binding protein (Swiss Prot. accession number Q01454) [Saccharomyces cerevisiae] [nr-271106-contam.fasta] initiating methionine is not found in the mature protein;the N-terminal sequence of the protein is VSVID [nr-271106-contam.fasta] DNA polymerase alpha-binding protein (Protein CTF4) (Protein POB1) (Chromosome replication protein CHL15) [nr-271106-contam.fasta] [chemostatdb.fasta] DNA polymerase alpha-binding protein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=CTF4 PE=1 SV=2 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] CTF4 SGDID:S000006339, Chr XVI from 799234-802017, Genome Release 64-1-1, Verified ORF, "Chromatin-associated protein, required for sister chromatid cohesion; interacts with DNA polymerase alpha (Pol1p) and may link DNA synthesis to sister chromatid cohesion" [yeast-030211-contam.fasta] CTF4 SGDID:S000006339, Chr XVI from 799234-802017, Genome Release 64-2-1, Verified ORF, "Chromatin-associated protein; required for sister chromatid cohesion; interacts with DNA polymerase alpha (Pol1p) and may link DNA synthesis to sister chromatid cohesion" [20141208_orf_trans.fasta] YPR135W SGDID:S000006339, Chr XVI from 799234-802017, Genome Release 64-1-1, Verified ORF, "Chromatin-associated protein, required for sister chromatid cohesion; interacts with DNA polymerase alpha (Pol1p) and may link DNA synthesis to sister chromatid cohesion" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	927 amino acids
Gene Ontology:
Cellular Component:	nucleus [IPI] nuclear chromosome [IDA]
Biological Process:	DNA repair [IGI ][IMP ] DNA-dependent DNA replication [IMP][IPI] mitotic sister chromatid cohesion [IGI][IMP] replicative cell aging [IMP]
Molecular Function:	DNA binding [ISS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MVSVIDKLVF DFGGKTLVSL APDNNTLCVA NKNGLTKILK TNNPEEEPET LDSSKLVSSI  60
   61 KCYSNSHFLM TTMQGDALRY NIDSSQEELL ARFALPLRDC CVIHSGKMAV FGGDDLELIL 120
  121 LELDDETHKK HAIKIDEQVS QISYNSQMNI LAVSMINGKV QIFSLTSTIP NKVHELNDYI 180
  181 VANSYDDTHR DKILSNMMDD IDKDNDNDLS ETADPDENNV ADPEFCAANR ICTRVAWHPK 240
  241 GLHFALPCAD DTVKIFSIKG YSLQKTLSTN LSSTKAHFID LQFDPLRGTY IAAVDLNNKL 300
  301 TVWNWETSEI HYTREFKRKI TNIAWKIQAD SKTLDLVLGT WSGSIAIVQN LAESVVSNIP 360
  361 DQSVAESSTK HGLFVDSESD LENLEGNDDI NKSDKLFSDI TQEANAEDVF TQTHDGPSGL 420
  421 SEKRKYNFED EEDFIDDDDG AGYISGKKPH NEHSYSRVHK THSFPISLAN TGKFRYMPFS 480
  481 PAGTPFGFTD RRYLTMNEVG YVSTVKNSEQ YSITVSFFDV GRFREYHFED LFGYDLCFLN 540
  541 EKGTLFGQSK TGQIQYRPHD SIHSNWTKII PLQAGERITS VAATPVRVIV GTSLGYFRSF 600
  601 NQFGVPFAVE KTSPIVALTA QNYRVFSVHY SQFHGLSYSL SELGTSSKRY YKRECPLPMS 660
  661 LPNINSDMKK DANLDYYNFN PMGIKSLFFS SYGDPCIFGS DNTLLLLSKW RSPEESKWLP 720
  721 ILDSNMEIWK MSGGKETTDI HVWPLALAYD TLNCILVKGK HIWPEFPLPL PSEMEIRMPV 780
  781 FVKSKLLEEN KAILNKKNEI GADTEAEEGE EDKEIQIPVS MAAEEEYLRS KVLSELLTDT 840
  841 LENDGEMYGN ENEVLAALNG AYDKALLRLF ASACSDQNVE KALSLAHELK QDRALTAAVK 900
  901 ISERAELPSL VKKINNIREA RYEQQLK

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Krogan NJ, et al. (2006)		CTF4 PSF1 PSF2 PSF3 SLD5
View Details	Ho Y, et al. (2002)		BMS1 CCE1 CDC53 CIC1 COF1 CTF4 DBP10 DED81 DIA2 ENP1 FPR3 ILS1 KRE33 KRI1 LST4 MCM2 MCM3 MCM5 NIP7 NMD3 NOP12 RAD1 RAD14 RAD16 RAD4 RRP12 SEH1 SKP1 SLT2 SPB1 SSF1 SSF2 SSZ1 TIF6 URB1 UTP10 YAK1 YBL104C

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	MLP2	#26 Asynchronous Prep4-TiO2 Phosphopeptide enriched, Steps1-2	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	PSF1	CTF4	2	Hazbun TR, et al. (2003)
View Screen	PSF1	CTF4	2	Hazbun TR, et al. (2003)
View Screen	PSF2	CTF4	2	Hazbun TR, et al. (2003)

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	CTF4	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..348]	PSI-BLAST	60.522879	Tup1, C-terminal domain
2	View Details	[349..468]	PSI-BLAST	7.69897	Surface layer protein
3	View Details	[469..538]	deduced	N/A	Confident ab initio structure predictions are available.
4	View Details	[539..794]	MSA	9.318997	View MSA. No confident structure predictions are available.
5	View Details	[795..927]	deduced	N/A	Confident ab initio structure predictions are available.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2