Protein Overview: HFI1

General Information:
Name(s) found:	HFI1 / YPL254W [SGD]
Description(s) found: Found 26 descriptions. SHOW ALL	Adaptor protein required for structural integrity of the SAGA complex, a histone acetyltransferase-coactivator complex that is involved in global regulation of gene expression through acetylation and transcription functions [SGD]
Organism:	Saccharomyces cerevisiae
Length:	488 amino acids
Gene Ontology:
Cellular Component:	SLIK (SAGA-like) complex [IPI] Ada2/Gcn5/Ada3 transcription activator complex [IDA][IPI] SAGA complex [IDA]
Biological Process:	chromatin modification [TAS] telomere maintenance [IMP] transcription from RNA polymerase II promoter [IMP] histone acetylation [TAS]
Molecular Function:	transcription cofactor activity [IMP][IPI] transcription coactivator activity [IMP]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSAIQSPAPK PLQPTYPAAS PASTNAYMKP GLIGSPAVSN HTEPNNGNNE TAEPQGPNQR  60
   61 IDLGAMIEEL TSLLGKESWT KYAQIISLFI LGKLSRKELS NELELVFSPS AASLEKSNTN 120
  121 HHHSLVRLHN QLLLGIFANS LRENPLGRNG NESSWGFGNG SNNPNNKLKR INKHNSQIEV 180
  181 YKKIVMSLPL NDRNRLKMIT KEAGKRGFIF CSVFQARLNN IPKIPIVTNP ESLKRVKSNN 240
  241 LKTPLEWSQD IMNGFNVPLA SESHSLPDTD SFYLRMVGIA REHGLVGTVD ARCVELISLA 300
  301 LDQYLKNIIE FTIDTVRYRR KKYSDYYDLN ESGLYKSVSE MAADKRDAKI KQLDDDKNED 360
  361 ECADEAKSIN NGNNSSKDDI GDISMSSITK AGEAVNEELH ENRTISLTNE DIYDSLSIFP 420
  421 NLVEPSGSYY ALTNLGLVND DELVDMKSNI DDLPDFLNEK PTFTPLDERN VGTRHELNWL 480
  481 IKGILTED

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		ADA2 GCN5 HFI1 NGG1 SGF11 SGF29 SGF73 SPT20 SPT3 SPT7 SPT8 TAF1 TAF10 TAF11 TAF12 TAF3 TAF4 TAF5 TAF6 TAF7 TAF9 TRA1 UBP8
View Details	(MIPS) Mewes HW, et al. (2004)		ADA2 GCN5 HFI1 NGG1 SGF29 SGF73 SPT20 SPT3 SPT7 SPT8 TAF10 TAF12 TAF5 TAF6 TAF9 TRA1
View Details	Krogan NJ, et al. (2006)		ADA2 AHC1 AHC2 FMP40 GCN5 HFI1 MSS4 NGG1 PDR15 RAD59 REV3 SGF11 SGF29 SGF73 SNL1 SPT20 SPT3 SPT7 SPT8 TAF1 TAF10 TAF11 TAF12 TAF13 TAF3 TAF4 TAF5 TAF6 TAF7 TAF9 UBP8 YMR007W
View Details	Gavin AC, et al. (2006)		ADA2 HFI1 NGG1 SGF29 SGF73 SPT20 SPT3 SPT7 SPT8
View Details	Gavin AC, et al. (2002)		ADA2 GCN5 HEM13 HFI1 MES1 MYO4 NGG1 PSK1 PTC1 RPB2 RPO21 SGF29 SGF73 SPT20 SPT7 SPT8 TAF1 TAF10 TAF11 TAF12 TAF3 TAF5 TAF6 TAF7 TAF9 UBP8 VAS1
View Details	Ho Y, et al. (2002)		ADA2 ARP4 FAA2 FET4 GCN5 HFI1 RPO31 SGF29 SPT7 STB4 SWC4 SWI5 TAF6 TRA1 UBP8
View Details	Qiu et al. (2008)		ADA2 AHC1 ARP4 CHD1 GCN5 HFI1 MED1 MED2 NGG1 PGD1 SGF11 SGF29 SGF73 SIN4 SPT20 SPT3 SPT7 SPT8 SRB5 SRB6 SRB7 SUS1 TAF1 TAF10 TAF11 TAF12 TAF13 TAF14 TAF3 TAF5 TAF6 TAF8 TAF9 TFB5 TRA1 UBP8

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	CEP3	sample cbf3 from feb 2005	Sandall S, et all (2006)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	SWI5	HFI1	1	Unpublished Fields Lab Data
View Screen	PCF11	HFI1	1	Unpublished Fields Lab Data
View Screen	YNL260C	HFI1	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	HFI1	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..322]	MSA	5.011972	View MSA. No confident structure predictions are available.
2	View Details	[323..488]	deduced	N/A	No confident structure predictions are available.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.97

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2