Protein Overview: TUF1

General Information:
Name(s) found:	TUF1 / YOR187W [SGD]
Description(s) found: Found 27 descriptions. SHOW ALL	Mitochondrial translation elongation factor Tu; comprises both GTPase and guanine nucleotide exchange factor activities, while these activities are found in separate proteins in S. pombe and humans [SGD]
Organism:	Saccharomyces cerevisiae
Length:	437 amino acids
Gene Ontology:
Cellular Component:	mitochondrial matrix [ISS] mitochondrion [IDA]
Biological Process:	translational elongation [IGI ][ISS]
Molecular Function:	translation elongation factor activity [IGI][ISS ] GTPase activity [IGI][ISS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSALLPRLLT RTAFKASGKL LRLSSVISRT FSQTTTSYAA AFDRSKPHVN IGTIGHVDHG  60
   61 KTTLTAAITK TLAAKGGANF LDYAAIDKAP EERARGITIS TAHVEYETAK RHYSHVDCPG 120
  121 HADYIKNMIT GAAQMDGAII VVAATDGQMP QTREHLLLAR QVGVQHIVVF VNKVDTIDDP 180
  181 EMLELVEMEM RELLNEYGFD GDNAPIIMGS ALCALEGRQP EIGEQAIMKL LDAVDEYIPT 240
  241 PERDLNKPFL MPVEDIFSIS GRGTVVTGRV ERGNLKKGEE LEIVGHNSTP LKTTVTGIEM 300
  301 FRKELDSAMA GDNAGVLLRG IRRDQLKRGM VLAKPGTVKA HTKILASLYI LSKEEGGRHS 360
  361 GFGENYRPQM FIRTADVTVV MRFPKEVEDH SMQVMPGDNV EMECDLIHPT PLEVGQRFNI 420
  421 REGGRTVGTG LITRIIE

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Krogan NJ, et al. (2006)		ADE12 TUF1
View Details	Gavin AC, et al. (2006)		CCT5 SEN54 TUF1
View Details	Ho Y, et al. (2002)		APL2 APL4 APM1 ARP4 ATE1 BRX1 CDC33 CIC1 CKA2 EAF1 EPL1 ESA1 HRR25 KRI1 KRR1 NMA1 NOG1 NOP4 PAB1 POR1 PWP1 RCL1 SAP185 SIT4 TAF14 TAF4 TIF6 TIP41 TRA1 TUF1 YRA1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	VAM3	Sample: HX12 - trans-SNARE complex formation and fusion are inhibited (control).	Hao Xu, et al. (2010)
View Run	MLP2	#31 Asynchronous Prep (Protease cleavage)	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	CHL4	TUF1	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	TUF1	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..44] [243..437]	PSI-BLAST	1652.0	Elongation factor Tu (EF-Tu), domain 2; Elongation factor Tu (EF-Tu); Elongation factor Tu (EF-Tu), N-terminal (G) domain
2	View Details	[45..242]	PSI-BLAST	1652.0	Elongation factor Tu (EF-Tu), domain 2; Elongation factor Tu (EF-Tu); Elongation factor Tu (EF-Tu), N-terminal (G) domain

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
translation elongation factor activity	8.90683166637832	bayes_pls_golite062009
structural constituent of ribosome	7.90308112669861	bayes_pls_golite062009
structural molecule activity	7.31087460425911	bayes_pls_golite062009
translation factor activity, nucleic acid binding	6.75036567581468	bayes_pls_golite062009
translation regulator activity	6.7106575654195	bayes_pls_golite062009
transporter activity	3.10463051760206	bayes_pls_golite062009
transmembrane transporter activity	2.95886887664984	bayes_pls_golite062009
nucleoside-triphosphatase activity	2.95079405147356	bayes_pls_golite062009
pyrophosphatase activity	2.87427509025394	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides	2.85499564954033	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	2.85178667290149	bayes_pls_golite062009
translation initiation factor activity	2.68619850021832	bayes_pls_golite062009
active transmembrane transporter activity	2.50494515048649	bayes_pls_golite062009
primary active transmembrane transporter activity	2.39629638016861	bayes_pls_golite062009
P-P-bond-hydrolysis-driven transmembrane transporter activity	2.39600931083427	bayes_pls_golite062009
ATPase activity	2.39535714892224	bayes_pls_golite062009
nucleic acid binding	2.39500449599674	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances	2.36051733655256	bayes_pls_golite062009
ATPase activity, coupled to movement of substances	2.35906629925754	bayes_pls_golite062009
ATPase activity, coupled to transmembrane movement of substances	2.35679406876731	bayes_pls_golite062009
ATPase activity, coupled	2.24901251684901	bayes_pls_golite062009
binding	2.01866595777668	bayes_pls_golite062009
substrate-specific transporter activity	1.65609907941094	bayes_pls_golite062009
hydrolase activity	1.57549510726226	bayes_pls_golite062009
motor activity	1.34881124154297	bayes_pls_golite062009
substrate-specific transmembrane transporter activity	1.3182108176243	bayes_pls_golite062009
translation release factor activity	1.27090696996316	bayes_pls_golite062009
translation termination factor activity	1.2623252798648	bayes_pls_golite062009
DNA binding	0.967303417236485	bayes_pls_golite062009
RNA binding	0.798696391797967	bayes_pls_golite062009
transcription regulator activity	0.748437190903548	bayes_pls_golite062009
peptidyltransferase activity	0.705667095586631	bayes_pls_golite062009
ion transmembrane transporter activity	0.704934361725919	bayes_pls_golite062009
microtubule motor activity	0.60036517876321	bayes_pls_golite062009
cytoskeletal protein binding	0.500462538667228	bayes_pls_golite062009
GTPase activity	0.437665353870877	bayes_pls_golite062009
ATP binding	0.41092997612902	bayes_pls_golite062009
adenyl ribonucleotide binding	0.385813421701492	bayes_pls_golite062009
adenyl nucleotide binding	0.377870145186619	bayes_pls_golite062009
anion transmembrane-transporting ATPase activity	0.353150780751661	bayes_pls_golite062009
cation transmembrane transporter activity	0.282397764628576	bayes_pls_golite062009
protein binding	0.242047208982959	bayes_pls_golite062009
RNA helicase activity	0.2302541318658	bayes_pls_golite062009
inorganic anion transmembrane transporter activity	0.12168417344278	bayes_pls_golite062009
microfilament motor activity	0.10236059675512	bayes_pls_golite062009
protein transporter activity	0.0397092337273124	bayes_pls_golite062009
protein transmembrane transporter activity	0.0378638259240902	bayes_pls_golite062009
peptide transporter activity	0.0135927378126404	bayes_pls_golite062009
actin binding	0.00482938540552702	bayes_pls_golite062009
signal sequence binding	6.53725193420218E-4	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

[View Details]

Source: Reynolds et al. 2008. Manuscript submitted

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2