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Protein Overview: PEP5

General Information:
Name(s) found: PEP5 / YMR231W [SGD]
Description(s) found:
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  • gi|887612|emb|CAA90202.1| End1p [Saccharomyces cerevisiae] [NCBI NR]
  • gi|666102|emb|CAA38348.1| vacuolar biogenesis protein END1 (PEP5) protein [Saccharomyces cerevisiae] [NCBI NR]
  • gi|6323887|ref|NP_013958.1| Component of CORVET tethering complex; peripheral vacuolar membrane protein required for protein trafficking and vacuole biogenesis; interacts with Pep7p; Pep5p [Saccharomyces cerevisiae] [NCBI NR]
  • pir||S57598 PEP5 protein - yeast (Saccharomyces cerevisiae) [NCBI NR]
  • gi|129788|sp|P12868.2|PEP5_YEAST RecName: Full=Vacuolar membrane protein PEP5; AltName: Full=Vacuolar biogenesis protein END1; AltName: Full=Vacuolar protein sorting-associated protein 11; AltName: Full=Vacuolar morphogenesis protein 1; AltName: Full=Vacu [NCBI NR]
  • Vacuolar membrane protein PEP5 OS=Saccharomyces cerevisiae GN=PEP5 PE=1 SV=2 [Swiss-Prot]
  • (P12868) Vacuolar biogenesis protein END1 (PEP5 protein) (Vacuolar protein sorting 11) [Swiss-Prot]
  • PEP5 Vacuolar biogenesis protein [mips]
  • Component of CORVET tethering complex; peripheral vacuolar membrane protein required for protein trafficking and vacuole biogenesis; interacts with Pep7p [SGD]
  • PEP5, Chr XIII from 733544-736633 [yeast_orfs2]
  • Chr XIII from 733544-736633 [yeastorf3.fasta]
  • PEP5 SGDID:S0004844, Chr XIII from 733544-736633, Verified ORF [yeastflysequest.fasta]
  • Pep5p [Saccharomyces cerevisiae]gi|887612|emb|CAA90202.1| End1p [Saccharomyces cerevisiae]gi|666102|emb|CAA38348.1| vacuolar biogenesis protein END1 (PEP5) protein [Saccharomyces cerevisiae]gi|129788|sp|P12868|END1_YEAST Vacuolar biogenesis protein END [nrdb_11152004_Chlamydomonas]
  • PEP5 SGDID:S000004844, Chr XIII from 733544-736633, Verified ORF, "Peripheral vacuolar membrane protein required for protein trafficking and vacuole biogenesis; forms complex with Pep3p that promotes vesicular docking/fusion reactions in conjunction with [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta]
  • PEP5 SGDID:S000004844, Chr XIII from 733544-736633, Verified ORF, "Component of CORVET tethering complex; peripheral vacuolar membrane protein required for protein trafficking and vacuole biogenesis; interacts with Pep7p" [SGD_S-cerevisiae_na_08-19-2008_con_reversed.fasta]
  • vacuolar biogenesis protein END1 (PEP5) protein [Saccharomyces cerevisiae] [nr-271106-contam.fasta]
  • End1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta]
  • Vacuolar membrane protein PEP5 (Vacuolar biogenesis protein END1) (Vacuolar protein sorting-associated protein 11) (Vacuolar morphogenesis protein 1) (Vacuolar protein-targeting protein 11) (Carboxypeptidase Y-deficient protein 5) [nr-271106-contam.fasta]
  • Peripheral vacuolar membrane protein required for protein trafficking and vacuole biogenesis; forms complex with Pep3p that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, also interacts with Pep7p; Pep5p [Saccharomyces cer [nr-271106-contam.fasta]
  • PEP5 SGDID:S000004844, Chr XIII from 733544-736633, Verified ORF, "Peripheral vacuolar membrane protein required for protein trafficking and vacuole biogenesis; forms complex with Pep3p that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, also interacts with Pep7p" [yeast-contam_custom.fasta]
  • [chemostatdb.fasta]
  • Vacuolar membrane protein PEP5 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PEP5 PE=1 SV=2 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta]
  • PEP5 SGDID:S000004844, Chr XIII from 733545-736634, Genome Release 64-1-1, Verified ORF, "Component of CORVET tethering complex; peripheral vacuolar membrane protein required for protein trafficking and vacuole biogenesis; interacts with Pep7p" [yeast-030211-contam.fasta]
  • PEP5 SGDID:S000004844, Chr XIII from 733545-736634, Genome Release 64-2-1, Verified ORF, "Histone E3 ligase, component of CORVET membrane tethering complex; peripheral vacuolar membrane protein required for protein trafficking and vacuole biogenesis; interacts with Pep7p; involved in ubiquitylation and degradation of excess histones" [20141208_orf_trans.fasta]
  • YMR231W SGDID:S000004844, Chr XIII from 733545-736634, Genome Release 64-1-1, Verified ORF, "Component of CORVET tethering complex; peripheral vacuolar membrane protein required for protein trafficking and vacuole biogenesis; interacts with Pep7p" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
  • E3 ubiquitin-protein ligase PEP5 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PEP5 PE=1 SV=2 [uniprot_sprot.fasta.20160308]
    • Organism: Saccharomyces cerevisiae
    Length: 1029 amino acids

    Gene Ontology:
    Cellular Component: fungal-type vacuole membrane [IDALink to PubMed article]
    HOPS complex [IPILink to PubMed article]
    Biological Process: vesicle docking during exocytosis [IMPLink to PubMed article]
    late endosome to vacuole transport [IGILink to PubMed article]
    vacuole fusion, non-autophagic [IDALink to PubMed article]
    Golgi to endosome transport [IGILink to PubMed article]
    Molecular Function: molecular_function [ND]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSLSSWRQFQ LFENIPIRDP NFGGDSLLYS DPTLCAATIV DPQTLIIAVN SNIIKVVKLN  60
   61 QSQVIHEFQS FPHDFQITFL KVINGEFLVA LAESIGKPSL IRVYKLEKLP NREQLYHSQV 120
  121 ELKNGNNTYP ISVVSISNDL SCIVVGFING KIILIRGDIS RDRGSQQRII YEDPSKEPIT 180
  181 ALFLNNDATA CFAATTSRIL LFNTTGRNRG RPSLVLNSKN GLDLNCGSFN PATNEFICCL 240
  241 SNFIEFFSSS GKKHQFAFDL SLRKRIFCVD KDHILIVTEE TGVPTTSISV NELSPTIINR 300
  301 IFIIDAKNKI ISLNFVVSSA IIDIFSTSQS GKNITYLLTS EGVMHRITPK SLENQINIII 360
  361 QKELYPFALQ LAKQHSLSPL DVQEIHKKYG DYLFKKGLRK EATDQYIQCL DVVETSEIIS 420
  421 KFGVKEVPDP ESMRNLADYL WSLIKNSISQ RDHVTLLLIV LIKLKDVEGI DTFIQHFDRK 480
  481 GIWNEGVVMD DMDDVTFFYS DNDFFDLDLI LELMKESDFK RLSYRLAKKY SKDSLIIVDI 540
  541 LLNLLHNPVK AIKYIKSLPI DETLRCLVTY SKKLLEESPN ETNALLIEVF TGKFKPSTFE 600
  601 VDLDRRDTTG DFSENIRTVF YSYKTFFNYM NSNGTSDAMS ESSEASHEHE EPTYHPPKPS 660
  661 IVFSSFVTKP FEFVVFLEAC LACYQQYEGF DEDRQVILTT LYDLYLNLAQ NDVPERIDDW 720
  721 RSRATGVLRE SNKLVYSAAS NNTSKRVDNS IMLLISHMDQ SSASAKDKTK IDIASFANDN 780
  781 PEMDLLSTFR AMTLNEEPST CLKFLEKYGT EEPKLLQVAL SYFVSNKLIF KEMGGNEVLK 840
  841 EKVLRPIIEG ERMPLLDIIK ALSRTNVAHF GLIQDIIIDH VKTEDTEIKR NEKLIESYDK 900
  901 ELKEKNKKLK NTINSDQPLH VPLKNQTCFM CRLTLDIPVV FFKCGHIYHQ HCLNEEEDTL 960
  961 ESERKLFKCP KCLVDLETSN KLFEAQHEVV EKNDLLNFAL NSEEGSRDRF KVITEFLGRG1020
 1021 AISYSDITI

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details Riffle et al. (2010) (Unpublished Data) PEP3 PEP5 VAM6 VPS16 VPS33 VPS41 VPS8
    View Details (MIPS) Mewes HW, et al. (2004) PEP3 PEP5 VPS16 VPS33
    View Details Gavin AC, et al. (2006) PEP3 PEP5 VAM6 VPS16 VPS33 VPS41 VPS8
    View Details Gavin AC, et al. (2002) ATP8 LUC7 PEP3 PEP5 SNU71 VAM6 VPS16 VPS33 VPS41 VPS8
    View Details Qiu et al. (2008) PEP3 PEP5 VPS16

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run CEP3 sample cbf3 from feb 2005 Sandall S, et all (2006)
    View Run ZDS1 Sample 2- cpn2 from june 2005 McCusker D, et al (2007)
    View Run ZDS1 Sample 3 - cpn + atp, from june 2005 McCusker D, et al (2007)
    View Run VAM3 sample: hx3 Hao Xu, et al. (2010)
    View Run VAM3 Sample: HX11 - both fusion and trans-SNARE complex formation take place. Hao Xu, et al. (2010)
    View Run VAM3 Sample: HX12 - trans-SNARE complex formation and fusion are inhibited (control). Hao Xu, et al. (2010)
    View Run VAM3 Sample: HX13 - trans-SNARE complex forms but fusion is blocked. Hao Xu, et al. (2010)
    View Run VAM3 Sample: HX14 - mixture in detergent (control). Hao Xu, et al. (2010)
    View Run MLP2 #29 Asynchronous Prep5-TiO2 Flowthrough Keck JM, et al. (2011)
    View Run None Entered #17 Mitotic Prep3-TiO2 Phosphopeptide enrichment Keck JM, et al. (2011)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    NOT SHOWING SINGLE HITS. [ Show Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

    No yeast two-hybrid interactions found for this protein.

    Microscopy / Localization Data

    New Feature: Upload Your Own Microscopy Data

      PROTEIN(S) PUBLICATION
    View Data PEP5 Huh WK, et al. (2003)
    View Data PEP5 and SNF7 Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..377] deduced N/A No confident structure predictions are available.
    2 View Details [378..635] Pfam 21.136677 Region in Clathrin and VPS No confident structure predictions are available.
    3 View Details [636..865] deduced N/A No confident structure predictions are available.
    4 View Details [866..1029] PSI-BLAST 2.69897 bard1 RING domain

    Functions predicted (by domain):

    # Gene Ontology predictions
    1
    Term Confidence Notes
  • ubiquitin-protein ligase activity
    		•  3.27972671455235 bayes_pls_golite062009
  • small conjugating protein ligase activity
    		•  3.00024678477589 bayes_pls_golite062009
  • acid-amino acid ligase activity
    		•  2.09205299847945 bayes_pls_golite062009
  • binding
    		•  1.75605065075676 bayes_pls_golite062009
  • transporter activity
    		•  1.23232041140488 bayes_pls_golite062009
  • protein binding
    		•  1.04520986427713 bayes_pls_golite062009
  • substrate-specific transporter activity
    		•  0.750051725890322 bayes_pls_golite062009
  • phosphotransferase activity, alcohol group as acceptor
    		•  0.450377149626952 bayes_pls_golite062009
  • kinase activity
    		•  0.41337453952205 bayes_pls_golite062009
  • hydrolase activity
    		•  0.346903609858078 bayes_pls_golite062009
  • cytoskeletal protein binding
    		•  0.271959658140432 bayes_pls_golite062009
  • transferase activity, transferring phosphorus-containing groups
    		•  0.260136536525518 bayes_pls_golite062009
  • ligase activity, forming carbon-nitrogen bonds
    		•  0.190416660410399 bayes_pls_golite062009
  • nucleic acid binding
    		•  0.103339839445939 bayes_pls_golite062009
  • nucleoside-triphosphatase activity
    		•  0.0245411309982146 bayes_pls_golite062009
    2
    Term Confidence Notes
  • protein transporter activity
    		•  2.99345877012947 bayes_pls_golite062009
  • transporter activity
    		•  2.3766833486706 bayes_pls_golite062009
  • binding
    		•  2.33303322187387 bayes_pls_golite062009
  • substrate-specific transporter activity
    		•  2.12926593993985 bayes_pls_golite062009
  • protein binding
    		•  1.5114863464349 bayes_pls_golite062009
  • transmembrane transporter activity
    		•  1.2724388835449 bayes_pls_golite062009
  • protein transmembrane transporter activity
    		•  1.12112006227201 bayes_pls_golite062009
  • substrate-specific transmembrane transporter activity
    		•  1.04982314606251 bayes_pls_golite062009
  • hydrolase activity
    		•  0.566325388018401 bayes_pls_golite062009
  • phosphotransferase activity, alcohol group as acceptor
    		•  0.535131417738445 bayes_pls_golite062009
  • kinase activity
    		•  0.507502362811171 bayes_pls_golite062009
  • nucleic acid binding
    		•  0.497783403092344 bayes_pls_golite062009
  • transferase activity, transferring phosphorus-containing groups
    		•  0.416234300339629 bayes_pls_golite062009
  • signal sequence binding
    		•  0.279693431859311 bayes_pls_golite062009
  • active transmembrane transporter activity
    		•  0.260663498540354 bayes_pls_golite062009
  • ion transmembrane transporter activity
    		•  0.256036243669218 bayes_pls_golite062009
  • transferase activity
    		•  0.249515205994519 bayes_pls_golite062009
  • primary active transmembrane transporter activity
    		•  0.234271600707329 bayes_pls_golite062009
  • P-P-bond-hydrolysis-driven transmembrane transporter activity
    		•  0.225395946682209 bayes_pls_golite062009
  • hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances
    		•  0.186601655036333 bayes_pls_golite062009
  • ATPase activity, coupled to movement of substances
    		•  0.183072614802483 bayes_pls_golite062009
  • ATPase activity, coupled to transmembrane movement of substances
    		•  0.182751938544977 bayes_pls_golite062009
  • nucleoside-triphosphatase activity
    		•  0.109435859778099 bayes_pls_golite062009
  • clathrin binding
    		•  0.0923079381755934 bayes_pls_golite062009
  • pyrophosphatase activity
    		•  0.0728493557966827 bayes_pls_golite062009
  • hydrolase activity, acting on acid anhydrides
    		•  0.0641324729536009 bayes_pls_golite062009
  • hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides
    		•  0.0615190129942244 bayes_pls_golite062009
  • cation transmembrane transporter activity
    		•  0.0475280280823402 bayes_pls_golite062009
  • protein kinase activity
    		•  0.0308998889737299 bayes_pls_golite062009
    3
    Term Confidence Notes
  • ubiquitin-protein ligase activity
    		•  5.97121728620188 bayes_pls_golite062009
  • small conjugating protein ligase activity
    		•  5.78935175140238 bayes_pls_golite062009
  • acid-amino acid ligase activity
    		•  4.75377804653841 bayes_pls_golite062009
  • ligase activity, forming carbon-nitrogen bonds
    		•  2.57382484274962 bayes_pls_golite062009
  • binding
    		•  2.15929427993458 bayes_pls_golite062009
  • protein binding
    		•  1.49775478023418 bayes_pls_golite062009
  • SUMO polymer binding
    		•  0.805553743681771 bayes_pls_golite062009
  • small conjugating protein-specific protease activity
    		•  0.786612602432575 bayes_pls_golite062009
  • ligase activity
    		•  0.63517530468004 bayes_pls_golite062009
  • hydrolase activity
    		•  0.626901453756812 bayes_pls_golite062009
  • ubiquitin-specific protease activity
    		•  0.549461161056205 bayes_pls_golite062009
  • cysteine-type peptidase activity
    		•  0.159023519781458 bayes_pls_golite062009
  • catalytic activity
    		•  0.14112008370434 bayes_pls_golite062009
  • nucleic acid binding
    		•  0.125491277540945 bayes_pls_golite062009
  • transcription regulator activity
    		•  0.0833655277418337 bayes_pls_golite062009
    4 No functions predicted.


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.96

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle