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Protein Overview: MNN1

General Information:
Name(s) found: MNN1 / YER001W [SGD]
Description(s) found:
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  • gi|730039|sp|P39106.1|MNN1_YEAST RecName: Full=Alpha-1,3-mannosyltransferase MNN1 [NCBI NR]
  • gi|6320837|ref|NP_010916.1| Alpha-1,3-mannosyltransferase, integral membrane glycoprotein of the Golgi complex, required for addition of alpha1,3-mannose linkages to N-linked and O-linked oligosaccharides, one of five S. cerevisiae proteins of the MNN1 fa [NCBI NR]
  • gi|603593|gb|AAB64534.1| Mnn1p: alpha-1,3-mannosyltransferase [Saccharomyces cerevisiae] [NCBI NR]
  • pir||S50459 MNN1 protein - yeast (Saccharomyces cerevisiae) [NCBI NR]
  • Alpha-1,3-mannosyltransferase MNN1 OS=Saccharomyces cerevisiae GN=MNN1 PE=1 SV=1 [Swiss-Prot]
  • MNN1 Alpha-1,3-mannosyltransferase [mips]
  • Alpha-1,3-mannosyltransferase, integral membrane glycoprotein of the Golgi complex, required for addition of alpha1,3-mannose linkages to N-linked and O-linked oligosaccharides, one of five S. cerevisiae proteins of the MNN1 family [SGD]
  • MNN1, Chr V from 153519-155807 [yeast_orfs2]
  • Chr V from 153519-155807 [yeastorf3.fasta]
  • (P39106) Alpha-1,3-mannosyltransferase MNN1 (EC 2.4.1.-) [4932.FASTACCERV.txt]
  • MNN1 SGDID:S0000803, Chr V from 153519-155807, Verified ORF [yeastflysequest.fasta]
  • Alpha-1,3-mannosyltransferase, integral membrane glycoprotein of the Golgi complex, required for addition of alpha1,3-mannose linkages to N-linked and O-linked oligosaccharides, one of five S. cerevisiae proteins of the MNN1 family [Saccharomyces cerevisi [nrdb_11152004_Chlamydomonas]
  • MNN1 SGDID:S000000803, Chr V from 153519-155807, Verified ORF, "Alpha-1,3-mannosyltransferase, integral membrane glycoprotein of the Golgi complex, required for addition of alpha1,3-mannose linkages to N-linked and O-linked oligosaccharides, one of five S [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta]
  • Alpha-1,3-mannosyltransferase, integral membrane glycoprotein of the Golgi complex, required for addition of alpha1,3-mannose linkages to N-linked and O-linked oligosaccharides, one of five S. cerevisiae proteins of the MNN1 family; Mnn1p [Saccharomyces c [nr-271106-contam.fasta]
  • Mnn1p: alpha-1,3-mannosyltransferase [Saccharomyces cerevisiae] [nr-271106-contam.fasta]
  • Alpha-1,3-mannosyltransferase MNN1 [nr-271106-contam.fasta]
  • MNN1 SGDID:S000000803, Chr V from 153519-155807, Verified ORF, "Alpha-1,3-mannosyltransferase, integral membrane glycoprotein of the Golgi complex, required for addition of alpha1,3-mannose linkages to N-linked and O-linked oligosaccharides, one of five S. cerevisiae proteins of the MNN1 family" [yeast-bovrabprots.fasta]
  • [chemostatdb.fasta]
  • Alpha-1,3-mannosyltransferase MNN1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=MNN1 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta]
  • MNN1 SGDID:S000000803, Chr V from 153520-155808, Genome Release 64-1-1, Verified ORF, "Alpha-1,3-mannosyltransferase, integral membrane glycoprotein of the Golgi complex, required for addition of alpha1,3-mannose linkages to N-linked and O-linked oligosaccharides, one of five S. cerevisiae proteins of the MNN1 family" [yeast-030211-contam.fasta]
  • MNN1 SGDID:S000000803, Chr V from 153520-155808, Genome Release 64-2-1, Verified ORF, "Alpha-1,3-mannosyltransferase; integral membrane glycoprotein of the Golgi complex, required for addition of alpha1,3-mannose linkages to N-linked and O-linked oligosaccharides, one of five S. cerevisiae proteins of the MNN1 family" [20141208_orf_trans.fasta]
  • YER001W SGDID:S000000803, Chr V from 153520-155808, Genome Release 64-1-1, Verified ORF, "Alpha-1,3-mannosyltransferase, integral membrane glycoprotein of the Golgi complex, required for addition of alpha1,3-mannose linkages to N-linked and O-linked oligosaccharides, one of five S. cerevisiae proteins of the MNN1 family" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
    • Organism: Saccharomyces cerevisiae
    Length: 762 amino acids

    Gene Ontology:
    Cellular Component: Golgi apparatus [IDALink to PubMed article]
    Biological Process: protein amino acid O-linked glycosylation [IDALink to PubMed article]
    N-glycan processing [IMPLink to PubMed article]
    Molecular Function: alpha-1,3-mannosyltransferase activity [IMPLink to PubMed article]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MLALRRFILN QRSLRSCTIP ILVGALIIIL VLFQLVTHRN DALIRSSNVN STNKKTLKDA  60
   61 DPKVLIEAFG SPEVDPVDTI PVSPLELVPF YDQSIDTKRS SSWLINKKGY YKHFNELSLT 120
  121 DRCKFYFRTL YTLDDEWTNS VKKLEYSIND NEGVDEGKDA NGNPMDEKSE RLYRRKYDMF 180
  181 QAFERIRAYD RCFMQANPVN IQEIFPKSDK MSKERVQSKL IKTLNATFPN YDPDNFKKYD 240
  241 QFEFEHKMFP FINNFTTETF HEMVPKITSP FGKVLEQGFL PKFDHKTGKV QEYFKYEYDP 300
  301 SKTFWANWRD MSAKVAGRGI VLSLGSNQFP LAVKFIASLR FEGNTLPIQV VYRGDELSQE 360
  361 LVDKLIYAAR SPDFKPVENN YDNSTNVPQE IWFLDVSNTI HPKWRGDFGS YKSKWLVVLL 420
  421 NLLQEFVFLD IDAISYEKID NYFKTTEYQK TGTVFYRERA LRENVNERCI ARYETLLPRN 480
  481 LESKNFQNSL LIDPDHALNE CDNTLTTEEY IFKAFFHHRR QHQLEAGLFA VDKSKHTIPL 540
  541 VLAAMIHLAK NTAHCTHGDK ENFWLGFLAA GHTYALQGVY SGAIGDYVKK TDLNGKRQEA 600
  601 AVEICSGQIA HMSTDKKTLL WVNGGGTFCK HDNAAKDDWK KDGDFKKFKD QFKTFEEMEK 660
  661 YYYITPISSK YVILPDPKSD DWHRASAGAC GGYIWCATHK TLLKPYSYNH RTTHGELITL 720
  721 DEEQRLHIDA VNTVWSHANK DNTRSFTEEE IKELENSRHE QS

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details Riffle et al. (2010) (Unpublished Data) KTI11 MNN1
    View Details Krogan NJ, et al. (2006) AIM22 DPH1 DPH2 EFT2, EFT1 HGH1 MNN1 PGI1 RRG10 TRX1 YMR315W
    View Details Qiu et al. (2008) ANP1 GNT1 KRE2 KTR1 KTR4 MNN1 MNN11

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run TOR2 No Comments Reinke A, et al. (2004)
    View Run RPA135 his-HA tag on RPA135 Schneider, DA, et al. (2006)
    View Run CEP3 sample cbf3 from feb 2005 Sandall S, et all (2006)
    View Run ASF1 3829 - low filter Green EM, et al (2005)
    View Run RPA135 No Comments Schneider, DA, et al. (2006)
    View Run VAM3 sample: hx3 Hao Xu, et al. (2010)
    View Run VAM3 Sample: HX11 - both fusion and trans-SNARE complex formation take place. Hao Xu, et al. (2010)
    View Run VAM3 Sample: HX12 - trans-SNARE complex formation and fusion are inhibited (control). Hao Xu, et al. (2010)
    View Run VAM3 Sample: HX14 - mixture in detergent (control). Hao Xu, et al. (2010)
    View Run VAM3 hx23: mixture in detergent (control) Investigating Vam3p, Nyv1p and their partners in trans-SNARE complex Hao Xu, et al. (2010)
    View Run MLP2 #20 Asynchronous Prep1-TiO2 Phosphopeptide enrichment, PartB Keck JM, et al. (2011)
    View Run MLP2 #25 Asynchronous Prep3-TiO2 Flowthrough Keck JM, et al. (2011)
    View Run MLP2 #29 Asynchronous Prep5-TiO2 Flowthrough Keck JM, et al. (2011)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    SHOWING SINGLE HITS. [ Hide Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

      BAIT PREY HITS PUBLICATION
    View Screen CIT2 MNN1 1 Unpublished Fields Lab Data
    View Screen SAM35 MNN1 1 Unpublished Fields Lab Data
    View Screen UBC7 MNN1 1 Unpublished Fields Lab Data

    Microscopy / Localization Data

    New Feature: Upload Your Own Microscopy Data

      PROTEIN(S) PUBLICATION
    View Data MNN1 Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..233] deduced N/A No confident structure predictions are available.
    2 View Details [234..762] MSA 2.008843 View MSA. No confident structure predictions are available.
    3 View Details [302..762] FFAS03 1.51 apo form of the 162S mutant of glycogenin

    Functions predicted (by domain):

    # Gene Ontology predictions
    1 No functions predicted.
    2 No functions predicted.
    3
    Term Confidence Notes
  • mannosyltransferase activity
    		•  5.1732667004955 bayes_pls_golite062009
  • transferase activity, transferring hexosyl groups
    		•  4.02812104984198 bayes_pls_golite062009
  • transferase activity, transferring glycosyl groups
    		•  4.02134669395634 bayes_pls_golite062009
  • UDP-glycosyltransferase activity
    		•  3.59185576348855 bayes_pls_golite062009
  • alpha-1,2-mannosyltransferase activity
    		•  3.3757785630345 bayes_pls_golite062009
  • acetylgalactosaminyltransferase activity
    		•  2.9474291454591 bayes_pls_golite062009
  • polypeptide N-acetylgalactosaminyltransferase activity
    		•  2.69652752452892 bayes_pls_golite062009
  • transferase activity
    		•  2.3313878748424 bayes_pls_golite062009
  • transferase activity, transferring phosphorus-containing groups
    		•  1.79751747857925 bayes_pls_golite062009
  • catalytic activity
    		•  1.73979371332125 bayes_pls_golite062009
  • binding
    		•  1.28147782130114 bayes_pls_golite062009
  • nucleotidyltransferase activity
    		•  0.778320684712752 bayes_pls_golite062009


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    [View Details]
    Source: Reynolds et al. 2008. Manuscript submitted Philius confidence legend
    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle