Protein Overview: GCD6

General Information:
Name(s) found:	GCD6 / YDR211W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6320417\|ref\|NP_010497.1\| Catalytic epsilon subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 exp [NCBI NR] pir\|\|A48156 translation regulator GCD6 - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|417035\|sp\|P32501.1\|EI2BE_YEAST RecName: Full=Translation initiation factor eIF-2B subunit epsilon; AltName: Full=eIF-2B GDP-GTP exchange factor subunit epsilon; AltName: Full=Guanine nucleotide exchange factor subunit GCD6; AltName: Full=GCD complex su [NCBI NR] gi\|171574\|gb\|AAA65498.1\| guanine nucleotide exchange factor, eIF-2B, delta subunit [Saccharomyces cerevisiae] [NCBI NR] gi\|1204152\|emb\|CAA92354.1\| Gcd6p [Saccharomyces cerevisiae] [NCBI NR] gi\|1122344\|emb\|CAA92362.1\| Gcd6p [Saccharomyces cerevisiae] [NCBI NR] Translation initiation factor eIF-2B subunit epsilon OS=Saccharomyces cerevisiae GN=GCD6 PE=1 SV=1 [Swiss-Prot] (P32501) Translation initiation factor eIF-2B epsilon subunit (eIF-2B GDP-GTP exchange factor) (Guanine nucleotide exchange factor subunit GCD6) (GCD complex subunit GCD6) [Swiss-Prot] GCD6 Translation initiation factor eIF2b epsilon, 81 kDa subunit [mips] Catalytic epsilon subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression [SGD] GCD6, Chr IV from 884719-886857 [yeast_orfs2] Chr IV from 884719-886857 [yeastorf3.fasta] GCD6 SGDID:S0002619, Chr IV from 884720-886858, Verified ORF [yeastflysequest.fasta] GCD6 SGDID:S0002619, Chr IV from 884722-886860, Verified ORF [10112005cervSGD.fasta] Catalytic epsilon subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression [Saccharomyces cerev [nrdb_11152004_Chlamydomonas] GCD6 SGDID:S000002619, Chr IV from 884722-886860, Verified ORF, "Catalytic epsilon subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identifie [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] GCD6 SGDID:S000002619, Chr IV from 884724-886862, Verified ORF, "Catalytic epsilon subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identifie [yeast-contam.fasta] GCD6 SGDID:S000002619, Chr IV from 884725-886863, Verified ORF, "Catalytic epsilon subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identifie [SGD_S-cerevisiae_na_08-19-2008_con_reversed.fasta] Translation initiation factor eIF-2B subunit epsilon (eIF-2B GDP-GTP exchange factor subunit epsilon) (Guanine nucleotide exchange factor subunit GCD6) (GCD complex subunit GCD6) [nr-271106-contam.fasta] Gcd6p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] guanine nucleotide exchange factor, eIF-2B, delta subunit [nr-271106-contam.fasta] Catalytic epsilon subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression; Gcd6p [Saccharomyce [nr-271106-contam.fasta] GCD6 SGDID:S000002619, Chr IV from 884725-886863, Verified ORF, "Catalytic epsilon subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression" [yeast-bovrabprots.fasta] GCD6 SGDID:S000002619, Chr IV from 884724-886862, Verified ORF, "Catalytic epsilon subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression" [yeast-contam_custom.fasta] [chemostatdb.fasta] Translation initiation factor eIF-2B subunit epsilon OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=GCD6 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] GCD6 SGDID:S000002619, Chr IV from 884727-886865, Genome Release 64-1-1, Verified ORF, "Catalytic epsilon subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression" [yeast-030211-contam.fasta] GCD6 SGDID:S000002619, Chr IV from 884727-886865, Genome Release 64-2-1, Verified ORF, "Catalytic epsilon subunit of the translation initiation factor eIF2B; eIF2B is the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression; forms cytoplasmic foci upon DNA replication stress" [20141208_orf_trans.fasta] YDR211W SGDID:S000002619, Chr IV from 884727-886865, Genome Release 64-1-1, Verified ORF, "Catalytic epsilon subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	712 amino acids
Gene Ontology:
Cellular Component:	guanyl-nucleotide exchange factor complex [IDA ][IPI ] eukaryotic translation initiation factor 2B complex [IDA ][IGI][IMP][IPI ]
Biological Process:	regulation of translational initiation [IDA][IGI][IPI]
Molecular Function:	translation initiation factor activity [IGI][IPI] guanyl-nucleotide exchange factor activity [IDA][IMP ]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MAGKKGQKKS GLGNHGKNSD MDVEDRLQAV VLTDSYETRF MPLTAVKPRC LLPLANVPLI  60
   61 EYTLEFLAKA GVHEVFLICS SHANQINDYI ENSKWNLPWS PFKITTIMSP EARCTGDVMR 120
  121 DLDNRGIITG DFILVSGDVL TNIDFSKMLE FHKKMHLQDK DHISTMCLSK ASTYPKTRTI 180
  181 EPAAFVLDKS TSRCIYYQDL PLPSSREKTS IQIDPELLDN VDEFVIRNDL IDCRIDICTS 240
  241 HVPLIFQENF DYQSLRTDFV KGVISSDILG KHIYAYLTDE YAVRVESWQT YDTISQDFLG 300
  301 RWCYPLVLDS NIQDDQTYSY ESRHIYKEKD VVLAQSCKIG KCTAIGSGTK IGEGTKIENS 360
  361 VIGRNCQIGE NIRIKNSFIW DDCIIGNNSI IDHSLIASNA TLGSNVRLND GCIIGFNVKI 420
  421 DDNMDLDRNT KISASPLKNA GSRMYDNESN EQFDQDLDDQ TLAVSIVGDK GVGYIYESEV 480
  481 SDDEDSSTEA CKEINTLSNQ LDELYLSDDS ISSATKKTKK RRTMSVNSIY TDREEIDSEF 540
  541 EDEDFEKEGI ATVERAMENN HDLDTALLEL NTLRMSMNVT YHEVRIATIT ALLRRVYHFI 600
  601 ATQTLGPKDA VVKVFNQWGL LFKRQAFDEE EYIDLMNIIM EKIVEQSFDK PDLILFSALV 660
  661 SLYDNDIIEE DVIYKWWDNV STDPRYDEVK KLTVKWVEWL QNADEESSSE EE

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		GCD1 GCD11 GCD2 GCD6 GCD7 GCN3 SUI2 SUI3
View Details	Hazbun TR, et al. (2003)		GCD6 NOP9 NSR1 SNU13
View Details	(MIPS) Mewes HW, et al. (2004)		GCD1 GCD2 GCD6 GCD7 GCN3
View Details	Krogan NJ, et al. (2006)		ADE1 GCD1 GCD11 GCD2 GCD6 GCD7 GCN3 MRF1 MRH4 SUI2
View Details	Gavin AC, et al. (2006)		GCD1 GCD11 GCD2 GCD6 GCD7 GCN3
View Details	Gavin AC, et al. (2002)		ASC1 CCT4 CHC1 CKA1 COG8 FKS1 GCD1 GCD11 GCD2 GCD6 GCD7 GCN3 GUT1 IST1 NAT1 NEM1 PDC1 PRO1 RRP40 RRP46 SCP160 SUI2 SUI3 SWI3 XKS1
View Details	Ho Y, et al. (2002)		CDC33 FAA4 FAL1 FET3 GCD1 GCD11 GCD2 GCD6 GCD7 GCN3 LOS1 MET18 NMD5 PRO3 SCW4 SUI2 SUI3 TIF1, TIF2 TIF4631 TIF4632 TUP1 VAC8 VMA8
View Details	Qiu et al. (2008)		GCD1 GCD2 GCD6 GCD7 GCN3

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	NOP9	No Comments	Hazbun TR, et al. (2003)
View Run	PGA1	No Comments	Hazbun TR, et al. (2003)
View Run	NOP9	No Comments	Hazbun TR, et al. (2003)
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	ZDS1	Sample 2- cpn2 from june 2005	McCusker D, et al (2007)
View Run	ZDS1	Sample 3 - cpn + atp, from june 2005	McCusker D, et al (2007)
View Run	BOI1	Sample boi 1 with ha tag from october 2005	McCusker D, et al (2007)
View Run	MLP2	#12 Mitotic Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#11 Mitotic Prep1-TiO2 enriched, new search criteria	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	PRP42	GCD6	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	GCD6	Huh WK, et al. (2003)

Protein Structure Data

[What does the above image mean?]
[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..299]	PSI-BLAST	116.917458	N-acetylglucosamine 1-phosphate uridyltransferase GlmU, C-terminal domain; N-acetylglucosamine 1-phosphate uridyltransferase GlmU, N-terminal domain
2	View Details	[300..516]	PSI-BLAST	116.917458	N-acetylglucosamine 1-phosphate uridyltransferase GlmU, C-terminal domain; N-acetylglucosamine 1-phosphate uridyltransferase GlmU, N-terminal domain
3	View Details	[517..626]	deduced	N/A	No confident structure predictions are available.
4	View Details	[627..712]	Pfam	27.60206	eIF4-gamma/eIF5/eIF2-epsilon No confident structure predictions are available.

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
uridylyltransferase activity	3.74072767898515	bayes_pls_golite062009
transferase activity, transferring hexosyl groups	2.70047942699289	bayes_pls_golite062009
UTP:glucose-1-phosphate uridylyltransferase activity	2.65167602524122	bayes_pls_golite062009
UTP-monosaccharide-1-phosphate uridylyltransferase activity	2.46432078102948	bayes_pls_golite062009
UDP-glycosyltransferase activity	2.33861720107822	bayes_pls_golite062009
transferase activity	2.17535315350752	bayes_pls_golite062009
nucleotidyltransferase activity	2.00651267140641	bayes_pls_golite062009
catalytic activity	1.88676710996045	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	1.49149681135671	bayes_pls_golite062009
acetylgalactosaminyltransferase activity	1.33788133706753	bayes_pls_golite062009
acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine O-acyltransferase activity	1.17181714897181	bayes_pls_golite062009
polypeptide N-acetylgalactosaminyltransferase activity	1.10309252319727	bayes_pls_golite062009
binding	1.09657219604879	bayes_pls_golite062009
mannosyltransferase activity	0.91123217364068	bayes_pls_golite062009
transferase activity, transferring glycosyl groups	0.897978517422275	bayes_pls_golite062009
acyltransferase activity	0.741838450922005	bayes_pls_golite062009
transferase activity, transferring acyl groups other than amino-acyl groups	0.717805296858439	bayes_pls_golite062009
transferase activity, transferring acyl groups	0.555398952276587	bayes_pls_golite062009
UDP-N-acetylglucosamine diphosphorylase activity	0.513376694534047	bayes_pls_golite062009
O-acyltransferase activity	0.319932817519902	bayes_pls_golite062009
mannose-phosphate guanylyltransferase activity	0.2363835251362	bayes_pls_golite062009
cytidylyltransferase activity	0.218759888732981	bayes_pls_golite062009
guanylyltransferase activity	0.0539392112504681	bayes_pls_golite062009

Term	Confidence	Notes
translation initiation factor activity	9.37171190530754	bayes_pls_golite062009
translation regulator activity	5.35226950142169	bayes_pls_golite062009
binding	3.0667965351474	bayes_pls_golite062009
nucleic acid binding	2.34284977509828	bayes_pls_golite062009
transcription regulator activity	1.76231475025891	bayes_pls_golite062009
protein binding	1.73688475473996	bayes_pls_golite062009
RNA cap binding	1.73309326409609	bayes_pls_golite062009
DNA binding	1.62032607987786	bayes_pls_golite062009
transcription factor activity	0.923253759652909	bayes_pls_golite062009
structural molecule activity	0.908029258648777	bayes_pls_golite062009
translation factor activity, nucleic acid binding	0.803700928110921	bayes_pls_golite062009
mRNA binding	0.46864854653143	bayes_pls_golite062009
RNA binding	0.40024158562931	bayes_pls_golite062009
transcription activator activity	0.0232727461674547	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2