Protein Overview: SPT5

General Information:
Name(s) found:	SPT5 / YML010W [SGD]
Description(s) found: Found 26 descriptions. SHOW ALL	Protein involved in regulating Pol I and Pol II transcription and pre-mRNA processing; forms a complex with Spt4p; contains a C-terminal repeat domain that is a target for phosphorylation by Sgv1p [SGD]
Organism:	Saccharomyces cerevisiae
Length:	1063 amino acids
Gene Ontology:
Cellular Component:	nucleus [IDA] transcription elongation factor complex [IPI] DSIF complex [IPI] mitochondrion [IDA ]
Biological Process:	regulation of transcription, DNA-dependent [IGI][IMP][IPI] chromatin organization [TAS] RNA elongation from RNA polymerase II promoter [IPI]
Molecular Function:	RNA polymerase II transcription elongation factor activity [IGI][IMP][IPI]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSDNSDTNVS MQDHDQQFAD PVVVPQSTDT KDENTSDKDT VDSGNVTTTE STERAESTSN  60
   61 IPPLDGEEKE AKSEPQQPED NAETAATEQV SSSNGPATDD AQATLNTDSS EANEIVKKEE 120
  121 GSDERKRPRE EDTKNSDGDT KDEGDNKDED DDEDDDDDDD DEDDDDEAPT KRRRQERNRF 180
  181 LDIEAEVSDD EDEDEDEEDS ELVREGFITH GDDEDDEASA PGARRDDRLH RQLDQDLNKT 240
  241 SEEDAQRLAK ELRERYGRSS SKQYRAAAQD GYVPQRFLLP SVDTATIWGV RCRPGKEKEL 300
  301 IRKLLKKKFN LDRAMGKKKL KILSIFQRDN YTGRIYIEAP KQSVIEKFCN GVPDIYISQK 360
  361 LLIPVQELPL LLKPNKSDDV ALEEGSYVRI KRGIYKGDLA MVDQISENNL EVMLKIVPRL 420
  421 DYGKFDEIDP TTQQRKSRRP TFAHRAPPQL FNPTMALRLD QANLYKRDDR HFTYKNEDYI 480
  481 DGYLYKSFRI QHVETKNIQP TVEELARFGS KEGAVDLTSV SQSIKKAQAA KVTFQPGDRI 540
  541 EVLNGEQRGS KGIVTRTTKD IATIKLNGFT TPLEFPISTL RKIFEPGDHV TVINGEHQGD 600
  601 AGLVLMVEQG QVTFMSTQTS REVTITANNL SKSIDTTATS SEYALHDIVE LSAKNVACII 660
  661 QAGHDIFKVI DETGKVSTIT KGSILSKINT ARARVSSVDA NGNEIKIGDT IVEKVGSRRE 720
  721 GQVLYIQTQQ IFVVSKKIVE NAGVFVVNPS NVEAVASKDN MLSNKMDLSK MNPEIISKMG 780
  781 PPSSKTFQQP IQSRGGREVA LGKTVRIRSA GYKGQLGIVK DVNGDKATVE LHSKNKHITI 840
  841 DKHKLTYYNR EGGEGITYDE LVNRRGRVPQ ARMGPSYVSA PRNMATGGIA AGAAATSSGL 900
  901 SGGMTPGWSS FDGGKTPAVN AHGGSGGGGV SSWGGASTWG GQGNGGASAW GGAGGGASAW 960
  961 GGQGTGATST WGGASAWGNK SSWGGASTWA SGGESNGAMS TWGGTGDRSA YGGASTWGGN1020
 1021 NNNKSTRDGG ASAWGNQDDG NRSAWNNQGN KSNYGGNSTW GGH

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		DST1 IWR1 PPH3 PSY2 PSY4 RPB10 RPB11 RPB2 RPB3 RPB4 RPB5 RPB7 RPB8 RPB9 RPO21 RPO26 SPT4 SPT5 SSD1 TFG1 TFG2
View Details	Krogan NJ, et al. (2006)		ABF2 ARG82 CKI1 CMR1 HHF1, HHF2 HTA1 HTA2 HTB1 HTB2 NUP188 PPH3 PSY2 PSY4 RRD1 SLX5 SLX8 SPT5 STP1 TOP2
View Details	Gavin AC, et al. (2002)		ABD1 AOS1 ASC1 ATG9 BEM2 CLU1 FUN12 GLT1 HCR1 IST2 IWR1 KTR5 LSG1 NIP1 NOG1 NOP7 PPH3 PRT1 PSY2 PSY4 RGA2 RIC1 RLI1 RPB11 RPB2 RPB3 RPB4 RPB5 RPB7 RPB8 RPB9 RPG1 RPO21 RPO26 SPT4 SPT5 SUA7 SUI1 SUI2 SUI3 SUP45 TAF14 TDH3 TFG1 TFG2 TIF34 TIF35 TIF5 TRM3 UTP6 YGL036W
View Details	Ho Y, et al. (2002)		BCY1 CAR2 CPR6 ESS1 HEF3 HSP104 HXT6 MDN1 PUP2 RPB3 RPN1 RPO21 SPT5 TFG1 TOM1 UTP22 YHR033W
View Details	Qiu et al. (2008)		CDC73 CHD1 ELA1 ELP6 IKI1 LEO1 PAF1 RTF1 SPN1 SPT16 SPT4 SPT5

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	TOR1	No Comments	Reinke A, et al. (2004)
View Run	TOR1	No Comments	Reinke A, et al. (2004)
View Run	TOR2	No Comments	Reinke A, et al. (2004)
View Run	CWC25	No Comments	Hazbun TR, et al. (2003)
View Run	LST8	No Comments	Reinke A, et al. (2004)
View Run	TOR2	No Comments	Reinke A, et al. (2004)
View Run	KOG1	No Comments	Reinke A, et al. (2004)
View Run	TOR2	No Comments	Reinke A, et al. (2004)
View Run	CLB2	No Comments	McCusker D, et al (2007)
View Run	RPA135	his-HA tag on RPA135	Schneider, DA, et al. (2006)
View Run	RPA135	his-HA tag on RPA135	Schneider, DA, et al. (2006)
View Run	DAD1	Dam1-765 complex purified from yeast with Dad1-TAP	Shimogawa MM, et al. (2006)
View Run	CEP3	sample cbf3 from feb 2005	Sandall S, et all (2006)
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	RLF2	identification data - first search in cascade	Green EM, et al (2005)
View Run	ZDS1	Sample 2- cpn2 from june 2005	McCusker D, et al (2007)
View Run	ZDS1	Sample 3 - cpn + atp, from june 2005	McCusker D, et al (2007)
View Run	BOI1	Sample boi 1 with ha tag from october 2005	McCusker D, et al (2007)
View Run	BOI1	Sample boi1 with gst from october 2005	McCusker D, et al (2007)
View Run	BOI1	Sample bob1 (2nd set) from october 2005	McCusker D, et al (2007)
View Run	BOI2	Boi 2 gst control	McCusker D, et al (2007)
View Run	MLP2	#32 Asynchronous Prep-No Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#03 Alpha Factor Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	PRP42	SPT5	1	Unpublished Fields Lab Data
View Screen	UBP6	SPT5	1	Unpublished Fields Lab Data
View Screen	TRZ1	SPT5	1	Hazbun TR, et al. (2003)
View Screen	ORC3	SPT5	1	Unpublished Fields Lab Data
View Screen	SPP382	SPT5	1	Hazbun TR, et al. (2003)

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	SPT5	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..232]	MSA	37.191997	View MSA. No confident structure predictions are available.
2	View Details	[233..314]	Pfam	13.366532	Supt5 repeat No confident structure predictions are available.
3	View Details	[315..381]	Pfam	15.09691	Supt5 repeat No confident structure predictions are available.
4	View Details	[382..440]	Pfam	5.200659	KOW motif No confident structure predictions are available.
5	View Details	[441..531]	deduced	N/A	No confident structure predictions are available.
6	View Details	[532..570]	Pfam	2.744727	KOW motif No confident structure predictions are available.
7	View Details	[571..617]	Pfam	4.0	KOW motif No confident structure predictions are available.
8	View Details	[618..794]	deduced	N/A	No confident structure predictions are available.
9	View Details	[795..842]	Pfam	5.0	KOW motif No confident structure predictions are available.
10	View Details	[843..908]	deduced	N/A	No confident structure predictions are available.
11	View Details	[909..1063]	PSI-BLAST	3.69897	Fibrinogen

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.50

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2