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Bait Protein: LST8
Organism: Saccharomyces cerevisiae
Publication: Reinke A, Anderson S, McCaffery JM, Yates J 3rd, Aronova S, Chu S, Fairclough S, Iverson C, Wedaman KP, Powers T. TOR complex 1 includes a novel component, Tco89p (YPL180w), and cooperates with Ssd1p to maintain cellular integrity in Saccharomyces cerevisiae. J Biol Chem. 2004 Apr 9;279(15):14752-62. Epub 2004 Jan 21.
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  Hit Protein Protein Desc Sequence Count Spectrum Count Sequence Coverage NSAF Mol. Wt.
[View Peptides]   CMD1 Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, act... 8 18 68.0% 0.0137 16135
[View Peptides]   SUP35 Translation termination factor eRF3; altered protein conformation creates the [PSI(+)] prion, a domi... 51 107 66.1% 0.0175 76551
[View Peptides]   ENO2 Enolase II, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosp... 19 25 61.1% 0.00642 46914
[View Peptides]   DEF1 RNAPII degradation factor, forms a complex with Rad26p in chromatin, enables ubiquitination and prot... 76 282 57.0% 0.0429 83973
[View Peptides]   TOR2 PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth i... 135 328 56.4% 0.0149 281509
[View Peptides]   RPP2B Ribosomal protein P2 beta, a component of the ribosomal stalk, which is involved in the interaction ... 6 6 53.6% 0.00612 11050
[View Peptides]   TOR1 PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that controls growth in... 123 241 53.3% 0.0109 281138
[View Peptides]   AVO2 Component of a complex containing the Tor2p kinase and other proteins, which may have a role in regu... 25 30 53.3% 0.0079 47140
[View Peptides]   SSE1 ATPase that is a component of the heat shock protein Hsp90 chaperone complex; binds unfolded protein... 35 80 51.9% 0.0129 77367
[View Peptides]   BIT61 Subunit of TORC2 (Tor2p-Lst8p-Avo1-Avo2-Tsc11p-Bit61p-Slm1p-Slm2p), a membrane-associated complex th... 21 31 51.4% 0.0064 60841
[View Peptides]   LST8 Protein required for the transport of amino acid permease Gap1p from the Golgi to the cell surface; ... 20 48 50.5% 0.0178 34034
[View Peptides]   IBI2 Protein of unknown function that associates with ribosomes; green fluorescent protein (GFP)-fusion p... 7 43 48.7% 0.0245 22179
[View Peptides]   TSC11 Subunit of TORC2 (Tor2p-Lst8p-Avo1-Avo2-Tsc11p-Bit61p), a membrane-associated complex that regulates... 55 113 43.8% 0.00886 164368
[View Peptides]   YPR137C-A, YAR010C Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; ... 15 26 41.6% 0.00663 49072
[View Peptides]   YGR038C-A Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; ... 15 26 41.6% 0.00663 49034
[View Peptides]   YGR027W-A Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; ... 15 26 41.6% 0.00663 49056
[View Peptides]   YER159C-A, YDR31... Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; ... 15 26 41.6% 0.00663 49129
[View Peptides]   YDR365W-A, YPL25... Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; ... 15 26 41.6% 0.00663 48953
[View Peptides]   YDR365W-A, YPL25... Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; ... 15 26 41.6% 0.00663 48953
[View Peptides]   YER159C-A, YDR31... Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; ... 15 26 41.6% 0.00663 49129
[View Peptides]   YDR261C-C Retrotransposon TYA Gag gene co-transcribed with TYB Pol; in YDRCTY1-3 TYB is mutant and probably no... 15 26 41.6% 0.00663 49009
[View Peptides]   YDR098C-A Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; ... 15 26 41.6% 0.00663 49057
[View Peptides]   SPT5 Protein involved in regulating Pol I and Pol II transcription and pre-mRNA processing; forms a compl... 48 111 40.2% 0.0117 115650
[View Peptides]   PDC1 Major of three pyruvate decarboxylase isozymes, key enzyme in alcoholic fermentation, decarboxylates... 17 28 37.3% 0.00558 61495
[View Peptides]   FKS1 Catalytic subunit of 1,3-beta-D-glucan synthase, functionally redundant with alternate catalytic sub... 55 111 36.8% 0.00664 214849
[View Peptides]   AVO1 Component of a membrane-bound complex containing the Tor2p kinase and other proteins, which may have... 38 63 35.6% 0.00601 131378
[View Peptides]   TCO89 Subunit of TORC1 (Tor1p or Tor2p-Kog1p-Lst8p-Tco89p), a complex that regulates growth in response to... 27 35 35.5% 0.00491 88845
[View Peptides]   KOG1 Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or To... 46 77 34.6% 0.00555 177608
[View Peptides]   FBA1 Fructose 1,6-bisphosphate aldolase, required for glycolysis and gluconeogenesis; catalyzes conversio... 8 12 33.1% 0.00375 39621
[View Peptides]   PGK1 3-phosphoglycerate kinase, catalyzes transfer of high-energy phosphoryl groups from the acyl phospha... 8 11 32.5% 0.00297 44738
[View Peptides]   SSC1 Hsp70 family ATPase, constituent of the import motor component of the Translocase of the Inner Mitoc... 19 36 32.3% 0.00617 70628
[View Peptides]   YDJ1 Protein chaperone involved in regulation of the HSP90 and HSP70 functions; involved in protein trans... 14 19 30.8% 0.00521 44671
[View Peptides]   HSP60 Tetradecameric mitochondrial chaperonin required for ATP-dependent folding of precursor polypeptides... 12 17 29.7% 0.00333 60752
[View Peptides]   SPT4 Protein involved in the regulating Pol I and Pol II transcription, pre-mRNA processing, kinetochore ... 3 4 29.4% 0.0044 11158
[View Peptides]   ACT1 Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functio... 5 6 28.8% 0.00179 41690
[View Peptides]   RPP2A Ribosomal protein P2 alpha, a component of the ribosomal stalk, which is involved in the interaction... 2 3 28.3% 0.00317 10746
[View Peptides]   GPM1 Tetrameric phosphoglycerate mutase, mediates the conversion of 3-phosphoglycerate to 2-phosphoglycer... 5 8 27.9% 0.00363 27609
[View Peptides]   CDC19 Pyruvate kinase, functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvat... 9 12 26.0% 0.00269 54545
[View Peptides]   HYP2 Translation elongation factor eIF-5A, previously thought to function in translation initiation; simi... 3 3 25.5% 0.00214 17114
[View Peptides]   PET10 Protein of unknown function that co-purifies with lipid particles; expression pattern suggests a rol... 4 4 23.3% 0.00159 31246
[View Peptides]   YHB1 Nitric oxide oxidoreductase, flavohemoglobin involved in nitric oxide detoxification; plays a role i... 4 4 21.8% 0.00112 44646
[View Peptides]   GSC2 Catalytic subunit of 1,3-beta-glucan synthase, involved in formation of the inner layer of the spore... 30 54 21.4% 0.0032 216988
[View Peptides]   PHO84 High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated... 8 8 21.0% 0.00153 64382
[View Peptides]   VMA2 Subunit B of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), a... 6 6 20.3% 0.0013 57749
[View Peptides]   SAR1 GTPase, GTP-binding protein of the ARF family, component of COPII coat of vesicles; required for tra... 2 2 20.0% 0.00118 21451
[View Peptides]   KAR2 ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding i... 16 26 19.9% 0.00428 74468
[View Peptides]   KAR2 ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding i... 16 26 19.9% 0.00428 74468
[View Peptides]   TEF1, TEF2 Translational elongation factor EF-1 alpha; also encoded by TEF2; functions in the binding reaction ... 5 6 19.9% 0.00147 50033
[View Peptides]   TEF1, TEF2 Translational elongation factor EF-1 alpha; also encoded by TEF2; functions in the binding reaction ... 5 6 19.9% 0.00147 50033
[View Peptides]   GVP36 BAR domain-containing protein that localizes to both early and late Golgi vesicles; required for ada... 4 6 19.0% 0.00206 36670
[View Peptides]   SCJ1 One of several homologs of bacterial chaperone DnaJ, located in the ER lumen where it cooperates wit... 4 4 18.8% 0.00111 44800
[View Peptides]   RPP0 Conserved ribosomal protein P0 similar to rat P0, human P0, and E. coli L10e; shown to be phosphoryl... 4 7 18.6% 0.00252 33717
[View Peptides]   RNQ1 [PIN(+)] prion, an infectious protein conformation that is generally an ordered protein aggregate 4 4 18.5% 0.00111 42580
[View Peptides]   PIM1 ATP-dependent Lon protease, involved in degradation of misfolded proteins in mitochondria; required ... 13 25 18.4% 0.00247 127112
[View Peptides]   ASC1 G-protein beta subunit and guanine nucleotide dissociation inhibitor for Gpa2p; ortholog of RACK1 th... 2 2 17.6% 7.03E-4 34805
[View Peptides]   PMA1 Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plas... 10 16 17.5% 0.00195 99619
[View Peptides]   YJL206C Putative protein of unknown function; similar to transcriptional regulators from the Zn[2]-Cys[6] bi... 9 10 17.0% 0.00148 86663
[View Peptides]   MDJ1 Co-chaperone that stimulates the ATPase activity of the HSP70 protein Ssc1p; involved in protein fol... 5 6 17.0% 0.00132 55561
[View Peptides]   ADH1 Alcohol dehydrogenase, fermentative isozyme active as homo- or heterotetramers; required for the red... 5 8 16.7% 0.00258 36849
[View Peptides]   HSC82 Cytoplasmic chaperone of the Hsp90 family, redundant in function and nearly identical with Hsp82p, a... 7 10 15.2% 0.00159 80900
[View Peptides]   CCT8 Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of... 5 5 15.1% 9.87E-4 61662
[View Peptides]   MLC1 Essential light chain for Myo1p, light chain for Myo2p; stabilizes Myo2p by binding to the neck regi... 2 4 14.8% 0.00301 16444
[View Peptides]   EFT2, EFT1 Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein s... 6 6 12.9% 7.99E-4 93289
[View Peptides]   EFT2, EFT1 Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein s... 6 6 12.9% 7.99E-4 93289
[View Peptides]   CCT2 Subunit beta of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assemb... 4 5 12.9% 0.00106 57203
[View Peptides]   YDR261C-D Retrotransposon TYA Gag and TYB Pol genes; in YDRCTY1-3 TYB is mutant and probably non-functional 15 26 12.3% 0.00182 174779
[View Peptides]   TCP1 Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; inv... 3 3 12.0% 6.02E-4 60481
[View Peptides]   CCT4 Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of... 3 5 11.4% 0.00106 57604
[View Peptides]   YBL081W Non-essential protein of unknown function; null mutation results in a decrease in plasma membrane el... 2 2 11.4% 6.09E-4 40767
[View Peptides]   YGR038C-B Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 15 26 11.3% 0.00166 190737
[View Peptides]   YER138C Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 15 26 11.3% 0.00166 190671
[View Peptides]   YDR365W-B Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 15 26 11.3% 0.00166 190643
[View Peptides]   YDR316W-B Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 15 26 11.3% 0.00166 191603
[View Peptides]   YDR098C-B Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 15 26 11.3% 0.00166 191131
[View Peptides]   YGR027W-B Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 15 26 11.2% 0.00166 192088
[View Peptides]   YER160C Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 15 26 11.2% 0.00166 192176
[View Peptides]   BIT2 Subunit of TORC2, a membrane-associated complex that regulates actin cytoskeletal dynamics during po... 4 4 11.2% 8.23E-4 61302
[View Peptides]   LAT1 Dihydrolipoamide acetyltransferase component (E2) of pyruvate dehydrogenase complex, which catalyzes... 3 3 11.0% 6.98E-4 51818
[View Peptides]   CCT6 Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, essential protein that is re... 3 3 10.8% 6.16E-4 59924
[View Peptides]   NRP1 Putative RNA binding protein of unknown function; localizes to stress granules induced by glucose de... 4 5 10.4% 7.8E-4 79299
[View Peptides]   TIF34 eIF3i subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for ... 2 2 10.4% 6.46E-4 38755
[View Peptides]   SNC2 Vesicle membrane receptor protein (v-SNARE) involved in the fusion between Golgi-derived secretory v... 2 2 10.4% 0.00195 12957
[View Peptides]   ECM10 Heat shock protein of the Hsp70 family, localized in mitochondrial nucleoids, plays a role in protei... 7 19 10.1% 0.00331 70085
[View Peptides]   NSR1 Nucleolar protein that binds nuclear localization sequences, required for pre-rRNA processing and ri... 2 2 9.9% 5.42E-4 44535
[View Peptides]   VPS13 Protein of unknown function; heterooligomeric or homooligomeric complex; peripherally associated wit... 17 21 9.0% 7.49E-4 357850
[View Peptides]   PSA1 GDP-mannose pyrophosphorylase (mannose-1-phosphate guanyltransferase), synthesizes GDP-mannose from ... 2 2 8.6% 6.21E-4 39566
[View Peptides]   TIF1, TIF2 Translation initiation factor eIF4A, identical to Tif2p; DEA(D/H)-box RNA helicase that couples ATPa... 2 3 7.8% 8.52E-4 44697
[View Peptides]   TIF1, TIF2 Translation initiation factor eIF4A, identical to Tif2p; DEA(D/H)-box RNA helicase that couples ATPa... 2 3 7.8% 8.52E-4 44697
[View Peptides]   LEM3 Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid... 3 3 7.7% 8.13E-4 47438
[View Peptides]   NCE102 Protein of unknown function; contains transmembrane domains; involved in secretion of proteins that ... 2 2 7.5% 0.0013 18967
[View Peptides]   SAH1 S-adenosyl-L-homocysteine hydrolase, catabolizes S-adenosyl-L-homocysteine which is formed after don... 2 2 7.3% 5.0E-4 49126
[View Peptides]   DED1 ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all ye... 2 2 7.1% 3.71E-4 65553
[View Peptides]   NHX1 Endosomal Na+/H+ exchanger, required for intracellular sequestration of Na+; required for osmotolera... 2 2 7.0% 3.54E-4 70148
[View Peptides]   NOP58 Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the ... 2 2 6.8% 4.39E-4 56956
[View Peptides]   NOP56 Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that ... 2 2 6.7% 4.45E-4 56864
[View Peptides]   DNF1 Aminophospholipid translocase (flippase) that localizes primarily to the plasma membrane; contribute... 6 8 6.6% 5.71E-4 177797
[View Peptides]   KRE6 Protein required for beta-1,6 glucan biosynthesis; putative beta-glucan synthase; appears functional... 3 3 6.4% 4.67E-4 80123
[View Peptides]   RNR1 One of two large regulatory subunits of ribonucleotide-diphosphate reductase; the RNR complex cataly... 3 3 6.0% 3.79E-4 99561
[View Peptides]   SPA2 Component of the polarisome, which functions in actin cytoskeletal organization during polarized gro... 4 4 5.7% 3.06E-4 163142
[View Peptides]   CRT10 Protein involved in transcriptional regulation of RNR2 and RNR3; expression of the gene is induced b... 3 3 5.6% 3.52E-4 109715
[View Peptides]   CCT7 Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of... 2 2 5.6% 4.08E-4 59736
[View Peptides]   SAM3 High-affinity S-adenosylmethionine permease, required for utilization of S-adenosylmethionine as a s... 2 3 5.5% 5.73E-4 64354
[View Peptides]   PGI1 Glycolytic enzyme phosphoglucose isomerase, catalyzes the interconversion of glucose-6-phosphate and... 2 2 5.4% 4.05E-4 61299
[View Peptides]   ERB1 Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Ytm1p that is required fo... 2 2 5.3% 2.78E-4 91705
[View Peptides]   UBI4 Ubiquitin, becomes conjugated to proteins, marking them for selective degradation via the ubiquitin-... 2 10 4.7% 0.00294 42826
[View Peptides]   MRPL10 Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL10 and YmL18)... 2 2 4.7% 6.97E-4 36347
[View Peptides]   MMS1 Subunit of an E3 ubiquitin ligase complex involved in resolving replication intermediates or prevent... 4 4 4.5% 3.19E-4 161237
[View Peptides]   KEL1 Protein required for proper cell fusion and cell morphology; functions in a complex with Kel2p to ne... 3 3 4.5% 2.89E-4 131094
[View Peptides]   YEF3 Translational elongation factor 3, stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes b... 2 2 4.0% 2.15E-4 115945
[View Peptides]   YLR035C-A Retrotransposon TYA Gag and TYB Pol genes; polyprotein is processed to make a nucleocapsid-like prot... 3 4 3.9% 3.88E-4 131617
[View Peptides]   YAR009C Retrotransposon TYA Gag and TYB Pol genes; in YARCTY1-1 TYB is mutant and probably non-functional 3 4 3.8% 3.75E-4 136732
[View Peptides]   BDF1 Protein involved in transcription initiation at TATA-containing promoters; associates with the basal... 2 2 3.8% 3.27E-4 76978
[View Peptides]   TFP1 Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein prec... 2 4 3.5% 4.19E-4 118637
[View Peptides]   DNF2 Aminophospholipid translocase (flippase) that localizes primarily to the plasma membrane; contribute... 3 4 3.3% 2.78E-4 182617
[View Peptides]   DRS2 Aminophospholipid translocase (flippase) that maintains membrane lipid asymmetry in post-Golgi secre... 2 2 3.2% 1.66E-4 153844
[View Peptides]   YEL025C Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to bo... 2 2 3.0% 1.89E-4 136069
[View Peptides]   INO80 ATPase, subunit of a complex containing actin and several actin-related proteins that has chromatin ... 2 4 2.5% 3.01E-4 171454
[View Peptides]   FAS2 Alpha subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty ... 2 2 2.4% 1.19E-4 206945
[View Peptides]   VPS15 Myristoylated serine/threonine protein kinase involved in vacuolar protein sorting; functions as a m... 2 2 2.4% 1.54E-4 166358
[View Peptides]   RPO21 RNA polymerase II largest subunit B220, part of central core; phosphorylation of C-terminal heptapep... 2 2 2.0% 1.29E-4 191610
[View Peptides]   KEM1 Evolutionarily-conserved 5'-3' exonuclease component of cytoplasmic processing (P) bodies involved i... 2 2 2.0% 1.47E-4 175459
[View Peptides]   NET1 Core subunit of the RENT complex, which is a complex involved in nucleolar silencing and telophase e... 2 2 1.7% 1.89E-4 128531
[View Peptides]   CHC1 Clathrin heavy chain, subunit of the major coat protein involved in intracellular protein transport ... 2 2 1.3% 1.36E-4 187233
[View Peptides]   SNF2 Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation... 2 4 1.1% 2.63E-4 194050

YRC Informatics Platform - Version 3.0
Created and Maintained by: Michael Riffle