Protein Overview: SNF12

General Information:
Name(s) found:	SNF12 / YNR023W [SGD]
Description(s) found: SHOW ONLY BEST	pir\|\|S19063 hypothetical protein YNR023w precursor, mitochondrial - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|6324350\|ref\|NP_014420.1\| 73 kDa subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; homolog of Rsc6p subunit of the RSC chromatin remodeling complex; deletion mutants are temperature-sensitive; Snf12p [Saccharomy [NCBI NR] gi\|4107\|emb\|CAA44296.1\| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR] gi\|1711619\|sp\|P53628.1\|SNF12_YEAST RecName: Full=Transcription regulatory protein SNF12; AltName: Full=SWI/SNF complex component SWP73 [NCBI NR] gi\|1302512\|emb\|CAA96302.1\| SNF12 [Saccharomyces cerevisiae] [NCBI NR] (P53628) Transcription regulatory protein SNF12 (SWI/SNF complex component SWP73) [Swiss-Prot] Transcription regulatory protein SNF12 OS=Saccharomyces cerevisiae GN=SNF12 PE=1 SV=1 [Swiss-Prot] SNF12 Component of SWI/SNF global transcription activator complex [mips] 73 kDa subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; homolog of Rsc6p subunit of the RSC chromatin remodeling complex; deletion mutants are temperature-sensitive [SGD] Chr XIV from 670415-672115 [hmr_yeast5_DM3-1_pombe.fasta] SNF12, Chr XIV from 670415-672115 [yeast_orfs2] SNF12 SGDID:S0005306, Chr XIV from 670417-672117, Verified ORF [yeastflysequest.fasta] SNF12 SGDID:S0005306, Chr XIV from 670418-672118, Verified ORF [10112005cervSGD.fasta] 73 kDa subunit of the SWI/SNF transcription activation complex, homolog of Rsc6p subunit of the RSC chromatin remodeling complex [Saccharomyces cerevisiae]gi\|4107\|emb\|CAA44296.1\| unnamed protein product [Saccharomyces cerevisiae]gi\|1302512\|emb\|CAA96302. [nrdb_11152004_Chlamydomonas] SNF12 SGDID:S000005306, Chr XIV from 670420-672120, Verified ORF, "73 kDa subunit of the 11-subunit SWI/SNF chromatin remodeling complex involved in transcriptional regulation; homolog of Rsc6p subunit of the RSC chromatin remodeling complex; deletion mut [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] SNF12 SGDID:S000005306, Chr XIV from 670420-672120, Verified ORF, "73 kDa subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; homolog of Rsc6p subunit of the RSC chromatin remodeling complex; deletion mutants are te [yeast-contam.fasta] unnamed protein product [Saccharomyces cerevisiae] [nr-271106-contam.fasta] SNF12 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Transcription regulatory protein SNF12 (SWI/SNF complex component SWP73) [nr-271106-contam.fasta] 73 kDa subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; homolog of Rsc6p subunit of the RSC chromatin remodeling complex; deletion mutants are temperature-sensitive; Snf12p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] SNF12 SGDID:S000005306, Chr XIV from 670421-672121, Verified ORF, "73 kDa subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; homolog of Rsc6p subunit of the RSC chromatin remodeling complex; deletion mutants are te [SGD_S-cerevisiae_na_01-05-2010_reversed.fasta] SNF12 SGDID:S000005306, Chr XIV from 670420-672120, Verified ORF, "73 kDa subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; homolog of Rsc6p subunit of the RSC chromatin remodeling complex; deletion mutants are temperature-sensitive" [yeast-bovrabprots.fasta] [chemostatdb.fasta] SNF12 SGDID:S000005306, Chr XIV from 670421-672121, Verified ORF, "73 kDa subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; homolog of Rsc6p subunit of the RSC chromatin remodeling complex; deletion mutants are temperature-sensitive" [SGDyeast.20101221.fasta] Transcription regulatory protein SNF12 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=SNF12 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] SNF12 SGDID:S000005306, Chr XIV from 670418-672118, Genome Release 64-1-1, Verified ORF, "73 kDa subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; homolog of Rsc6p subunit of the RSC chromatin remodeling complex; deletion mutants are temperature-sensitive" [yeast-030211-contam.fasta] SNF12 SGDID:S000005306, Chr XIV from 670418-672118, Genome Release 64-2-1, Verified ORF, "73 kDa subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; relocates to the cytosol under hypoxic conditions; deletion mutants are temperature-sensitive; SNF12 has a paralog, RSC6, that arose from the whole genome duplication" [20141208_orf_trans.fasta] YNR023W SGDID:S000005306, Chr XIV from 670418-672118, Genome Release 64-1-1, Verified ORF, "73 kDa subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; homolog of Rsc6p subunit of the RSC chromatin remodeling complex; deletion mutants are temperature-sensitive" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	566 amino acids
Gene Ontology:
Cellular Component:	SWI/SNF complex [TAS] chromatin remodeling complex [TAS]
Biological Process:	chromatin remodeling [TAS]
Molecular Function:	general RNA polymerase II transcription factor activity [TAS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSKVMKPSNG KGSRKSSKAA TPDTKNFFHA KKKDPVNQDK ANNASQITPT VPHSHPSDMV  60
   61 IPDHLAELIP ELYSFQQLVD SEKRLDHFIH LRNLHMKRMV AQWERSKLSQ EFLYPHLNFP 120
  121 NVKFLRIFIS NVSENQPWQM DTNNEADLMA LENATWTMRI EGRLLDNVQA NDPAREKFSS 180
  181 FIESIVVDFK NKENDNVPST KFNAAPEENA TEGPSDKKLN LNLPLQFSLP NGDNSTTTNT 240
  241 DQNNATMGEE TAKKDMSSTT PKLESVKWQY DPNNPVDFDG LDIKRVGSEN VECTISILRK 300
  301 SSPEEPFMSY SPQLTAIIGL KSGTSHDAIF SIYKYIHLNE LLTNDESAFE NLMGNRNNHN 360
  361 SNTSTSKMLD AASSQVSIVK LDTQLITLLP SSLKESSPDT MKLTDLLSLI NSTHLLPLQP 420
  421 IEIDYTVRVD KASTYGELVL DIEVPDVNAL KFNNTQRESQ IGAAELNENA RELEQIKPKI 480
  481 ALQDKEITSV LSNLHESNKR YRFFKKISED PVKALNECIA STSNALKVLS GDEGYNEDMV 540
  541 RRANFYKENE AMLRENIEVI LSNGRM

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		ARP7 ARP9 SNF11 SNF12 SNF2 SNF5 SNF6 SWI1 SWI3 SWP82
View Details	(MIPS) Mewes HW, et al. (2004)		ARP7 ARP9 SNF11 SNF12 SNF2 SNF5 SNF6 SWI1 SWI3 TAF14
View Details	Krogan NJ, et al. (2006)		SNF12 SNF2 SNF5 SNF6 SWI1 SWI3 SWP82
View Details	Gavin AC, et al. (2006)		ARP9 SNF11 SNF12 SNF5 SNF6 SWI1 SWI3
View Details	Gavin AC, et al. (2002)		ARP9 BEM2 DIM1 ENP1 HRR25 LTV1 NOB1 PNO1 RIO2 RRP12 SNF12 SNF2 SNF5 SNF6 SWI1 SWI3 SWP82 TSR1 VPS1 YGR054W
View Details	Qiu et al. (2008)		ARP7 ARP9 SNF11 SNF12 SNF2 SNF5 SNF6 SWI1 SWI3 SWP82 TAF14

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	ZDS1	Sample 2- cpn2 from june 2005	McCusker D, et al (2007)
View Run	ZDS1	Sample 3 - cpn + atp, from june 2005	McCusker D, et al (2007)
View Run	MLP2	#13 Mitotic Prep2-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	RPC19	SNF12	1	Unpublished Fields Lab Data
View Screen	PEX6	SNF12	1	Unpublished Fields Lab Data
View Screen	CLB2	SNF12	1	Unpublished Fields Lab Data
View Screen	RSR1	SNF12	1	Unpublished Fields Lab Data
View Screen	IRE1	SNF12	1	Unpublished Fields Lab Data
View Screen	YPT35	SNF12	1	Unpublished Fields Lab Data
View Screen	PRP40	SNF12	1	Unpublished Fields Lab Data
View Screen	SPT21	SNF12	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	SNF12	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..204]	MSA	1.012974	View MSA. No confident structure predictions are available.
2	View Details	[205..263]	deduced	N/A	No confident structure predictions are available.
3	View Details	[264..566]	MSA	5.016935	View MSA. No confident structure predictions are available.
4	View Details	[305..450]	PSI-BLAST	22.0	Solution structure of the SWIB domain of mouse BRG1-associated factor 60a
5	View Details	[451..566]	MSA	1.039978	View MSA. No confident structure predictions are available.

Functions predicted (by domain):

Gene Ontology predictions

No functions predicted.

Term	Confidence	Notes
transcription regulator activity	4.01592136607858	bayes_pls_golite062009
basal transcription repressor activity	3.09418413549897	bayes_pls_golite062009
binding	2.67987108623459	bayes_pls_golite062009
transcription coactivator activity	2.58234407051051	bayes_pls_golite062009
general RNA polymerase II transcription factor activity	2.32480271621458	bayes_pls_golite062009
transcription repressor activity	1.98538788872171	bayes_pls_golite062009
transcription activator activity	1.83085389017135	bayes_pls_golite062009
transcription factor binding	1.73032563380905	bayes_pls_golite062009
transcription cofactor activity	1.60414428986898	bayes_pls_golite062009
protein binding	1.58138506346812	bayes_pls_golite062009
structural molecule activity	1.28917299936669	bayes_pls_golite062009
RNA polymerase II transcription factor activity	1.06026105710513	bayes_pls_golite062009
catalytic activity	0.121624777548864	bayes_pls_golite062009

Term	Confidence	Notes
transcription regulator activity	4.01592136607858	bayes_pls_golite062009
basal transcription repressor activity	3.09418413549897	bayes_pls_golite062009
binding	2.67987108623459	bayes_pls_golite062009
transcription coactivator activity	2.58234407051051	bayes_pls_golite062009
general RNA polymerase II transcription factor activity	2.32480271621458	bayes_pls_golite062009
transcription repressor activity	1.98538788872171	bayes_pls_golite062009
transcription activator activity	1.83085389017135	bayes_pls_golite062009
transcription factor binding	1.73032563380905	bayes_pls_golite062009
transcription cofactor activity	1.60414428986898	bayes_pls_golite062009
protein binding	1.58138506346812	bayes_pls_golite062009
structural molecule activity	1.28917299936669	bayes_pls_golite062009
RNA polymerase II transcription factor activity	1.06026105710513	bayes_pls_golite062009
catalytic activity	0.121624777548864	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2