Protein Overview: NAM9

General Information:
Name(s) found:	NAM9 / YNL137C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|854500\|emb\|CAA86888.1\| mitochondrial ribosomal protein (putative) [Saccharomyces cerevisiae] [NCBI NR] gi\|6324192\|ref\|NP_014262.1\| Mitochondrial ribosomal component of the small subunit; Nam9p [Saccharomyces cerevisiae] [NCBI NR] gi\|507139\|gb\|AAA19439.1\| NAM9+ protein [Saccharomyces cerevisiae] [NCBI NR] gi\|1346661\|sp\|P27929.2\|NAM9_YEAST RecName: Full=37S ribosomal protein NAM9, mitochondrial; AltName: Full=Nuclear accommodation of mitochondria protein 9; Flags: Precursor [NCBI NR] gi\|1302082\|emb\|CAA96019.1\| NAM9 [Saccharomyces cerevisiae] [NCBI NR] pir\|\|S55146 probable ribosomal protein S4 precursor, mitochondrial - yeast (Saccharomyces cerevisiae) [NCBI NR] 37S ribosomal protein NAM9, mitochondrial OS=Saccharomyces cerevisiae GN=NAM9 PE=1 SV=2 [Swiss-Prot] NAM9 Mitochondrial ribosomal protein, small subunit [mips] Mitochondrial ribosomal component of the small subunit [SGD] NAM9, Chr XIV from 368592-370052, reverse complement [yeast_orfs2] Chr XIV from 368592-370052, reverse complement [yeastorf3.fasta] Chr XIV from 370052-368592, reverse complement [yeastorf5.fasta] (P27929) NAM9 protein, mitochondrial precursor [4932.FASTACCERV.txt] NAM9 SGDID:S0005081, Chr XIV from 370055-368595, reverse complement, Verified ORF [yeastflysequest.fasta] Mitochondrial ribosomal protein of the small subunit; can mutate to suppress nonsense mutations in mitochondrial genes [Saccharomyces cerevisiae]gi\|854500\|emb\|CAA86888.1\| mitochondrial ribosomal protein (putative) [Saccharomyces cerevisiae]gi\|1302082\|em [nrdb_11152004_Chlamydomonas] NAM9 SGDID:S000005081, Chr XIV from 370057-368597, reverse complement, Verified ORF, "Mitochondrial ribosomal component of the small subunit " [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] NAM9 SGDID:S000005081, Chr XIV from 370057-368597, reverse complement, Verified ORF, "Mitochondrial ribosomal component of the small subunit" [SGD_S-cerevisiae_na_08-19-2008_con_reversed.fasta] mitochondrial ribosomal protein (putative) [Saccharomyces cerevisiae] [nr-271106-contam.fasta] NAM9 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Protein NAM9, mitochondrial precursor [nr-271106-contam.fasta] Mitochondrial ribosomal component of the small subunit; Nam9p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] NAM9+ protein [nr-271106-contam.fasta] [chemostatdb.fasta] 37S ribosomal protein NAM9, mitochondrial OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=NAM9 PE=1 SV=2 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] NAM9 SGDID:S000005081, Chr XIV from 370055-368595, Genome Release 64-1-1, reverse complement, Verified ORF, "Mitochondrial ribosomal component of the small subunit" [yeast-030211-contam.fasta] NAM9 SGDID:S000005081, Chr XIV from 370055-368595, Genome Release 64-2-1, reverse complement, Verified ORF, "Mitochondrial ribosomal component of the small subunit" [20141208_orf_trans.fasta] YNL137C SGDID:S000005081, Chr XIV from 370055-368595, Genome Release 64-1-1, reverse complement, Verified ORF, "Mitochondrial ribosomal component of the small subunit" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	486 amino acids
Gene Ontology:
Cellular Component:	mitochondrion [IDA] mitochondrial small ribosomal subunit [IDA][TAS]
Biological Process:	translation [IDA][TAS]
Molecular Function:	structural constituent of ribosome [IDA][TAS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MPRKANLLKS LARGRVRTSF NKYNLFNLYK KGGVDLKSKS LYQQKWTAKQ ETRAYHGEHL  60
   61 TEKRWQTVFK PKLDSVAQLD ASLRGGEIKE TPFLLQTFAV LEKRLDFALF RAMFASSVRQ 120
  121 ARQFILHGNV RVNGVKIKHP SYTLKPGDMF SVKPDKVLEA LGAKKPSFQE ALKIDKTQIV 180
  181 LWNKYVKEAK TEPKEVWEKK LENFEKMSDS NPKKLQFQEF LRQYNKNLES QQYNALKGCT 240
  241 QEGILRKLLN VEKEIGKSNN EPLSIDELKQ GLPEIQDSQL LESLNNAYQE FFKSGEIRRE 300
  301 IISKCQPDEL ISLATEMMNP NETTKKELSD GAKSALRSGK RIIAESVKLW TKNITDHFKT 360
  361 RMSDISDGSL TFDPKWAKNL KYHDPIKLSE LEGDEPKARK LINLPWQKNY VYGRQDPKKP 420
  421 FFTPWKPRPF LSPFAILPHH LEISFKTCHA VYLRDPVARP GQSEVISPFD VPVHERAYMY 480
  481 YLRNGK

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		EHD3 MRP1 MRP13 MRP17 MRP21 MRP4 MRP51 MRPS16 MRPS17 MRPS18 MRPS28 MRPS35 MRPS5 MRPS8 MRPS9 NAM9 PET123 RSM10 RSM18 RSM19 RSM22 RSM23 RSM24 RSM25 RSM26 RSM27 RSM7 SWS2
View Details	(MIPS) Mewes HW, et al. (2004)		IMG1 MRP1 MRP10 MRP13 MRP17 MRP2 MRP21 MRP4 MRP51 MRP8 MRPS16 MRPS17 MRPS18 MRPS28 MRPS35 MRPS5 MRPS8 MRPS9 NAM9 PET123 PPE1 RSM10 RSM18 RSM19 RSM22 RSM23 RSM24 RSM25 RSM26 RSM27 RSM7 VAR1 YMR31
View Details	Krogan NJ, et al. (2006)		CBF1 FYV4 MAM33 MRP1 MRP10 MRP17 MRP21 MRP4 MRP51 MRPL8 MRPS12 MRPS16 MRPS17 MRPS18 MRPS28 MRPS35 MRPS5 MRPS9 NAM9 NCL1 PET123 RIB4 RSM10 RSM18 RSM19 RSM22 RSM23 RSM24 RSM25 RSM26 RSM27 RSM28 RSM7 SVL3 SWS2 YDR124W YJR096W YKL151C YSP1
View Details	Gavin AC, et al. (2006)		EHD3 MRP1 MRP13 MRP17 MRP21 MRP4 MRP51 MRPS16 MRPS17 MRPS18 MRPS28 MRPS5 MRPS8 MRPS9 NAM9 PET123 RSM10 RSM19 RSM22 RSM23 RSM25 RSM27 RSM7
View Details	Gavin AC, et al. (2002)		BEM2 CCM1 ECM16 EHD3 FYV4 GEP3 HSC82 MRP1 MRP10 MRP13 MRP17 MRP21 MRP4 MRP51 MRPS16 MRPS17 MRPS18 MRPS28 MRPS5 MRPS8 MRPS9 NAM9 PAP2 PET123 PSD2 PUS7 RIX1 RSM10 RSM19 RSM22 RSM23 RSM24 RSM25 RSM26 RSM27 RSM7 TIF4631 UBP10 UTP20 UTP22
View Details	Qiu et al. (2008)		EHD3 IMG1 IMG2 MAM33 MHR1 MNP1 MRP1 MRP10 MRP13 MRP17 MRP2 MRP20 MRP21 MRP4 MRP49 MRP51 MRP7 MRPL1 MRPL11 MRPL13 MRPL15 MRPL16 MRPL17 MRPL19 MRPL20 MRPL22 MRPL23 MRPL25 MRPL27 MRPL28 MRPL3 MRPL31 MRPL32 MRPL33 MRPL35 MRPL36 MRPL37 MRPL39 MRPL44 MRPL49 MRPL50 MRPL51 MRPL6 MRPL8 MRPL9 MRPS12 MRPS16 MRPS17 MRPS18 MRPS28 MRPS35 MRPS5 MRPS8 MRPS9 NAM9 PET123 PPE1 RSM10 RSM18 RSM19 RSM22 RSM23 RSM24 RSM25 RSM26 RSM27 RSM28 RSM7 SWS2 VAR1 YDR115W YML6 YMR31

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	CEP3	sample cbf3 from feb 2005	Sandall S, et all (2006)
View Run	ZDS1	Sample 3 - cpn + atp, from june 2005	McCusker D, et al (2007)
View Run	MLP2	#10 Mitotic Prep1-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	NOP6	NAM9	1	Unpublished Fields Lab Data
View Screen	CAB3	NAM9	1	Hazbun TR, et al. (2003)
View Screen	CAB3	NAM9	1	Unpublished Fields Lab Data
View Screen	RHO4	NAM9	1	Unpublished Fields Lab Data
View Screen	RRB1	NAM9	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	NAM9	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..101] [213..259]	PSI-BLAST	147.450636	30S subunit
2	View Details	[102..212]	PSI-BLAST	147.450636	30S subunit
3	View Details	[260..486]	deduced	N/A	No confident structure predictions are available.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2