Protein Overview: CDC4

General Information:
Name(s) found:	CDC4 / YFL009W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|836745\|dbj\|BAA09229.1\| cell division control protein 4 [Saccharomyces cerevisiae] [NCBI NR] gi\|14318513\|ref\|NP_116585.1\| F-box protein required for G1/S and G2/M transition, associates with Skp1p and Cdc53p to form a complex, SCFCdc4, which acts as ubiquitin-protein ligase directing ubiquitination of the phosphorylated CDK inhibitor Sic1p; Cdc4p [NCBI NR] gi\|1168815\|sp\|P07834.2\|CDC4_YEAST RecName: Full=Cell division control protein 4; AltName: Full=F-box protein CDC4; AltName: Full=E3 ubiquitin ligase complex SCF subunit CDC4 [NCBI NR] pir\|\|S56245 cell division control protein CDC4 - yeast (Saccharomyces cerevisiae) [NCBI NR] Cell division control protein 4 OS=Saccharomyces cerevisiae GN=CDC4 PE=1 SV=2 [Swiss-Prot] CDC4 Cell division control protein [mips] F-box protein required for G1/S and G2/M transition, associates with Skp1p and Cdc53p to form a complex, SCFCdc4, which acts as ubiquitin-protein ligase directing ubiquitination of the phosphorylated CDK inhibitor Sic1p [SGD] CDC4, Chr VI from 116139-118478 [yeast_orfs2] Chr VI from 116139-118478 [yeastorf3.fasta] (P07834) Cell division control protein 4 [4932.FASTACCERV.txt] CDC4 SGDID:S0001885, Chr VI from 116139-118478, Verified ORF [yeastflysequest.fasta] Cdc4p [Saccharomyces cerevisiae]gi\|1084527\|pir\|\|S56245 cell division control protein CDC4 - yeast (Saccharomyces cerevisiae)gi\|836745\|dbj\|BAA09229.1\| cell division control protein 4 [Saccharomyces cerevisiae]gi\|1168815\|sp\|P07834\|CDC4_YEAST Cell divisio [nrdb_11152004_Chlamydomonas] CDC4 SGDID:S000001885, Chr VI from 116139-118478, Verified ORF, "F-box protein required for G1/S and G2/M transition, associates with Skp1p and Cdc53p to form a complex, SCFCdc4, which acts as ubiquitin-protein ligase directing ubiquitination of the phosp [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] F-box protein required for G1/S and G2/M transition, associates with Skp1p and Cdc53p to form a complex, SCFCdc4, which acts as ubiquitin-protein ligase directing ubiquitination of the phosphorylated CDK inhibitor Sic1p; Cdc4p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Cell division control protein 4 (F-box protein CDC4) (E3 ubiquitin ligase complex SCF subunit CDC4) [nr-271106-contam.fasta] cell division control protein 4 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] CDC4 SGDID:S000001885, Chr VI from 116139-118478, Verified ORF, "F-box protein required for G1/S and G2/M transition, associates with Skp1p and Cdc53p to form a complex, SCFCdc4, which acts as ubiquitin-protein ligase directing ubiquitination of the phosphorylated CDK inhibitor Sic1p" [yeast-bovrabprots.fasta] [chemostatdb.fasta] Cell division control protein 4 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=CDC4 PE=1 SV=2 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] CDC4 SGDID:S000001885, Chr VI from 116145-118484, Genome Release 64-1-1, Verified ORF, "F-box protein required for G1/S and G2/M transition, associates with Skp1p and Cdc53p to form a complex, SCFCdc4, which acts as ubiquitin-protein ligase directing ubiquitination of the phosphorylated CDK inhibitor Sic1p" [yeast-030211-contam.fasta] CDC4 SGDID:S000001885, Chr VI from 116145-118484, Genome Release 64-2-1, Verified ORF, "F-box protein required for both the G1/S and G2/M phase transitions; modular substrate specificity factor which associates with core SCF (Cdc53p, Skp1p and Hrt1p/Rbx1p) to form the SCFCdc4 complex; SCFCdc4 acts as a ubiquitin-protein ligase directing ubiquitination of cyclin-dependent kinase (CDK) phosphorylated substrates, such as: Sic1p, Far1p, Cdc6p, Clb6p, and Cln3p" [20141208_orf_trans.fasta] YFL009W SGDID:S000001885, Chr VI from 116145-118484, Genome Release 64-1-1, Verified ORF, "F-box protein required for G1/S and G2/M transition, associates with Skp1p and Cdc53p to form a complex, SCFCdc4, which acts as ubiquitin-protein ligase directing ubiquitination of the phosphorylated CDK inhibitor Sic1p" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	779 amino acids
Gene Ontology:
Cellular Component:	nucleus [IDA] nuclear matrix [IDA] SCF ubiquitin ligase complex [IDA ][IPI ] nuclear SCF ubiquitin ligase complex [IDA]
Biological Process:	G2/M transition of mitotic cell cycle [IGI ][IMP] G1/S transition of mitotic cell cycle [IMP] SCF-dependent proteasomal ubiquitin-dependent protein catabolic process [IDA ] meiosis [IMP] protein ubiquitination during ubiquitin-dependent protein catabolic process [IDA ]
Molecular Function:	ubiquitin-protein ligase activity [IDA ]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MGSFPLAEFP LRDIPVPYSY RVSGGIASSG SVTALVTAAG THRNSSTAKT VETEDGEEDI  60
   61 DEYQRKRAAG SGESTPERSD FKRVKHDNHK TLHPVNLQNT GAASVDNDGL HNLTDISNDA 120
  121 EKLLMSVDDG SAAPSTLSVN MGVASHNVAA PTTVNAATIT GSDVSNNVNS ATINNPMEEG 180
  181 ALPLSPTASS PGTTTPLAKT TKTINNNNNI ADLIESKDSI ISPEYLSDEI FSAINNNLPH 240
  241 AYFKNLLFRL VANMDRSELS DLGTLIKDNL KRDLITSLPF EISLKIFNYL QFEDIINSLG 300
  301 VSQNWNKIIR KSTSLWKKLL ISENFVSPKG FNSLNLKLSQ KYPKLSQQDR LRLSFLENIF 360
  361 ILKNWYNPKF VPQRTTLRGH MTSVITCLQF EDNYVITGAD DKMIRVYDSI NKKFLLQLSG 420
  421 HDGGVWALKY AHGGILVSGS TDRTVRVWDI KKGCCTHVFK GHNSTVRCLD IVEYKNIKYI 480
  481 VTGSRDNTLH VWKLPKESSV PDHGEEHDYP LVFHTPEENP YFVGVLRGHM ASVRTVSGHG 540
  541 NIVVSGSYDN TLIVWDVAQM KCLYILSGHT DRIYSTIYDH ERKRCISASM DTTIRIWDLE 600
  601 NIWNNGECSY ATNSASPCAK ILGAMYTLQG HTALVGLLRL SDKFLVSAAA DGSIRGWDAN 660
  661 DYSRKFSYHH TNLSAITTFY VSDNILVSGS ENQFNIYNLR SGKLVHANIL KDADQIWSVN 720
  721 FKGKTLVAAV EKDGQSFLEI LDFSKASKIN YVSNPVNSSS SSLESISTSL GLTRTTIIP

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	(MIPS) Mewes HW, et al. (2004)		CDC34 CDC4 CDC53 SGT1 SKP1
View Details	Ho Y, et al. (2002)		BOP2 CDC4 CDC53 PRB1 SGT1 SKP1 UFO1 YDR131C
View Details	Qiu et al. (2008)		CDC34 CDC4 CDC53 GRR1 HRT1 MET30 SKP1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	CEP3	sample cbf3 from feb 2005	Sandall S, et all (2006)
View Run	MLP2	#31 Asynchronous Prep (Protease cleavage)	Keck JM, et al. (2011)
View Run	MLP2	#12 Mitotic Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	SGT1	CDC4	2	Unpublished Fields Lab Data
View Screen	SKP1	CDC4	2	Uetz P, et al. (2000)
View Screen	SGT1	CDC4	2	Unpublished Fields Lab Data
View Screen	SKP1	CDC4	2	Unpublished Fields Lab Data
View Screen	SGT1	CDC4	2	Unpublished Fields Lab Data
View Screen	SKP1	CDC4	2	Unpublished Fields Lab Data
View Screen	SGT1	CDC4	2	Unpublished Fields Lab Data
View Screen	CDC4	YPR092W	2	Unpublished Fields Lab Data
View Screen	SGT1	CDC4	2	Unpublished Fields Lab Data
View Screen	SGT1	CDC4	2	Unpublished Fields Lab Data
View Screen	CWC2	CDC4	1	Hazbun TR, et al. (2003)
View Screen	CDC4	STE14	1	Unpublished Fields Lab Data
View Screen	CDC4	ECM29	1	Unpublished Fields Lab Data
View Screen	CDC4	YKR023W	1	Unpublished Fields Lab Data
View Screen	CDC4	VPS68	1	Unpublished Fields Lab Data
View Screen	CDC4	YDR431W	1	Unpublished Fields Lab Data
View Screen	CDC4	HXT1	1	Unpublished Fields Lab Data
View Screen	CDC4	YKR043C	1	Unpublished Fields Lab Data
View Screen	CDC4	RRP40	1	Unpublished Fields Lab Data
View Screen	CDC4	YDR445C	1	Unpublished Fields Lab Data
View Screen	CDC4	RIX1	1	Unpublished Fields Lab Data
View Screen	CDC4	TVP38	1	Unpublished Fields Lab Data
View Screen	CDC4	VPS21	1	Unpublished Fields Lab Data
View Screen	CDC4	MFA1	1	Unpublished Fields Lab Data
View Screen	CDC4	YHR219W	1	Unpublished Fields Lab Data
View Screen	CDC4	AQY2	1	Unpublished Fields Lab Data
View Screen	CDC4	YRR1	1	Unpublished Fields Lab Data
View Screen	CDC4	MDM10	1	Unpublished Fields Lab Data
View Screen	CDC4	RIB3	1	Unpublished Fields Lab Data
View Screen	CDC4	TMA108	1	Unpublished Fields Lab Data
View Screen	CDC4	PSR2	1	Unpublished Fields Lab Data
View Screen	CDC4	PEX27	1	Unpublished Fields Lab Data
View Screen	CDC4	KTR4	1	Unpublished Fields Lab Data
View Screen	CDC4	SBH2	1	Unpublished Fields Lab Data
View Screen	CDC4	YIL172C, FSP2	1	Unpublished Fields Lab Data
View Screen	CDC4	RIC1	1	Unpublished Fields Lab Data
View Screen	CDC4	ISU2	1	Unpublished Fields Lab Data
View Screen	CDC4	TSC10	1	Unpublished Fields Lab Data
View Screen	CDC4	ERG28	1	Unpublished Fields Lab Data
View Screen	CDC4	DAL3	1	Unpublished Fields Lab Data
View Screen	CDC4	BOS1	1	Unpublished Fields Lab Data
View Screen	CDC4	YOR282W	1	Unpublished Fields Lab Data
View Screen	CDC4	YCR013C	1	Unpublished Fields Lab Data
View Screen	CDC4	SER3	1	Unpublished Fields Lab Data
View Screen	CDC4	YJL022W	1	Unpublished Fields Lab Data
View Screen	CDC4	GIS4	1	Unpublished Fields Lab Data
View Screen	CDC4	YOR300W	1	Unpublished Fields Lab Data
View Screen	CDC4	AAD3	1	Unpublished Fields Lab Data
View Screen	CDC4	RAD51	1	Unpublished Fields Lab Data
View Screen	CDC4	MAD2	1	Unpublished Fields Lab Data
View Screen	CDC4	ABF2	1	Unpublished Fields Lab Data
View Screen	CDC4	VTS1	1	Unpublished Fields Lab Data
View Screen	CDC4	YDL025C	1	Unpublished Fields Lab Data
View Screen	CDC4	IRC7	1	Unpublished Fields Lab Data
View Screen	CDC4	GYP6	1	Unpublished Fields Lab Data
View Screen	CDC4	DYN3	1	Unpublished Fields Lab Data
View Screen	CDC4	PDR12	1	Unpublished Fields Lab Data
View Screen	CDC4	BUG1	1	Unpublished Fields Lab Data
View Screen	CDC4	MNP1	1	Unpublished Fields Lab Data
View Screen	CDC4	SET4	1	Unpublished Fields Lab Data
View Screen	CDC4	FKS3	1	Unpublished Fields Lab Data
View Screen	CDC4	MSD1	1	Unpublished Fields Lab Data
View Screen	CDC4	YDL157C	1	Unpublished Fields Lab Data
View Screen	CDC4	RPB9	1	Unpublished Fields Lab Data
View Screen	CDC4	RCY1	1	Unpublished Fields Lab Data
View Screen	CDC4	RRT16	1	Unpublished Fields Lab Data
View Screen	CDC4	POS5	1	Unpublished Fields Lab Data
View Screen	CDC4	AAD4	1	Unpublished Fields Lab Data
View Screen	CDC4	CDC43	1	Unpublished Fields Lab Data
View Screen	CDC4	ILM1	1	Unpublished Fields Lab Data
View Screen	CDC4	RPC31	1	Unpublished Fields Lab Data
View Screen	CDC4	GCV1	1	Unpublished Fields Lab Data
View Screen	CDC4	YGR018C	1	Unpublished Fields Lab Data
View Screen	CDC4	OAR1	1	Unpublished Fields Lab Data
View Screen	CDC4	SQS1	1	Unpublished Fields Lab Data
View Screen	CDC4	YPR114W	1	Unpublished Fields Lab Data
View Screen	CDC4	RLI1	1	Unpublished Fields Lab Data
View Screen	CDC4	PPT1	1	Unpublished Fields Lab Data
View Screen	CDC4	MBR1	1	Unpublished Fields Lab Data
View Screen	CDC4	YNR021W	1	Unpublished Fields Lab Data
View Screen	CDC53	CDC4	1	Unpublished Fields Lab Data
View Screen	CDC4	MCM21	1	Unpublished Fields Lab Data
View Screen	CDC4	COS6	1	Unpublished Fields Lab Data
View Screen	CDC4	YKL171W	1	Unpublished Fields Lab Data
View Screen	CDC4	AIF1	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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No microscopy data found in the PDR for this protein.

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..107]	deduced	N/A	No confident structure predictions are available.
2	View Details	[108..450]	PSI-BLAST	27.39794	Groucho/tle1, C-terminal domain
3	View Details	[451..601]	PSI-BLAST	225.9794	Tup1, C-terminal domain
4	View Details	[602..779]	ORFEUS	14.28	Quinohemoprotein amine dehydrogenase B chain

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.68

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2