Protein Overview: BEM1

General Information:
Name(s) found:	BEM1 / YBR200W [SGD]
Description(s) found: Found 29 descriptions. SHOW ALL	Protein containing SH3-domains, involved in establishing cell polarity and morphogenesis; functions as a scaffold protein for complexes that include Cdc24p, Ste5p, Ste20p, and Rsr1p [SGD]
Organism:	Saccharomyces cerevisiae
Length:	551 amino acids
Gene Ontology:
Cellular Component:	incipient cellular bud site [IDA] cellular bud tip [IDA] cellular bud neck [IDA] mating projection tip [TAS]
Biological Process:	cell morphogenesis involved in conjugation with cellular fusion [IGI][IMP][TAS]
Molecular Function:	protein binding [IDA][IMP] phosphatidylinositol-3-phosphate binding [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MLKNFKLSKR DSNGSKGRIT SADISTPSHD NGSVIKHIKT VPVRYLSSSS TPVKSQRDSS  60
   61 PKNRHNSKDI TSPEKVIKAK YSYQAQTSKE LSFMEGEFFY VSGDEKDWYK ASNPSTGKEG 120
  121 VVPKTYFEVF DRTKPSSVNG SNSSSRKVTN DSLNMGSLYA IVLYDFKAEK ADELTTYVGE 180
  181 NLFICAHHNC EWFIAKPIGR LGGPGLVPVG FVSIIDIATG YATGNDVIED IKSVNLPTVQ 240
  241 EWKSNIARYK ASNISLGSVE QQQQQSITKP QNKSAKLVDG ELLVKASVES FGLEDEKYWF 300
  301 LVCCELSNGK TRQLKRYYQD FYDLQVQLLD AFPAEAGKLR DAGGQWSKRI MPYIPGPVPY 360
  361 VTNSITKKRK EDLNIYVADL VNLPDYISRS EMVHSLFVVL NNGFDREFER DENQNNIKTL 420
  421 QENDTATFAT ASQTSNFAST NQDNTLTGED LKLNKKLSDL SLSGSKQAPA QSTSGLKTTK 480
  481 IKFYYKDDIF ALMLKGDTTY KELRSKIAPR IDTDNFKLQT KLFDGSGEEI KTDSQVSNII 540
  541 QAKLKISVHD I

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	(MIPS) Mewes HW, et al. (2004)		ABP1 AIP1 ARC40 ARP2 BEM1 BUD6 CAP1 CAP2 COF1 CRN1 DLD2 GLK1 LAS17 OYE2 PAN1 PFY1 RVS167 SAC6 SLA1 SLA2 SRV2 TPM1 TPM2 TWF1 VRP1
View Details	Gavin AC, et al. (2002)		BEM1 BOI2 CDC24 RSC2
View Details	Qiu et al. (2008)		ABP1 ACT1 BEM1 BNI1 BNR1 BOI1 BOI2 BUD6 CDC11 CDC12 CDC24 CDC3 CDC42 COF1 GYL1 HBT1 HOF1 LAS17 LSB3 MLC1 MSB1 MYO1 MYO2 OSH2 PEA2 PFY1 RHO1 ROM2 RVS161 SLA1 SLA2 SPA2 STE20 STE4 TPM1 ZDS1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	PAN1	No Comments	Toshima J, et al (2006)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	CDC24	BEM1	2	Drees BL, et al. (2001)
View Screen	BEM1	CDC24	2	Drees BL, et al. (2001)
View Screen	BEM1	YEL043W	2	Unpublished Fields Lab Data
View Screen	BEM1	SEC15	2	Drees BL, et al. (2001)
View Screen	BOI2	BEM1	2	Drees BL, et al. (2001)
View Screen	BEM1	CAF130	2	Unpublished Fields Lab Data
View Screen	CDC42	BEM1	2	Drees BL, et al. (2001)
View Screen	BEM1	FAR1	2	Drees BL, et al. (2001)
View Screen	CDC42	BEM1	2	Unpublished Fields Lab Data
View Screen	BEM1	SWE1	2	Drees BL, et al. (2001)
View Screen	BEM1	YIR014W	2	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	BEM1	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..66]	deduced	N/A	No confident structure predictions are available.
2	View Details	[67..133]	PSI-BLAST	91.24799	c-src protein tyrosine kinase; c-src tyrosine kinase
3	View Details	[134..238]	PSI-BLAST	91.24799	c-src protein tyrosine kinase; c-src tyrosine kinase
4	View Details	[239..366]	PSI-BLAST	91.24799	c-src protein tyrosine kinase; c-src tyrosine kinase
5	View Details	[367..434]	PSI-BLAST	77.156545	p47phox NADPH oxidase
6	View Details	[435..551]	PSI-BLAST	405.144602	Bud emergence mediator Bemp1

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
binding	3.12724619324809	bayes_pls_golite062009
signal transducer activity	2.5284324342567	bayes_pls_golite062009
molecular transducer activity	2.5284324342567	bayes_pls_golite062009
protein binding	2.14586566310919	bayes_pls_golite062009
molecular adaptor activity	1.33740973673697	bayes_pls_golite062009
SH3/SH2 adaptor activity	1.2323381312171	bayes_pls_golite062009
protein binding, bridging	0.83823250804321	bayes_pls_golite062009
cytoskeletal protein binding	0.83493091348808	bayes_pls_golite062009
actin filament binding	0.75424908168927	bayes_pls_golite062009
transcription regulator activity	0.540818657754841	bayes_pls_golite062009
enzyme regulator activity	0.456661966897198	bayes_pls_golite062009
nucleic acid binding	0.352421112301971	bayes_pls_golite062009
DNA binding	0.281643350069021	bayes_pls_golite062009
phosphoprotein binding	0.16798254471727	bayes_pls_golite062009

No functions predicted.

Term	Confidence	Notes
binding	2.24212855645397	bayes_pls_golite062009
protein binding	1.04550817342273	bayes_pls_golite062009
signal transducer activity	0.639296022362811	bayes_pls_golite062009
molecular transducer activity	0.639296022362811	bayes_pls_golite062009
GTPase regulator activity	0.459633124432679	bayes_pls_golite062009
nucleoside-triphosphatase regulator activity	0.457933659119243	bayes_pls_golite062009
small GTPase regulator activity	0.390993828682912	bayes_pls_golite062009
enzyme regulator activity	0.217422685675578	bayes_pls_golite062009
phosphotransferase activity, alcohol group as acceptor	0.187012142094005	bayes_pls_golite062009
protein serine/threonine kinase activity	0.0693380673720717	bayes_pls_golite062009
protein kinase activity	0.0278354564906339	bayes_pls_golite062009
kinase activity	0.00999242592237526	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.98

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2