Protein Overview: YBR090C-A, NHP6B

General Information:
Name(s) found:	YBR090C-A [SGD] NHP6B / YBR089C-A [SGD]
Description(s) found: SHOW ONLY BEST	pir\|\|S78076 nonhistone chromosomal protein NHP6B - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|6319565\|ref\|NP_009647.1\| High-mobility group non-histone chromatin protein, functionally redundant with Nhp6Ap; homologous to mammalian high mobility group proteins 1 and 2; acts to recruit transcription factor Rcs1p to certain promoters; Nhp6bp [Sacch [NCBI NR] gi\|38503394\|sp\|P11633.2\|NHP6B_YEAST RecName: Full=Non-histone chromosomal protein 6B [NCBI NR] gi\|1870104\|emb\|CAA85042.1\| NHP6B [Saccharomyces cerevisiae] [NCBI NR] (P11633) Nonhistone chromosomal protein 6B [Swiss-Prot] Non-histone chromosomal protein 6B OS=Saccharomyces cerevisiae GN=NHP6B PE=1 SV=2 [Swiss-Prot] NHP6B Nonhistone chromosomal protein [mips] Chromosome II from 426443 to 426144 [SGD] High-mobility group (HMG) protein that binds to and remodels nucleosomes; involved in recruiting FACT and other chromatin remodelling complexes to the chromosomes; functionally redundant with Nhp6Ap; homologous to mammalian HMGB1 and HMGB2 [SGD] NHP6B, Chr II from 426148-426447, reverse complement [yeast_orfs2] Chr II from 426148-426447, reverse complement [yeastorf3.fasta] Chr II from 426445-426146, reverse complement [yeastorf5.fasta] NHP6B SGDID:S0002157, Chr II from 426446-426147, reverse complement, Verified ORF [yeastflysequest.fasta] NHP6B SGDID:S0002157, Chr II from 426483-426184, reverse complement, Verified ORF [10112005cervSGD.fasta] Homologous to mammalian high mobility group proteins 1 and 2; functions redundantly with the highly homologous gene, NHP6A; high-mobility group non-histone chromatin protein; 11-kDa nonhistone chromosomal protein [Saccharomyces cerevisiae]gi\|1870104\|emb\| [nrdb_11152004_Chlamydomonas] NHP6B SGDID:S000002157, Chr II from 426483-426184, reverse complement, Verified ORF, "High-mobility group non-histone chromatin protein, functionally redundant with Nhp6Ap; homologous to mammalian high mobility group proteins 1 and 2; acts to recruit tran [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] Nonhistone chromosomal protein 6B [nr-271106-contam.fasta] High-mobility group non-histone chromatin protein, functionally redundant with Nhp6Ap; homologous to mammalian high mobility group proteins 1 and 2; acts to recruit transcription factor Rcs1p to certain promoters; Nhp6bp [Saccharomyces cerevisiae] [nr-271106-contam.fasta] NHP6B [Saccharomyces cerevisiae] [nr-271106-contam.fasta] NHP6B SGDID:S000002157, Chr II from 426483-426184, reverse complement, Verified ORF, "High-mobility group non-histone chromatin protein, functionally redundant with Nhp6Ap; homologous to mammalian high mobility group proteins 1 and 2; acts to recruit transcription factor Rcs1p to certain promoters" [yeast-bovrabprots.fasta] [chemostatdb.fasta]
Organism:	Saccharomyces cerevisiae
Length:	99 amino acids
Gene Ontology:
Cellular Component:	nuclear chromatin [ISS]
Biological Process:	chromatin remodeling [IMP] chromatin organization [ISS] positive regulation of transcription from RNA polymerase II promoter [IMP][IPI] regulation of transcription from RNA polymerase III promoter [IDA][IGI][IMP] regulation of transcription from RNA polymerase II promoter [ISS]
Molecular Function:	chromatin binding [IDA][ISS]

Sequence:
Sequence: [PDR BLAST] [ProtParam]	1 11 21 31 41 51 \| \| \| \| \| \| 1 MAATKEAKQP KEPKKRTTRR KKDPNAPKRR LSAYMFFANE NRDIVRSENP DVTFGQVGRI 60 61 LGERWKALTA EEKQPYESKA QADKKRYESE KELYNATRA

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Gavin AC, et al. (2002)		ADE5,7 HHF1, HHF2 IOC3 ISW1 ISW2 ITC1 MOT1 NPL6 REB1 RFA1 RFX1 RNR2 RPC34 RPC40 RPO31 RSC2 RSC3 RSC58 RSC8 RVB2 SFH1 STH1 STO1 TAF1 TFC7 TOP1 VPS1 YBR090C-A, NHP6B
View Details	Qiu et al. (2008)		NHP6A PCC1 YBR090C-A, NHP6B

Mass Spectrometry Data

No mass spectrometry results found for this protein.

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

No yeast two-hybrid interactions found for this protein.

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	YBR090C-A, NHP6B	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..99]	PSI-BLAST	284.0103	NHP6a

Functions predicted (by domain):

#

Gene Ontology predictions

1

Term	Confidence	Notes
DNA binding	5.15324651287773	bayes_pls_golite062009
nucleic acid binding	5.01228762819617	bayes_pls_golite062009
transcription regulator activity	4.9607192917135	bayes_pls_golite062009
sequence-specific DNA binding	4.06355503548774	bayes_pls_golite062009
transcription factor activity	3.82658263870186	bayes_pls_golite062009
binding	3.6211482127468	bayes_pls_golite062009
transcription activator activity	2.95665802871704	bayes_pls_golite062009
RNA polymerase II transcription factor activity	2.75319687398598	bayes_pls_golite062009
protein binding	2.18211240370246	bayes_pls_golite062009
chromatin binding	1.64428658020027	bayes_pls_golite062009
transcription repressor activity	1.53825678633232	bayes_pls_golite062009
transcription factor binding	1.10315330167937	bayes_pls_golite062009
DNA bending activity	0.87985209274756	bayes_pls_golite062009
crossed form four-way junction DNA binding	0.293183929205961	bayes_pls_golite062009
bent DNA binding	0.293183929205961	bayes_pls_golite062009
open form four-way junction DNA binding	0.293183929205961	bayes_pls_golite062009
RNA polymerase II transcription factor activity, enhancer binding	0.261169200304829	bayes_pls_golite062009

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

YRC Informatics Platform - Version 3.0
Created and Maintained by: Michael Riffle