Protein Overview: RPC25

General Information:
Name(s) found:	RPC25 / YKL144C [SGD]
Description(s) found: Found 35 descriptions. SHOW ALL	RNA polymerase III subunit C25, required for transcription initiation; forms a heterodimer with Rpc17p; paralog of Rpb7p [SGD]
Organism:	Saccharomyces cerevisiae
Length:	212 amino acids
Gene Ontology:
Cellular Component:	DNA-directed RNA polymerase III complex [TAS]
Biological Process:	transcription from RNA polymerase III promoter [TAS]
Molecular Function:	DNA-directed RNA polymerase activity [TAS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MFILSKIADL VRIPPDQFHR DTISAITHQL NNKFANKIIP NVGLCITIYD LLTVEEGQLK  60
   61 PGDGSSYINV TFRAVVFKPF LGEIVTGWIS KCTAEGIKVS LLGIFDDIFI PQNMLFEGCY 120
  121 YTPEESAWIW PMDEETKLYF DVNEKIRFRI EREVFVDVKP KSPKERELEE RAQLENEIEG 180
  181 KNEETPQNEK PPAYALLGSC QTDGMGLVSW WE

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		RET1 RPB5 RPB8 RPC11 RPC17 RPC19 RPC25 RPC31 RPC34 RPC37 RPC40 RPC53 RPC82 RPO26 RPO31
View Details	(MIPS) Mewes HW, et al. (2004)		RET1 RPB10 RPB5 RPB8 RPC10 RPC19 RPC25 RPC31 RPC34 RPC40 RPC82 RPO26 RPO31
View Details	Krogan NJ, et al. (2006)		BDP1 CDC14 COY1 FAR7 MAF1 RET1 RPA12 RPA135 RPA14 RPA190 RPA34 RPA43 RPA49 RPC10 RPC11 RPC17 RPC19 RPC25 RPC31 RPC34 RPC37 RPC40 RPC53 RPC82 RPO31 YDL144C YIR024C
View Details	Gavin AC, et al. (2006)		RET1 RPC25 RPC31 RPC34 RPC37 RPC40 RPC53 RPC82 RPO31
View Details	Gavin AC, et al. (2002)		AMS1 ASC1 CDC14 DHH1 FBA1 MAF1 RET1 RPA12 RPA135 RPA190 RPA43 RPA49 RPB10 RPB5 RPB8 RPC11 RPC17 RPC19 RPC25 RPC31 RPC34 RPC37 RPC40 RPC53 RPC82 RPO26 RPO31 RSC8 SRO7
View Details	Ho Y, et al. (2002)		ACC1 ACH1 ADH2 ADR1 GCD11 GFA1 GND1 GUS1 IDH2 ISA2 MDH1 MET18 NGL2 PYC1 PYC2 RET1 RPA135 RPA190 RPA49 RPB5 RPC19 RPC25 RPC34 RPC40 RPC82 RPN3 RPO26 RPO31 RVS167 SEC27 TBS1 TFP1 TSA2 YFL042C YHR112C
View Details	Qiu et al. (2008)		RET1 RPA12 RPA135 RPA14 RPA190 RPA34 RPA43 RPB10 RPB11 RPB3 RPB4 RPB5 RPB8 RPC10 RPC11 RPC17 RPC19 RPC25 RPC31 RPC34 RPC37 RPC40 RPC53 RPC82 RPO26 RPO31

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	CEP3	sample cbf3 from feb 2005	Sandall S, et all (2006)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	LOS1	RPC25	2	Uetz P, et al. (2000)

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	RPC25	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..77]	PSI-BLAST	148.37734	C-terminal domain of RNA polymerase II subunit RBP4 (RpoE); N-terminal, heterodimerisation domain of RBP4 (RpoE)
2	View Details	[78..212]	PSI-BLAST	148.37734	C-terminal domain of RNA polymerase II subunit RBP4 (RpoE); N-terminal, heterodimerisation domain of RBP4 (RpoE)

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.98

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2