Protein Overview: RTT101

General Information:
Name(s) found:	RTT101 / YJL047C [SGD]
Description(s) found: Found 35 descriptions. SHOW ALL	Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex with a role in anaphase progression; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by the ubiquitin-like protein, Rub1p [SGD]
Organism:	Saccharomyces cerevisiae
Length:	842 amino acids
Gene Ontology:
Cellular Component:	nucleus [IDA] cytoplasm [IDA]
Biological Process:	regulation of mitosis [IMP] negative regulation of transposition, DNA-mediated [IMP] ubiquitin-dependent protein catabolic process [IC]
Molecular Function:	ubiquitin-protein ligase activity [IDA] protein binding [IPI ]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MINESVSKRE GFHESISRET SASNALGLYN KFNDERNPRY RTMIAELHEF FHLTLAETIT  60
   61 ETDVKELECN KEKAAKFRKL MPKMLNNCRE LTQRKSYIPY NSEFNGNDEK QKKFQLLHQH 120
  121 QIVLSFQEFC DELAKLIIDA HVLSFLTRCD YSYEIIPKNW TSFYKLFQYV MGAVGPIISY 180
  181 VPVNYPMIRK ELGFETLTIF QYYDSKLFEC MKSHFGREFS TLVSATIHHY IHMFPITNTM 240
  241 LEKEVPMLRI MSNCNFSIEG LSPKDFYMKT LRQYYCEESN LRPRLETFKN FKVLLTRNAL 300
  301 LASLFSPEWV SDANDLFISH LLLNKKSISE YIEIGKDTYD EEKERYFKTE THFSLLMFRN 360
  361 AFEAKNMLSK FKEFCDDAVS EKLKAAYGSN HDTERLFDEV VQLANVDHLK IYSDSIEYHL 420
  421 CNLLGSTSKA IEQYVKYFES HLFIIVRKIK TTKKDLPRDM KIKYLNENLP ILRLKFVNLP 480
  481 TFPNFFERSI FRKTILQSDQ NSSFIKDILP VYKDSLMELF KQRIITNVSQ EDEMRYRDQY 540
  541 QPYLSQFFQP VEVMADLRIK YASFLSFYEN IEAAVKFGKT YNENNSKSFF PLIFDRERIP 600
  601 KVFQQSNEVK KNFVLPQEMD DTWNQFLRNY HEQNKVEDSD ASKKELYPMW NLHHCEVESP 660
  661 YIIQDGTNLI FELTLFQTCV LTLFNESDHL TLQVISEQTK LAYKDLALVL KSFCNYKILT 720
  721 RDIDNTYSIN ESFKPDMKKV KNGKLRVVLP RTASLQSSNT GGERTSSAHH EGSNSQWTQE 780
  781 LLKACITRSV KSERNGLDYD HLFETVKQQI KGFSVGEFKD ALAKLLRDKF ITRDESTATY 840
  841 KY

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		CDC34 CDC53 RTG2 RTT101
View Details	Krogan NJ, et al. (2006)		CAK1 CDC34 CDC53 CDC9 CRT10 GRR1 MEP3 MET30 MMS1 POR1 RTT101 SAF1
View Details	Ho Y, et al. (2002)		CDC53 GDH2 GUF1 HRT1 IDH1 MMS22 RIX7 RPN8 RTT101 RTT107 SEC27

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	MLP2	#32 Asynchronous Prep-No Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#31 Asynchronous Prep (Protease cleavage)	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	RTT101	OPI3	2	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	RTT101	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..369] [383..396]	PSI-BLAST	164.0	Anaphase promoting complex (APC); Cullin homolog 1, Cul-1; Cullin homolog 1, cul-1
2	View Details	[370..382] [397..551]	PSI-BLAST	164.0	Anaphase promoting complex (APC); Cullin homolog 1, Cul-1; Cullin homolog 1, cul-1
3	View Details	[552..649]	PSI-BLAST	164.0	Anaphase promoting complex (APC); Cullin homolog 1, Cul-1; Cullin homolog 1, cul-1
4	View Details	[650..773]	PSI-BLAST	164.0	Anaphase promoting complex (APC); Cullin homolog 1, Cul-1; Cullin homolog 1, cul-1
5	View Details	[774..842]	PSI-BLAST	164.0	Anaphase promoting complex (APC); Cullin homolog 1, Cul-1; Cullin homolog 1, cul-1

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
transcription regulator activity	4.44907471960058	bayes_pls_golite062009
transcription factor activity	3.48782936574155	bayes_pls_golite062009
ubiquitin-protein ligase activity	3.04993477724372	bayes_pls_golite062009
small conjugating protein ligase activity	3.01842842236076	bayes_pls_golite062009
transcription repressor activity	2.39237599101586	bayes_pls_golite062009
binding	2.3219000606044	bayes_pls_golite062009
acid-amino acid ligase activity	2.07503944671713	bayes_pls_golite062009
transcription activator activity	1.69071209162619	bayes_pls_golite062009
ligase activity	1.56500586430034	bayes_pls_golite062009
RNA polymerase II transcription factor activity	1.2152419300113	bayes_pls_golite062009
nucleic acid binding	1.19889127256113	bayes_pls_golite062009
protein binding	1.15192193279649	bayes_pls_golite062009
DNA binding	0.906045577633981	bayes_pls_golite062009
structure-specific DNA binding	0.851238175736283	bayes_pls_golite062009
ligase activity, forming carbon-nitrogen bonds	0.602749768906734	bayes_pls_golite062009
catalytic activity	0.201606344181603	bayes_pls_golite062009
transcription factor binding	0.18566280876224	bayes_pls_golite062009
bubble DNA binding	0.163558276321259	bayes_pls_golite062009
specific transcriptional repressor activity	0.0565989703351002	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.91

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2