Protein Overview: RPS12

General Information:
Name(s) found:	RPS12 / YOR369C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6324945\|ref\|NP_015014.1\| Protein component of the small (40S) ribosomal subunit; has similarity to rat ribosomal protein S12; Rps12p [Saccharomyces cerevisiae] [NCBI NR] pir\|\|S62102 ribosomal protein S12.e, cytosolic - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|1420798\|emb\|CAA99700.1\| RS12 [Saccharomyces cerevisiae] [NCBI NR] gi\|1350929\|sp\|P48589.1\|RS12_YEAST RecName: Full=40S ribosomal protein S12 [NCBI NR] gi\|1051279\|gb\|AAA80546.1\| ribosomal protein S12 [NCBI NR] 40S ribosomal protein S12 OS=Saccharomyces cerevisiae GN=RPS12 PE=1 SV=1 [Swiss-Prot] RPS12 40S small subunit acidic ribosomal protein S12 [mips] Protein component of the small (40S) ribosomal subunit; has similarity to rat ribosomal protein S12 [SGD] (P48589) 40S ribosomal protein S12 [4932.FASTACCERV.txt] Chr XV from 1028619-1028188, reverse complement [hmr_yeast5_DM3-1_pombe.fasta] Chr XV from 1028187-1028618, reverse complement [yeastorf3.fasta] Chr XV from 1028188-1028619, reverse complement [yeastorf4.fasta] RPS12, Chr XV from 1028187-1028618, reverse complement [yeast_orfs2] RPS12 SGDID:S0005896, Chr XV from 1028621-1028190, reverse complement, Verified ORF [yeastflysequest.fasta] Protein component of the small (40S) ribosomal subunit; has similarity to rat ribosomal protein S12 [Saccharomyces cerevisiae]gi\|1420798\|emb\|CAA99700.1\| RS12 [Saccharomyces cerevisiae]gi\|2119085\|pir\|\|S62102 ribosomal protein S12.e, cytosolic - yeast (Sa [nrdb_11152004_Chlamydomonas] RPS12 SGDID:S000005896, Chr XV from 1028621-1028190, reverse complement, Verified ORF, "Protein component of the small (40S) ribosomal subunit; has similarity to rat ribosomal protein S12" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] RPS12 SGDID:S000005896, Chr XV from 1028623-1028192, reverse complement, Verified ORF, "Protein component of the small (40S) ribosomal subunit; has similarity to rat ribosomal protein S12" [yeast-contam.fasta] RS12 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] ribosomal protein S12 [nr-271106-contam.fasta] Protein component of the small (40S) ribosomal subunit; has similarity to rat ribosomal protein S12; Rps12p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] 40S ribosomal protein S12 [nr-271106-contam.fasta] [chemostatdb.fasta] 40S ribosomal protein S12 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=RPS12 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] RPS12 SGDID:S000005896, Chr XV from 1028625-1028194, Genome Release 64-1-1, reverse complement, Verified ORF, "Protein component of the small (40S) ribosomal subunit; has similarity to rat ribosomal protein S12" [yeast-030211-contam.fasta] RPS12 SGDID:S000005896, Chr XV from 1028625-1028194, Genome Release 64-2-1, reverse complement, Verified ORF, "Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S12, no bacterial homolog" [20141208_orf_trans.fasta] TR6245\|c0_g1_i1\|g.4385 ORF TR6245\|c0_g1_i1\|g.4385 TR6245\|c0_g1_i1\|m.4385 type:complete len:144 (+) TR6245\|c0_g1_i1:76-507(+) [Y12_S_cerevisiae-Sep2015-contam.fasta] TR6683\|c0_g1_i1\|g.4833 ORF TR6683\|c0_g1_i1\|g.4833 TR6683\|c0_g1_i1\|m.4833 type:complete len:144 (+) TR6683\|c0_g1_i1:126-557(+) [DBVPG6044_S_cerevisiae-Sep2015-contam.fasta] YOR369C SGDID:S000005896, Chr XV from 1028625-1028194, Genome Release 64-1-1, reverse complement, Verified ORF, "Protein component of the small (40S) ribosomal subunit; has similarity to rat ribosomal protein S12" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta] asmbl_2939\|g.1571 ORF asmbl_2939\|g.1571 asmbl_2939\|m.1571 type:complete len:144 (+) asmbl_2939:69-500(+) [DBVPG6044_Trinity_v2_2_0_PASA_6May2016-contam.fasta] asmbl_3052\|g.1775 ORF asmbl_3052\|g.1775 asmbl_3052\|m.1775 type:complete len:144 (+) asmbl_3052:69-500(+) [Y12_Trinity_v2_2_0_PASA_6May2016-contam.fasta] ID=asmbl_2944\|g.1831 ORF ID=asmbl_2944\|g.1831 ID=asmbl_2944\|m.1831 type:complete len:144 (+) ID=asmbl_2944:69-500(+) [DBVPG6044_quiverGenomes_Trinity_v2_2_0_PASA_2June2016-contam.fasta] ID=asmbl_3051\|g.1828 ORF ID=asmbl_3051\|g.1828 ID=asmbl_3051\|m.1828 type:complete len:144 (+) ID=asmbl_3051:69-500(+) [Y12_quiver_Trinity_v2_2_0_PASA_2June2016-contam.fasta]
Organism:	Saccharomyces cerevisiae
Length:	143 amino acids
Gene Ontology:
Cellular Component:	ribonucleoprotein complex [IEA] cytosolic small ribosomal subunit [IDA] cytoplasm [IEA] ribosome [IEA] intracellular [IEA]
Biological Process:	translation [TAS]
Molecular Function:	structural constituent of ribosome [TAS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSDVEEVVEV QEETVVEQTA EVTIEDALKV VLRTALVHDG LARGLRESTK ALTRGEALLV  60
   61 VLVSSVTEAN IIKLVEGLAN DPENKVPLIK VADAKQLGEW AGLGKIDREG NARKVVGASV 120
  121 VVVKNWGAET DELSMIMEHF SQQ

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		ECM10 RPL4A RPS12 RPS14A RPS16B, RPS16A
View Details	(MIPS) Mewes HW, et al. (2004)		ASC1 RPS0A RPS0B RPS10A RPS10B RPS11A, RPS11B RPS12 RPS13 RPS14A RPS14B RPS15 RPS16B, RPS16A RPS17A RPS17B RPS18B, RPS18A RPS19A RPS19B RPS1A RPS1B RPS2 RPS20 RPS21A RPS21B RPS22A RPS22B RPS23B, RPS23A RPS24A, RPS24B RPS25A RPS25B RPS26A RPS26B RPS27A RPS27B RPS28A RPS28B RPS29A RPS29B RPS3 RPS30B, RPS30A RPS31 RPS4B, RPS4A RPS5 RPS6A, RPS6B RPS7A RPS7B RPS8B, RPS8A RPS9A RPS9B
View Details	Qiu et al. (2008)		ASC1 ATG27 BUD20 EFT2, EFT1 FYV12 MRPS8 NAT3 RPL10 RPL11A RPL11B RPL12B, RPL12A RPL13A RPL13B RPL14A RPL14B RPL15A RPL15B RPL16A RPL16B RPL17A RPL17B RPL18A, RPL18B RPL19B, RPL19A RPL1A, RPL1B RPL20A RPL20B RPL21A RPL21B RPL22A RPL23B, RPL23A RPL24A RPL24B RPL25 RPL26A RPL26B RPL27A RPL27B RPL28 RPL29 RPL2A, RPL2B RPL3 RPL30 RPL31A RPL31B RPL32 RPL33A RPL33B RPL34A RPL35A, RPL35B RPL36A RPL36B RPL37A RPL37B RPL38 RPL39 RPL40B, RPL40A RPL41A, RPL41B RPL42B, RPL42A RPL43B, RPL43A RPL4A RPL5 RPL6A RPL6B RPL7A RPL8A RPL8B RPL9A RPL9B RPP0 RPP1A RPP1B RPP2A RPP2B RPS0A RPS0B RPS10A RPS10B RPS11A, RPS11B RPS12 RPS13 RPS14A RPS14B RPS15 RPS16B, RPS16A RPS17A RPS17B RPS18B, RPS18A RPS19A RPS19B RPS1A RPS1B RPS2 RPS20 RPS21A RPS21B RPS22A RPS22B RPS23B, RPS23A RPS24A, RPS24B RPS25A RPS25B RPS26A RPS26B RPS27A RPS27B RPS28A RPS28B RPS29A RPS29B RPS3 RPS30B, RPS30A RPS31 RPS4B, RPS4A RPS5 RPS6A, RPS6B RPS7A RPS7B RPS8B, RPS8A RPS9A RPS9B RRP5 TEF1, TEF2 TSR1 VAR1 VMA6 YML6

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	TOR2	No Comments	Reinke A, et al. (2004)
View Run	LST8	No Comments	Reinke A, et al. (2004)
View Run	TOR2	No Comments	Reinke A, et al. (2004)
View Run	RPA135	his-HA tag on RPA135	Schneider, DA, et al. (2006)
View Run	DAD1	Dam1-765 complex purified from yeast with Dad1-TAP	Shimogawa MM, et al. (2006)
View Run	ASF1	3908: TAP-tagged, includes hir2(delta) in background	Green EM, et al (2005)
View Run	DAD1	Dam1-765 complex purified from yeast with Dad1-TAP	Shimogawa MM, et al. (2006)
View Run	ZDS1	Sample 2- cpn2 from june 2005	McCusker D, et al (2007)
View Run	BOI1	Sample boi 1 with ha tag from october 2005	McCusker D, et al (2007)
View Run	BOI1	Sample boi1 with gst from october 2005	McCusker D, et al (2007)
View Run	BOI1	Sample bob1 (2nd set) from october 2005	McCusker D, et al (2007)
View Run	BOI2	Boi 2 gst control	McCusker D, et al (2007)
View Run	MLP2	#25 Asynchronous Prep3-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#21 Asynchronous Prep2-IMAC Phosphopeptide enrichment A1	Keck JM, et al. (2011)
View Run	MLP2	#32 Asynchronous Prep-No Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#31 Asynchronous Prep (Protease cleavage)	Keck JM, et al. (2011)
View Run	MLP2	#04 Alpha Factor Prep2	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	RCY1	RPS12	1	Unpublished Fields Lab Data
View Screen	VIK1	RPS12	1	Unpublished Fields Lab Data
View Screen	PTC4	RPS12	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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No microscopy data found in the PDR for this protein.

Protein Structure Data

[What does the above image mean?]
[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..143]	PSI-BLAST	24.69897	Spliceosomal 15.5kd protein

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
structural constituent of ribosome	7.52323416912124	bayes_pls_golite062009
structural molecule activity	6.97581143389173	bayes_pls_golite062009
RNA binding	3.17103797166833	bayes_pls_golite062009
nucleic acid binding	1.89156659324072	bayes_pls_golite062009
binding	1.31970199852267	bayes_pls_golite062009
	0.61812240381857	bayes_pls_golite062009
catalytic activity	0.264002643439785	bayes_pls_golite062009
mRNA binding	0.259807967594031	bayes_pls_golite062009
protein binding	0.128962984939985	bayes_pls_golite062009

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.98

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2