Protein Overview: HIR2

General Information:
Name(s) found:	HIR2 / YOR038C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6324612\|ref\|NP_014681.1\| Non-essential transcriptional corepressor involved in the cell cycle-regulated transcription of histone H2A, H2B, H3, and H4 genes; recruits Swi-Snf complexes to histone gene promoters; promotes heterochromatic gene silencing w [NCBI NR] pir\|\|S62177 HIR2 protein - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|1708201\|sp\|P32480.2\|HIR2_YEAST RecName: Full=Protein HIR2; AltName: Full=Histone transcription regulator 2 [NCBI NR] gi\|1420160\|emb\|CAA99228.1\| HIR2 [Saccharomyces cerevisiae] [NCBI NR] gi\|1041663\|emb\|CAA60757.1\| ORF OR26.31 [Saccharomyces cerevisiae] [NCBI NR] Protein HIR2 OS=Saccharomyces cerevisiae GN=HIR2 PE=1 SV=2 [Swiss-Prot] HIR2 Histone transcription regulator [mips] Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; recruits Swi-Snf complexes to histone gene promoters; promotes heterochromatic gene silencing with Asf1p [SGD] (P32480) Histone transcription regulator 2 [4932.FASTACCERV.txt] Chr XV from 405387-402760, reverse complement [hmr_yeast5_DM3-1_pombe.fasta] Chr XV from 402760-405387, reverse complement [yeastorf3.fasta] HIR2, Chr XV from 402760-405387, reverse complement [yeast_orfs2] HIR2 SGDID:S0005564, Chr XV from 405387-402760, reverse complement, Verified ORF [yeastflysequest.fasta] Hir2p [Saccharomyces cerevisiae]gi\|1041663\|emb\|CAA60757.1\| ORF OR26.31 [Saccharomyces cerevisiae]gi\|1420160\|emb\|CAA99228.1\| HIR2 [Saccharomyces cerevisiae]gi\|1708201\|sp\|P32480\|HIR2_YEAST Histone transcription regulator 2gi\|2131281\|pir\|\|S62177 HIR2 pro [nrdb_11152004_Chlamydomonas] HIR2 SGDID:S000005564, Chr XV from 405387-402760, reverse complement, Verified ORF, "Non-essential transcriptional corepressor involved in the cell cycle-regulated transcription of histone H2A, H2B, H3, and H4 genes; recruits Swi-Snf complexes to histone [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] HIR2 SGDID:S000005564, Chr XV from 405388-402761, reverse complement, Verified ORF, "Non-essential transcriptional corepressor involved in the cell cycle-regulated transcription of histone H2A, H2B, H3, and H4 genes; recruits Swi-Snf complexes to histone [yeast-contam.fasta] HIR2 SGDID:S000005564, Chr XV from 405388-402761, reverse complement, Verified ORF, "Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; recruits Swi-Snf complexes to histone gene promoters; prom [SGD_S-cerevisiae_na_08-19-2008_con_reversed.fasta] Non-essential transcriptional corepressor involved in the cell cycle-regulated transcription of histone H2A, H2B, H3, and H4 genes; recruits Swi-Snf complexes to histone gene promoters; promotes heterochromatic gene silencing with Asf1p; Hir2p [Saccharomy [nr-271106-contam.fasta] ORF OR26.31 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] HIR2 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Histone transcription regulator 2 [nr-271106-contam.fasta] HIR2 SGDID:S000005564, Chr XV from 405388-402761, reverse complement, Verified ORF, "Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; recruits Swi-Snf complexes to histone gene promoters; promotes heterochromatic gene silencing with Asf1p" [yeast-bovrabprots.fasta] HIR2 SGDID:S000005564, Chr XV from 405388-402761, reverse complement, Verified ORF, "Non-essential transcriptional corepressor involved in the cell cycle-regulated transcription of histone H2A, H2B, H3, and H4 genes; recruits Swi-Snf complexes to histone gene promoters; promotes heterochromatic gene silencing with Asf1p" [yeast-contam_custom.fasta] [chemostatdb.fasta] Protein HIR2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=HIR2 PE=1 SV=2 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] HIR2 SGDID:S000005564, Chr XV from 405388-402761, Genome Release 64-1-1, reverse complement, Verified ORF, "Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; recruits Swi-Snf complexes to histone gene promoters; promotes heterochromatic gene silencing with Asf1p" [yeast-030211-contam.fasta] HIR2 SGDID:S000005564, Chr XV from 405388-402761, Genome Release 64-2-1, reverse complement, Verified ORF, "Subunit of HIR nucleosome assembly complex; involved in regulation of histone gene transcription; recruits Swi-Snf complexes to histone gene promoters; promotes heterochromatic gene silencing with Asf1p; relocalizes to the cytosol in response to hypoxia" [20141208_orf_trans.fasta] YOR038C SGDID:S000005564, Chr XV from 405388-402761, Genome Release 64-1-1, reverse complement, Verified ORF, "Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; recruits Swi-Snf complexes to histone gene promoters; promotes heterochromatic gene silencing with Asf1p" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	875 amino acids
Gene Ontology:
Cellular Component:	nucleus [IDA] HIR complex [IDA]
Biological Process:	DNA replication-independent nucleosome assembly [IDA] regulation of transcription from RNA polymerase II promoter [IDA] RNA elongation from RNA polymerase II promoter [IGI]
Molecular Function:	transcription corepressor activity [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MRLLKYPLDI HNEQVNALAA LGPYIILAGS GGHVMAWRQQ QLVDTAFDRV MIKDLKPEVS  60
   61 FQVDQDTTGD IFFITGDLET LYIGSEHRLW GYSGWLCRDT NNINSVEKMN SKLLFECKSP 120
  121 STITDVKYDI NLGILFVLLS NENKILLFRH KTFDKLSEIT IDKASKPITG IIDPTGQTFT 180
  181 VMTSDRSILV YQINKTGTHK LINKLTQHVQ MYPLHYRISM SPQADILPVI NSVKGVPNNA 240
  241 TSCTALLDRN NNYKVTKTLV TPSSNGCRVL VYSPAFYEKP NLKKGTSTRY NLIATSGSTD 300
  301 GTILVWNTKR MKPLFNALQV SSTAINDMSW SQDGFTLFAI SNDATLYTFA FQEKDLGVAL 360
  361 PQTEIKSLQE VNKKLPKLEE PLAEQIPKSF PENIKLEESA SAAPIPNDIG RSAVGKKPTK 420
  421 KKTANNQTNG IKTIQSTSME FNTPSYTVPR DLKRKPKEAT PSNIAPGSKK QKKELQPIDF 480
  481 LDTGLLLPNT SFSRIRLATP KIRSTFKYSP INNPNLILDV KNGSGNEQRP TIVKLTSKVL 540
  541 DQDQVLFQDF IPKLITICTA GDTFWSFCSE DGSIYIYSDS GRKLMAPLVL GVSISFLEAC 600
  601 GTYLLCLTSI GELYCWNIEQ KKLAFPTNTI YPLLNPSLRY SDDILTRAEN ITLCSITKKG 660
  661 VPLVTLSNGD GYLFDKNMET WLLVSDGWWA YGSQYWDTTN TTGLSSSKAN TDSFNGSESN 720
  721 INEIVSDIKN DNQSIINFLE CKTNDELNRK GRIKNLQRFA RTILMKEGFE NMEEIVTLSH 780
  781 LENKILISIR LEEPEEFSKL MMVYCIRLSE LGYMDRLNDV FQWLYDDLPI SGTGSAFADK 840
  841 DFKRNLLKKI LIACGDIRQV QRVTTRYAKE MNIIS

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		ASF1 HIR1 HIR2 HIR3 HPC2 RAD53
View Details	Krogan NJ, et al. (2006)		ASF1 HIR1 HIR2 HIR3 HPC2 QDR2 SFP1
View Details	Ho Y, et al. (2002)		HHF1, HHF2 HIR2 HTA1 HTB1 KAP114 KRI1 NAP1 NOP4 NOP7 RET1 RPC82 RPO31 SPT16 UTP13
View Details	Qiu et al. (2008)		CAC2 HIR1 HIR2 HIR3 HPC2 MSI1 RLF2

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	HIR2	No Comments	Green EM, et al (2005)
View Run	ASF1	sample 3121 run on DecaLCQ: searched for phosphorylation	Green EM, et al (2005)
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	RLF2	phosphorylation data - cascade search	Green EM, et al (2005)
View Run	RLF2	identification data - first search in cascade	Green EM, et al (2005)
View Run	ASF1	sample 3928 - Asf1-S-TEV-ZZ (in hir3delta mutant)	Green EM, et al (2005)
View Run	MLP2	#31 Asynchronous Prep (Protease cleavage)	Keck JM, et al. (2011)
View Run	MLP2	#03 Alpha Factor Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#12 Mitotic Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	None Entered	#17 Mitotic Prep3-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	TOP3	HIR2	1	Unpublished Fields Lab Data
View Screen	UBC7	HIR2	1	Unpublished Fields Lab Data
View Screen	YOR203W	HIR2	1	Unpublished Fields Lab Data
View Screen	LSM8	HIR2	1	Unpublished Fields Lab Data

Microscopy / Localization Data

New Feature: Upload Your Own Microscopy Data

	PROTEIN(S)	PUBLICATION
View Data	HIR2	Huh WK, et al. (2003)
View Data	HIR2 and NOP56	Huh WK, et al. (2003)

Protein Structure Data

[What does the above image mean?]
[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..85]	MSA	1.652995	View MSA. No confident structure predictions are available.
2	View Details	[86..473]	PSI-BLAST	70.39794	beta1-subunit of the signal-transducing G protein heterotrimer
3	View Details	[474..698]	MSA	23.515997	View MSA. No confident structure predictions are available.
4	View Details	[699..752]	deduced	N/A	No confident structure predictions are available.
5	View Details	[753..875]	ORFEUS	7.63	5' to 3' exonuclease domain of DNA polymerase Taq; Exonuclease domain of prokaryotic DNA polymerase; DNA polymerase I (Klenow fragment)

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
general RNA polymerase II transcription factor activity	2.79445416141967	bayes_pls_golite062009
binding	2.42570478946173	bayes_pls_golite062009
transcription regulator activity	2.3610959773142	bayes_pls_golite062009
nucleic acid binding	2.30918780727915	bayes_pls_golite062009
transcription repressor activity	2.29049708962212	bayes_pls_golite062009
DNA binding	1.71685536470455	bayes_pls_golite062009
transcription corepressor activity	1.41752877005447	bayes_pls_golite062009
protein binding	1.21879977456466	bayes_pls_golite062009
cytoskeletal protein binding	1.19593750149343	bayes_pls_golite062009
transcription factor activity	1.08768505897883	bayes_pls_golite062009
	0.88172763425403	bayes_pls_golite062009
histone binding	0.685319917508843	bayes_pls_golite062009
RNA polymerase II transcription factor activity	0.676924760416604	bayes_pls_golite062009
actin binding	0.529861581509437	bayes_pls_golite062009
transcription factor binding	0.489473003915035	bayes_pls_golite062009
snoRNA binding	0.347797037104681	bayes_pls_golite062009
transcription cofactor activity	0.30761651528651	bayes_pls_golite062009
transporter activity	0.284701931503833	bayes_pls_golite062009
DNA-dependent ATPase activity	0.164101908612691	bayes_pls_golite062009
chromatin binding	0.151977098745772	bayes_pls_golite062009
structural molecule activity	0.0936365746095786	bayes_pls_golite062009

Term	Confidence	Notes
transcription regulator activity	5.23858959608048	bayes_pls_golite062009
DNA binding	4.78517210147551	bayes_pls_golite062009
nucleic acid binding	4.72059012764202	bayes_pls_golite062009
transcription factor activity	4.02736190679869	bayes_pls_golite062009
transcription repressor activity	3.60278632732466	bayes_pls_golite062009
binding	3.37660003833383	bayes_pls_golite062009
general RNA polymerase II transcription factor activity	2.84400558797599	bayes_pls_golite062009
RNA polymerase II transcription factor activity	2.41045867230388	bayes_pls_golite062009
sequence-specific DNA binding	2.21432668306508	bayes_pls_golite062009
transcription activator activity	2.13702350917359	bayes_pls_golite062009
transcription elongation regulator activity	1.75305601393584	bayes_pls_golite062009
structure-specific DNA binding	0.795220428630727	bayes_pls_golite062009
transcription factor binding	0.752335897765285	bayes_pls_golite062009
protein binding	0.726445167461826	bayes_pls_golite062009
specific transcriptional repressor activity	0.710995647413393	bayes_pls_golite062009
specific RNA polymerase II transcription factor activity	0.490809930581315	bayes_pls_golite062009
transcription corepressor activity	0.252392400929265	bayes_pls_golite062009
DNA-dependent ATPase activity	0.224230323813474	bayes_pls_golite062009
structural molecule activity	0.128347187436068	bayes_pls_golite062009
RNA polymerase II transcription factor activity, enhancer binding	0.0406721670764369	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

[View Details]

Source: Reynolds et al. 2008. Manuscript submitted

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2