Protein Overview: ORC1

General Information:
Name(s) found:	ORC1 / YML065W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6323575\|ref\|NP_013646.1\| Largest subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; exhibits ATPase activity; Orc1p [Saccharomyces cerevisiae] [NCBI NR] pir\|\|S48333 ORC1 protein - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|558410\|emb\|CAA86256.1\| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR] gi\|1709488\|sp\|P54784.1\|ORC1_YEAST RecName: Full=Origin recognition complex subunit 1; AltName: Full=Origin recognition complex 120 kDa subunit [NCBI NR] gi\|1065911\|gb\|AAB38248.1\| Orc1p [Saccharomyces cerevisiae] [NCBI NR] Origin recognition complex subunit 1 OS=Saccharomyces cerevisiae GN=ORC1 PE=1 SV=1 [Swiss-Prot] ORC1 Origin recognition complex, 104 KD subunit [mips] Largest subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; exhibits ATPase activity [SGD] ORC1, Chr XIII from 142210-144954 [yeast_orfs2] Chr XIII from 142210-144954 [yeastorf3.fasta] (P54784) Origin recognition complex subunit 1 (Origin recognition complex protein 120 kDa subunit) [4932.FASTACCERV.txt] ORC1 SGDID:S0004530, Chr XIII from 142210-144954, Verified ORF [yeastflysequest.fasta] Largest subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; may be phosphorylated by Cdc28p [Saccharomyces cerevisiae]gi\|558410\|emb\|CAA86256.1\| unna [nrdb_11152004_Chlamydomonas] ORC1 SGDID:S000004530, Chr XIII from 142210-144954, Verified ORF, "Largest subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; may be phosphorylated [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] ORC1 SGDID:S000004530, Chr XIII from 142210-144954, Verified ORF, "Largest subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; exhibits ATPase activi [SGD_S-cerevisiae_na_08-19-2008_con_reversed.fasta] unnamed protein product [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Origin recognition complex subunit 1 (Origin recognition complex 120 kDa subunit) [nr-271106-contam.fasta] Orc1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Largest subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; may be phosphorylated by Cdc28p; Orc1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] ORC1 SGDID:S000004530, Chr XIII from 142210-144954, Verified ORF, "Largest subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; exhibits ATPase activity" [yeast-bovrabprots.fasta] ORC1 SGDID:S000004530, Chr XIII from 142210-144954, Verified ORF, "Largest subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; may be phosphorylated by Cdc28p" [yeast-contam_custom.fasta] [chemostatdb.fasta] Origin recognition complex subunit 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=ORC1 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] ORC1 SGDID:S000004530, Chr XIII from 142210-144954, Genome Release 64-1-1, Verified ORF, "Largest subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; exhibits ATPase activity" [yeast-030211-contam.fasta] ORC1 SGDID:S000004530, Chr XIII from 142210-144954, Genome Release 64-2-1, Verified ORF, "Largest subunit of the origin recognition complex; involved in directing DNA replication by binding to replication origins; also involved in transcriptional silencing; exhibits ATPase activity; ORC1 has a paralog, SIR3, that arose from the whole genome duplication" [20141208_orf_trans.fasta] YML065W SGDID:S000004530, Chr XIII from 142210-144954, Genome Release 64-1-1, Verified ORF, "Largest subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; exhibits ATPase activity" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	914 amino acids
Gene Ontology:
Cellular Component:	pre-replicative complex [TAS] nuclear origin of replication recognition complex [IDA][IMP]
Biological Process:	DNA replication initiation [TAS] pre-replicative complex assembly [TAS] chromatin silencing at silent mating-type cassette [TAS]
Molecular Function:	ATPase activity [TAS] DNA replication origin binding [TAS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MAKTLKDLQG WEIITTDEQG NIIDGGQKRL RRRGAKTEHY LKRSSDGIKL GRGDSVVMHN  60
   61 EAAGTYSVYM IQELRLNTLN NVVELWALTY LRWFEVNPLA HYRQFNPDAN ILNRPLNYYN 120
  121 KLFSETANKN ELYLTAELAE LQLFNFIRVA NVMDGSKWEV LKGNVDPERD FTVRYICEPT 180
  181 GEKFVDINIE DVKAYIKKVE PREAQEYLKD LTLPSKKKEI KRGPQKKDKA TQTAQISDAE 240
  241 TRATDITDNE DGNEDESSDY ESPSDIDVSE DMDSGEISAD ELEEEEDEEE DEDEEEKEAR 300
  301 HTNSPRKRGR KIKLGKDDID ASVQPPPKKR GRKPKDPSKP RQMLLISSCR ANNTPVIRKF 360
  361 TKKNVARAKK KYTPFSKRFK SIAAIPDLTS LPEFYGNSSE LMASRFENKL KTTQKHQIVE 420
  421 TIFSKVKKQL NSSYVKEEIL KSANFQDYLP ARENEFASIY LSAYSAIESD SATTIYVAGT 480
  481 PGVGKTLTVR EVVKELLSSS AQREIPDFLY VEINGLKMVK PTDCYETLWN KVSGERLTWA 540
  541 ASMESLEFYF KRVPKNKKKT IVVLLDELDA MVTKSQDIMY NFFNWTTYEN AKLIVIAVAN 600
  601 TMDLPERQLG NKITSRIGFT RIMFTGYTHE ELKNIIDLRL KGLNDSFFYV DTKTGNAILI 660
  661 DAAGNDTTVK QTLPEDVRKV RLRMSADAIE IASRKVASVS GDARRALKVC KRAAEIAEKH 720
  721 YMAKHGYGYD GKTVIEDENE EQIYDDEDKD LIESNKAKDD NDDDDDNDGV QTVHITHVMK 780
  781 ALNETLNSHV ITFMTRLSFT AKLFIYALLN LMKKNGSQEQ ELGDIVDEIK LLIEVNGSNK 840
  841 FVMEIAKTLF QQGSDNISEQ LRIISWDFVL NQLLDAGILF KQTMKNDRIC CVKLNISVEE 900
  901 AKRAMNEDET LRNL

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		HAT1 HAT2 ORC1 ORC2 ORC3 ORC4 ORC5 ORC6
View Details	(MIPS) Mewes HW, et al. (2004)		CDC45 CDC6 CDC7 DBF4 DPB11 DPB2 DPB3 MCM2 MCM3 MCM4 MCM5 MCM6 MCM7 ORC1 ORC2 ORC3 ORC4 ORC5 ORC6 POL2 POL3 POL31 POL32 SLD3
View Details	Krogan NJ, et al. (2006)		HAT1 HAT2 ORC1 ORC2 ORC3 ORC4 ORC5 ORC6 SWE1 YJR154W
View Details	Gavin AC, et al. (2006)		ORC1 ORC2 ORC3 ORC4 ORC5 ORC6
View Details	Gavin AC, et al. (2002)		CDC45 ERG26 GLT1 HAT1 HSP42 LYS12 MCM2 MCM5 MCM6 MCM7 ORC1 ORC2 ORC3 ORC4 ORC5 ORC6 RPD3 RPN9 SIN3 TFP1
View Details	Qiu et al. (2008)		CDC45 CDC6 DBF4 DPB11 MCM2 MCM3 MCM4 MCM5 MCM6 MCM7 ORC1 ORC2 ORC3 ORC4 ORC5 ORC6 POL2 POL31 POL32 SLD3

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	MLP2	#26 Asynchronous Prep4-TiO2 Phosphopeptide enriched, Steps1-2	Keck JM, et al. (2011)
View Run	MLP2	#03 Alpha Factor Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#12 Mitotic Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#11 Mitotic Prep1-TiO2 enriched, new search criteria	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	BUR2	ORC1	2	Unpublished Fields Lab Data
View Screen	RAD1	ORC1	2	Unpublished Fields Lab Data

Microscopy / Localization Data

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No microscopy data found in the PDR for this protein.

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..198]	Pfam	25.045757	BAH domain No confident structure predictions are available.
2	View Details	[199..325]	MSA	2.482996	View MSA. No confident structure predictions are available.
3	View Details	[326..409]	deduced	N/A	No confident structure predictions are available.
4	View Details	[410..626]	PSI-BLAST	51.39794	Membrane fusion atpase p97 N-terminal domain , P97-Nn; Membrane fusion atpase p97, D1 domain; Membrane fusion atpase p97 domain 2, P97-Nc
5	View Details	[627..758]	PSI-BLAST	51.39794	Membrane fusion atpase p97 N-terminal domain , P97-Nn; Membrane fusion atpase p97, D1 domain; Membrane fusion atpase p97 domain 2, P97-Nc
6	View Details	[759..914]	deduced	N/A	No confident structure predictions are available.

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
structural constituent of chromatin	5.63555259413678	bayes_pls_golite062009
DNA replication origin binding	4.6882205228007	bayes_pls_golite062009
nucleosomal histone binding	4.23077815319444	bayes_pls_golite062009
nucleosome binding	3.68770692323111	bayes_pls_golite062009
DNA binding	3.49504261357874	bayes_pls_golite062009
nucleic acid binding	3.45109302518995	bayes_pls_golite062009
chromatin binding	2.76371060113086	bayes_pls_golite062009
binding	2.47268310088535	bayes_pls_golite062009
structure-specific DNA binding	2.06404194869726	bayes_pls_golite062009
single-stranded DNA binding	1.99705816692751	bayes_pls_golite062009
double-stranded DNA binding	1.92992435554938	bayes_pls_golite062009
sequence-specific DNA binding	1.53908742265394	bayes_pls_golite062009
structural molecule activity	1.23840733283098	bayes_pls_golite062009
histone binding	0.8857281851882	bayes_pls_golite062009
protein binding	0.634982583867058	bayes_pls_golite062009
catalytic activity	0.424959044318554	bayes_pls_golite062009
protein dimerization activity	0.0647375627009996	bayes_pls_golite062009

Term	Confidence	Notes
DNA clamp loader activity	3.22518920754305	bayes_pls_golite062009
protein-DNA loading ATPase activity	3.19273435123154	bayes_pls_golite062009
DNA-dependent ATPase activity	2.72462789867829	bayes_pls_golite062009
general RNA polymerase II transcription factor activity	2.66107861968804	bayes_pls_golite062009
binding	2.64537357529343	bayes_pls_golite062009
nucleic acid binding	2.51683451033161	bayes_pls_golite062009
DNA helicase activity	2.2817164193758	bayes_pls_golite062009
pyrophosphatase activity	2.13284212711072	bayes_pls_golite062009
DNA binding	2.12258000781149	bayes_pls_golite062009
transcription regulator activity	2.09345198747025	bayes_pls_golite062009
ATP-dependent DNA helicase activity	1.88455339138556	bayes_pls_golite062009
helicase activity	1.70242731830224	bayes_pls_golite062009
RNA helicase activity	1.63960194106079	bayes_pls_golite062009
DNA replication origin binding	1.62491387774159	bayes_pls_golite062009
ATP-dependent RNA helicase activity	1.61735400124552	bayes_pls_golite062009
RNA-dependent ATPase activity	1.60404584546501	bayes_pls_golite062009
purine NTP-dependent helicase activity	1.19311444180077	bayes_pls_golite062009
ATP-dependent helicase activity	1.19311444180077	bayes_pls_golite062009
catalytic activity	1.00104173493322	bayes_pls_golite062009
DNA-directed DNA polymerase activity	0.971476020912368	bayes_pls_golite062009
DNA polymerase activity	0.928091843881235	bayes_pls_golite062009
transcription factor activity	0.887108427603557	bayes_pls_golite062009
transcription repressor activity	0.862685397469217	bayes_pls_golite062009
structure-specific DNA binding	0.782430219754236	bayes_pls_golite062009
motor activity	0.61636596887462	bayes_pls_golite062009
double-stranded DNA binding	0.6071273686445	bayes_pls_golite062009
protein binding	0.170837278876362	bayes_pls_golite062009
nucleoside-triphosphatase activity	0.147579541298736	bayes_pls_golite062009
nucleotide binding	0.114848452385726	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides	0.113504049290503	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	0.111293884322157	bayes_pls_golite062009
purine nucleotide binding	0.10713474095071	bayes_pls_golite062009
purine ribonucleotide binding	0.0916519414118148	bayes_pls_golite062009
ribonucleotide binding	0.0915913646217636	bayes_pls_golite062009
structural constituent of ribosome	0.089234257580272	bayes_pls_golite062009
3'-5' DNA helicase activity	0.0769112340058804	bayes_pls_golite062009
cytoskeletal protein binding	0.0272673919333979	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.97

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2