Protein Overview: STE6

General Information:
Name(s) found:	STE6 / YKL209C [SGD]
Description(s) found: SHOW ONLY BEST	pir\|\|DVBYS6 mating pheromone a secretion protein STE6 - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|6322640\|ref\|NP_012713.1\| Plasma membrane ATP-binding cassette (ABC) transporter required for the export of a-factor, catalyzes ATP hydrolysis coupled to a-factor transport; contains 12 transmembrane domains and two ATP binding domains; expressed only i [NCBI NR] gi\|486373\|emb\|CAA82054.1\| STE6 [Saccharomyces cerevisiae] [NCBI NR] gi\|4564\|emb\|CAA33467.1\| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR] gi\|172758\|gb\|AAA35116.1\| Ste6 protein [NCBI NR] gi\|134967\|sp\|P12866.1\|STE6_YEAST RecName: Full=Alpha-factor-transporting ATPase; AltName: Full=Mating factor A secretion protein STE6; AltName: Full=Multiple drug resistance protein homolog; AltName: Full=P-glycoprotein [NCBI NR] Alpha-factor-transporting ATPase OS=Saccharomyces cerevisiae GN=STE6 PE=1 SV=1 [Swiss-Prot] STE6 Full-size ABC transporter responsible for export of the a factor mating pheromone [mips] Plasma membrane ATP-binding cassette (ABC) transporter required for the export of a-factor, catalyzes ATP hydrolysis coupled to a-factor transport; contains 12 transmembrane domains and two ATP binding domains; expressed only in MATa cells [SGD] STE6, Chr XI from 42424-46296, reverse complement [yeast_orfs2] Chr XI from 42424-46296, reverse complement [yeastorf3.fasta] Chr XI from 46296-42424, reverse complement [yeastorf5.fasta] (P12866) Mating factor A secretion protein STE6 (Multiple drug resistance protein homolog) (P-glycoprotein) [4932.FASTACCERV.txt] STE6 SGDID:S0001692, Chr XI from 46296-42424, reverse complement, Verified ORF [yeastflysequest.fasta] ABC transporter, glycoprotein, component of a-factor secretory pathway [Saccharomyces cerevisiae]gi\|486373\|emb\|CAA82054.1\| STE6 [Saccharomyces cerevisiae]gi\|4564\|emb\|CAA33467.1\| unnamed protein product [Saccharomyces cerevisiae]gi\|72480\|pir\|\|DVBYS6 mat [nrdb_11152004_Chlamydomonas] STE6 SGDID:S000001692, Chr XI from 46296-42424, reverse complement, Verified ORF, "ATP-binding cassette (ABC) transporter required for the export of a-factor, catalyzes ATP hydrolysis coupled to a-factor transport, contains 12 transmembrane domains and tw [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] STE6 SGDID:S000001692, Chr XI from 46296-42424, reverse complement, Verified ORF, "Plasma membrane ATP-binding cassette (ABC) transporter required for the export of a-factor, catalyzes ATP hydrolysis coupled to a-factor transport; contains 12 transmembran [SGD_S-cerevisiae_na_08-19-2008_con_reversed.fasta] ATP-binding cassette (ABC) transporter required for the export of a-factor, catalyzes ATP hydrolysis coupled to a-factor transport, contains 12 transmembrane domains and two ATP binding domains, expressed only in MATa cells; Ste6p [Saccharomyces cerevisia [nr-271106-contam.fasta] Ste6 protein [nr-271106-contam.fasta] unnamed protein product [Saccharomyces cerevisiae] [nr-271106-contam.fasta] STE6 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Mating factor A secretion protein STE6 (Multiple drug resistance protein homolog) (P-glycoprotein) [nr-271106-contam.fasta] STE6 SGDID:S000001692, Chr XI from 46296-42424, reverse complement, Verified ORF, "Plasma membrane ATP-binding cassette (ABC) transporter required for the export of a-factor, catalyzes ATP hydrolysis coupled to a-factor transport; contains 12 transmembrane domains and two ATP binding domains; expressed only in MATa cells" [yeast-bovrabprots.fasta] STE6 SGDID:S000001692, Chr XI from 46296-42424, reverse complement, Verified ORF, "ATP-binding cassette (ABC) transporter required for the export of a-factor, catalyzes ATP hydrolysis coupled to a-factor transport, contains 12 transmembrane domains and two ATP binding domains, expressed only in MATa cells" [yeast-contam_custom.fasta] [chemostatdb.fasta] Alpha-factor-transporting ATPase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=STE6 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] STE6 SGDID:S000001692, Chr XI from 46295-42423, Genome Release 64-1-1, reverse complement, Verified ORF, "Plasma membrane ATP-binding cassette (ABC) transporter required for the export of a-factor, catalyzes ATP hydrolysis coupled to a-factor transport; contains 12 transmembrane domains and two ATP binding domains; expressed only in MATa cells" [yeast-030211-contam.fasta] STE6 SGDID:S000001692, Chr XI from 46295-42423, Genome Release 64-2-1, reverse complement, Verified ORF, "Plasma membrane ATP-binding cassette (ABC) transporter; required for the export of a-factor, catalyzes ATP hydrolysis coupled to a-factor transport; contains 12 transmembrane domains and two ATP binding domains; expressed only in MATa cells" [20141208_orf_trans.fasta] YKL209C SGDID:S000001692, Chr XI from 46295-42423, Genome Release 64-1-1, reverse complement, Verified ORF, "Plasma membrane ATP-binding cassette (ABC) transporter required for the export of a-factor, catalyzes ATP hydrolysis coupled to a-factor transport; contains 12 transmembrane domains and two ATP binding domains; expressed only in MATa cells" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	1290 amino acids
Gene Ontology:
Cellular Component:	integral to plasma membrane [TAS] mating projection tip [IDA]
Biological Process:	peptide pheromone export [TAS]
Molecular Function:	ATPase activity, coupled to transmembrane movement of substances [IDA][TAS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MNFLSFKTTK HYHIFRYVNI RNDYRLLMIM IIGTVATGLV PAITSILTGR VFDLLSVFVA  60
   61 NGSHQGLYSQ LVQRSMAVMA LGAASVPVMW LSLTSWMHIG ERQGFRIRSQ ILEAYLEEKP 120
  121 MEWYDNNEKL LGDFTQINRC VEELRSSSAE ASAITFQNLV AICALLGTSF YYSWSLTLII 180
  181 LCSSPIITFF AVVFSRMIHV YSEKENSETS KAAQLLTWSM NAAQLVRLYC TQRLERKKFK 240
  241 EIILNCNTFF IKSCFFVAAN AGILRFLTLT MFVQGFWFGS AMIKKGKLNI NDVITCFHSC 300
  301 IMLGSTLNNT LHQIVVLQKG GVAMEKIMTL LKDGSKRNPL NKTVAHQFPL DYATSDLTFA 360
  361 NVSFSYPSRP SEAVLKNVSL NFSAGQFTFI VGKSGSGKST LSNLLLRFYD GYNGSISING 420
  421 HNIQTIDQKL LIENITVVEQ RCTLFNDTLR KNILLGSTDS VRNADCSTNE NRHLIKDACQ 480
  481 MALLDRFILD LPDGLETLIG TGGVTLSGGQ QQRVAIARAF IRDTPILFLD EAVSALDIVH 540
  541 RNLLMKAIRH WRKGKTTIIL THELSQIESD DYLYLMKEGE VVESGTQSEL LADPTTTFST 600
  601 WYHLQNDYSD AKTIVDTETE EKSIHTVESF NSQLETPKLG SCLSNLGYDE TDQLSFYEAI 660
  661 YQKRSNVRTR RVKVEEENIG YALKQQKNTE SSTGPQLLSI IQIIKRMIKS IRYKKILILG 720
  721 LLCSLIAGAT NPVFSYTFSF LLEGIVPSTD GKTGSSHYLA KWSLLVLGVA AADGIFNFAK 780
  781 GFLLDCCSEY WVMDLRNEVM EKLTRKNMDW FSGENNKASE ISALVLNDLR DLRSLVSEFL 840
  841 SAMTSFVTVS TIGLIWALVS GWKLSLVCIS MFPLIIIFSA IYGGILQKCE TDYKTSVAQL 900
  901 ENCLYQIVTN IKTIKCLQAE FHFQLTYHDL KIKMQQIASK RAIATGFGIS MTNMIVMCIQ 960
  961 AIIYYYGLKL VMIHEYTSKE MFTTFTLLLF TIMSCTSLVS QIPDISRGQR AASWIYRILD1020
 1021 EKHNTLEVEN NNARTVGIAG HTYHGKEKKP IVSIQNLTFA YPSAPTAFVY KNMNFDMFCG1080
 1081 QTLGIIGESG TGKSTLVLLL TKLYNCEVGK IKIDGTDVND WNLTSLRKEI SVVEQKPLLF1140
 1141 NGTIRDNLTY GLQDEILEIE MYDALKYVGI HDFVISSPQG LDTRIDTTLL SGGQAQRLCI1200
 1201 ARALLRKSKI LILDECTSAL DSVSSSIINE IVKKGPPALL TMVITHSEQM MRSCNSIAVL1260
 1261 KDGKVVERGN FDTLYNNRGE LFQIVSNQSS

Protein Complex Data

No complex found for this protein.

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	TOR1	No Comments	Reinke A, et al. (2004)
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	RLF2	phosphorylation data - cascade search	Green EM, et al (2005)
View Run	MLP2	#03 Alpha Factor Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#12 Mitotic Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#11 Mitotic Prep1-TiO2 enriched, new search criteria	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	UBC6	STE6	1	Unpublished Fields Lab Data
View Screen	PRP16	STE6	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	STE6	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..120]	ORFEUS	10.84	Heavy meromyosin subfragment
2	View Details	[121..343]	ORFEUS	10.84	Heavy meromyosin subfragment
3	View Details	[344..639]	PSI-BLAST	198.045757	MJ0796
4	View Details	[640..695]	deduced	N/A	No confident structure predictions are available.
5	View Details	[696..802] [823..1020]	PSI-BLAST	711.9794	MsbA
6	View Details	[803..822] [1021..1290]	PSI-BLAST	711.9794	MsbA

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
lipid transporter activity	6.95325922226092	bayes_pls_golite062009
ATPase activity, coupled to transmembrane movement of substances	5.4467861893359	bayes_pls_golite062009
ATPase activity, coupled to movement of substances	5.44654566127945	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances	5.44654566127945	bayes_pls_golite062009
P-P-bond-hydrolysis-driven transmembrane transporter activity	5.43909190876515	bayes_pls_golite062009
primary active transmembrane transporter activity	5.4304944906989	bayes_pls_golite062009
active transmembrane transporter activity	5.38112407181712	bayes_pls_golite062009
ATPase activity, coupled	4.87869064889244	bayes_pls_golite062009
ATPase activity	4.60773943958528	bayes_pls_golite062009
nucleoside-triphosphatase activity	4.38591214726007	bayes_pls_golite062009
pyrophosphatase activity	4.32673473878039	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides	4.31087891816858	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	4.31037981322306	bayes_pls_golite062009
transporter activity	3.47953169973151	bayes_pls_golite062009
ATP binding	3.44940587603537	bayes_pls_golite062009
adenyl ribonucleotide binding	3.39720556954135	bayes_pls_golite062009
adenyl nucleotide binding	3.3407335909134	bayes_pls_golite062009
hydrolase activity	3.11706952898443	bayes_pls_golite062009
substrate-specific transmembrane transporter activity	2.96171085039592	bayes_pls_golite062009
ribonucleotide binding	2.94542170749201	bayes_pls_golite062009
purine ribonucleotide binding	2.94377266066642	bayes_pls_golite062009
transmembrane transporter activity	2.94175800357966	bayes_pls_golite062009
substrate-specific transporter activity	2.90743799425416	bayes_pls_golite062009
purine nucleotide binding	2.89330081076061	bayes_pls_golite062009
nucleotide binding	2.86779390539461	bayes_pls_golite062009
binding	1.37806419883931	bayes_pls_golite062009
carbohydrate transmembrane transporter activity	0.989925244519283	bayes_pls_golite062009
catalytic activity	0.372934161904337	bayes_pls_golite062009

No functions predicted.

Term	Confidence	Notes
lipid transporter activity	7.47642599298853	bayes_pls_golite062009
ATPase activity, coupled to transmembrane movement of substances	5.57844095962502	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances	5.57813579764186	bayes_pls_golite062009
ATPase activity, coupled to movement of substances	5.57813579764186	bayes_pls_golite062009
P-P-bond-hydrolysis-driven transmembrane transporter activity	5.5689620139321	bayes_pls_golite062009
primary active transmembrane transporter activity	5.55992639965745	bayes_pls_golite062009
active transmembrane transporter activity	5.50005167063544	bayes_pls_golite062009
ATPase activity, coupled	4.98276715440477	bayes_pls_golite062009
heme transporter activity	4.82479229680991	bayes_pls_golite062009
peptide transporter activity	4.7442815698413	bayes_pls_golite062009
anion transmembrane-transporting ATPase activity	4.71935767060308	bayes_pls_golite062009
ATPase activity	4.69795387297193	bayes_pls_golite062009
peptide-transporting ATPase activity	4.63590351019108	bayes_pls_golite062009
nucleoside-triphosphatase activity	4.45923622153365	bayes_pls_golite062009
pyrophosphatase activity	4.39781350157199	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides	4.38133275795019	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	4.38085303582726	bayes_pls_golite062009
cofactor transporter activity	4.34709589308604	bayes_pls_golite062009
oligopeptide-transporting ATPase activity	4.09365342567844	bayes_pls_golite062009
transporter activity	3.71239480053329	bayes_pls_golite062009
ATP binding	3.62300973398915	bayes_pls_golite062009
hydrogen ion transporting ATP synthase activity, rotational mechanism	3.57926427233638	bayes_pls_golite062009
adenyl ribonucleotide binding	3.56796420118589	bayes_pls_golite062009
adenyl nucleotide binding	3.50845581803587	bayes_pls_golite062009
ion transmembrane transporter activity	3.35436118751376	bayes_pls_golite062009
phosphate transmembrane-transporting ATPase activity	3.20894563245332	bayes_pls_golite062009
inorganic anion transmembrane transporter activity	3.16975154423199	bayes_pls_golite062009
hydrolase activity	3.14498892233323	bayes_pls_golite062009
transmembrane transporter activity	3.13960906549884	bayes_pls_golite062009
anion transmembrane transporter activity	3.13913638619234	bayes_pls_golite062009
substrate-specific transporter activity	3.1318898376276	bayes_pls_golite062009
ribonucleotide binding	3.09294049852753	bayes_pls_golite062009
purine ribonucleotide binding	3.09132855853944	bayes_pls_golite062009
cation transmembrane transporter activity	3.08390071341252	bayes_pls_golite062009
iron ion transmembrane transporter activity	3.05511942104215	bayes_pls_golite062009
purine nucleotide binding	3.03820055021242	bayes_pls_golite062009
nucleotide binding	3.0109684031543	bayes_pls_golite062009
substrate-specific transmembrane transporter activity	3.00745742755195	bayes_pls_golite062009
drug transmembrane transporter activity	2.97119034312001	bayes_pls_golite062009
monovalent inorganic cation transmembrane transporter activity	2.9271291809464	bayes_pls_golite062009
branched-chain aliphatic amino acid transmembrane transporter activity	2.88795687640992	bayes_pls_golite062009
metal ion transmembrane transporter activity	2.88476360771975	bayes_pls_golite062009
amine-transporting ATPase activity	2.69529191205818	bayes_pls_golite062009
organic acid transmembrane transporter activity	2.67957846350199	bayes_pls_golite062009
carboxylic acid transmembrane transporter activity	2.67815672695824	bayes_pls_golite062009
hydrogen ion transmembrane transporter activity	2.63419811368692	bayes_pls_golite062009
sterol transporter activity	2.48473697371886	bayes_pls_golite062009
inorganic cation transmembrane transporter activity	2.40292717311506	bayes_pls_golite062009
canalicular bile acid transmembrane transporter activity	2.33958437214304	bayes_pls_golite062009
amine transmembrane transporter activity	2.29486577878686	bayes_pls_golite062009
	2.20174656498665	bayes_pls_golite062009
phosphate transmembrane transporter activity	2.15147988861177	bayes_pls_golite062009
oligopeptide transporter activity	2.05580564974083	bayes_pls_golite062009
transition metal ion transmembrane transporter activity	1.82177108193332	bayes_pls_golite062009
organic anion transmembrane transporter activity	1.76999970080179	bayes_pls_golite062009
protein transporter activity	1.71214667007262	bayes_pls_golite062009
potassium channel activity	1.68725597823541	bayes_pls_golite062009
amino acid transmembrane transporter activity	1.59627719545436	bayes_pls_golite062009
sugar transmembrane transporter activity	1.57734504779864	bayes_pls_golite062009
binding	1.40438143932554	bayes_pls_golite062009
phosphonate transmembrane transporter activity	1.40269234722336	bayes_pls_golite062009
carbohydrate-transporting ATPase activity	1.37859980472197	bayes_pls_golite062009
monocarboxylic acid transmembrane transporter activity	1.37836402136387	bayes_pls_golite062009
manganese ion transmembrane transporter activity	1.34412762758893	bayes_pls_golite062009
cation channel activity	1.33585529285301	bayes_pls_golite062009
molybdate ion transmembrane transporter activity	1.28313088935613	bayes_pls_golite062009
molybdate transmembrane-transporting ATPase activity	1.28313088935613	bayes_pls_golite062009
carbohydrate-importing ATPase activity	1.27327946933978	bayes_pls_golite062009
ATPase activity, coupled to transmembrane movement of ions	1.23799860254751	bayes_pls_golite062009
carbohydrate transmembrane transporter activity	1.21226071573355	bayes_pls_golite062009
sulfate transmembrane-transporting ATPase activity	1.1220946941463	bayes_pls_golite062009
manganese-transporting ATPase activity	1.05250190312265	bayes_pls_golite062009
dipeptide transporter activity	1.01130176523309	bayes_pls_golite062009
voltage-gated cation channel activity	1.00384984515681	bayes_pls_golite062009
voltage-gated ion channel activity	0.889208591820696	bayes_pls_golite062009
motor activity	0.87979065815418	bayes_pls_golite062009
voltage-gated channel activity	0.871525745291603	bayes_pls_golite062009
ion channel activity	0.848261056209476	bayes_pls_golite062009
GTPase activity	0.83849854735032	bayes_pls_golite062009
channel activity	0.806331380134838	bayes_pls_golite062009
passive transmembrane transporter activity	0.806331380134838	bayes_pls_golite062009
substrate-specific channel activity	0.799997700906738	bayes_pls_golite062009
copper ion transmembrane transporter activity	0.798934081014025	bayes_pls_golite062009
di-, tri-valent inorganic cation transmembrane transporter activity	0.767424210804186	bayes_pls_golite062009
bile acid transmembrane transporter activity	0.687612064139871	bayes_pls_golite062009
gated channel activity	0.650050847753837	bayes_pls_golite062009
cholesterol transporter activity	0.644421039250979	bayes_pls_golite062009
guanyl nucleotide binding	0.640772201693186	bayes_pls_golite062009
guanyl ribonucleotide binding	0.63468044350616	bayes_pls_golite062009
GTP binding	0.621689261700018	bayes_pls_golite062009
vitamin transporter activity	0.477646469354653	bayes_pls_golite062009
protein transmembrane transporter activity	0.46552249844591	bayes_pls_golite062009
catalytic activity	0.368034275052766	bayes_pls_golite062009
glucoside transmembrane transporter activity	0.31429820040527	bayes_pls_golite062009
maltose transmembrane transporter activity	0.309333293394255	bayes_pls_golite062009
alpha-glucoside transmembrane transporter activity	0.309333293394255	bayes_pls_golite062009
disaccharide transmembrane transporter activity	0.309333293394255	bayes_pls_golite062009
oligosaccharide transmembrane transporter activity	0.304521826432814	bayes_pls_golite062009
oligosaccharide-transporting ATPase activity	0.304521826432814	bayes_pls_golite062009
maltose-transporting ATPase activity	0.304521826432814	bayes_pls_golite062009
kinase activity	0.300431561463583	bayes_pls_golite062009
neutral amino acid transmembrane transporter activity	0.227570438294493	bayes_pls_golite062009
proton-transporting ATPase activity, rotational mechanism	0.219473333671469	bayes_pls_golite062009
signal recognition particle binding	0.20866768220789	bayes_pls_golite062009
protein kinase activity	0.194459978373898	bayes_pls_golite062009
protein binding	0.193768631623192	bayes_pls_golite062009
amino acid-transporting ATPase activity	0.147644650870465	bayes_pls_golite062009
nucleic acid binding	0.144904409289829	bayes_pls_golite062009
sulfate transmembrane transporter activity	0.104477131120205	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	0.0689844878028699	bayes_pls_golite062009
cation-transporting ATPase activity	0.0536898657672154	bayes_pls_golite062009
P-P-bond-hydrolysis-driven protein transmembrane transporter activity	0.00571427458217011	bayes_pls_golite062009
macromolecule transmembrane transporter activity	0.00571427458217011	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

[View Details]

Source: Reynolds et al. 2008. Manuscript submitted

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2