Protein Overview: MUD2

General Information:
Name(s) found:	MUD2 / YKL074C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6322776\|ref\|NP_012849.1\| Protein involved in early pre-mRNA splicing; component of the pre-mRNA-U1 snRNP complex, the commitment complex; interacts with Msl5p/BBP splicing factor and Sub2p; similar to metazoan splicing factor U2AF65; Mud2p [Saccharomyc [NCBI NR] pir\|\|S37899 hypothetical protein YKL074c - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|51338774\|sp\|P36084.3\|MUD2_YEAST RecName: Full=Splicing factor MUD2 [NCBI NR] gi\|486105\|emb\|CAA81911.1\| MUD2 [Saccharomyces cerevisiae] [NCBI NR] Splicing factor MUD2 OS=Saccharomyces cerevisiae GN=MUD2 PE=1 SV=3 [Swiss-Prot] MUD2 Pre-mRNA splicing factor [mips] Protein involved in early pre-mRNA splicing; component of the pre-mRNA-U1 snRNP complex, the commitment complex; interacts with Msl5p/BBP splicing factor and Sub2p; similar to metazoan splicing factor U2AF65 [SGD] MUD2, Chr XI from 294252-295835, reverse complement [yeast_orfs2] Chr XI from 294252-295835, reverse complement [yeastorf3.fasta] Chr XI from 294251-295834, reverse complement [yeastorf4.fasta] Chr XI from 295834-294251, reverse complement [yeastorf5.fasta] MUD2 SGDID:S0001557, Chr XI from 295838-294255, reverse complement, Verified ORF [yeastflysequest.fasta] Mud2p [Saccharomyces cerevisiae]gi\|486105\|emb\|CAA81911.1\| MUD2 [Saccharomyces cerevisiae]gi\|539177\|pir\|\|S37899 hypothetical protein YKL074c - yeast (Saccharomyces cerevisiae)gi\|51338774\|sp\|P36084\|MUD2_YEAST Splicing factor MUD2 [nrdb_11152004_Chlamydomonas] (P36084) Splicing factor MUD2 [cerv4932.FASTAC] MUD2 SGDID:S000001557, Chr XI from 295837-294254, reverse complement, Verified ORF, "Protein involved in early pre-mRNA splicing; component of the pre-mRNA-U1 snRNP complex, the commitment complex; interacts with Msl5p/BBP splicing factor and Sub2p; simil [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] Protein involved in early pre-mRNA splicing; component of the pre-mRNA-U1 snRNP complex, the commitment complex; interacts with Msl5p/BBP splicing factor and Sub2p; similar to metazoan splicing factor U2AF65; Mud2p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Splicing factor MUD2 [nr-271106-contam.fasta] MUD2 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] MUD2 SGDID:S000001557, Chr XI from 295837-294254, reverse complement, Verified ORF, "Protein involved in early pre-mRNA splicing; component of the pre-mRNA-U1 snRNP complex, the commitment complex; interacts with Msl5p/BBP splicing factor and Sub2p; similar to metazoan splicing factor U2AF65" [yeast-bovrabprots.fasta] [chemostatdb.fasta] Splicing factor MUD2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=MUD2 PE=1 SV=3 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] MUD2 SGDID:S000001557, Chr XI from 296193-294610, Genome Release 64-1-1, reverse complement, Verified ORF, "Protein involved in early pre-mRNA splicing; component of the pre-mRNA-U1 snRNP complex, the commitment complex; interacts with Msl5p/BBP splicing factor and Sub2p; similar to metazoan splicing factor U2AF65" [yeast-030211-contam.fasta] MUD2 SGDID:S000001557, Chr XI from 296193-294610, Genome Release 64-2-1, reverse complement, Verified ORF, "Protein involved in early pre-mRNA splicing; component of the pre-mRNA-U1 snRNP complex, the commitment complex; interacts with Msl5p/BBP splicing factor and Sub2p; similar to metazoan splicing factor U2AF65" [20141208_orf_trans.fasta] YKL074C SGDID:S000001557, Chr XI from 296193-294610, Genome Release 64-1-1, reverse complement, Verified ORF, "Protein involved in early pre-mRNA splicing; component of the pre-mRNA-U1 snRNP complex, the commitment complex; interacts with Msl5p/BBP splicing factor and Sub2p; similar to metazoan splicing factor U2AF65" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	527 amino acids
Gene Ontology:
Cellular Component:	commitment complex [IPI]
Biological Process:	RNA splicing [IEA] nuclear mRNA branch site recognition [IGI][IMP] mRNA processing [IEA]
Molecular Function:	mRNA binding [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MADEKRLEDL RSKIMESIGK SEKDVVPIEN KRFNTDNAVI DTHFKRQKSD GELPKAPKSR  60
   61 NVSHSNNRGP SSIITMSTNR TTYEQTRAGP HRQSYRDASG RSYNRENRYS SHNTGPQWNN 120
  121 NPYNRQRDER RGRNERFDRR GRNGNGNYDR FNYQRKNEGS KFNGDRDKRQ LQTNKYDMNY 180
  181 NSQNVMYPGS SFDSPAYYNM ASSKANSRLV ISGLSQSSDP SIVARLKDLL ENFISGLQKT 240
  241 ESNAEDFKIS NFYIGEGRPD HIIVEFSSQI CSTMVLACRS FFNAKLGTFD LKWRRPNDYV 300
  301 QQLDHLVDFC RGTVIALENL ENIGEGEDYR MKELFSSLNV TNGTAKPLFY KCSSNTNNTG 360
  361 KESEFTKCIL LSFEVVTQDI LDKLKPYKWF KPNDGKISQV TSWITFQSLP NLVTQSVRVE 420
  421 SRVLLLLNCL DPLDLKDETF ITEIKETLKY SIAGADTIKI CQPGVDYRLN FENLASGAGN 480
  481 IYIKFKTLEA AKHAMEELPG TQFNDRTVLC TYIDEDDFDM MEATQLS

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	(MIPS) Mewes HW, et al. (2004)		AAR2 BRR1 BRR2 CBC2 CDC40 CWC15 CWC2 CWC22 CWC23 DHR2 LSM4 MAK5 MUD2 PRP16 PRP18 PRP2 PRP22 PRP24 PRP28 PRP31 PRP38 PRP39 PRP4 PRP40 PRP43 PRP5 PRP6 PRP8 SLU7 SMD1 SMD3 SME1 SNP1 SPP2 SPP381 STO1 YHC1
View Details	Krogan NJ, et al. (2006)		MSL5 MUD2
View Details	Qiu et al. (2008)		AAR2 BRR1 BRR2 CBC2 CDC40 CEF1 CLF1 CUS1 CUS2 CWC2 CWC22 CWC23 DIB1 ECM2 HSH155 IST3 ISY1 LEA1 LSM2 LSM3 LSM4 LSM5 LSM6 LSM7 LSM8 LUC7 MAK5 MSL5 MUD1 MUD2 NAM8 NTC20 NTR2 PRP11 PRP16 PRP19 PRP2 PRP21 PRP22 PRP24 PRP28 PRP3 PRP31 PRP38 PRP39 PRP4 PRP40 PRP42 PRP43 PRP45 PRP46 PRP5 PRP6 PRP8 PRP9 RSE1 SLU7 SMB1 SMD1 SMD2 SMD3 SME1 SMX2 SMX3 SNP1 SNT309 SNU114 SNU23 SNU56 SNU66 SNU71 SPP381 SPP382 STO1 SUB2 SYF1 SYF2 YHC1

Mass Spectrometry Data

No mass spectrometry results found for this protein.

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	SPT21	MUD2	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	MUD2	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..392]	MSA	1.003	View MSA. No confident structure predictions are available.
2	View Details	[393..527]	PSI-BLAST	15.522879	U2AF35 (35 KDa subunit)
3	View Details	[416..527]	PSI-BLAST	27.221849	No description for 2pe8A was found.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2