Protein Overview: NUP120

General Information:
Name(s) found:	NUP120 / YKL057C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6322793\|ref\|NP_012866.1\| Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC), required for even distribution of NPCs around the nuclear envelope, involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPas [NCBI NR] gi\|549682\|sp\|P35729.1\|NU120_YEAST RecName: Full=Nucleoporin NUP120; AltName: Full=Nuclear pore protein NUP120 [NCBI NR] pir\|\|S37879 nuclear pore protein NUP120 - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|486153\|emb\|CAA81894.1\| NUP120 [Saccharomyces cerevisiae] [NCBI NR] Nucleoporin NUP120 OS=Saccharomyces cerevisiae GN=NUP120 PE=1 SV=1 [Swiss-Prot] (P35729) Nucleoporin NUP120 (Nuclear pore protein NUP120) [Swiss-Prot] NUP120 Nuclear pore protein [mips] Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC), required for even distribution of NPCs around the nuclear envelope, involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p [SGD] NUP120, Chr XI from 330498-333611, reverse complement [yeast_orfs2] Chr XI from 330498-333611, reverse complement [yeastorf3.fasta] Chr XI from 330497-333610, reverse complement [yeastorf4.fasta] Chr XI from 333610-330497, reverse complement [yeastorf5.fasta] NUP120 SGDID:S0001540, Chr XI from 333614-330501, reverse complement, Verified ORF [yeastflysequest.fasta] Nup120p [Saccharomyces cerevisiae]gi\|486153\|emb\|CAA81894.1\| NUP120 [Saccharomyces cerevisiae]gi\|539167\|pir\|\|S37879 nuclear pore protein NUP120 - yeast (Saccharomyces cerevisiae)gi\|549682\|sp\|P35729\|N120_YEAST Nucleoporin NUP120 (Nuclear pore protein NUP [nrdb_11152004_Chlamydomonas] NUP120 SGDID:S000001540, Chr XI from 333613-330500, reverse complement, Verified ORF, "Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC), required for even distribution of NPCs around the nuclear envelope, involved in establishment of a n [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] Nucleoporin NUP120 (Nuclear pore protein NUP120) [nr-271106-contam.fasta] NUP120 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC), required for even distribution of NPCs around the nuclear envelope, involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; Nup120p [Saccharomy [nr-271106-contam.fasta] NUP120 SGDID:S000001540, Chr XI from 333613-330500, reverse complement, Verified ORF, "Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC), required for even distribution of NPCs around the nuclear envelope, involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p" [yeast-bovrabprots.fasta] [chemostatdb.fasta] Nucleoporin NUP120 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=NUP120 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] NUP120 SGDID:S000001540, Chr XI from 333969-330856, Genome Release 64-1-1, reverse complement, Verified ORF, "Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); required for even distribution of NPCs around the nuclear envelope, involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; the Nup84 subcomplex has a role in transcription elongation" [yeast-030211-contam.fasta] NUP120 SGDID:S000001540, Chr XI from 333969-330856, Genome Release 64-2-1, reverse complement, Verified ORF, "Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP160" [20141208_orf_trans.fasta] YKL057C SGDID:S000001540, Chr XI from 333969-330856, Genome Release 64-1-1, reverse complement, Verified ORF, "Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); required for even distribution of NPCs around the nuclear envelope, involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; the Nup84 subcomplex has a role in transcription elongation" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	1037 amino acids
Gene Ontology:
Cellular Component:	nuclear pore [IDA]
Biological Process:	mRNA export from nucleus [IMP] rRNA export from nucleus [TAS] nuclear pore organization [IMP] snRNP protein import into nucleus [TAS] mRNA-binding (hnRNP) protein import into nucleus [TAS] snRNA export from nucleus [TAS] chromosome localization [IMP] tRNA export from nucleus [TAS] NLS-bearing substrate import into nucleus [TAS] DNA metabolic process [IGI] protein export from nucleus [TAS] ribosomal protein import into nucleus [TAS]
Molecular Function:	structural molecule activity [TAS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MACLSRIDAN LLQYYEKPEP NNTVDLYVSN NSNNNGLKEG DKSISTPVPQ PYGSEYSNCL  60
   61 LLSNSEYICY HFSSRSTLLT FYPLSDAYHG KTINIHLPNA SMNQRYTLTI QEVEQQLLVN 120
  121 VILKDGSFLT LQLPLSFLFS SANTLNGEWF HLQNPYDFTV RVPHFLFYVS PQFSVVFLED 180
  181 GGLLGLKKVD GVHYEPLLFN DNSYLKSLTR FFSRSSKSDY DSVISCKLFH ERYLIVLTQN 240
  241 CHLKIWDLTS FTLIQDYDMV SQSDSDPSHF RKVEAVGEYL SLYNNTLVTL LPLENGLFQM 300
  301 GTLLVDSSGI LTYTFQNNIP TNLSASAIWS IVDLVLTRPL ELNVEASYLN LIVLWKSGTA 360
  361 SKLQILNVND ESFKNYEWIE SVNKSLVDLQ SEHDLDIVTK TGDVERGFCN LKSRYGTQIF 420
  421 ERAQQILSEN KIIMAHNEDE EYLANLETIL RDVKTAFNEA SSITLYGDEI ILVNCFQPYN 480
  481 HSLYKLNTTV ENWFYNMHSE TDGSELFKYL RTLNGFASTL SNDVLRSISK KFLDIITGEL 540
  541 PDSMTTVEKF TDIFKNCLEN QFEITNLKIL FDELNSFDIP VVLNDLINNQ MKPGIFWKKD 600
  601 FISAIKFDGF TSIISLESLH QLLSIHYRIT LQVLLTFVLF DLDTEIFGQH ISTLLDLHYK 660
  661 QFLLLNLYRQ DKCLLAEVLL KDSSEFSFGV KFFNYGQLIA YIDSLNSNVY NASITENSFF 720
  721 MTFFRSYIIE NTSHKNIRFF LENVECPFYL RHNEVQEFMF AMTLFSCGNF DQSYEIFQLH 780
  781 DYPEAINDKL PTFLEDLKSE NYHGDSIWKD LLCTFTVPYR HSAFYYQLSL LFDRNNSQEF 840
  841 ALKCISKSAE YSLKEIQIEE LQDFKEKQHI HYLNLLIHFR MFEEVLDVLR LGHECLSDTV 900
  901 RTNFLQLLLQ EDIYSRDFFS TLLRLCNAHS DNGELYLRTV DIKIVDSILS QNLRSGDWEC 960
  961 FKKLYCFRML NKSERAAAEV LYQYILMQAD LDVIRKRKCY LMVINVLSSF DSAYDQWILN1020
 1021 GSKVVTLTDL RDELRGL

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		NUP120 NUP145 NUP84 NUP85 SEC13 SEH1
View Details	(MIPS) Mewes HW, et al. (2004)		NUP120 NUP145 NUP84 NUP85 SEC13 SEH1
View Details	Krogan NJ, et al. (2006)		MEX67 NUP120 NUP145 NUP84 NUP85 SEH1
View Details	Gavin AC, et al. (2006)		NUP120 NUP145 NUP84 NUP85 SEC13 SEH1
View Details	Gavin AC, et al. (2002)		CKA1 CKA2 CKB1 DBP2 MRPS28 NUP120 NUP145 NUP84 NUP85 RPP2B SEC13 SEC16 SEC23 SEC31 SEH1 STM1
View Details	Ho Y, et al. (2002)		NUP120 NUP84 PHO86
View Details	Qiu et al. (2008)		ASM4 KAP123 NIC96 NUP100 NUP116 NUP120 NUP133 NUP145 NUP157 NUP159 NUP170 NUP188 NUP57 NUP82 NUP84 NUP85 POM152 SEC13 SEH1 SUS1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	ZDS1	Sample 2- cpn2 from june 2005	McCusker D, et al (2007)
View Run	ZDS1	Sample 3 - cpn + atp, from june 2005	McCusker D, et al (2007)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	CPR6	NUP120	2	Unpublished Fields Lab Data
View Screen	CIC1	NUP120	1	Unpublished Fields Lab Data
View Screen	TRL1	NUP120	1	Unpublished Fields Lab Data
View Screen	HMG1	NUP120	1	Unpublished Fields Lab Data
View Screen	SPT21	NUP120	1	Unpublished Fields Lab Data
View Screen	MPC54	NUP120	1	Unpublished Fields Lab Data
View Screen	PHO85	NUP120	1	Unpublished Fields Lab Data
View Screen	YPR097W	NUP120	1	Unpublished Fields Lab Data
View Screen	AIM13	NUP120	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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No microscopy data found in the PDR for this protein.

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..479]	MSA	1.002	View MSA. No confident structure predictions are available.
2	View Details	[480..1037]	MSA	1.002001	View MSA. No confident structure predictions are available.
3	View Details	[590..745]	deduced	N/A	No confident structure predictions are available.
4	View Details	[746..1037]	FFAS03	0.975	Vesicular transport protein sec17

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
nucleocytoplasmic transporter activity	6.69510224853745	bayes_pls_golite062009
transporter activity	2.44173429092591	bayes_pls_golite062009
binding	1.85742618935461	bayes_pls_golite062009
structural molecule activity	1.08319592328349	bayes_pls_golite062009
protein binding	0.749555781220334	bayes_pls_golite062009

No functions predicted.

Term	Confidence	Notes
binding	2.50062202757362	bayes_pls_golite062009
protein binding	1.72061787550863	bayes_pls_golite062009
signal sequence binding	1.06643404565266	bayes_pls_golite062009

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.97

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2