Protein Overview: DID4

General Information:
Name(s) found:	DID4 / YKL002W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|9755336\|ref\|NP_012924.2\| Class E Vps protein of the ESCRT-III complex, required for sorting of integral membrane proteins into lumenal vesicles of multivesicular bodies, and for delivery of newly synthesized vacuolar enzymes to the vacuole, involved in [NCBI NR] gi\|116241331\|sp\|P36108.2\|DID4_YEAST RecName: Full=DOA4-independent degradation protein 4; AltName: Full=ESCRT-III complex subunit VPS2; AltName: Full=Vacuolar protein-sorting-associated protein 2; AltName: Full=Vacuolar protein-targeting protein 14 [NCBI NR] DOA4-independent degradation protein 4 OS=Saccharomyces cerevisiae GN=DID4 PE=1 SV=2 [Swiss-Prot] DID4 Class E vacuolar-protein sorting and endocytosis factor [mips] Class E Vps protein of the ESCRT-III complex, required for sorting of integral membrane proteins into lumenal vesicles of multivesicular bodies, and for delivery of newly synthesized vacuolar enzymes to the vacuole, involved in endocytosis [SGD] DID4, Chr XI from 437416-437475,437544-438182 [yeast_orfs2] Chr XI from 437416-437475,437544-438182 [yeastorf3.fasta] DID4 SGDID:S0001485, Chr XI from 437421-437480,437549-438187, Verified ORF [yeastflysequest.fasta] Class E Vps protein of the ESCRT-III complex, required for sorting of integral membrane proteins into lumenal vesicles of multivesicular bodies, and for delivery of newly synthesized vacuolar enzymes to the vacuole, involved in endocytosis [Saccharomyces [nrdb_11152004_Chlamydomonas] DID4 SGDID:S000001485, Chr XI from 437421-437480,437549-438187, Verified ORF, "Class E Vps protein of the ESCRT-III complex, required for sorting of integral membrane proteins into lumenal vesicles of multivesicular bodies, and for delivery of newly synth [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] Class E Vps protein of the ESCRT-III complex, required for sorting of integral membrane proteins into lumenal vesicles of multivesicular bodies, and for delivery of newly synthesized vacuolar enzymes to the vacuole, involved in endocytosis; Did4p [Sacchar [nr-271106-contam.fasta] DOA4-independent degradation protein 4 (Vacuolar protein sorting-associated protein 2) (Vacuolar protein-targeting protein 14) [nr-271106-contam.fasta] DID4 SGDID:S000001485, Chr XI from 437421-437480,437549-438187, Verified ORF, "Class E Vps protein of the ESCRT-III complex, required for sorting of integral membrane proteins into lumenal vesicles of multivesicular bodies, and for delivery of newly synthesized vacuolar enzymes to the vacuole, involved in endocytosis" [yeast-bovrabprots.fasta] [chemostatdb.fasta] DOA4-independent degradation protein 4 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=DID4 PE=1 SV=2 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] DID4 SGDID:S000001485, Chr XI from 437778-437837,437906-438544, Genome Release 64-1-1, Verified ORF, "Class E Vps protein of the ESCRT-III complex, required for sorting of integral membrane proteins into lumenal vesicles of multivesicular bodies, and for delivery of newly synthesized vacuolar enzymes to the vacuole, involved in endocytosis" [yeast-030211-contam.fasta] DID4 SGDID:S000001485, Chr XI from 437778-437837,437906-438544, Genome Release 64-2-1, Verified ORF, "Class E Vps protein of the ESCRT-III complex; required for sorting of integral membrane proteins into lumenal vesicles of multivesicular bodies, and for delivery of newly synthesized vacuolar enzymes to the vacuole, involved in endocytosis" [20141208_orf_trans.fasta] YKL002W SGDID:S000001485, Chr XI from 437778-437837,437906-438544, Genome Release 64-1-1, Verified ORF, "Class E Vps protein of the ESCRT-III complex, required for sorting of integral membrane proteins into lumenal vesicles of multivesicular bodies, and for delivery of newly synthesized vacuolar enzymes to the vacuole, involved in endocytosis" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	232 amino acids
Gene Ontology:
Cellular Component:	ESCRT III complex [IDA] cytoplasm [IDA]
Biological Process:	protein retention in Golgi apparatus [IMP] ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway [IC] late endosome to vacuole transport [IPI]
Molecular Function:	protein binding [TAS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSLFEWVFGK NVTPQERLKK NQRALERTQR ELEREKRKLE LQDKKLVSEI KKSAKNGQVA  60
   61 AAKVQAKDLV RTRNYIQKFD NMKAQLQAIS LRIQAVRSSD QMTRSMSEAT GLLAGMNRTM 120
  121 NLPQLQRISM EFEKQSDLMG QRQEFMDEAI DNVMGDEVDE DEEADEIVNK VLDEIGVDLN 180
  181 SQLQSTPQNL VSNAPIAETA MGIPEPIGAG SEFHGNPDDD LQARLNTLKK QT

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Qiu et al. (2008)		DID4 MVB12 SNF7 SRN2 VPS20 VPS24 VPS27 VPS28 VPS35 VPS36

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	VAM3	Sample: HX12 - trans-SNARE complex formation and fusion are inhibited (control).	Hao Xu, et al. (2010)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	PHS1	DID4	1	Unpublished Fields Lab Data
View Screen	RPL33A	DID4	1	Unpublished Fields Lab Data
View Screen	DAS1	DID4	1	Unpublished Fields Lab Data
View Screen	SAS3	DID4	1	Unpublished Fields Lab Data
View Screen	NUC1	DID4	1	Unpublished Fields Lab Data
View Screen	ILM1	DID4	1	Lockshon D, et al. (2006)
View Screen	RAD26	DID4	1	Unpublished Fields Lab Data
View Screen	IML1	DID4	1	Unpublished Fields Lab Data
View Screen	CDC16	DID4	1	Unpublished Fields Lab Data
View Screen	PRR1	DID4	1	Unpublished Fields Lab Data
View Screen	RFS1	DID4	1	Unpublished Fields Lab Data
View Screen	LOS1	DID4	1	Unpublished Fields Lab Data
View Screen	GBP2	DID4	1	Unpublished Fields Lab Data
View Screen	LOS1	DID4	1	Unpublished Fields Lab Data
View Screen	CDC53	DID4	1	Unpublished Fields Lab Data
View Screen	TEM1	DID4	1	Unpublished Fields Lab Data
View Screen	RPN5	DID4	1	Unpublished Fields Lab Data
View Screen	SPT21	DID4	1	Unpublished Fields Lab Data
View Screen	YDR049W	DID4	1	Unpublished Fields Lab Data
View Screen	MPD2	DID4	1	Unpublished Fields Lab Data
View Screen	GLC7	DID4	1	Unpublished Fields Lab Data
View Screen	VPS17	DID4	1	Unpublished Fields Lab Data
View Screen	YGL108C	DID4	1	Unpublished Fields Lab Data
View Screen	RPB2	DID4	1	Unpublished Fields Lab Data
View Screen	KSS1	DID4	1	Unpublished Fields Lab Data
View Screen	RAD1	DID4	1	Unpublished Fields Lab Data
View Screen	TRL1	DID4	1	Unpublished Fields Lab Data
View Screen	PHO85	DID4	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	DID4 and SNF7	Huh WK, et al. (2003)
View Data	DID4	Huh WK, et al. (2003)

Protein Structure Data

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Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..232]	Pfam	35.040959	No description for PF03357.7 was found. No confident structure predictions are available.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2