Protein Overview: FKH1

General Information:
Name(s) found:	FKH1 / YIL131C [SGD]
Description(s) found: Found 31 descriptions. SHOW ALL	Forkhead family transcription factor with a minor role in the expression of G2/M phase genes; negatively regulates transcriptional elongation; positive role in chromatin silencing at HML and HMR; regulates donor preference during switching [SGD]
Organism:	Saccharomyces cerevisiae
Length:	484 amino acids
Gene Ontology:
Cellular Component:	nucleus [IDA]
Biological Process:	pseudohyphal growth [IGI ] negative regulation of cell cycle [IMP] positive regulation of cell cycle [IGI ] regulation of transcription involved in G2/M-phase of mitotic cell cycle [IGI ] termination of RNA polymerase II transcription [IGI][IMP] donor selection [IGI ][IMP ][IPI] chromatin silencing at silent mating-type cassette [IGI][IMP] RNA elongation from RNA polymerase II promoter [IMP][IPI]
Molecular Function:	DNA binding [IDA] specific RNA polymerase II transcription factor activity [IGI ][ISS ] centromeric DNA binding [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSVTSREQKF SGKYSSYTAQ DRQGLVNAVT CVLSSSSDPV AVSSDYSNSL SIAREVNAYA  60
   61 KIAGCDWTYY VQKLEVTIGR NTDSLNLNAV PGTVVKKNID IDLGPAKIVS RKHAAIRFNL 120
  121 ESGSWELQIF GRNGAKVNFR RIPTGPDSPP TVLQSGCIID IGGVQMIFIL PEQETIISDY 180
  181 CLNHLMPKLL STYGTNGNNN PLLRNIIEGS TYLREQRLQE EARLQQLDHL HTPLSSSSDV 240
  241 NPIGDPHGDT IMMEEDEEDE NYTRGGIRPN TYTSSSNNAV TNGNVPHIEN PSDLSLDENR 300
  301 YIKPPQSYAS MITQAILSTP EGSISLADIY KFISDNYAFY RFSQMAWQNS VRHNLSLNKA 360
  361 FEKVPKRAGQ QGKGMNWKIS DEVRRDFLNK WNAGKLSKIR RGASVTRQLQ LHMSKFGEIP 420
  421 APESSSIDPR GIKAQKVKKS LQATSSILGE SAPQLQRTQL TGQISTTTSM DVTTNANVNN 480
  481 SSLS

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Ho Y, et al. (2002)		CDC28 CEG1 CKA1 CKA2 CKB1 CKB2 FKH1 FYV8 GCD2 GCD7 GCN3 HHF1, HHF2 HTB1 MBP1 MGM101 MPH1 NET1 NOP1 RRP1 SEC2 SIN3 SUI2 SUI3 UBP12 URE2 YGR017W YMR144W
View Details	Qiu et al. (2008)		FKH1 FKH2

Mass Spectrometry Data

No mass spectrometry results found for this protein.

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	FMP48	FKH1	1	Unpublished Fields Lab Data
View Screen	UBC7	FKH1	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	FKH1	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..74]	deduced	N/A	No confident structure predictions are available.
2	View Details	[75..189]	Pfam	20.958607	FHA domain No confident structure predictions are available.
3	View Details	[190..294]	MSA	3.207997	View MSA. No confident structure predictions are available.
4	View Details	[295..414]	PSI-BLAST	229.529817	Adipocyte-transcription factor FREAC-11 (s12, fkh-14)
5	View Details	[415..484]	deduced	N/A	No confident structure predictions are available.

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
binding	3.49130250684713	bayes_pls_golite062009
transcription regulator activity	3.38780975952028	bayes_pls_golite062009
protein binding	3.25414571587883	bayes_pls_golite062009
DNA binding	3.17754626214699	bayes_pls_golite062009
nucleic acid binding	3.1171915055278	bayes_pls_golite062009
transcription activator activity	2.78535001965943	bayes_pls_golite062009
sequence-specific DNA binding	2.68762321363292	bayes_pls_golite062009
RNA polymerase II transcription factor activity	2.41431149922235	bayes_pls_golite062009
transcription factor activity	2.00503872581265	bayes_pls_golite062009
transcription repressor activity	1.70605033973639	bayes_pls_golite062009
chromatin binding	1.24734533820287	bayes_pls_golite062009
transforming growth factor beta receptor, cytoplasmic mediator activity	1.22691767140341	bayes_pls_golite062009
promoter binding	1.19827477319554	bayes_pls_golite062009
transcription factor binding	0.840886266060694	bayes_pls_golite062009
transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity	0.829235284155381	bayes_pls_golite062009
structure-specific DNA binding	0.799061296723533	bayes_pls_golite062009
double-stranded DNA binding	0.609339192036706	bayes_pls_golite062009
transmembrane receptor protein serine/threonine kinase signaling protein activity	0.557735630718468	bayes_pls_golite062009
SMAD binding	0.421497570548243	bayes_pls_golite062009
receptor signaling protein activity	0.196567610974905	bayes_pls_golite062009
protein dimerization activity	0.195556172286344	bayes_pls_golite062009
R-SMAD binding	0.18488905369604	bayes_pls_golite062009

No functions predicted.

Term	Confidence	Notes
transcription regulator activity	5.55329976718261	bayes_pls_golite062009
DNA binding	5.08959163553491	bayes_pls_golite062009
nucleic acid binding	4.99681957904233	bayes_pls_golite062009
transcription factor activity	4.33152142980289	bayes_pls_golite062009
transcription repressor activity	3.85678231380989	bayes_pls_golite062009
transcription activator activity	3.70589483230587	bayes_pls_golite062009
sequence-specific DNA binding	3.58179858678904	bayes_pls_golite062009
binding	3.52874320222492	bayes_pls_golite062009
RNA polymerase II transcription factor activity	2.60738445921725	bayes_pls_golite062009
protein binding	1.80415286835431	bayes_pls_golite062009
specific transcriptional repressor activity	0.96257232341735	bayes_pls_golite062009
transcription factor binding	0.85714789329232	bayes_pls_golite062009
transcription elongation regulator activity	0.801431089034975	bayes_pls_golite062009
specific RNA polymerase II transcription factor activity	0.66743181561551	bayes_pls_golite062009
transcription termination factor activity	0.471608452842719	bayes_pls_golite062009
structure-specific DNA binding	0.379762425417023	bayes_pls_golite062009
transcription corepressor activity	0.322180400748724	bayes_pls_golite062009
RNA polymerase II transcription factor activity, enhancer binding	0.23177057841536	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.96

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2