A winged helix protein from yeast Saccharomyces cerevisiae recognizes centromere sequences

Arch Biochem Biophys. 2000 Mar 1;375(1):78-82. doi: 10.1006/abbi.1999.1638.

Abstract

The winged helix-turn-helix motif was initially identified in the mammalian hepatocyte-enriched transcription factor HNF-3 and the Drosophila forkhead homeotic protein. Proteins containing the winged helix motif have been shown to play important roles in tissue-specific developmental regulation. In this report, by using a genomic binding site selection method, we demonstrate that the winged helix protein YFKH-1 from the yeast Saccharomyces cerevisiae recognizes conserved sequence in yeast centromeres. Thus, our data suggest that the winged helix proteins of the yeast may be involved in centromeric functions of the yeast.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Binding Sites / genetics
  • Centromere / metabolism*
  • Conserved Sequence / genetics
  • DNA, Fungal / metabolism
  • DNA-Binding Proteins / genetics*
  • DNA-Binding Proteins / metabolism
  • Fungal Proteins / genetics*
  • Fungal Proteins / metabolism
  • Molecular Sequence Data
  • Plasmids / genetics
  • Plasmids / isolation & purification
  • Protein Structure, Tertiary / genetics
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / metabolism
  • Saccharomyces cerevisiae Proteins*
  • Sequence Homology, Amino Acid

Substances

  • DNA, Fungal
  • DNA-Binding Proteins
  • Fungal Proteins
  • Saccharomyces cerevisiae Proteins
  • YFKH-1 protein, S cerevisiae