Protein Overview: SPT3

General Information:
Name(s) found:	SPT3 / YDR392W [SGD]
Description(s) found: Found 43 descriptions. SHOW ALL	Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters [SGD]
Organism:	Saccharomyces cerevisiae
Length:	337 amino acids
Gene Ontology:
Cellular Component:	SLIK (SAGA-like) complex [IPI] SAGA complex [IDA]
Biological Process:	chromatin modification [TAS] ascospore formation [TAS] conjugation with cellular fusion [TAS] pseudohyphal growth [IDA] histone acetylation [TAS] invasive growth in response to glucose limitation [IDA]
Molecular Function:	transcription cofactor activity [IDA][IMP]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MMDKHKYRVE IQQMMFVSGE INDPPVETTS LIEDIVRGQV IEILLQSNKT AHLRGSRSIL  60
   61 PEDVIFLIRH DKAKVNRLRT YLSWKDLRKN AKDQDASAGV ASGTGNPGAG GEDDLKKAGG 120
  121 GEKDEKDGGN MMKVKKSQIK LPWELQFMFN EHPLENNDDN DDMDEDEREA NIVTLKRLKM 180
  181 ADDRTRNMTK EEYVHWSDCR QASFTFRKNK RFKDWSGISQ LTEGKPHDDV IDILGFLTFE 240
  241 IVCSLTETAL KIKQREQVLQ TQKDKSQQSS QDNTNFEFAS STLHRKKRLF DGPENVINPL 300
  301 KPRHIEEAWR VLQTIDMRHR ALTNFKGGRL SSKPIIM

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		ADA2 GCN5 HFI1 NGG1 SGF29 SGF73 SPT20 SPT3 SPT7 SPT8 TAF10 TAF11 TAF12 TAF5 TAF6 TAF9 TRA1 UBP8
View Details	(MIPS) Mewes HW, et al. (2004)		ADA2 GCN5 HFI1 NGG1 SGF29 SGF73 SPT20 SPT3 SPT7 SPT8 TAF10 TAF12 TAF5 TAF6 TAF9 TRA1
View Details	Krogan NJ, et al. (2006)		ADA2 AHC1 AHC2 FMP40 GCN5 HFI1 MSS4 NGG1 PDR15 RAD59 REV3 SGF11 SGF29 SGF73 SNL1 SPT20 SPT3 SPT7 SPT8 TAF1 TAF10 TAF11 TAF12 TAF13 TAF3 TAF4 TAF5 TAF6 TAF7 TAF9 UBP8 YMR007W
View Details	Gavin AC, et al. (2006)		ADA2 HFI1 NGG1 SGF29 SGF73 SPT20 SPT3 SPT7 SPT8
View Details	Qiu et al. (2008)		ADA2 GCN5 HFI1 MED2 MED6 NGG1 SGF29 SGF73 SPT20 SPT3 SPT7 SPT8 SRB6 SWC4 TAF10 TAF11 TAF12 TAF13 TAF14 TAF3 TAF5 TAF6 TAF9 TRA1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	CEP3	sample cbf3 from feb 2005	Sandall S, et all (2006)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	SWI5	SPT3	1	Unpublished Fields Lab Data
View Screen	YPT31	SPT3	1	Unpublished Fields Lab Data
View Screen	RPN11	SPT3	1	Unpublished Fields Lab Data
View Screen	VAM7	SPT3	1	Unpublished Fields Lab Data
View Screen	GYP6	SPT3	1	Unpublished Fields Lab Data
View Screen	PUF3	SPT3	1	Unpublished Fields Lab Data
View Screen	RRB1	SPT3	1	Unpublished Fields Lab Data
View Screen	CUS1	SPT3	1	Unpublished Fields Lab Data
View Screen	CLB2	SPT3	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	SPT3	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..50]	PSI-BLAST	13.39794	TAF(II)18
2	View Details	[51..109]	Pfam	6.327902	Transcription initiation factor IID, 18kD subunit No confident structure predictions are available.
3	View Details	[110..337]	deduced	N/A	No confident structure predictions are available.

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
general RNA polymerase II transcription factor activity	6.26683920136262	bayes_pls_golite062009
lysine N-acetyltransferase activity	5.93249134612505	bayes_pls_golite062009
histone acetyltransferase activity	5.93249134612505	bayes_pls_golite062009
N-acetyltransferase activity	4.49084162254966	bayes_pls_golite062009
DNA binding	4.24660531286828	bayes_pls_golite062009
N-acyltransferase activity	4.2172829451557	bayes_pls_golite062009
nucleic acid binding	4.14026521609645	bayes_pls_golite062009
transcription regulator activity	3.91807454227165	bayes_pls_golite062009
acetyltransferase activity	3.91533222675137	bayes_pls_golite062009
acyltransferase activity	3.38996237245502	bayes_pls_golite062009
transferase activity, transferring acyl groups other than amino-acyl groups	3.35419529667223	bayes_pls_golite062009
transferase activity, transferring acyl groups	3.18312128320637	bayes_pls_golite062009
binding	3.14832737946099	bayes_pls_golite062009
RNA polymerase II transcription factor activity	3.02428691626433	bayes_pls_golite062009
transcription factor activity	2.39322722597434	bayes_pls_golite062009
transcription activator activity	1.12739348005764	bayes_pls_golite062009
nucleotidyltransferase activity	1.03060091158028	bayes_pls_golite062009
protein binding	1.01897642648285	bayes_pls_golite062009
transcription factor binding	1.01773884290535	bayes_pls_golite062009
transcription coactivator activity	0.976797801726735	bayes_pls_golite062009
transcription cofactor activity	0.840442755533537	bayes_pls_golite062009
transcription initiation factor activity	0.718301463460921	bayes_pls_golite062009
transcription repressor activity	0.621482805601238	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	0.450418553112188	bayes_pls_golite062009
histone binding	0.184823910505751	bayes_pls_golite062009
transferase activity	0.137803992484684	bayes_pls_golite062009
sequence-specific DNA binding	0.0585868733603467	bayes_pls_golite062009

Term	Confidence	Notes
general RNA polymerase II transcription factor activity	6.26683920136262	bayes_pls_golite062009
lysine N-acetyltransferase activity	5.93249134612505	bayes_pls_golite062009
histone acetyltransferase activity	5.93249134612505	bayes_pls_golite062009
N-acetyltransferase activity	4.49084162254966	bayes_pls_golite062009
DNA binding	4.24660531286828	bayes_pls_golite062009
N-acyltransferase activity	4.2172829451557	bayes_pls_golite062009
nucleic acid binding	4.14026521609645	bayes_pls_golite062009
transcription regulator activity	3.91807454227165	bayes_pls_golite062009
acetyltransferase activity	3.91533222675137	bayes_pls_golite062009
acyltransferase activity	3.38996237245502	bayes_pls_golite062009
transferase activity, transferring acyl groups other than amino-acyl groups	3.35419529667223	bayes_pls_golite062009
transferase activity, transferring acyl groups	3.18312128320637	bayes_pls_golite062009
binding	3.14832737946099	bayes_pls_golite062009
RNA polymerase II transcription factor activity	3.02428691626433	bayes_pls_golite062009
transcription factor activity	2.39322722597434	bayes_pls_golite062009
transcription activator activity	1.12739348005764	bayes_pls_golite062009
nucleotidyltransferase activity	1.03060091158028	bayes_pls_golite062009
protein binding	1.01897642648285	bayes_pls_golite062009
transcription factor binding	1.01773884290535	bayes_pls_golite062009
transcription coactivator activity	0.976797801726735	bayes_pls_golite062009
transcription cofactor activity	0.840442755533537	bayes_pls_golite062009
transcription initiation factor activity	0.718301463460921	bayes_pls_golite062009
transcription repressor activity	0.621482805601238	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	0.450418553112188	bayes_pls_golite062009
histone binding	0.184823910505751	bayes_pls_golite062009
transferase activity	0.137803992484684	bayes_pls_golite062009
sequence-specific DNA binding	0.0585868733603467	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2