YRC Public Data Repository - Protein Structure Prediction

Protein:	SPT3
Organism:	Saccharomyces cerevisiae
Length:	337 amino acids
Reference:	Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for SPT3.

Description	E-value	Query Range	Subject Range
SPT3 - Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to act... gi\|190404670 - gi\|190404670\|gb\|EDV07937.1\| protein SPT3 [Saccharomyces cerevisiae RM11-1a] SPT3_YEAST - Protein SPT3 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=SPT3 PE=1 SV=1	0.0	[1..337]	[1..337]
gi\|4929173 - gi\|4929173\|gb\|AAD33888.1\|AF142757_1 transcription factor Spt3 [Candida albicans] gi\|46441197, gi\|... - gi\|68473155\|ref\|XP_719383.1\| hypothetical protein CaO19.7622 [Candida albicans SC5314], gi\|46441197\|... gi\|238880407 - gi\|238880407\|gb\|EEQ44045.1\| protein SPT3 [Candida albicans WO-1]	0.0	[3..337]	[5..328]
SPT3_KLULA - Protein SPT3 OS=Kluyveromyces lactis GN=SPT3 PE=4 SV=1 SPT3_KLULA - Protein SPT3 OS=Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1...	0.0	[1..337]	[1..328]
gi\|2253305 - gi\|2253305\|gb\|AAC49996.1\| Spt3 [Clavispora opuntiae]	0.0	[14..337]	[1..306]
SPT3_SCHPO - SAGA complex subunit spt3 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=spt3 PE=1 SV=2 spt3 - histone acetyltransferase complex subunit Spt3	0.0	[3..337]	[6..307]
gi\|2253303 - gi\|2253303\|gb\|AAC49995.1\| Spt3 [Schizosaccharomyces pombe]	0.0	[3..337]	[6..307]
gi\|3328173, gi\|3... - gi\|56203973\|emb\|CAI19927.1\| suppressor of Ty 3 homolog (S. cerevisiae) [Homo sapiens], gi\|55957996\|e...	2.0E-86	[7..312]	[26..293]
Spt3-PA - The gene Spt3 is referred to in FlyBase by the symbol Dmel\Spt3 (CG3169, FBgn0037981). It is a prote...	2.0E-71	[9..313]	[104..359]

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Predicted Domain #1

Region A:
Residues: [1-50]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MMDKHKYRVE IQQMMFVSGE INDPPVETTS LIEDIVRGQV IEILLQSNKT

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	13.39794
Match:	1bh8A_
Description:	TAF(II)18
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
general RNA polymerase II transcription factor activity	6.26683920136262	bayes_pls_golite062009
lysine N-acetyltransferase activity	5.93249134612505	bayes_pls_golite062009
histone acetyltransferase activity	5.93249134612505	bayes_pls_golite062009
N-acetyltransferase activity	4.49084162254966	bayes_pls_golite062009
DNA binding	4.24660531286828	bayes_pls_golite062009
N-acyltransferase activity	4.2172829451557	bayes_pls_golite062009
nucleic acid binding	4.14026521609645	bayes_pls_golite062009
transcription regulator activity	3.91807454227165	bayes_pls_golite062009
acetyltransferase activity	3.91533222675137	bayes_pls_golite062009
acyltransferase activity	3.38996237245502	bayes_pls_golite062009
transferase activity, transferring acyl groups other than amino-acyl groups	3.35419529667223	bayes_pls_golite062009
transferase activity, transferring acyl groups	3.18312128320637	bayes_pls_golite062009
binding	3.14832737946099	bayes_pls_golite062009
RNA polymerase II transcription factor activity	3.02428691626433	bayes_pls_golite062009
transcription factor activity	2.39322722597434	bayes_pls_golite062009
transcription activator activity	1.12739348005764	bayes_pls_golite062009
nucleotidyltransferase activity	1.03060091158028	bayes_pls_golite062009
protein binding	1.01897642648285	bayes_pls_golite062009
transcription factor binding	1.01773884290535	bayes_pls_golite062009
transcription coactivator activity	0.976797801726735	bayes_pls_golite062009
transcription cofactor activity	0.840442755533537	bayes_pls_golite062009
transcription initiation factor activity	0.718301463460921	bayes_pls_golite062009
transcription repressor activity	0.621482805601238	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	0.450418553112188	bayes_pls_golite062009
histone binding	0.184823910505751	bayes_pls_golite062009
transferase activity	0.137803992484684	bayes_pls_golite062009
sequence-specific DNA binding	0.0585868733603467	bayes_pls_golite062009

Predicted Domain #2

Region A:
Residues: [51-109]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 AHLRGSRSIL PEDVIFLIRH DKAKVNRLRT YLSWKDLRKN AKDQDASAGV ASGTGNPGA

[Run NCBI BLAST on this sequence.]

Detection Method:	Pfam
Confidence:	6.327902
Match:	PF02269
Description:	Transcription initiation factor IID, 18kD subunit

Shown below is our most confident prediction for this domain.
Click here to view all matches.

Found no confident structure predictions for this domain.

Predicted functions:

Term	Confidence	Notes
general RNA polymerase II transcription factor activity	6.26683920136262	bayes_pls_golite062009
lysine N-acetyltransferase activity	5.93249134612505	bayes_pls_golite062009
histone acetyltransferase activity	5.93249134612505	bayes_pls_golite062009
N-acetyltransferase activity	4.49084162254966	bayes_pls_golite062009
DNA binding	4.24660531286828	bayes_pls_golite062009
N-acyltransferase activity	4.2172829451557	bayes_pls_golite062009
nucleic acid binding	4.14026521609645	bayes_pls_golite062009
transcription regulator activity	3.91807454227165	bayes_pls_golite062009
acetyltransferase activity	3.91533222675137	bayes_pls_golite062009
acyltransferase activity	3.38996237245502	bayes_pls_golite062009
transferase activity, transferring acyl groups other than amino-acyl groups	3.35419529667223	bayes_pls_golite062009
transferase activity, transferring acyl groups	3.18312128320637	bayes_pls_golite062009
binding	3.14832737946099	bayes_pls_golite062009
RNA polymerase II transcription factor activity	3.02428691626433	bayes_pls_golite062009
transcription factor activity	2.39322722597434	bayes_pls_golite062009
transcription activator activity	1.12739348005764	bayes_pls_golite062009
nucleotidyltransferase activity	1.03060091158028	bayes_pls_golite062009
protein binding	1.01897642648285	bayes_pls_golite062009
transcription factor binding	1.01773884290535	bayes_pls_golite062009
transcription coactivator activity	0.976797801726735	bayes_pls_golite062009
transcription cofactor activity	0.840442755533537	bayes_pls_golite062009
transcription initiation factor activity	0.718301463460921	bayes_pls_golite062009
transcription repressor activity	0.621482805601238	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	0.450418553112188	bayes_pls_golite062009
histone binding	0.184823910505751	bayes_pls_golite062009
transferase activity	0.137803992484684	bayes_pls_golite062009
sequence-specific DNA binding	0.0585868733603467	bayes_pls_golite062009

Predicted Domain #3

Region A:
Residues: [110-337]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 GGEDDLKKAG GGEKDEKDGG NMMKVKKSQI KLPWELQFMF NEHPLENNDD NDDMDEDERE  60
   61 ANIVTLKRLK MADDRTRNMT KEEYVHWSDC RQASFTFRKN KRFKDWSGIS QLTEGKPHDD 120
  121 VIDILGFLTF EIVCSLTETA LKIKQREQVL QTQKDKSQQS SQDNTNFEFA SSTLHRKKRL 180
  181 FDGPENVINP LKPRHIEEAW RVLQTIDMRH RALTNFKGGR LSSKPIIM

[Run NCBI BLAST on this sequence.]

Detection Method:

deduced

Shown below is our most confident de novo (Rosetta) prediction for this domain.
Click here to view all matches.

Found no confident structure predictions for this domain.

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions:

Predicted functions: