Bait Protein: | DAD1 |
Organism: | Saccharomyces cerevisiae |
Publication: | Shimogawa M. M. et al. (2006) Mps1 phosphorylation of Dam1 couples kinetochores to microtubule plus ends at metaphase. Curr Biol. 2006 Aug 8;16(15):1489-501. |
Comments: | Dam1-765 complex purified from yeast with Dad1-TAP |
Raw Data: |
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FILTERED Run Results:
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Hit Protein | Protein Desc | Sequence Count | Spectrum Count | Sequence Coverage | NSAF | Mol. Wt. | |
[ |
ACC1 | Acetyl-CoA carboxylase, biotin containing enzyme that catalyzes the carboxylation of acetyl-CoA to f... | 4 | 4 | 3.1% | 2.48E-4 | 250351 |
[ |
ACT1 | Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functio... | 9 | 17 | 39.7% | 0.00628 | 41690 |
[ |
ADE5,7 | Bifunctional enzyme of the 'de novo' purine nucleotide biosynthetic pathway, contains aminoimidazole... | 5 | 7 | 13.1% | 0.00121 | 86068 |
[ |
ADH1 | Alcohol dehydrogenase, fermentative isozyme active as homo- or heterotetramers; required for the red... | 13 | 25 | 38.2% | 0.00994 | 36849 |
[ |
ALA1 | Cytoplasmic and mitochondrial alanyl-tRNA synthetase, required for protein synthesis; point mutation... | 3 | 3 | 5.3% | 4.33E-4 | 107277 |
[ |
APA1 | Diadenosine 5',5''-P1,P4-tetraphosphate phosphorylase I (AP4A phosphorylase), involved in catabolism... | 2 | 3 | 19.3% | 0.00129 | 36493 |
[ |
ARF1 | ADP-ribosylation factor, GTPase of the Ras superfamily involved in regulation of coated vesicle form... | 3 | 3 | 17.1% | 0.00229 | 20529 |
[ |
ASC1 | G-protein beta subunit and guanine nucleotide dissociation inhibitor for Gpa2p; ortholog of RACK1 th... | 14 | 24 | 64.3% | 0.0104 | 34805 |
[ |
ASK1 | Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced... | 25 | 68 | 84.6% | 0.0322 | 32056 |
[ |
ATP1 | Alpha subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily ... | 3 | 5 | 9.7% | 0.00127 | 58618 |
[ |
ATP2 | Beta subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily c... | 5 | 6 | 19.8% | 0.00163 | 54794 |
[ |
ATP3 | Gamma subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily ... | 5 | 5 | 23.5% | 0.00223 | 34351 |
[ |
CCT4 | Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of... | 4 | 5 | 17.2% | 0.00131 | 57604 |
[ |
CCT8 | Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of... | 2 | 2 | 4.4% | 4.87E-4 | 61662 |
[ |
CDC19 | Pyruvate kinase, functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvat... | 7 | 11 | 25.0% | 0.00305 | 54545 |
[ |
CYS4 | Cystathionine beta-synthase, catalyzes synthesis of cystathionine from serine and homocysteine, the ... | 2 | 2 | 7.7% | 5.46E-4 | 56022 |
[ |
DAD1 | Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced... | 9 | 19 | 97.9% | 0.028 | 10516 |
[ |
DAD3 | Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced... | 10 | 20 | 77.7% | 0.0295 | 10848 |
[ |
DAD4 | Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced... | 4 | 18 | 36.1% | 0.0346 | 8155 |
[ |
DAM1 | Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced... | 23 | 52 | 54.5% | 0.021 | 38422 |
[ |
DED1 | ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all ye... | 4 | 5 | 14.2% | 0.00115 | 65553 |
[ |
DPM1 | Dolichol phosphate mannose (Dol-P-Man) synthase of the ER membrane, catalyzes the formation of Dol-P... | 2 | 2 | 12.0% | 0.00104 | 30362 |
[ |
DUO1 | Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced... | 30 | 74 | 77.3% | 0.0415 | 27473 |
[ |
DUR1,2 | Urea amidolyase, contains both urea carboxylase and allophanate hydrolase activities, degrades urea ... | 2 | 2 | 2.8% | 1.51E-4 | 201830 |
[ |
EFT2, EFT1 | Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein s... | 8 | 11 | 16.3% | 0.00181 | 93289 |
[ |
ENO2 | Enolase II, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosp... | 2 | 5 | 11.9% | 0.00158 | 46914 |
[ |
ERG13 | 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase, catalyzes the formation of HMG-CoA from acetyl-Co... | 8 | 11 | 26.9% | 0.0031 | 55014 |
[ |
FAA1 | Long chain fatty acyl-CoA synthetase, activates imported fatty acids with a preference for C12:0-C16... | 2 | 2 | 5.0% | 3.95E-4 | 77866 |
[ |
GFA1 | Glutamine-fructose-6-phosphate amidotransferase, catalyzes the formation of glucosamine-6-P and glut... | 11 | 11 | 24.5% | 0.00212 | 80047 |
[ |
GRS1 | Cytoplasmic and mitochondrial glycyl-tRNA synthase that ligates glycine to the cognate anticodon bea... | 6 | 8 | 14.1% | 0.00166 | 75411 |
[ |
GUS1 | Glutamyl-tRNA synthetase (GluRS), forms a complex with methionyl-tRNA synthetase (Mes1p) and Arc1p; ... | 6 | 8 | 12.3% | 0.00156 | 80843 |
[ |
HIS4 | Multifunctional enzyme containing phosphoribosyl-ATP pyrophosphatase, phosphoribosyl-AMP cyclohydrol... | 3 | 4 | 5.8% | 6.93E-4 | 87721 |
[ |
HSK3 | Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced... | 4 | 4 | 66.7% | 0.00802 | 8088 |
[ |
ILV1 | Threonine deaminase, catalyzes the first step in isoleucine biosynthesis; expression is under genera... | 3 | 4 | 10.1% | 9.61E-4 | 63831 |
[ |
ILV2 | Acetolactate synthase, catalyses the first common step in isoleucine and valine biosynthesis and is ... | 6 | 6 | 21.5% | 0.00121 | 74937 |
[ |
KAP123 | Karyopherin beta, mediates nuclear import of ribosomal proteins prior to assembly into ribosomes and... | 7 | 9 | 13.5% | 0.00112 | 122601 |
[ |
KAR2 | ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding i... | 2 | 2 | 4.7% | 4.06E-4 | 74468 |
[ |
MES1 | Methionyl-tRNA synthetase, forms a complex with glutamyl-tRNA synthetase (Gus1p) and Arc1p, which in... | 2 | 2 | 3.9% | 3.69E-4 | 85678 |
[ |
MET5 | Sulfite reductase beta subunit, involved in amino acid biosynthesis, transcription repressed by meth... | 2 | 2 | 3.7% | 1.92E-4 | 161218 |
[ |
MIR1 | Mitochondrial phosphate carrier, imports inorganic phosphate into mitochondria; functionally redunda... | 4 | 5 | 30.2% | 0.00223 | 32812 |
[ |
NDE1 | Mitochondrial external NADH dehydrogenase, a type II NAD(P)H:quinone oxidoreductase that catalyzes t... | 2 | 2 | 7.7% | 4.94E-4 | 62774 |
[ |
NOP58 | Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the ... | 2 | 3 | 6.8% | 8.13E-4 | 56956 |
[ |
PDC1 | Major of three pyruvate decarboxylase isozymes, key enzyme in alcoholic fermentation, decarboxylates... | 4 | 5 | 15.5% | 0.00123 | 61495 |
[ |
PET9 | Major ADP/ATP carrier of the mitochondrial inner membrane, exchanges cytosolic ADP for mitochondrial... | 2 | 3 | 12.9% | 0.00131 | 34426 |
[ |
PFK1 | Alpha subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaer... | 2 | 2 | 4.1% | 2.8E-4 | 107970 |
[ |
PMA1 | Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plas... | 3 | 4 | 7.7% | 6.03E-4 | 99619 |
[ |
PMA2 | Plasma membrane H+-ATPase, isoform of Pma1p, involved in pumping protons out of the cell; regulator ... | 3 | 4 | 7.5% | 5.85E-4 | 102172 |
[ |
PSA1 | GDP-mannose pyrophosphorylase (mannose-1-phosphate guanyltransferase), synthesizes GDP-mannose from ... | 17 | 30 | 48.8% | 0.0115 | 39566 |
[ |
PYC1 | Pyruvate carboxylase isoform, cytoplasmic enzyme that converts pyruvate to oxaloacetate; highly simi... | 3 | 3 | 3.1% | 3.53E-4 | 130099 |
[ |
PYC2 | Pyruvate carboxylase isoform, cytoplasmic enzyme that converts pyruvate to oxaloacetate; highly simi... | 3 | 3 | 3.1% | 3.52E-4 | 130167 |
[ |
RPP0 | Conserved ribosomal protein P0 similar to rat P0, human P0, and E. coli L10e; shown to be phosphoryl... | 2 | 2 | 11.2% | 8.87E-4 | 33717 |
[ |
RPT4 | One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation ... | 3 | 3 | 10.3% | 9.5E-4 | 49408 |
[ |
RPT6 | One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation ... | 2 | 2 | 7.7% | 6.84E-4 | 45272 |
[ |
SES1 | Cytosolic seryl-tRNA synthetase, class II aminoacyl-tRNA synthetase that aminoacylates tRNA(Ser), di... | 2 | 3 | 6.7% | 8.99E-4 | 53310 |
[ |
SPC19 | Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced... | 23 | 51 | 82.4% | 0.0428 | 18910 |
[ |
SPC34 | Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced... | 25 | 67 | 69.8% | 0.0314 | 34078 |
[ |
SPC42 | Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate att... | 2 | 2 | 9.1% | 7.63E-4 | 42271 |
[ |
TEF1, TEF2 | Translational elongation factor EF-1 alpha; also encoded by TEF2; functions in the binding reaction ... | 15 | 25 | 33.2% | 0.00756 | 50033 |
[ |
TFP1 | Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein prec... | 11 | 14 | 17.2% | 0.00181 | 118637 |
[ |
THS1 | Threonyl-tRNA synthetase, essential cytoplasmic protein | 4 | 5 | 8.3% | 9.43E-4 | 84520 |
[ |
TUB1 | Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form... | 3 | 5 | 11.6% | 0.00155 | 49800 |
[ |
TUB2 | Beta-tubulin; associates with alpha-tubulin (Tub1p and Tub3p) to form tubulin dimer, which polymeriz... | 3 | 8 | 15.1% | 0.00242 | 50923 |
[ |
URA2 | Bifunctional carbamoylphosphate synthetase (CPSase)-aspartate transcarbamylase (ATCase), catalyzes t... | 10 | 10 | 8.8% | 6.25E-4 | 245123 |
[ |
URA7 | Major CTP synthase isozyme (see also URA8), catalyzes the ATP-dependent transfer of the amide nitrog... | 2 | 2 | 6.0% | 4.78E-4 | 64710 |
[ |
VMA2 | Subunit B of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), a... | 10 | 13 | 31.1% | 0.00348 | 57749 |
[ |
YBR012W-B | Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... | 2 | 2 | 1.8% | 1.58E-4 | 198868 |
[ |
YDR098C-B | Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... | 2 | 2 | 1.8% | 1.58E-4 | 198594 |
[ |
YDR210C-D | Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... | 2 | 2 | 1.8% | 1.58E-4 | 198838 |
[ |
YDR261C-D | Retrotransposon TYA Gag and TYB Pol genes; in YDRCTY1-3 TYB is mutant and probably non-functional | 2 | 2 | 1.9% | 1.73E-4 | 181660 |
[ |
YDR316W-B | Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... | 2 | 2 | 1.8% | 1.58E-4 | 198526 |
[ |
YDR365W-B | Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... | 2 | 2 | 1.8% | 1.58E-4 | 198658 |
[ |
YEF3 | Translational elongation factor 3, stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes b... | 12 | 15 | 26.1% | 0.00199 | 115945 |
[ |
YER138C | Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... | 2 | 2 | 1.8% | 1.58E-4 | 198561 |
[ |
YER160C | Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... | 2 | 2 | 1.8% | 1.58E-4 | 198568 |
[ |
YGR027W-B | Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... | 2 | 2 | 1.8% | 1.58E-4 | 198656 |
[ |
YGR038C-B | Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... | 2 | 2 | 1.8% | 1.58E-4 | 198520 |
[ |
YHB1 | Nitric oxide oxidoreductase, flavohemoglobin involved in nitric oxide detoxification; plays a role i... | 4 | 4 | 22.8% | 0.00139 | 44646 |
[ |
YHR020W | Protein of unknown function that may interact with ribosomes, based on co-purification experiments; ... | 6 | 8 | 13.2% | 0.00161 | 77386 |