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View Protein Complex Details

Complex Overview

From Publication: Hazbun TR, et al. (2003) Assigning function to yeast proteins by integration of technologies. Mol Cell 12(6):1353-65
Notes: Complex description from publication: 12. YDL209C (CWC2) mRNA splicing
Complex Size: 25 proteins

Complex Member Proteins

Protein

GO Cellular Component GO Biological Process GO Molecular Function
BRR2
  • U4/U6 x U5 tri-snRNP complex
  • U5 snRNP
  • RNA splicing
  • mRNA metabolic process
  • auxin biosynthetic process
  • mRNA processing
  • spliceosome conformational change to release U4 (or U4atac) and U1 (or U11)
  • ATP-dependent RNA helicase activity
  • CDC40
  • spliceosomal complex
  • nuclear mRNA branch site recognition
  • nuclear mRNA 3'-splice site recognition
  • second spliceosomal transesterification activity
  • CEF1
  • spliceosomal complex
  • nuclear mRNA splicing, via spliceosome
  • DNA binding
  • first spliceosomal transesterification activity
  • CLF1
  • chromatin
  • DNA replication
  • cell cycle
  • cis assembly of pre-catalytic spliceosome
  • nuclear mRNA splicing, via spliceosome
  • protein binding
  • CWC2
  • spliceosomal complex
  • nuclear mRNA splicing, via spliceosome
  • molecular_function
  • CWC22
  • spliceosomal complex
  • nuclear mRNA splicing, via spliceosome
  • molecular_function
  • CWC23
  • spliceosomal complex
  • biological_process
  • molecular_function
  • ECM2
  • nucleus
  • cellular cell wall organization
  • nuclear mRNA splicing, via spliceosome
  • molecular_function
  • ISY1
  • spliceosomal complex
  • nuclear mRNA splicing, via spliceosome
  • second spliceosomal transesterification activity
  • first spliceosomal transesterification activity
  • LEA1
  • cytoplasm
  • U2 snRNP
  • telomere maintenance
  • nuclear mRNA splicing, via spliceosome
  • RNA binding
  • molecular_function
  • NTR2
  • nucleus
  • cytoplasm
  • spliceosomal complex
  • endoplasmic reticulum
  • nuclear mRNA splicing, via spliceosome
  • molecular_function
  • PRP19
  • nucleus
  • cytoplasm
  • mitochondrion
  • spliceosomal complex
  • nuclear mRNA splicing, via spliceosome
  • ubiquitin-dependent protein catabolic process
  • ubiquitin-protein ligase activity
  • first spliceosomal transesterification activity
  • RNA binding
  • PRP43
  • mitochondrion
  • spliceosomal complex
  • nuclear outer membrane
  • maturation of SSU-rRNA
  • ribosomal large subunit biogenesis
  • ribosome biogenesis
  • rRNA processing
  • processing of 27S pre-rRNA
  • ATP-dependent RNA helicase activity
  • PRP45
  • nucleus
  • spliceosomal complex
  • positive regulation of transcription from RNA polymerase II promoter
  • nuclear mRNA splicing, via spliceosome
  • transcription activator activity
  • PRP46
  • spliceosomal complex
  • nuclear mRNA splicing, via spliceosome
  • molecular_function
  • PRP8
  • U4/U6 x U5 tri-snRNP complex
  • U5 snRNP
  • nuclear mRNA 3'-splice site recognition
  • nuclear mRNA splicing, via spliceosome
  • U5 snRNA binding
  • SMB1
  • U4/U6 x U5 tri-snRNP complex
  • U5 snRNP
  • U1 snRNP
  • nuclear mRNA splicing, via spliceosome
  • RNA binding
  • molecular_function
  • SMD1
  • commitment complex
  • U4/U6 x U5 tri-snRNP complex
  • U5 snRNP
  • U1 snRNP
  • nuclear mRNA splicing, via spliceosome
  • mRNA binding
  • SMD3
  • commitment complex
  • U4/U6 x U5 tri-snRNP complex
  • U5 snRNP
  • U1 snRNP
  • nuclear mRNA splicing, via spliceosome
  • mRNA binding
  • SNT309
  • spliceosomal complex
  • nuclear mRNA splicing, via spliceosome
  • protein binding
  • SNU114
  • U4/U6 x U5 tri-snRNP complex
  • U5 snRNP
  • generation of catalytic spliceosome for first transesterification step
  • nuclear mRNA splicing, via spliceosome
  • GTP binding
  • U5 snRNA binding
  • GTPase activity
  • SPP382
  • nucleus
  • cytoplasm
  • mitochondrion
  • spliceosomal complex
  • nuclear mRNA splicing, via spliceosome
  • RNA binding
  • SYF1
  • spliceosomal complex
  • cell cycle
  • nuclear mRNA splicing, via spliceosome
  • molecular_function
  • SYF2
  • spliceosomal complex
  • cell cycle
  • nuclear mRNA splicing, via spliceosome
  • second spliceosomal transesterification activity
  • first spliceosomal transesterification activity
  • YJU2
  • nucleus
  • nuclear mRNA splicing, via spliceosome
  • molecular_function
  • Cellular Component Analysis

    Given the number of proteins in the complex (A), total proteins annotated with a given GO term (B), and the total number of annotated proteins (T); the p-value represents the chances of randomly having the number of proteins in the complex annotated with a specific GO term (I).

    Only showing terms with a p-value less than or equal to 0.01.

    GO Term

    P-value

    A

    B

    I

    T

    spliceosomal complex 1.1042E-32 25 45 17 6292
    ribonucleoprotein complex 1.3993E-21 25 514 22 6292
    nuclear part 2.5984E-14 25 1103 22 6292
    U5 snRNP 6.0589E-12 25 14 6 6292
    nucleus 2.9033E-11 25 2041 24 6292
    macromolecular complex 1.1811E-10 25 1635 22 6292
    U4/U6 x U5 tri-snRNP complex 1.7448E-9 25 32 6 6292
    small nuclear ribonucleoprotein complex 2.9921E-9 25 63 7 6292
    organelle part 8.8641E-9 25 2282 23 6292
    intracellular organelle part 8.8641E-9 25 2282 23 6292
    intracellular organelle 1.8147E-5 25 4070 25 6292
    organelle 1.8259E-5 25 4071 25 6292
    U1 snRNP 3.633E-5 25 17 3 6292
    membrane-bounded organelle 4.7722E-5 25 3771 24 6292
    intracellular membrane-bounded organelle 4.7722E-5 25 3771 24 6292
    commitment complex 9.7633E-4 25 12 2 6292
    U2-type spliceosomal complex 9.7633E-4 25 12 2 6292
    intracellular part 2.3087E-3 25 4938 25 6292
    intracellular 2.7836E-3 25 4975 25 6292

    Biological Process Analysis

    Given the number of proteins in the complex (A), total proteins annotated with a given GO term (B), and the total number of annotated proteins (T); the p-value represents the chances of randomly having the number of proteins in the complex annotated with a specific GO term (I).

    Only showing terms with a p-value less than or equal to 0.01.

    GO Term

    P-value

    A

    B

    I

    T

    nuclear mRNA splicing, via spliceosome 6.4105E-41 25 99 23 6292
    RNA splicing, via transesterification reactions with bulged adenosine as nucleophile 8.3227E-41 25 100 23 6292
    RNA splicing, via transesterification reactions 4.7811E-40 25 107 23 6292
    RNA splicing 9.9285E-38 25 132 23 6292
    mRNA processing 6.3917E-36 25 156 23 6292
    mRNA metabolic process 1.3018E-32 25 213 23 6292
    RNA processing 6.5222E-29 25 380 24 6292
    RNA metabolic process 3.646E-19 25 954 24 6292
    gene expression 4.5341E-16 25 1283 24 6292
    nucleic acid metabolic process 4.7316E-15 25 1415 24 6292
    nucleobase, nucleoside, nucleotide and nucleic acid metabolic process 5.3353E-14 25 1566 24 6292
    cellular nitrogen compound metabolic process 9.8691E-13 25 1770 24 6292
    nitrogen compound metabolic process 1.3065E-12 25 1791 24 6292
    cellular macromolecule metabolic process 4.1889E-10 25 2285 24 6292
    macromolecule metabolic process 8.0334E-10 25 2349 24 6292
    primary metabolic process 1.0865E-7 25 2896 24 6292
    cellular metabolic process 3.185E-7 25 3033 24 6292
    metabolic process 8.0676E-7 25 3157 24 6292
    nuclear mRNA 3'-splice site recognition 1.5158E-5 25 2 2 6292
    ribonucleoprotein complex assembly 2.5263E-5 25 92 5 6292
    spliceosome assembly 4.3481E-5 25 18 3 6292
    mRNA splice site selection 2.2516E-4 25 6 2 6292
    spliceosomal conformational changes to generate catalytic conformation 5.3645E-4 25 9 2 6292
    cellular macromolecular complex assembly 6.3578E-4 25 182 5 6292
    cellular process 1.7226E-3 25 4426 24 6292
    ribonucleoprotein complex biogenesis 2.8541E-3 25 374 6 6292
    cellular macromolecular complex subunit organization 3.068E-3 25 259 5 6292
    regulation of hormone levels 3.9733E-3 25 1 1 6292
    hormone metabolic process 3.9733E-3 25 1 1 6292
    hormone biosynthetic process 3.9733E-3 25 1 1 6292
    spliceosome conformational change to release U4 (or U4atac) and U1 (or U11) 3.9733E-3 25 1 1 6292
    auxin biosynthetic process 3.9733E-3 25 1 1 6292
    auxin metabolic process 3.9733E-3 25 1 1 6292
    macromolecular complex assembly 4.3602E-3 25 281 5 6292
    cis assembly of pre-catalytic spliceosome 7.9314E-3 25 2 1 6292

    Molecular Function Analysis

    Given the number of proteins in the complex (A), total proteins annotated with a given GO term (B), and the total number of annotated proteins (T); the p-value represents the chances of randomly having the number of proteins in the complex annotated with a specific GO term (I).

    Only showing terms with a p-value less than or equal to 0.01.

    GO Term

    P-value

    A

    B

    I

    T

    first spliceosomal transesterification activity 2.8929E-9 25 6 4 6292
    second spliceosomal transesterification activity 1.9197E-6 25 7 3 6292
    U5 snRNA binding 1.5158E-5 25 2 2 6292
    RNA binding 5.58E-5 25 367 8 6292
    snRNA binding 1.5047E-4 25 5 2 6292
    nucleic acid binding 6.7592E-4 25 666 9 6292
    ATP-dependent RNA helicase activity 5.3782E-3 25 28 2 6292
    RNA-dependent ATPase activity 5.7626E-3 25 29 2 6292
    binding 6.8185E-3 25 1294 11 6292
    mRNA binding 8.3231E-3 25 35 2 6292

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle