Protein Overview: RPB8

General Information:
Name(s) found:	RPB8 / YOR224C [SGD]
Description(s) found: Found 118 descriptions. SHOW ALL	RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III [SGD]
Organism:	Saccharomyces cerevisiae
Length:	146 amino acids
Gene Ontology:
Cellular Component:	DNA-directed RNA polymerase I complex [TAS] DNA-directed RNA polymerase III complex [TAS] DNA-directed RNA polymerase II, core complex [TAS]
Biological Process:	transcription from RNA polymerase III promoter [TAS] transcription from RNA polymerase I promoter [TAS] transcription from RNA polymerase II promoter [TAS]
Molecular Function:	DNA-directed RNA polymerase activity [TAS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSNTLFDDIF QVSEVDPGRY NKVCRIEAAS TTQDQCKLTL DINVELFPVA AQDSLTVTIA  60
   61 SSLNLEDTPA NDSSATRSWR PPQAGDRSLA DDYDYVMYGT AYKFEEVSKD LIAVYYSFGG 120
  121 LLMRLEGNYR NLNNLKQENA YLLIRR

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		DST1 IWR1 RET1 RPA12 RPA135 RPA14 RPA190 RPA34 RPA43 RPA49 RPB10 RPB11 RPB2 RPB3 RPB4 RPB5 RPB7 RPB8 RPB9 RPC10 RPC11 RPC17 RPC19 RPC25 RPC31 RPC34 RPC37 RPC40 RPC53 RPC82 RPO21 RPO26 RPO31 SPT4 SPT5 TAF14 TFG1 TFG2
View Details	(MIPS) Mewes HW, et al. (2004)		CDC73 RET1 RPA12 RPA135 RPA14 RPA190 RPA34 RPA43 RPA49 RPB10 RPB11 RPB2 RPB3 RPB4 RPB5 RPB7 RPB8 RPB9 RPC10 RPC19 RPC25 RPC31 RPC34 RPC40 RPC82 RPO21 RPO26 RPO31
View Details	Krogan NJ, et al. (2006)		ALG3 BUD27 CBF2 DST1 ESS1 IWR1 RPB10 RPB11 RPB2 RPB3 RPB4 RPB5 RPB7 RPB8 RPB9 RPO21 RPO26 SPT4 TAF14 TFG1 TFG2 YBL059W YBR259W YPL225W
View Details	Gavin AC, et al. (2002)		ABD1 AMS1 AOS1 ASC1 CDC14 DHH1 FBA1 IWR1 MAF1 RET1 RPA12 RPA135 RPA190 RPA43 RPA49 RPB10 RPB11 RPB2 RPB3 RPB4 RPB5 RPB7 RPB8 RPB9 RPC11 RPC17 RPC19 RPC25 RPC31 RPC34 RPC37 RPC40 RPC53 RPC82 RPO21 RPO26 RPO31 RSC8 SPT4 SPT5 SRO7 TAF14 TFG1 TFG2
View Details	Qiu et al. (2008)		RET1 RPA12 RPA135 RPA14 RPA190 RPA34 RPA43 RPA49 RPB10 RPB11 RPB2 RPB3 RPB4 RPB5 RPB7 RPB8 RPB9 RPC10 RPC11 RPC17 RPC19 RPC25 RPC31 RPC34 RPC37 RPC40 RPC53 RPC82 RPO21 RPO26 RPO31 TAF14

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	RPA135	his-HA tag on RPA135	Schneider, DA, et al. (2006)
View Run	RPA135	his-HA tag on RPA135	Schneider, DA, et al. (2006)
View Run	RPA135	his-HA tag on RPA135	Schneider, DA, et al. (2006)
View Run	RPA135	his-HA tag on RPA135	Schneider, DA, et al. (2006)
View Run	CEP3	sample cbf3 from feb 2005	Sandall S, et all (2006)
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	SNF4	RPB8	1	Unpublished Fields Lab Data
View Screen	CCE1	RPB8	1	Unpublished Fields Lab Data

Microscopy / Localization Data

New Feature: Upload Your Own Microscopy Data

	PROTEIN(S)	PUBLICATION
View Data	RPB8	Huh WK, et al. (2003)

Protein Structure Data

[What does the above image mean?]
[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..146]	PSI-BLAST	58.69897	YEAST RNA POLYMERASE SUBUNIT RPB8, NMR, MINIMIZED AVERAGE STRUCTURE, ALPHA CARBONS ONLY

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2