Protein Overview: NUP116

General Information:
Name(s) found:	NUP116 / YMR047C [SGD]
Description(s) found: Found 27 descriptions. SHOW ALL	Subunit of the Nup82 subcomplex of the nuclear pore complex; localized to both sides of the pore; contains a repetitive GLFG motif that interacts with mRNA export factor Mex67p and with karyopherin Kap95p; homologous to Nup100p [SGD]
Organism:	Saccharomyces cerevisiae
Length:	1113 amino acids
Gene Ontology:
Cellular Component:	nuclear pore [IDA]
Biological Process:	mRNA export from nucleus [IMP] rRNA export from nucleus [TAS] nuclear pore organization [TAS] snRNP protein import into nucleus [TAS] mRNA-binding (hnRNP) protein import into nucleus [TAS] snRNA export from nucleus [TAS] protein import into nucleus [IMP] tRNA export from nucleus [IMP ] NLS-bearing substrate import into nucleus [TAS] tRNA splicing, via endonucleolytic cleavage and ligation [IMP] protein export from nucleus [TAS] ribosomal protein import into nucleus [TAS]
Molecular Function:	structural molecule activity [TAS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MFGVSRGAFP SATTQPFGST GSTFGGQQQQ QQPVANTSAF GLSQQTNTTQ APAFGNFGNQ  60
   61 TSNSPFGMSG STTANGTPFG QSQLTNNNAS GSIFGGMGNN TALSAGSASV VPNSTAGTSI 120
  121 KPFTTFEEKD PTTGVINVFQ SITCMPEYRN FSFEELRFQD YQAGRKFGTS QNGTGTTFNN 180
  181 PQGTTNTGFG IMGNNNSTTS ATTGGLFGQK PATGMFGTGT GSGGGFGSGA TNSTGLFGSS 240
  241 TNLSGNSAFG ANKPATSGGL FGNTTNNPTN GTNNTGLFGQ QNSNTNGGLF GQQQNSFGAN 300
  301 NVSNGGAFGQ VNRGAFPQQQ TQQGSGGIFG QSNANANGGA FGQQQGTGAL FGAKPASGGL 360
  361 FGQSAGSKAF GMNTNPTGTT GGLFGQTNQQ QSGGGLFGQQ QNSNAGGLFG QNNQSQNQSG 420
  421 LFGQQNSSNA FGQPQQQGGL FGSKPAGGLF GQQQGASTFA SGNAQNNSIF GQNNQQQQST 480
  481 GGLFGQQNNQ SQSQPGGLFG QTNQNNNQPF GQNGLQQPQQ NNSLFGAKPT GFGNTSLFSN 540
  541 STTNQSNGIS GNNLQQQSGG LFQNKQQPAS GGLFGSKPSN TVGGGLFGNN QVANQNNPAS 600
  601 TSGGLFGSKP ATGSLFGGTN STAPNASSGG IFGSNNASNT AATTNSTGLF GNKPVGAGAS 660
  661 TSAGGLFGNN NNSSLNNSNG STGLFGSNNT SQSTNAGGLF QNNTSTNTSG GGLFSQPSQS 720
  721 MAQSQNALQQ QQQQQRLQIQ NNNPYGTNEL FSKATVTNTV SYPIQPSATK IKADERKKAS 780
  781 LTNAYKMIPK TLFTAKLKTN NSVMDKAQIK VDPKLSISID KKNNQIAISN QQEENLDESI 840
  841 LKASELLFNP DKRSFKNLIN NRKMLIASEE KNNGSQNNDM NFKSKSEEQE TILGKPKMDE 900
  901 KETANGGERM VLSSKNDGED SATKHHSRNM DEENKENVAD LQKQEYSEDD KKAVFADVAE 960
  961 KDASFINENY YISPSLDTLS SYSLLQLRKV PHLVVGHKSY GKIEFLEPVD LAGIPLTSLG1020
 1021 GVIITFEPKT CIIYANLPNR PKRGEGINVR ARITCFNCYP VDKSTRKPIK DPNHQLVKRH1080
 1081 IERLKKNPNS KFESYDADSG TYVFIVNHAA EQT

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		NSP1 NUP116
View Details	(MIPS) Mewes HW, et al. (2004)		GLE2 NOP14 NUP1 NUP100 NUP116 NUP133 NUP157 NUP159 NUP170 NUP188 NUP2 NUP42 POM152
View Details	Gavin AC, et al. (2002)		MRPL1 MRPL15 MRPL24 MRPL3 MRPL35 MRPL8 NIC96 NSP1 NUP116 NUP159 NUP82 YML6
View Details	Qiu et al. (2008)		ASM4 GLE2 KAP120 KAP123 MEX67 NDC1 NIC96 NSP1 NUP1 NUP100 NUP116 NUP120 NUP133 NUP145 NUP157 NUP159 NUP170 NUP188 NUP2 NUP42 NUP49 NUP53 NUP57 NUP60 NUP82 NUP84 POM152 SEC13 SEH1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	RPA135	his-HA tag on RPA135	Schneider, DA, et al. (2006)
View Run	RPA135	his-HA tag on RPA135	Schneider, DA, et al. (2006)
View Run	ASF1	3829 - low filter	Green EM, et al (2005)
View Run	RLF2	phosphorylation data - cascade search	Green EM, et al (2005)
View Run	MLP2	#29 Asynchronous Prep5-TiO2 Flowthrough	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	FAR10	NUP116	1	Unpublished Fields Lab Data
View Screen	PDI1	NUP116	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	NUP116	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..510]	MSA	12.136996	View MSA. No confident structure predictions are available.
2	View Details	[511..623]	deduced	N/A	No confident structure predictions are available.
3	View Details	[624..1006]	MSA	7.152997	View MSA. No confident structure predictions are available.
4	View Details	[1007..1113]	deduced	N/A	No confident structure predictions are available.
5	View Details	[683..781]	MSA	2.300996	View MSA. No confident structure predictions are available.
6	View Details	[782..967]	deduced	N/A	No confident structure predictions are available.
7	View Details	[968..1113]	PSI-BLAST	64.154902	Multiple conformations in the ligand-binding site of the yeast nuclear pore targeting domain of NUP116P

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.80

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2