Protein Overview: APC2

General Information:
Name(s) found:	APC2 / YLR127C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|995689\|emb\|CAA62638.1\| L3105 [Saccharomyces cerevisiae] [NCBI NR] gi\|6323156\|ref\|NP_013228.1\| Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; similar to c [NCBI NR] gi\|55976297\|sp\|Q12440.1\|APC2_YEAST RecName: Full=Anaphase-promoting complex subunit 2 [NCBI NR] pir\|\|S59315 hypothetical protein YLR127c - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|1360535\|emb\|CAA97696.1\| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR] gi\|1297041\|emb\|CAA61705.1\| L3108 [Saccharomyces cerevisiae] [NCBI NR] gi\|1256867\|gb\|AAB82373.1\| Rsi1p: Anaphase Promoting Complex (APC) component [Saccharomyces cerevisiae] [NCBI NR] Anaphase-promoting complex subunit 2 OS=Saccharomyces cerevisiae GN=APC2 PE=1 SV=1 [Swiss-Prot] APC2 Component of the anaphase promoting complex [mips] Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; similar to cullin Cdc53p [SGD] APC2, Chr XII from 395759-398320, reverse complement [yeast_orfs2] Chr XII from 395759-398320, reverse complement [yeastorf3.fasta] Chr XII from 398320-395759, reverse complement [yeastorf5.fasta] >Q12440 (Q12440) ORF YLR127C [4932.FASTACCERV.txt] APC2 SGDID:S0004117, Chr XII from 398320-395759, reverse complement, Verified ORF [yeastflysequest.fasta] Apc2p [Saccharomyces cerevisiae]gi\|1297041\|emb\|CAA61705.1\| L3108 [Saccharomyces cerevisiae]gi\|1360535\|emb\|CAA97696.1\| unnamed protein product [Saccharomyces cerevisiae]gi\|995689\|emb\|CAA62638.1\| L3105 [Saccharomyces cerevisiae]gi\|1256867\|gb\|AAB82373.1\| [nrdb_11152004_Chlamydomonas] (Q12440) L3108 protein (S.cerevisiae chromosome XII reading frame ORF YLR127c) (Rsi1p: Anaphase Promoting Complex (APC) component) (L3105 protein) [cerv4932.FASTAC] APC2 SGDID:S000004117, Chr XII from 398320-395759, reverse complement, Verified ORF, "Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cycli [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] Rsi1p: Anaphase Promoting Complex (APC) component [Saccharomyces cerevisiae] [nr-271106-contam.fasta] L3105 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Anaphase-promoting complex subunit 2 [nr-271106-contam.fasta] L3108 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] unnamed protein product [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; similar to cullin Cdc53p; Apc2p [Sacchar [nr-271106-contam.fasta] APC2 SGDID:S000004117, Chr XII from 398320-395759, reverse complement, Verified ORF, "Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; similar to cullin Cdc53p" [yeast-bovrabprots.fasta] [chemostatdb.fasta] Anaphase-promoting complex subunit 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=APC2 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] APC2 SGDID:S000004117, Chr XII from 398319-395758, Genome Release 64-1-1, reverse complement, Verified ORF, "Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the catalytic core of the APC/C; has similarity to cullin Cdc53p" [yeast-030211-contam.fasta] APC2 SGDID:S000004117, Chr XII from 398319-395758, Genome Release 64-2-1, reverse complement, Verified ORF, "Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the catalytic core of the APC/C; has similarity to cullin Cdc53p" [20141208_orf_trans.fasta] YLR127C SGDID:S000004117, Chr XII from 398319-395758, Genome Release 64-1-1, reverse complement, Verified ORF, "Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the catalytic core of the APC/C; has similarity to cullin Cdc53p" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	853 amino acids
Gene Ontology:
Cellular Component:	anaphase-promoting complex [TAS]
Biological Process:	mitotic spindle elongation [TAS] cyclin catabolic process [TAS] mitotic sister chromatid segregation [TAS] anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process [IPI] mitotic metaphase/anaphase transition [TAS] protein ubiquitination [IPI]
Molecular Function:	ubiquitin-protein ligase activity [TAS] protein binding [TAS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSFQITPTRD LKVITDELQT LSSYIFHTNI VDDLNSLLTW MSPNDAKSNH QLRPPSLRIK  60
   61 NIIKVLFPNN ATTSPYSMIN TSQANNSIVN EGNTNKELQL QLFSTLKEFY IFQVRYHFFL 120
  121 HFNNINYLKD IQRWENYYEF PLRYVPIFDV NVNDWALELN SLRHYLLNRN IKFKNNLRTR 180
  181 LDKLIMDDDF DLADNLIQWL KSANGSLSST ELIVNALYSK INKFCEDNMS RVWNKRFMIM 240
  241 ETFNKFINQY WSQFSKLVGC PEDDHELTTT VFNCFESNFL RIRTNEIFDI CVLAYPDSKV 300
  301 TLLELRKIMK DFKDYTNIVT TFLSDFKKYI LNPSVTTVDA LLRYVKTIKA FLVLDPTGRC 360
  361 LHSITTFVKP YFQERKHLVN VLLYAMLDLP EEELKEKINF NVDMKALLSL VDTLHDSDIN 420
  421 QDTNITKRDK NKKSPFLWNL KVKGKRELNK DLPIRHAMLY EHILNYYIAW VPEPNDMIPG 480
  481 NIKSSYIKTN LFEVLLDLFE SREFFISEFR NLLTDRLFTL KFYTLDEKWT RCLKLIREKI 540
  541 VKFTETSHSN YITNGILGLL ETTAPAADAD QSNLNSIDVM LWDIKCSEEL CRKMHEVAGL 600
  601 DPIIFPKFIS LLYWKYNCDT QGSNDLAFHL PIDLERELQK YSDIYSQLKP GRKLQLCKDK 660
  661 GKVEIQLAFK DGRKLVLDVS LEQCSVINQF DSPNDEPICL SLEQLSESLN IAPPRLTHLL 720
  721 DFWIQKGVLL KENGTYSVIE HSEMDFDQAQ KTAPMEIENS NYELHNDSEI ERKYELTLQR 780
  781 SLPFIEGMLT NLGAMKLHKI HSFLKITVPK DWGYNRITLQ QLEGYLNTLA DEGRLKYIAN 840
  841 GSYEIVKNGH KNS

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		APC1 APC2 APC4 APC5 CDC16 CDC23 CDC27 DOC1
View Details	(MIPS) Mewes HW, et al. (2004)		APC1 APC11 APC2 APC4 APC5 APC9 CDC16 CDC23 CDC26 CDC27 DOC1
View Details	Krogan NJ, et al. (2006)		APC1 APC2 APC4 APC5 CDC16 CDC23 CDC26 CDC27 DOC1 FMP52 MND2 SSP2
View Details	Gavin AC, et al. (2006)		APC1 APC2 APC4 APC5 APC9 CDC16 CDC23 CDC27
View Details	Gavin AC, et al. (2002)		ACT1 APC1 APC2 CDC16 CDC23 CDC27
View Details	Ho Y, et al. (2002)		APC2 FMP48 HIS4
View Details	Qiu et al. (2008)		APC1 APC11 APC2 APC4 APC5 APC9 BUL2 CDC16 CDC23 CDC26 CDC27 DOC1 SWM1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	CEP3	sample cbf3 from feb 2005	Sandall S, et all (2006)
View Run	MLP2	#03 Alpha Factor Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	APC2	MND2	2	Unpublished Fields Lab Data
View Screen	APC2	SOR1	2	Unpublished Fields Lab Data
View Screen	APC2	WTM2	2	Unpublished Fields Lab Data
View Screen	APC2	KGD2	2	Unpublished Fields Lab Data
View Screen	APC2	NUP57	2	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	APC2	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..93]	deduced	N/A	Confident ab initio structure predictions are available.
2	View Details	[94..186]	MSA	1.05099	View MSA. Confident ab initio structure predictions are available.
3	View Details	[187..406]	MSA	2.071982	View MSA. No confident structure predictions are available.
4	View Details	[407..758]	deduced	N/A	No confident structure predictions are available.
5	View Details	[759..853]	PSI-BLAST	375.64593	Anaphase promoting complex (APC)

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
ubiquitin-protein ligase activity	9.47178230940453	bayes_pls_golite062009
small conjugating protein ligase activity	9.15192834197166	bayes_pls_golite062009
acid-amino acid ligase activity	8.12051073361876	bayes_pls_golite062009
ligase activity, forming carbon-nitrogen bonds	6.87003086224111	bayes_pls_golite062009
ligase activity	4.85660414833673	bayes_pls_golite062009
transcription regulator activity	4.44907471960058	bayes_pls_golite062009
transcription factor activity	3.48782936574155	bayes_pls_golite062009
transcription repressor activity	2.39237599101586	bayes_pls_golite062009
binding	2.3219000606044	bayes_pls_golite062009
transcription activator activity	1.79615705315806	bayes_pls_golite062009
nucleic acid binding	1.31024813110778	bayes_pls_golite062009
RNA polymerase II transcription factor activity	1.2152419300113	bayes_pls_golite062009
protein binding	1.15192193279649	bayes_pls_golite062009
DNA binding	1.05574145691051	bayes_pls_golite062009
vasopressin activated calcium mobilizing receptor activity	0.416181464241621	bayes_pls_golite062009
transcription factor binding	0.31161141447305	bayes_pls_golite062009
catalytic activity	0.201606344181603	bayes_pls_golite062009
hydrolase activity	0.140191206949582	bayes_pls_golite062009
specific transcriptional repressor activity	0.0565989703351002	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2