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Protein Overview: APC2

General Information:
Name(s) found: APC2 / YLR127C [SGD]
Description(s) found:
Found 30 descriptions.
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  • Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; similar to cullin Cdc53p [SGD]
    • Organism: Saccharomyces cerevisiae
    Length: 853 amino acids

    Gene Ontology:
    Cellular Component: anaphase-promoting complex [TASLink to PubMed article]
    Biological Process: mitotic spindle elongation [TASLink to PubMed article]
    cyclin catabolic process [TASLink to PubMed article]
    mitotic sister chromatid segregation [TASLink to PubMed article]
    anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process [IPILink to PubMed article]
    mitotic metaphase/anaphase transition [TASLink to PubMed article]
    protein ubiquitination [IPILink to PubMed article]
    Molecular Function: ubiquitin-protein ligase activity [TASLink to PubMed article]
    protein binding [TASLink to PubMed article]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSFQITPTRD LKVITDELQT LSSYIFHTNI VDDLNSLLTW MSPNDAKSNH QLRPPSLRIK  60
   61 NIIKVLFPNN ATTSPYSMIN TSQANNSIVN EGNTNKELQL QLFSTLKEFY IFQVRYHFFL 120
  121 HFNNINYLKD IQRWENYYEF PLRYVPIFDV NVNDWALELN SLRHYLLNRN IKFKNNLRTR 180
  181 LDKLIMDDDF DLADNLIQWL KSANGSLSST ELIVNALYSK INKFCEDNMS RVWNKRFMIM 240
  241 ETFNKFINQY WSQFSKLVGC PEDDHELTTT VFNCFESNFL RIRTNEIFDI CVLAYPDSKV 300
  301 TLLELRKIMK DFKDYTNIVT TFLSDFKKYI LNPSVTTVDA LLRYVKTIKA FLVLDPTGRC 360
  361 LHSITTFVKP YFQERKHLVN VLLYAMLDLP EEELKEKINF NVDMKALLSL VDTLHDSDIN 420
  421 QDTNITKRDK NKKSPFLWNL KVKGKRELNK DLPIRHAMLY EHILNYYIAW VPEPNDMIPG 480
  481 NIKSSYIKTN LFEVLLDLFE SREFFISEFR NLLTDRLFTL KFYTLDEKWT RCLKLIREKI 540
  541 VKFTETSHSN YITNGILGLL ETTAPAADAD QSNLNSIDVM LWDIKCSEEL CRKMHEVAGL 600
  601 DPIIFPKFIS LLYWKYNCDT QGSNDLAFHL PIDLERELQK YSDIYSQLKP GRKLQLCKDK 660
  661 GKVEIQLAFK DGRKLVLDVS LEQCSVINQF DSPNDEPICL SLEQLSESLN IAPPRLTHLL 720
  721 DFWIQKGVLL KENGTYSVIE HSEMDFDQAQ KTAPMEIENS NYELHNDSEI ERKYELTLQR 780
  781 SLPFIEGMLT NLGAMKLHKI HSFLKITVPK DWGYNRITLQ QLEGYLNTLA DEGRLKYIAN 840
  841 GSYEIVKNGH KNS

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details Riffle et al. (2010) (Unpublished Data) APC1 APC2 APC4 APC5 CDC16 CDC23 CDC27 DOC1
    View Details (MIPS) Mewes HW, et al. (2004) APC1 APC11 APC2 APC4 APC5 APC9 CDC16 CDC23 CDC26 CDC27 DOC1
    View Details Krogan NJ, et al. (2006) APC1 APC2 APC4 APC5 CDC16 CDC23 CDC26 CDC27 DOC1 FMP52 MND2 SSP2
    View Details Gavin AC, et al. (2006) APC1 APC2 APC4 APC5 APC9 CDC16 CDC23 CDC27
    View Details Gavin AC, et al. (2002) ACT1 APC1 APC2 CDC16 CDC23 CDC27
    View Details Ho Y, et al. (2002) APC2 FMP48 HIS4
    View Details Qiu et al. (2008) APC1 APC11 APC2 APC4 APC5 APC9 BUL2 CDC16 CDC23 CDC26 CDC27 DOC1 SWM1

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run CEP3 sample cbf3 from feb 2005 Sandall S, et all (2006)
    View Run MLP2 #03 Alpha Factor Prep1-TiO2 Flowthrough Keck JM, et al. (2011)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    SHOWING SINGLE HITS. [ Hide Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

      BAIT PREY HITS PUBLICATION
    View Screen APC2 SOR1 2 Unpublished Fields Lab Data
    View Screen APC2 WTM2 2 Unpublished Fields Lab Data
    View Screen APC2 KGD2 2 Unpublished Fields Lab Data
    View Screen APC2 NUP57 2 Unpublished Fields Lab Data
    View Screen APC2 MND2 2 Unpublished Fields Lab Data
    View Screen UBC11 APC2 1 Unpublished Fields Lab Data
    View Screen RAD51 APC2 1 Unpublished Fields Lab Data

    Microscopy / Localization Data

    New Feature: Upload Your Own Microscopy Data

      PROTEIN(S) PUBLICATION
    View Data APC2 Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..93] deduced N/A Confident ab initio structure predictions are available.
    2 View Details [94..186] MSA 1.05099 View MSA. Confident ab initio structure predictions are available.
    3 View Details [187..406] MSA 2.071982 View MSA. No confident structure predictions are available.
    4 View Details [407..758] deduced N/A No confident structure predictions are available.
    5 View Details [759..853] PSI-BLAST 375.64593 Anaphase promoting complex (APC)

    Functions predicted (by domain):

    # Gene Ontology predictions
    1
    Term Confidence Notes
  • ubiquitin-protein ligase activity
    		•  9.47178230940453 bayes_pls_golite062009
  • small conjugating protein ligase activity
    		•  9.15192834197166 bayes_pls_golite062009
  • acid-amino acid ligase activity
    		•  8.12051073361876 bayes_pls_golite062009
  • ligase activity, forming carbon-nitrogen bonds
    		•  6.87003086224111 bayes_pls_golite062009
  • ligase activity
    		•  4.85660414833673 bayes_pls_golite062009
  • transcription regulator activity
    		•  4.44907471960058 bayes_pls_golite062009
  • transcription factor activity
    		•  3.48782936574155 bayes_pls_golite062009
  • transcription repressor activity
    		•  2.39237599101586 bayes_pls_golite062009
  • binding
    		•  2.3219000606044 bayes_pls_golite062009
  • transcription activator activity
    		•  1.79615705315806 bayes_pls_golite062009
  • nucleic acid binding
    		•  1.31024813110778 bayes_pls_golite062009
  • RNA polymerase II transcription factor activity
    		•  1.2152419300113 bayes_pls_golite062009
  • protein binding
    		•  1.15192193279649 bayes_pls_golite062009
  • DNA binding
    		•  1.05574145691051 bayes_pls_golite062009
  • vasopressin activated calcium mobilizing receptor activity
    		•  0.416181464241621 bayes_pls_golite062009
  • transcription factor binding
    		•  0.31161141447305 bayes_pls_golite062009
  • catalytic activity
    		•  0.201606344181603 bayes_pls_golite062009
  • hydrolase activity
    		•  0.140191206949582 bayes_pls_golite062009
  • specific transcriptional repressor activity
    		•  0.0565989703351002 bayes_pls_golite062009
    2 No functions predicted.
    3 No functions predicted.
    4 No functions predicted.
    5 No functions predicted.


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.99

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle