Protein Overview: MYO3

General Information:
Name(s) found:	MYO3 / YKL129C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6322720\|ref\|NP_012793.1\| One of two type I myosins; localizes to actin cortical patches; deletion of MYO3 has little effect on growth, but myo3 myo5 double deletion causes severe defects in growth and actin cytoskeleton organization; Myo3p [Saccharomyc [NCBI NR] pir\|\|S37958 myosin heavy chain homolog YKL129c - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|486219\|emb\|CAA81970.1\| MYO3 [Saccharomyces cerevisiae] [NCBI NR] gi\|190358924\|sp\|P36006.3\|MYO3_YEAST RecName: Full=Myosin-3; AltName: Full=Class I unconventional myosin MYO3; AltName: Full=Type I myosin MYO3; AltName: Full=Actin-dependent myosin-I MYO3 [NCBI NR] Myosin-3 OS=Saccharomyces cerevisiae GN=MYO3 PE=1 SV=3 [Swiss-Prot] MYO3 Myosin type I [mips] One of two type I myosins; localizes to actin cortical patches; deletion of MYO3 has little effect on growth, but myo3 myo5 double deletion causes severe defects in growth and actin cytoskeleton organization [SGD] MYO3, Chr XI from 196347-200162, reverse complement [yeast_orfs2] Chr XI from 196347-200162, reverse complement [yeastorf3.fasta] Chr XI from 196346-200161, reverse complement [yeastorf4.fasta] Chr XI from 200161-196346, reverse complement [yeastorf5.fasta] MYO3 SGDID:S0001612, Chr XI from 200165-196350, reverse complement, Verified ORF [yeastflysequest.fasta] Myo3p [Saccharomyces cerevisiae]gi\|486219\|emb\|CAA81970.1\| MYO3 [Saccharomyces cerevisiae]gi\|539298\|pir\|\|S37958 myosin heavy chain homolog YKL129c - yeast (Saccharomyces cerevisiae) [nrdb_11152004_Chlamydomonas] MYO3 SGDID:S000001612, Chr XI from 200163-196348, reverse complement, Verified ORF, "One of two type I myosins; localizes to actin cortical patches; deletion of MYO3 has little effect on growth, but myo3 myo5 double deletion causes severe defects in growt [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] One of two type I myosins; localizes to actin cortical patches; deletion of MYO3 has little effect on growth, but myo3 myo5 double deletion causes severe defects in growth and actin cytoskeleton organization; Myo3p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] MYO3 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] MYO3 SGDID:S000001612, Chr XI from 200163-196348, reverse complement, Verified ORF, "One of two type I myosins; localizes to actin cortical patches; deletion of MYO3 has little effect on growth, but myo3 myo5 double deletion causes severe defects in growth and actin cytoskeleton organization" [yeast-bovrabprots.fasta] [chemostatdb.fasta]
Organism:	Saccharomyces cerevisiae
Length:	1271 amino acids
Gene Ontology:
Cellular Component:	actin cortical patch [IPI]
Biological Process:	bipolar cellular bud site selection [IMP] response to osmotic stress [IMP] cellular cell wall organization [IMP] endocytosis [IMP] exocytosis [IMP]
Molecular Function:	microfilament motor activity [TAS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MAVIKKGARR KDVKEPKKRS AKIKKATFDA NKKKEVGISD LTLLSKISDE SINENLKKRF  60
   61 KNGIIYTYIG HVLISVNPFR DLGIYTNAVL ESYKGKNRLE VPPHVFAIAE SMYYNLKSYN 120
  121 ENQCVIISGE SGAGKTEAAK RIMQYIAAAS NSHSESIGKI KDMVLATNPL LESFGCAKTL 180
  181 RNNNSSRHGK YLEIKFNSQF EPCAGNITNY LLEKQRVVGQ IKNERNFHIF YQFTKGASDT 240
  241 YKQMFGVQMP EQYIYTAAAG CTSADQLMRK DYEGTLEAMR TIGLVQEEQD QIFRMLAAIL 300
  301 WIGNISFIEN EEGNAQVGDT SVTDFVAYLL QVDASLLVKC LVERIMQTSH GMKRGSVYHV 360
  361 PLNPVQATAV RDALAKAIYN NLFDWIVDRV NVSLQAFPGA DKSIGILDIY GFEIFEHNSF 420
  421 EQICINYVNE KLQQIFIQLT LKAEQETYER EKIKWTPIKY FDNKVVCDLI EAKNPPGILA 480
  481 AMNDSIATAH ADSNAADQAF AQRLNLFNSN PYFELRANKF VIKHYAGDVT YDINGITDKN 540
  541 KDQLQKDLIE LIGTTTNTFL STIFPDDVDK DSKRRPPTAG DKIIKSANEL VETLSKAEPS 600
  601 YIRTIKPNQT KSPNDYDDHQ VLHQVKYLGL QENVRIRRAG FAYRQTFEKF VERFYLLSPD 660
  661 CSYAGDYTWD GDTLEAVKLI LRDAMIPEKE FQLGVTSVFI KTPESLFALE DMRDKYWYNM 720
  721 AARIQRAWRR FLQRRIDAAI KIQRTIREKK GGNKYVKLRD YGTKLLAGKK ERRSMSLLGY 780
  781 RAFMGDYLSC NESKTKGSYI RRQVGIKDKV VFSIKGECLH SKFGRSAQRL KKVFILTKKT 840
  841 FYIIGQTREQ NAMKYTQDYK IDVGKIKQVS LTNLQDDWMG VILVNSTQSD PLINTPFKTE 900
  901 LMTRLKKLNE KIMIKVGPTI EYHKQPNKLH TVRSKISDSA PKYGDIYKSS TIYVRRGHPA 960
  961 NSKSNKKPKN PGGLSGKPIK SKKSKHKSTH KHTHSHRSHR DAAKKQPLPS QKPVNPLSLR1020
 1021 ATAAQAAYNP KPDKTVPIKS SAIPAAKVSS KHSSKPSSKE KVAVKKASSS HKSSSAKQNQ1080
 1081 VSMPPSKGVE KNKEPLKETT ATATANIPIP PPPPPMGQPK DPKFEAAYDF PGSGSSSELP1140
 1141 LKKGDIVFIS RDEPSGWSLA KLLDGSKEGW VPTAYMTPYK DTRNTVPVAA TGAVNDVTNQ1200
 1201 KSSQIDNTIS SAQEGVQFGS ATVGPTSDNQ SNPVGTFSDG LASALAARAN KMRAESADDD1260
 1261 DNDDGDDDDD W

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		CMD1 KEL2 MYO3
View Details	(MIPS) Mewes HW, et al. (2004)		MYO1 MYO2 MYO3 MYO4 MYO5 SMY1 SMY2
View Details	Krogan NJ, et al. (2006)		CMD1 CMK1 CMK2 CMP2 CNA1 CNB1 MYO3 MYO5 YGL242C
View Details	Ho Y, et al. (2002)		CMD1 CMP2 HUL5 MLC1 MYO2 MYO3 MYO4 MYO5 SHE4 SPC110
View Details	Qiu et al. (2008)		ABP1 AIP1 BZZ1 CAP1 CAP2 DYN1 MYO1 MYO2 MYO3 MYO4 MYO5 PAN1 RVS167 SLA1 SRV2 TWF1 VRP1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	DAD1	Dam1-765 complex purified from yeast with Dad1-TAP	Shimogawa MM, et al. (2006)
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	MLP2	#03 Alpha Factor Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#29 Asynchronous Prep5-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#12 Mitotic Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#11 Mitotic Prep1-TiO2 enriched, new search criteria	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	UBC6	MYO3	1	Unpublished Fields Lab Data
View Screen	LSM8	MYO3	1	Unpublished Fields Lab Data

Microscopy / Localization Data

New Feature: Upload Your Own Microscopy Data

	PROTEIN(S)	PUBLICATION
View Data	MYO3	Huh WK, et al. (2003)
View Data	MYO3 and SAC6	Huh WK, et al. (2003)

Protein Structure Data

[What does the above image mean?]
[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..641]	PSI-BLAST	2600.0	Myosin S1, motor domain
2	View Details	[642..713]	PSI-BLAST	2600.0	Myosin S1, motor domain
3	View Details	[714..776]	PSI-BLAST	12.69897	SCALLOP MYOSIN REGULATORY DOMAIN
4	View Details	[777..1097]	PSI-BLAST	5.522879	Fibrinogen
5	View Details	[1098..1189]	PSI-BLAST	11.154902	Growth factor receptor-bound protein 2 (GRB2), N- and C-terminal domains
6	View Details	[1190..1271]	deduced	N/A	No confident structure predictions are available.

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
actin-dependent ATPase activity	5.83616693706338	bayes_pls_golite062009
microfilament motor activity	5.82833334737708	bayes_pls_golite062009
microtubule motor activity	4.51968578934603	bayes_pls_golite062009
motor activity	4.04927796049806	bayes_pls_golite062009
actin binding	3.33639699088484	bayes_pls_golite062009
transporter activity	3.10463051760206	bayes_pls_golite062009
transmembrane transporter activity	2.95886887664984	bayes_pls_golite062009
nucleoside-triphosphatase activity	2.95079405147356	bayes_pls_golite062009
pyrophosphatase activity	2.87427509025394	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides	2.85499564954033	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	2.85178667290149	bayes_pls_golite062009
tubulin binding	2.67743463093593	bayes_pls_golite062009
binding	2.64537357529343	bayes_pls_golite062009
primary active transmembrane transporter activity	2.61775212528604	bayes_pls_golite062009
P-P-bond-hydrolysis-driven transmembrane transporter activity	2.61467977505707	bayes_pls_golite062009
microtubule binding	2.59155206524122	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances	2.57552435627908	bayes_pls_golite062009
ATPase activity, coupled to transmembrane movement of substances	2.57107347424517	bayes_pls_golite062009
ATPase activity, coupled to movement of substances	2.57054427875021	bayes_pls_golite062009
hydrolase activity	2.55157966585974	bayes_pls_golite062009
active transmembrane transporter activity	2.50494515048649	bayes_pls_golite062009
ATPase activity, coupled	2.43862163407966	bayes_pls_golite062009
ADP binding	2.42564874799906	bayes_pls_golite062009
cytoskeletal protein binding	2.4101693596493	bayes_pls_golite062009
ATPase activity	2.39535714892224	bayes_pls_golite062009
actin filament binding	2.24723363507804	bayes_pls_golite062009
myosin light chain binding	2.23966366728473	bayes_pls_golite062009
substrate-specific transporter activity	1.91091452720653	bayes_pls_golite062009
alpha-tubulin binding	1.792591628112	bayes_pls_golite062009
substrate-specific transmembrane transporter activity	1.56325751172863	bayes_pls_golite062009
signal recognition particle binding	1.51332809264918	bayes_pls_golite062009
plus-end-directed microtubule motor activity	1.38366133153678	bayes_pls_golite062009
protein binding	1.22129154759482	bayes_pls_golite062009
catalytic activity	1.00104173493322	bayes_pls_golite062009
ion transmembrane transporter activity	0.977237423302079	bayes_pls_golite062009
structural constituent of muscle	0.960508657568442	bayes_pls_golite062009
purine nucleotide binding	0.929901285538132	bayes_pls_golite062009
nucleotide binding	0.922442819194247	bayes_pls_golite062009
purine ribonucleotide binding	0.920987930180051	bayes_pls_golite062009
ribonucleotide binding	0.920926158369519	bayes_pls_golite062009
GTPase activity	0.83849854735032	bayes_pls_golite062009
nucleic acid binding	0.775560967092349	bayes_pls_golite062009
beta-tubulin binding	0.654735466720932	bayes_pls_golite062009
guanyl nucleotide binding	0.640772201693186	bayes_pls_golite062009
guanyl ribonucleotide binding	0.63468044350616	bayes_pls_golite062009
GTP binding	0.621689261700018	bayes_pls_golite062009
cation transmembrane transporter activity	0.557919489256916	bayes_pls_golite062009
protein transporter activity	0.498432226280822	bayes_pls_golite062009
microtubule-severing ATPase activity	0.491769258942331	bayes_pls_golite062009
DNA binding	0.479412476059825	bayes_pls_golite062009
ATP binding	0.41092997612902	bayes_pls_golite062009
adenyl ribonucleotide binding	0.385813421701492	bayes_pls_golite062009
adenyl nucleotide binding	0.377870145186619	bayes_pls_golite062009
minus-end directed microfilament motor activity	0.329356138573379	bayes_pls_golite062009
plus-end directed microfilament motor activity	0.329356138573379	bayes_pls_golite062009
transcription regulator activity	0.312171320541728	bayes_pls_golite062009
signal transducer activity	0.286367085584159	bayes_pls_golite062009
molecular transducer activity	0.286367085584159	bayes_pls_golite062009
myosin binding	0.274885859454447	bayes_pls_golite062009
hydrogen ion transporting ATP synthase activity, rotational mechanism	0.213018989352421	bayes_pls_golite062009
G-protein coupled receptor activity	0.169034229773346	bayes_pls_golite062009
inorganic cation transmembrane transporter activity	0.123304946374679	bayes_pls_golite062009
metal ion transmembrane transporter activity	0.0775601177068852	bayes_pls_golite062009
structural constituent of cytoskeleton	0.0111338193708552	bayes_pls_golite062009
structural constituent of ribosome	0.0102645529518819	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.98

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2