Protein Overview: CAB3

General Information:
Name(s) found:	CAB3 / YKL088W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6322762\|ref\|NP_012835.1\| Predicted phosphopantothenoylcysteine decarboxylase, may be involved in coenzyme A biosynthesis; interacts with Sis2p and Vhs3p; Ykl088wp [Saccharomyces cerevisiae] [NCBI NR] gi\|549703\|sp\|P36076.1\|YKI8_YEAST RecName: Full=Uncharacterized protein YKL088W [NCBI NR] pir\|\|S37913 hypothetical protein YKL088w - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|486131\|emb\|CAA81926.1\| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR] Uncharacterized protein YKL088W OS=Saccharomyces cerevisiae GN=YKL088W PE=1 SV=1 [Swiss-Prot] Protein of unknown function localised to cytoplasm [mips] Subunit of a phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis; null mutant lethality is complemented by E. coli coaBC [SGD] YKL088W, Chr XI from 274925-276640 [yeast_orfs2] Chr XI from 274925-276640 [yeastorf3.fasta] Chr XI from 274924-276639 [yeastorf4.fasta] (P36076) Hypothetical 65.2 kDa protein in MIF2-CYT2 intergenic region [4932.FASTACCERV.txt] YKL088W SGDID:S0001571, Chr XI from 274928-276643, Uncharacterized ORF [yeastflysequest.fasta] Protein required for cell viability [Saccharomyces cerevisiae]gi\|486131\|emb\|CAA81926.1\| unnamed protein product [Saccharomyces cerevisiae]gi\|549703\|sp\|P36076\|YKI8_YEAST Hypothetical protein YKL088wgi\|539185\|pir\|\|S37913 hypothetical protein YKL088w - ye [nrdb_11152004_Chlamydomonas] (P36076) Hypothetical protein YKL088w [cerv4932.FASTAC] YKL088W SGDID:S000001571, Chr XI from 274927-276642, Uncharacterized ORF, "Predicted phosphopantothenoylcysteine decarboxylase, may be involved in coenzyme A biosynthesis; interacts with Sis2p and Vhs3p" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] CAB3 SGDID:S000001571, Chr XI from 274927-276642, Verified ORF, "Probable phosphopantothenoylcysteine decarboxylase (PPCDC), which catalyzes the third step of coenzyme A biosynthesis from pantothenate; null mutant lethality is complemented by E. coli coaB [SGD_S-cerevisiae_na_05-08-2009_con_reversed.fasta] Uncharacterized protein YKL088W [nr-271106-contam.fasta] unnamed protein product [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Predicted phosphopantothenoylcysteine decarboxylase, may be involved in coenzyme A biosynthesis; interacts with Sis2p and Vhs3p; Ykl088wp [Saccharomyces cerevisiae] [nr-271106-contam.fasta] CAB3 SGDID:S000001571, Chr XI from 274927-276642, Verified ORF, "Subunit of a phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis; null mutant lethality is complemented [SGD_S-cerevisiae_na_01-05-2010_reversed.fasta] CAB3 SGDID:S000001571, Chr XI from 274927-276642, Verified ORF, "Probable phosphopantothenoylcysteine decarboxylase (PPCDC), which catalyzes the third step of coenzyme A biosynthesis from pantothenate; null mutant lethality is complemented by E. coli coaBC; interacts with Sis2p and Vhs3p" [scerevisihumanrev.fasta] [chemostatdb.fasta] CAB3 SGDID:S000001571, Chr XI from 274927-276642, Verified ORF, "Subunit of a phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis; null mutant lethality is complemented by E. coli coaBC" [SGDyeast.20101221.fasta] CAB3 SGDID:S000001571, Chr XI from 275283-276998, Genome Release 64-1-1, Verified ORF, "Subunit of a phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis; null mutant lethality is complemented by E. coli coaBC" [yeast-030211-contam.fasta] CAB3 SGDID:S000001571, Chr XI from 275283-276998, Genome Release 64-2-1, Verified ORF, "Subunit of PPCDC and CoA-SPC complexes involved in CoA biosynthesis; subunits of the phosphopantothenoylcysteine decarboxylase (PPCDC) complex are: Cab3p, Sis2p, Vhs3p, while the subunits of the CoA-synthesizing protein complex (CoA-SPC) are: Cab2p, Cab3p, Cab4p, and Cab5p as well as Sis2p and Vhs3p; null mutant lethality is complemented by E. coli coaBC" [20141208_orf_trans.fasta] YKL088W SGDID:S000001571, Chr XI from 275283-276998, Genome Release 64-1-1, Verified ORF, "Subunit of a phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis; null mutant lethality is complemented by E. coli coaBC" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	571 amino acids
Gene Ontology:
Cellular Component:	cytoplasm [IDA ]
Biological Process:	response to salt stress [IGI] coenzyme A biosynthetic process [IPI][ISS]
Molecular Function:	phosphopantothenoylcysteine decarboxylase activity [ISS ] purine nucleotide binding [ISS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MTDEKVNSDQ NMNGKQGVNL ISSLPTTQVP VSILTNKERR KSIHDESNFE RSDSHEDQSK  60
   61 SNSNRRNIYK NDYSTNLRDF SFANLKQNSE RNKDGHEIQI NTSMPANTNG QQKRFSPSLP 120
  121 SAVSFTVPEV ERLPYHRYSI SNKPGKQQQQ QEQLQQNQQQ EEQQKAQLQE QNQRAKQQEE 180
  181 VKQIQEQVQK KQTERQQLID EKERIANAIF KENTTNDGTD IRKHSVSSGT SNSEDEVDSP 240
  241 SMEKNSIVHM PGDFIYFNPK SNASKPITAK AAPLSANNST HKNKEVITAP TGPRVPFTEF 300
  301 FQKEDDKKFH ILIGATGSVA TIKVPLIIDK LFKIYGPEKI SIQLIVTKPA EHFLKGLKMS 360
  361 THVKIWREED AWVFDAVNKN DTSLSLNLIL HHELRKWADI FLIAPLSANT LAKLANGICN 420
  421 NLLTSVMRDW SPLTPVLIAP AMNTFMYINP MTKKHLTSLV QDYPFIQVLK PVEKVLICGD 480
  481 IGMGGMREWT DIVEIVRRRI NEIRKARDEE TGDKEQEQEE QEGADNEDDD DEDDEEDEED 540
  541 EEEEEALNET ASDESNDEED EEDEEDVKTE V

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		CAB3 CKA1 CKA2 CKB2 SIS2 VHS3
View Details	Hazbun TR, et al. (2003)		CAB3 SIS2 VHS3
View Details	Krogan NJ, et al. (2006)		CAB3 CHD1 CKA1 CKA2 CKB1 CKB2 ELF1 HOT1 MSC3 NRG1 PGA2 REH1 RIM13 VHS3 YLR407W
View Details	Gavin AC, et al. (2006)		CAB3 PPZ1
View Details	Gavin AC, et al. (2002)		CAB3 CHD1 CKA1 CKA2 CKB1 CKB2 HHF1, HHF2 HOT1 ISW1 ISW2 NIP1 PRT1 RET1 RPG1 RVB2 SPT16 SSA4 TIF35 UTP22 VPS1 YTA7

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	CAB3	No Comments	Hazbun TR, et al. (2003)
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	CAB3	RFC2	2	Hazbun TR, et al. (2003)
View Screen	CAB3	PPZ2	2	Unpublished Fields Lab Data
View Screen	CAB3	GIS3	2	Unpublished Fields Lab Data
View Screen	CAB3	ATG11	2	Hazbun TR, et al. (2003)
View Screen	CAB3	SML1	2	Unpublished Fields Lab Data
View Screen	CAB3	JNM1	2	Hazbun TR, et al. (2003)
View Screen	CAB3	ATG11	2	Hazbun TR, et al. (2003)
View Screen	CAB3	JNM1	2	Hazbun TR, et al. (2003)
View Screen	CAB3	YCL020W	2	Hazbun TR, et al. (2003)
View Screen	CAB3	PET494	2	Unpublished Fields Lab Data
View Screen	CAB3	EPS1	2	Hazbun TR, et al. (2003)
View Screen	CAB3	RFC2	2	Unpublished Fields Lab Data
View Screen	CAB3	WHI4	2	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	CAB3 and SPC42	Hazbun TR, et al. (2003)
View Data	CAB3 and SPC42	Unpublished Data
View Data	CAB3	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..275]	Pcons	1.58	Tropomyosin
2	View Details	[276..571]	PSI-BLAST	405.228787	4'-phosphopantothenoylcysteine decarboxylase (PPC decarboxylase, halotolerance protein Hal3a)

Functions predicted (by domain):

Gene Ontology predictions

No functions predicted.

Term	Confidence	Notes
phosphopantothenoylcysteine decarboxylase activity	3.86093811191169	bayes_pls_golite062009
phosphopantothenate--cysteine ligase activity	2.64345867885176	bayes_pls_golite062009
carboxy-lyase activity	1.79168084682693	bayes_pls_golite062009
catalytic activity	1.51672397362026	bayes_pls_golite062009
carbon-carbon lyase activity	1.03827559540394	bayes_pls_golite062009
binding	0.954975261385974	bayes_pls_golite062009
protein binding	0.259532204752794	bayes_pls_golite062009

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.94

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2